Article
Plant Sciences
Lucia Vieira Hoffmann, Amanda Alves Branquinho, Paulo Augusto Vianna Barroso, Maite F. S. Vaslin
Summary: By developing monoclonal and polyclonal antibodies based on coat protein peptides, an efficient ELISA detection method was established to detect the cotton blue disease virus in cotton and weeds. The study has the potential to guide the development of other diagnostic procedures.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
William W. Spivey, Zachary Williamson, Jacob Seiter, Peter Abrahamian, Hehe Wang, Jeremy Greene, Elizabeth Cieniewicz
Summary: Cotton leafroll dwarf virus (CLRDV) is rapidly spreading in the major cotton-producing states of the southern United States. Variability among CLRDV isolates in South Carolina was found to be low, but should be considered in comparison to other isolates in the United States to understand the overall situation.
Article
Plant Sciences
Surendra R. Edula, Sudeep Bag, Hayley Milner, Manish Kumar, Nelson D. Suassuna, Peng W. Chee, Robert C. Kemerait, Lavesta C. Hand, John L. Snider, Rajagopalbabu Srinivasan, Phillip M. Roberts
Summary: Cotton leafroll dwarf virus (CLRDV) belongs to the genus Polerovirus, family Solemoviridae. It is widely distributed in most cotton-growing regions, especially in North and South America. The virus is efficiently transmitted by the cotton aphid in a circulative and nonpropagative manner. Cotton is the primary host, but the virus has also been detected in weeds in and around commercial cotton fields in Georgia, USA.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Ved Parkash, Divya Bhanu Sharma, John Snider, Sudeep Bag, Phillip Roberts, Afsha Tabassum, Dalton West, Sameer Khanal, Nelson Suassuna, Peng Chee
Summary: CLRDD disease caused by CLRDV poses a new threat to cotton production in the United States, with substantial reductions in productivity observed for individual plants due to limitations in stomatal conductance and photosynthetic activity inflicted by the virus.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Entomology
Jessica B. Mahas, Charles Ray, Adam Kesheimer, Kassie Conner, Alana L. Jacobson
Summary: This study monitored the seasonal population dynamics of eight aphid species in three locations and two habitats in Alabama over two consecutive years using yellow pan traps. The findings provided baseline information on when these species are active in the landscape and when CLRDV spreads in cotton. Although the cotton aphid (A. gossypii) is likely the primary vector, early season spread of CLRDV suggests the possibility of additional aphid vectors.
Article
Virology
Roberto Ramos-Sobrinho, Raphael O. Adegbola, Kathy Lawrence, Drew W. Schrimsher, Thomas Isakeit, Olufemi J. Alabi, Judith K. Brown
Summary: Cotton leafroll dwarf virus (CLRDV) exhibits genomic variability among isolates in the US, with the ORF encoding the P0 silencing suppressor being the most variable gene. Bayesian analysis revealed distinct monophyletic groups of CLRDV isolates from the US and South America.
Article
Agronomy
Nabin P. Sedhain, Sudeep Bag, Kaelyn Morgan, Richard Carter, Patrick Triana, Jared Whitaker, Robert C. Kemerait, Phillip M. Roberts
Summary: In 2020, a comprehensive study on Cotton Leafroll Dwarf Virus (CLRDV) identified various weeds and overwintering cotton as potential sources for the disease, with isolates from the southern United States and Georgia clustering together but differing from isolates from South America and Asia in terms of nucleotide and amino acid sequences. The study highlighted the role of alternative hosts in disease spread and the genetic variations of CLRDV isolates from different regions.
Article
Agronomy
Serina M. Taluja, Brianna L. Cheek, Robert N. Vaughn, Olufemi J. Alabi, John E. Holme, Nisha Jain, David M. Stelly
Summary: We have developed a more efficient, rapid, cost-effective, and simple technique for screening cotton plants for the presence of cotton leafroll dwarf virus (CLRDV) compared to current PCR-based identification methods. This technique utilizes the PACE system, PCR amplification of cDNA, and sequence-specific fluorescent probes to differentiate between infected and uninfected cotton plants. It has the potential for detecting other RNA viruses in various crops by targeting specific genes and conserved housekeeping genes.
Article
Plant Sciences
Bhanu Priya Donda, Sandya R. Kesoju, Kari Arnold, Neil McRoberts, Rayapati A. Naidu
Summary: In this study, the spatial and temporal spread of grapevine leafroll disease (GLD) in three vineyard blocks in eastern Washington State was examined. The results showed a gradual increase in disease incidence over time in all three blocks. The mapping of GLD provided valuable insights for improved area-wide disease management strategies.
Article
Agriculture, Multidisciplinary
Danielle Caroline Manenti, Jackson Araujo, Matheus Hideki Kihara Maeda, Moana Lima Tavares-Esashika, Paulo Hugo Aguiar, Anderson Rotter Meda, Tatsuya Nagata, Eliezer Rodrigues de Souto
Summary: This study overcame the challenges in studying viral diseases in cotton plants by using new experimental techniques and genotyping protocols. The results suggest that atypical-CBD may dominate in cotton fields in Brazil.
ANNALS OF APPLIED BIOLOGY
(2023)
Review
Plant Sciences
W. Allen Miller, Zachary Lozier
Summary: Yellow dwarf viruses are economically important and widespread in cereal crops. They have common biological properties but fall into two unrelated taxa based on their genomes. This article provides an overview of their characteristics, replication mechanisms, and highlights key unanswered questions in research.
