Article
Endocrinology & Metabolism
Dong-Neng Jiang, You-Xing Peng, Xing-Yong Liu, Umar Farouk Mustapha, Yuan-Qing Huang, Hong-Juan Shi, Ming-Hui Li, Guang-Li Li, De-Shou Wang
Summary: This study investigates the function of Gsdf in fish ovary development using CRISPR/Cas9 technology. The results indicate that Gsdf functions together with the TGF-beta signaling pathway to control ovary development and fertility. Mutated ovaries show hyperplasia, but differential expression of key genes involved in ovary development is observed.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Fisheries
Sen Fan, Hong -Juan Shi, You -Xing Peng, Yuan-Qing Huang, Mou-Yan Jiang, Guang-Li Li, De-Shou Wang, Dong-Neng Jiang
Summary: Gonadal soma-derived factor (gsdf) is critical for sex determination and differentiation in fish, but its functions in spermatogenesis are unclear. This study found that XY gsdf/Nile tilapia can be converted to males by inhibiting estrogen synthesis, and these male fish are infertile. Transcriptome analysis revealed dysregulation of genes related to the endocrine system and azoospermia in these infertile fish. These findings provide a foundation for future research on the role of gsdf in spermatogenesis in fish.
Article
Veterinary Sciences
Huan Zhong, Zhongbao Guo, Jun Xiao, Hong Zhang, Yongju Luo, Junneng Liang
Summary: We investigated the expression profiles and regulatory mechanisms of circRNAs in fish gonad development. Tilapia, which is an important species for economic and sexual selection studies, was used as the research object. Through high-throughput deep sequencing and circRNA-specific computing tools, we detected the expression of circRNAs in the ovary, testis, and sex-changed gonad of tilapia. Our findings provide new evidence for understanding the circRNA-miRNA networks in tilapia gonads.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemical Research Methods
Xinting Zhang, Yuyang Chang, Wanying Zhai, Feng Qian, Yingqing Zhang, Shumei Xu, Haiyan Guo, Siyu Wang, Ruiqin Hu, Xiaozhu Zhong, Xiaomiao Zhao, Liangbiao Chen, Guijun Guan
Summary: Gsdf interacts with various proteins, promoting protein synthesis and ribosomal biogenesis to drive the differentiation of undifferentiated germ cells. This interaction plays a crucial role in the basic processes of germ cell proliferation, transcriptional and translational control of sexual RNA, potentially conserved across different species during sexual differentiation.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Multidisciplinary Sciences
Chor Hong Lim, Tomoko Soga, Ishwar S. Parhar
Summary: Social disturbance in interpersonal relationships is a primary source of stress in humans. This study investigated the regulatory relationship between the central 5-HT system and SPX1a gene expression in Nile tilapia. The results showed that 5-HT can down-regulate the expression of SPX1a, and socially defeated tilapia exhibited increased SPX1a expression and up-regulated 5-HT receptors. Citalopram treatment normalized SPX1a gene expression in socially defeated fish.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Marine & Freshwater Biology
Li-ping Cao, Jin-liang Du, Rui Jia, Wei-dong Ding, Pao Xu, Guo-jun Yin, Ting Zhang
Summary: The study found that cyclophosphamide treatment in fish induces cytotoxic effects on immune cells, lipid peroxidation, reduced antioxidant capacity, and inhibited immune function.
AQUATIC TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Michelle Thonnes, Rebecca Prause, Berta Levavi-Sivan, Frank Pfennig
Summary: African cichlids are important models for studying social hierarchies and gene expression. This study used RNA-sequencing to investigate the transcriptomes of testes and pituitaries in dominant and subordinate male Nile tilapia living in stable social hierarchies. Results showed differential gene expression between dominant and subordinate males, with upregulation of growth factors, signaling pathways, and genes related to gonadotropin and steroidogenesis in dominant males. Hormone levels were also higher in dominant males.
