Article
Multidisciplinary Sciences
Laurie J. Mitchell, Valerio Tettamanti, Justin S. Rhodes, N. Justin Marshall, Karen L. Cheney, Fabio Cortesi
Summary: A gene-editing protocol using the CRISPR/Cas9 system was developed for the false clown anemonefish, resulting in successful targeting of vision and melanin production-related genes with high mutation efficiency. This study demonstrates the potential of CRISPR/Cas9 for studying gene function in anemonefishes and other reef fishes.
Article
Agricultural Engineering
Chen Bai, Yingping Cao, Siyi Zhao, Zhenying Wu, Shaojun Dai, Honglun Wang, Chunxiang Fu
Summary: This study successfully established a genome editing platform for Monochasma savatieri for the first time using the Agrobacterium rhizogenes-mediated co-transformation system. This will enable the development of novel M. savatieri germplasm/cultivars for facility cultivation and exploitation of its medicinal value in the future.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Genetics & Heredity
Eva Louise Steinmetz, Sandra Noh, Christine Kloeppel, Martin F. Fuhr, Nicole Bach, Mona Evelyn Raffael, Kirsten Hildebrandt, Fabienne Wittling, Doris Jann, Uwe Walldorf
Summary: This study focused on mutants isolated from the 57B region of Drosophila melanogaster. Through EMS mutagenesis and gene targeting, mutants for multiple genes in the 57B region were obtained and their lethality periods were determined. These mutants can serve as valuable tools for further exploration of the functions of the corresponding genes.
Article
Multidisciplinary Sciences
Sameer Singh, Anoosha Banerjee, Arnaud Vanden Broeck, Sebastian Klinge
Summary: One of the challenges in CRISPR/Cas9 editing is the difficulty of rapidly selecting clonal populations of biallelically edited cells. This study presents a platform called SNEAK PEEC that combines human genome editing with cell-surface display, allowing the direct identification of biallelically edited clones with minimal screening.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Duran Zhao, Johanna L. Jones, Robert J. Gasperini, Jac C. Charlesworth, Guei-Sheung Liu, Kathryn P. Burdon
Summary: Identification of genes causing cataract is crucial for understanding the pathology of inherited pediatric cataract and developing new therapies. This study presents a step-by-step pipeline to evaluate cataract candidate genes in zebrafish using CRISPR-Cas9, offering a simple and efficient method for assessment in basic laboratory settings.
Article
Biology
Ali Seleit, Alexander Aulehla, Alexandre Paix
Summary: A simplified, highly efficient, and precise strategy for CRISPR/Cas9-mediated endogenous protein tagging was established in medaka. This method achieved high efficiency of F0 targeting and germline transmission, significantly expanding the repertoire of genetic tools available in medaka. The potential for organismal-wide label for proliferative zones and an endogenous cell cycle reporter was shown in the mScarlet-pcna line.
Article
Biochemistry & Molecular Biology
Me-Sun Kim, Seo-Rin Ko, Yu Jin Jung, Kwon-Kyoo Kang, Yung-Jo Lee, Yong-Gu Cho
Summary: Plants produce stress-resistant substances in response to abiotic stress, and a protein conversion mechanism helps break down damaged proteins. The study focused on the function of OsPUB7 gene in Oryza sativa and found that OsPUB7 plays a role in drought and salinity stress resistance. The interaction between OsPUB7 and other stress-related genes suggests its potential as a target for breeding and future research on drought tolerance in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Haniel Siqueira Mortagua Walflor, Aline Rodrigues Castro Lucena, Felipe Francisco Tuon, Lia Carolina Soares Medeiros, Helisson Faoro
Summary: Antimicrobial resistance is a major global public health concern and was identified as one of the main causes of death in 2019. This study successfully restored the sensitivity to fosfomycin using a CRISPR-Cas9 system and identified effective guide RNAs for resensitization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xinyi Li, Bing Sun, Hongrun Qian, Jinrong Ma, Magdalena Paolino, Zhiying Zhang
Summary: Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 nuclease (Cas9) is a highly efficient genome editing tool. However, achieving biallelic editing remains a technical challenge. In this study, the authors developed a novel method to enable biallelic editing using CRISPR/Cas9-mediated homology-directed repair (HDR), and enriched the positively targeted cell clones through an integrated selection system. Experimental results on the APP and PSEN1 genes demonstrated high biallelic editing efficiency using this approach.
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
(2022)
Article
Agronomy
Xiao Chen, Suxin Yang, Yaohua Zhang, Xiaobin Zhu, Xinjing Yang, Chunbao Zhang, Haiyan Li, Xianzhong Feng
Summary: Soybean provides a rich source of plant protein and oil worldwide, with the commercial use of transgenic technology in soybean as a classical example of biotechnology application to crop improvement. Hybrid soybean breeding is also a potential way to increase soybean yield, in addition to genetically modified soybeans.
Article
Biochemistry & Molecular Biology
Chengxi Sun, Chen Li, Wen Liu, Helgi B. Schioth
Summary: Humans and other organisms are exposed to thousands of chemicals, many of which are present in low concentrations and may have adverse effects on the circadian rhythm. Traditional toxicological methods are not effective for assessing complex mixtures of contaminants. This study establishes a cell line that can be used for high-throughput screening of chemicals that disrupt the circadian rhythm.
