Physiological impact and comparison of mutant screening methods in piwil2 KO founder Nile tilapia produced by CRISPR/Cas9 system

Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9-mediated HDR in Atlantic salmon

(2020) Anne Hege Straume et al.   Scientific Reports

Production of a breed of red sea bream Pagrus major with an increase of skeletal muscle mass and reduced body length by genome editing with CRISPR/Cas9

(2018) Kenta Kishimoto et al.   AQUACULTURE

A Rapid CRISPR/Cas-based Mutagenesis Assay in Zebrafish for Identification of Genes Involved in Thyroid Morphogenesis and Function

(2018) A. Trubiroha et al.   Scientific Reports

A Survey of Validation Strategies for CRISPR-Cas9 Editing

(2018) Monica F. Sentmanat et al.   Scientific Reports

Not just for the wealthy: Rethinking farmed fish consumption in the Global South

(2018) Ben Belton et al.   Global Food Security-Agriculture Policy Economics and Environment

Editing the duplicated insulin-like growth factor binding protein-2b gene in rainbow trout (Oncorhynchus mykiss)

(2018) Beth M. Cleveland et al.   Scientific Reports

Mosaicism in CRISPR/Cas9-mediated genome editing

(2018) Maryam Mehravar et al.   DEVELOPMENTAL BIOLOGY

Predictable and precise template-free CRISPR editing of pathogenic variants

(2018) Max W. Shen et al.   NATURE

Expression pattern of nanos, piwil, dnd, vasa and pum genes during ontogenic development in Nile tilapia Oreochromis niloticus

(2018) Y.H. Jin et al.   GENE

Generation of biallelic F0 mutants in medaka using the CRISPR/Cas9 system

(2017) Rie Sawamura et al.   GENES TO CELLS

Temperature effect on CRISPR-Cas9 mediated genome editing

(2017) Guanghai Xiang et al.   Journal of Genetics and Genomics

Production of Tiger Puffer Takifugu rubripes Offspring from Triploid Grass Puffer Takifugu niphobles Parents

(2017) Masaomi Hamasaki et al.   MARINE BIOTECHNOLOGY

Heterozygous mutation of eEF1A1b resulted in spermatogenesis arrest and infertility in male tilapia, Oreochromis niloticus

(2017) Jinlin Chen et al.   Scientific Reports

A simplified method for identifying early CRISPR-induced indels in zebrafish embryos using High Resolution Melting analysis

(2016) Éric Samarut et al.   BMC GENOMICS

Haploinsufficiency of SF-1 Causes Female to Male Sex Reversal in Nile Tilapia,Oreochromis niloticus

(2016) Qing-Ping Xie et al.   ENDOCRINOLOGY

Characterization of a vasa homolog in the brown-marbled grouper (Epinephelus fuscoguttatus) and its expression in gonad and germ cells during larval development

(2016) Surintorn Boonanuntanasarn et al.   FISH PHYSIOLOGY AND BIOCHEMISTRY

Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders

(2016) Mitsuki Shigeta et al.   GENES TO CELLS

Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Zinc Finger Nuclease Technology with Electroporation

(2016) Zhenkui Qin et al.   MARINE BIOTECHNOLOGY

gsdf is a downstream gene of dmrt1 that functions in the male sex determination pathway of the Nile tilapia

(2016) Dong-Neng Jiang et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Complete Spectrum of CRISPR/Cas9-induced Mutations on HBV cccDNA

(2016) Christoph Seeger et al.   MOLECULAR THERAPY

Analyzing CRISPR genome-editing experiments with CRISPResso

(2016) Luca Pinello et al.   NATURE BIOTECHNOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon

(2016) Anna Wargelius et al.   Scientific Reports

CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations

(2016) Changtian Pan et al.   Scientific Reports

Disruption of Zebrafish Follicle-Stimulating Hormone Receptor (fshr) But Not Luteinizing Hormone Receptor (lhcgr) Gene by TALEN Leads to Failed Follicle Activation in Females Followed by Sexual Reversal to Males

(2015) Zhiwei Zhang et al.   ENDOCRINOLOGY

Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale

(2015) Ai Sun et al.   FISH PHYSIOLOGY AND BIOCHEMISTRY

Primordial germ cell migration in the yellowtail kingfish (Seriola lalandi) and identification of stromal cell-derived factor 1

(2015) J.A. Fernández et al.   GENERAL AND COMPARATIVE ENDOCRINOLOGY

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants

(2015) Xingliang Ma et al.   Molecular Plant

Fast and sensitive detection of indels induced by precise gene targeting

(2015) Zhang Yang et al.   NUCLEIC ACIDS RESEARCH

CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity

(2015) Blake Carrington et al.   NUCLEIC ACIDS RESEARCH

High-throughput genotyping of CRISPR/Cas9-mediated mutants using fluorescent PCR-capillary gel electrophoresis

(2015) Muhammad Khairul Ramlee et al.   Scientific Reports

A Tandem Duplicate of Anti-Müllerian Hormone with a Missense SNP on the Y Chromosome Is Essential for Male Sex Determination in Nile Tilapia, Oreochromis niloticus

(2015) Minghui Li et al.   PLoS Genetics

Retinoic acid homeostasis through aldh1a2 and cyp26a1 mediates meiotic entry in Nile tilapia (Oreochromis niloticus)

(2015) Ruijuan Feng et al.   Scientific Reports

Efficient and Heritable Gene Targeting in Tilapia by CRISPR/Cas9

(2014) Minghui Li et al.   GENETICS

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

(2014) Xuebing Wu et al.   NATURE BIOTECHNOLOGY

Microhomology-based choice of Cas9 nuclease target sites

(2014) Sangsu Bae et al.   NATURE METHODS

CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos

(2014) Yanjiao Shao et al.   Nature Protocols

Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 Generation

(2014) Rolf B. Edvardsen et al.   PLoS One

High-Throughput Genome Editing and Phenotyping Facilitated by High Resolution Melting Curve Analysis

(2014) Holly R. Thomas et al.   PLoS One

Genotyping with CRISPR-Cas-derived RNA-guided endonucleases

(2014) Jong Min Kim et al.   Nature Communications

Intraperitoneal Germ Cell Transplantation in the Nile Tilapia Oreochromis niloticus

(2013) Rodolfo Farlora et al.   MARINE BIOTECHNOLOGY

Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

(2013) Li-En Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Medaka piwi is Essential for Primordial Germ Cell Migration

(2012) M. Li et al.   CURRENT MOLECULAR MEDICINE

Germ cells are not the primary factor for sexual fate determination in goldfish

(2012) Rie Goto et al.   DEVELOPMENTAL BIOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome

(2012) Timothy J. Dahlem et al.   PLoS Genetics

Specific expression of Olpiwi1 and Olpiwi2 in medaka (Oryzias latipes) germ cells

(2011) Haobin Zhao et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Recognition of 2′-O-Methylated 3′-End of piRNA by the PAZ Domain of a Piwi Protein

(2011) Bernd Simon et al.   STRUCTURE

Assessing impacts of introduced aquaculture species on native fish communities: Nile tilapia and major carps in SE Asian freshwaters

(2009) Robert I. Arthur et al.   AQUACULTURE

Zili is required for germ cell differentiation and meiosis in zebrafish

(2008) Saskia Houwing et al.   EMBO JOURNAL

Sexual Dimorphic Expression of Genes in Gonads During Early Differentiation of a Teleost Fish, the Nile Tilapia Oreochromis niloticus1

(2007) Shigeho Ijiri et al.   BIOLOGY OF REPRODUCTION