Article
Endocrinology & Metabolism
Lanying Yang, You Wu, Yun Su, Xuefeng Zhang, Tapas Chakraborty, Deshou Wang, Linyan Zhou
Summary: This study reveals the essentiality of the cyp17a2 gene in the production of cortisol and DHP and its role in meiotic initiation and subfertility. This highlights the importance of DHP and cortisol in male fertility in fish.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Immunology
Liangliang Mu, Xiaoxue Yin, Hao Bai, Jiadong Li, Li Qiu, Qingliang Zeng, Shengli Fu, Jianmin Ye
Summary: In this study, it was found that MBL can reduce the proliferation of activated macrophages by regulating the cell cycle and increasing the probability of apoptosis in Nile tilapia. The inhibition of cell proliferation by MBL was closely related to the TGF-beta 1 signaling pathway.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Zoology
Meng-Fei Zhang, Shi-Cheng Wan, Wen-Bo Chen, Dong-Hui Yang, Wen-Qing Liu, Ba-Lun Li, Aili Aierken, Xiao-Min Du, Yun-Xiang Li, Wen-Ping Wu, Xin-Chun Yang, Yu-Dong Wei, Na Li, Sha Peng, Xue-Ling Li, Guang-Peng Li, Jin-Lian Hua
Summary: Bacterial or viral infections can lead to spermatogenesis disorder and infertility by disrupting immune homeostasis of the testes. Recent research shows that SARS-CoV-2 can infect male gonads and cause male reproductive dysfunction. Finding alternative treatments for inflammatory injury is critical.
ZOOLOGICAL RESEARCH
(2023)
Article
Environmental Sciences
Samah S. Oda, El-Sayed M. El-Manakhly, Mohamed A. Abou-Srag, Hossam G. Tohamy
Summary: The study revealed that carbofuran and copper sulfate have a negative impact on the reproductive function of male Nile tilapia, with their combination showing a stronger harmful effect. Exposure to these chemicals led to testicular damage, reduced sperm quality and quantity in male Nile tilapia.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biology
Caihong Han, Qionglin Peng, Xiangbin Su, Limin Xing, Xiaoxiao Ji, Yufeng Pan
Summary: A new ancestral male-specific doublesex isoform, dsxM2, was identified and the splicing-based mechanism underlying sex-specific Dmrt function was explored. The study reveals the evolutionary pathway from sexually monomorphic to sex-specific doublesex.
COMMUNICATIONS BIOLOGY
(2022)
Article
Fisheries
Aulidya Nurul Habibah, Ahmad Reza Sharifi, Stephan Wessels, Joerg Wilting, Gabriele Hoerstgen-Schwark, Wolfgang Holtz
Summary: The study found that in Nile tilapia, thermal-treated females undergo masculinization, leading to increased body mass, without affecting ovarian dynamics in non-masculinized females. This may contribute to successful breeding of offspring and have a positive impact on global food supply.
Article
Environmental Sciences
Jian Teng, Yan Zhao, Hong Ju Chen, Liang Yi Xue, Xiang Shan Ji
Summary: This study investigated the potential effects of exposure to a low concentration of letrozole on the gonad transcriptome of Nile tilapia during early sexual development. The results indicated that letrozole exposure led to changes in sex development and affected key genes related to metabolism and epigenetic regulation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Fisheries
Minjun Xu, Jiawei Ye, Yujie Wang, Kejie Chu, Maxime Pere, Minjie Xu, Xuelian Tang, Jinghua Fu
Summary: This study investigated the relationship between VE and PAP in fish and uncovered the function of PAP. Both VE and PAP showed antioxidant effects in the liver and serum of Nile tilapia, increasing the activities of antioxidant enzymes and improving miRNA expression profiles. VE promoted the accumulation of PAP, which entered the nucleus to inhibit the expression of XRN genes, similar to its function in plants. These findings lay the foundation for the application of PAP as a new antioxidant agent in fish farming and provide further understanding of VE's antioxidant function in fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Marine & Freshwater Biology
Minghui Li, Shengfei Dai, Xingyong Liu, Hesheng Xiao, Deshou Wang
Summary: Reverse genetics approaches, especially CRISPR/Cas9, are essential for understanding complex biological processes and genetic engineering. Nile tilapia, with its XX/XY sex determination system, serves as a valuable model for studying fish sex determination and reproduction. The detailed protocol provided in this study will facilitate the application of CRISPR/Cas9 in tilapia research and aquaculture studies.