Article
Biochemistry & Molecular Biology
Xiao Hu, Fei Hao, Xiaocong Li, Zhiyuan Xun, Yuan Gao, Bingxu Ren, Ming Cang, Hao Liang, Dongjun Liu
Summary: In this study, a gene-edited cashmere goat with improved fiber length and yield was generated by integrating the VEGF gene at the FGF5 site. The up-regulation of VEGF and down-regulation of FGF5 affected cell cycle, proliferation, and vascular tone through the PI3K-AKT signaling pathway and extracellular matrix-receptor interactions, resulting in impressive cashmere performance of the gene-edited cashmere goat.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Fisheries
Eun Gyeong Lee, Jun Soung Kwak, Ki Hong Kim
Summary: In this study, a CRISPR/Cas9 system was used to produce auxotrophic E. piscicida mutants, which proved to be a simpler and faster method than the traditional allelic exchange method. The mutants showed superior protection against E. piscicida, but potential reversion was observed in some cases.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Xueqi Li, Rong Li, Zheng Yuan, Zaobing Zhu, Wenting Xu, Yijie Wang, Dabing Zhang, Litao Yang
Summary: In this study, a new method called Cc-qPCR was developed for rapid screening of mutants and identification of their genotypes, as well as evaluating gene editing efficiency. Testing on rice samples showed that Cc-qPCR is an accurate and effective approach, which can enhance the efficiency and range of molecular breeding techniques.
ANALYTICAL CHEMISTRY
(2022)
Article
Genetics & Heredity
Qiting Yao, Shi Duan, Qing Yang, Xiaoqin Ma, Zhenyu Li, Kongyue Wu, Pei Chang, Mengxi Cao, Xinhua Chen, Zequn Wang, Xueping Zhong, Qingchun Zhou, Haobin Zhao
Summary: This study reveals the critical role of Mep50 in medaka embryonic development. Loss of Mep50 leads to abnormal embryogenesis and affects the maintenance of stem cells and inhibition of apoptosis.
Article
Biochemistry & Molecular Biology
Shinpei Obata, Eri Yuasa, Daisuke Seki, Takeshi Kitano, Hisato Saitoh
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2013)
Article
Endocrinology & Metabolism
Norikazu Murozumi, Ryo Nakashima, Toshiaki Hirai, Yasuhiro Kamei, Tomoko Ishikawa-Fujiwara, Takeshi Todo, Takeshi Kitano
Article
Biochemistry & Molecular Biology
Takeshi Kitano, Yuki Hayashi, Eri Shiraishi, Yasuhiro Kamei
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2012)
Article
Multidisciplinary Sciences
Eri Shiraishi, Hamid Hosseini, Dong K. Kang, Takeshi Kitano, Hidenori Akiyama
Article
Multidisciplinary Sciences
Fumiya Furukawa, Shin Hamasaki, Seiji Hara, Tomoya Uchimura, Eri Shiraishi, Natsumi Osafune, Hisanori Takagi, Takashi Yazawa, Yasuhiro Kamei, Takeshi Kitano
SCIENTIFIC REPORTS
(2019)
Article
Endocrinology & Metabolism
Tomoya Uchimura, Seiji Hara, Takashi Yazawa, Yasuhiro Kamei, Takeshi Kitano
FRONTIERS IN ENDOCRINOLOGY
(2019)
Article
Multidisciplinary Sciences
Seiji Hara, Fumiya Furukawa, Koki Mukai, Takashi Yazawa, Takeshi Kitano
SCIENTIFIC REPORTS
(2020)
Article
Environmental Sciences
Xuchun Qiu, Takumi Takamura, Shintaro Enoki, Yoko Kato-Unoki, Yuki Takai, Yosuke Nagano, Masato Kinoshita, Takeshi Kitano, Yohei Shimasaki, Yuji Oshima
MARINE POLLUTION BULLETIN
(2020)
Article
Fisheries
Seiji Hara, Rie Sawamura, Takeshi Kitano
Summary: This study reveals that cortisol inhibits female-type proliferation of germ cells and induces masculinization of XX medaka through the actions of gonadal soma-derived growth factor (GSDF) and anti-Mullerian hormone receptor type 2 (AMHR2). Loss of GSDF or AMHR2 function under cortisol treatment leads to recovery of female-type proliferation in germ cells and prevents masculinization of XX medaka.
Article
Fisheries
Hiroyuki Inaba, Seiji Hara, Moemi Horiuchi, Shigeho Ijiri, Takeshi Kitano
Summary: The study revealed the essential role of estrogen in sex determination in Japanese eels, and provided the expression profiles of sex-specific genes (foxl2a, cyp19a1, amh, gsdf) during sexual differentiation, highlighting their importance in the process of sexual differentiation and development in the Japanese eel species.
Article
Cell Biology
Takeshi Kitano, Tomoaki Takenaka, Hisanori Takagi, Yasutoshi Yoshiura, Yukinori Kazeto, Toshiaki Hirai, Koki Mukai, Ryo Nozu
Summary: In medaka fish, FSH is necessary for follicle development while LH is required for oocyte maturation. However, neither FSH nor LH is essential for spermatogenesis.
Correction
Fisheries
Seiji Hara, Rie Sawamura, Takeshi Kitano
Article
Biochemistry & Molecular Biology
Toshiya Yamaguchi, Takeshi Kitano
Summary: Japanese flounder is a teleost fish with an XX/XY sex determination system and temperature-dependent sex determination. The up-regulation of Amh expression during the sex differentiation period leads to masculinization. In this study, researchers found that the loss of Amhr2 function caused male-to-female sex reversal, confirming the role of Amh/Amhr2 signaling in inhibiting estrogen synthesis for masculinization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Yoko Kato-Unoki, Yuki Takai, Yosuke Nagano, Satoshi Matsunaga, Shintaro Enoki, Takumi Takamura, Sangwan Kim, Masato Kinoshita, Takeshi Kitano, Yohei Shimasaki, Yuji Oshima
MARINE POLLUTION BULLETIN
(2020)