Article
Biochemistry & Molecular Biology
Wenjing Tao, Luohao Xu, Lin Zhao, Zexian Zhu, Xin Wu, Qianwen Min, Deshou Wang, Qi Zhou
Summary: Researchers have reported the newly produced genomes of Nile tilapia and blue tilapia, providing detailed insights into their sex chromosome turnover mechanisms and sex-determining pathways, which are crucial for the development of genetic sex control in aquaculture. The study suggests that unequal distribution of repeat content may impact the local recombination rate, making some chromosomes more likely to become sex chromosomes.
MOLECULAR ECOLOGY RESOURCES
(2021)
Article
Fisheries
Xin Wu, Lin Zhao, Zheng Fan, Baoyue Lu, Jinlin Chen, Dejie Tan, Dongneng Jiang, Wenjing Tao, Deshou Wang
Summary: The study bred WW super-females, identified structural variations in the sex determining region of blue tilapia, designed and screened three sex-linked markers, and successfully established a genetic sex identification technique. This is significant for studying sex determination genes, understanding sex chromosome evolution, and the transition of sex determination systems.
Article
Evolutionary Biology
Chenxu Wang, Baoyue Lu, Tao Li, Guangyuan Liang, Mengmeng Xu, Xingyong Liu, Wenjing Tao, Linyan Zhou, Thomas D. Kocher, Deshou Wang
Summary: The diverse color patterns of cichlid fishes are important in mate choice and speciation. Different genes control body and eye pigmentation in Nile tilapia. Several genes, including mitf, kita/kitlga, and pmel, are significant for color patterning in tilapia, making it an excellent model system for studying vertebrate color patterning.
JOURNAL OF HEREDITY
(2021)
Article
Biochemistry & Molecular Biology
Zhisheng Ma, Jing Yang, Qingqing Zhang, Chunmei Xu, Jing Wei, Lina Sun, Deshou Wang, Wenjing Tao
Summary: miR-133b, a member of the miR-133 family, plays a crucial role in tilapia oogenesis by regulating the expression of tagln2 gene and impacting estrogen synthesis. The study validates the role of miR-133b in fish reproduction through experiments in mice, laying the foundation for further exploration of the regulatory network in fish oogenesis.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Lanying Yang, Xuefeng Zhang, Shujun Liu, Chenhua Zhao, Yiyang Miao, Li Jin, Deshou Wang, Linyan Zhou
Summary: In teleost fish, the gene Cyp17a1 plays a crucial role in steroidogenesis, sex determination, and fertility. Mutations in the cyp17a1 gene in Nile tilapia led to female-to-male sex reversal and deficiencies in hormone production, impacting both the sex determination and reproductive processes in fish. Overall, Cyp17a1 is found to be essential for E2 production in female sex determination and differentiation, as well as for T and 11-KT production in promoting spermatogenesis and fertility in male fish.
Review
Endocrinology & Metabolism
Linyan Zhou, Minghui Li, Deshou Wang
Summary: The involvement of sex steroids in sex determination and differentiation in non-mammalian vertebrates, especially in fish, is relatively conserved. Advances in genome sequencing and editing have led to significant progress in understanding steroidogenic pathways and hormonal regulation of sex determination in fish. Gene editing studies challenge traditional views on the roles of estrogen and androgen in ovarian and testicular development, and highlight the critical role of estrogen in female sex determination.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lu Li, You Wu, Chenhua Zhao, Yiyang Miao, Jing Cai, Lingyun Song, Jing Wei, Tapas Chakraborty, Limin Wu, Deshou Wang, Linyan Zhou
Summary: The StAR2 gene plays a critical role in testicular development, spermatogenesis, and spermiation in tilapia by regulating androgen production. However, it may not be essential and could be compensated for in the absence of StAR2.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Xingyong Liu, Shengfei Dai, Jiahong Wu, Xueyan Wei, Xin Zhou, Mimi Chen, Dejie Tan, Deyong Pu, Minghui Li, Deshou Wang
Summary: The study analyzed the expression and functions of amh duplicates in Nile tilapia. It was found that amhy is expressed earlier and at a higher level during sex determination, and it determines sex by repressing the transcription of cyp19a1a. Mutations in amhy lead to sex reversal and altered germ cell proliferation.
Article
Evolutionary Biology
Chenxu Wang, Jia Xu, Thomas D. Kocher, Minghui Li, Deshou Wang
Summary: In this study, researchers identified two pmel genes in Nile tilapia and demonstrated their importance in pigmentation. Homozygous mutation of pmela resulted in a yellowish body color with weak vertical bars, while mutation of pmelb had a milder effect. Double mutation of both genes led to a decrease in melanophores and an increase in xanthophores, resulting in a golden body color. The findings provide new strategies for breeding golden tilapia and offer a new model for studying pmel function in vertebrates.
JOURNAL OF HEREDITY
(2022)
Article
Biology
Shuqing Zheng, Wenjing Tao, Haowen Yang, Thomas D. Kocher, Zhijian Wang, Zuogang Peng, Li Jin, Deyong Pu, Yaoguang Zhang, Deshou Wang
Summary: Teleosts are important models for studying sex chromosomes and sex-determining genes. In this study, a high-contiguity chromosome-level genome assembly on a YY southern catfish was generated using Nanopore and Hi-C technologies. The study identified a candidate sex-determining gene in the sex-determining region and confirmed the role of amhr2y in male sex determination.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Chunmei Xu, Miao Yu, Qingqing Zhang, Zhisheng Ma, Kang Du, Huiqin You, Jing Wei, Deshou Wang, Wenjing Tao
Summary: In this study, the BRD gene family was identified and characterized in Nile tilapia, revealing sexually dimorphic expression patterns and their critical role in spermatogenesis. The findings provide insights into the understanding of sex differentiation and gonadal development in teleosts.
Correction
Biology
Shuqing Zheng, Wenjing Tao, Haowen Yang, Thomas D. Kocher, Zhijian Wang, Zuogang Peng, Li Jin, Deyong Pu, Yaoguang Zhang, Deshou Wang
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Xingyong Liu, Li Zhou, Wenbo Li, Jiahong Wu, Deshou Wang
Summary: In this study, it was found that GATA2 plays a critical role in lens morphogenesis in Nile tilapia. Mutations in the gata2a gene led to degeneration and apoptosis of lens fiber cells, causing progressive microphthalmia and blindness in adulthood. Transcriptome analysis revealed significant changes in the expression levels of genes encoding crystallin and genes involved in visual perception and metal ion binding after gata2a mutation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Zeming Zhang, Jie Wang, Jianeng Li, Xiang Liu, Lei Liu, Changle Zhao, Wenjing Tao, Deshou Wang, Jing Wei
Summary: In this study, an integrated CRISPR/Cas9 system was established for gene knock-out and knock-in at a specific site in medaka. The system is simple, cost-effective, and efficient. The results showed that pCas9-mU6sgRNA driven by endogenous U6 promoter achieved high gene editing efficiency in medaka cultured cells, but not by exogenous U6 promoters. This study provides insights into other fish genome editing and promotes gene functional analysis.