ANNUAL REVIEW OF PHYTOPATHOLOGY
(2022)
Article
Multidisciplinary Sciences
Afsha Tabassum, Sudeep Bag, Nelson D. Suassuna, Kassie N. Conner, Peng Chee, Robert C. Kemerait, Phillip Roberts
Summary: The emerging virus Cotton leafroll dwarf virus (CLRDV) in cotton production in the USA, particularly in Georgia and Alabama, has shown genetic diversity in comparison to isolates from South America. Variations in specific amino acid positions, such as 72 and 120, were observed in the isolates from different regions, suggesting potential differences in virulence and resistance-breaking capabilities. Recombination events were also detected in the sequenced isolates, highlighting the need for further research on CLRDV population structure and genetic diversity for effective management strategies in the USA cotton belt.
Article
Plant Sciences
Amanda M. Vondras, Larry Lerno, Melanie Massonnet, Andrea Minio, Adib Rowhani, Dingren Liang, Jadran Garcia, Daniela Quiroz, Rosa Figueroa-Balderas, Deborah A. Golino, Susan E. Ebeler, Maher Al Rwahnih, Dario Cantu
Summary: The responses to Grapevine leafroll-associated virus (GLRaV) infections in Cabernet Franc grapes vary depending on factors such as host genotype, rootstock, environment, and which individual or combination of GLRaVs is present. Different rootstocks and infection conditions led to distinct effects, with conserved responses including gene modulation related to pathogen detection, ABA signaling, phenylpropanoid biosynthesis, and cytoskeleton remodeling. ABA signaling plays a key role in both shared responses to GLRaV infection and differentiating responses in grapevines grafted to different rootstocks.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Entomology
Brianna Heilsnis, Jessica B. Mahas, Kassie Conner, Sudeep Pandey, Wilson Clark, Jenny Koebernick, Rajagopalbabu Srinivasan, Kathleen Martin, Alana L. Jacobson
Summary: Cotton leafroll dwarf virus (CLRDV), a yield-limiting virus transmitted by aphids, was discovered in cotton in the United States in 2017. Aphis gossypii was found to be the only known vector of CLRDV in cotton. In our study, both winged and wingless morphs of A. gossypii were able to acquire the virus within 30 minutes and 24 hours, inoculate the virus within 45 minutes and 15 minutes, and retain the virus for 15 and 23 days, respectively. Myzus persicae and Aphis craccivora did not acquire or transmit CLRDV to cotton.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Agronomy
John W. Mahas, Fredericka B. Hamilton, Phillip M. Roberts, Charles H. Ray, Gary L. Miller, Murray Sharman, Kassie Conner, Sudeep Bag, Eugene K. Blythe, Michael D. Toews, Alana L. Jacobson
Summary: This study is the first to investigate management strategies for cotton leafroll dwarf virus (CLRDV) in the south-eastern U.S. The researchers found that adjusting planting dates and using insecticides did not reduce the incidence of CLRDV, but foliar insecticide applications did reduce aphid population density. Aphis gossypii was the dominant species collected in traps and virus spread mainly occurred during large aphid dispersal events.
Article
Entomology
Seth J. Dorman, Sally V. Taylor, Sean Malone, Phillip M. Roberts, Jeremy K. Greene, Dominic D. Reisig, Ronald H. Smith, Alana L. Jacobson, Francis P. F. Reay-Jones, Silvana Paula-Moraes, Anders S. Huseth
Summary: This study surveyed 120 commercial cotton fields in the southeastern USA to evaluate the variation in tarnished plant bug populations, assess current sampling plans, and examine landscape-scale risk factors. The findings emphasized the importance of field-level scouting and determined the sampling size needed for accurate threshold estimates. The study also identified the positive association between tarnished plant bug densities and the proportion of agriculture and double-crop winter wheat and soybeans, as well as the negative association with contiguous cotton.
Article
Biochemistry & Molecular Biology
Jocelyn G. Millar, Livy Williams, Jacqueline M. Serrano, Sean Halloran, Anna C. Grommes, Anders S. Huseth, Thomas P. Kuhar, Lawrence M. Hanks
Summary: Hexanoic acid, 1-octanol, 1,8-octanediol, octyl hexanoate, 1,8-octanediol monohexanoate, and 1,8-octanediol dihexanoate were identified in the headspace volatiles of a female click beetle. In field trials, adult male beetles were strongly attracted to 1,8-octanediol dihexanoate alone, suggesting it may be the main pheromone compound for this species.
JOURNAL OF CHEMICAL ECOLOGY
(2022)
Article
Entomology
Emily C. Ogburn, Thomas M. Ohmen, Anders S. Huseth, Dominic D. Reisig, George G. Kennedy, James F. Walgenbach
Summary: Brown marmorated stink bug, Halyomorpha halys, was studied in mountain and plains regions, showing univoltine and bivoltine life cycles, respectively. Poor establishment of the bug in plains was attributed to heat stress and oviposition issues. A model was developed to understand the relationship between cumulative degree-days and oviposition and adult eclosion.
JOURNAL OF PEST SCIENCE
(2023)
Article
Agronomy
Benjamin R. Arends, Dominic D. Reisig, Shawnee Gundry, Jeremy K. Greene, George G. Kennedy, Francis P. F. Reay-Jones, Anders S. Huseth
Summary: Despite the widespread occurrence of resistance to Cry toxins in maize, Bt maize continues to provide a measurable reduction in damage. There are negative relationships between kernel injury and ears with larval exit holes in both Bt and non-Bt maize, as well as the proportion of maize in the landscape during the current year.
PEST MANAGEMENT SCIENCE
(2022)
Editorial Material
Plant Sciences
Autumn McLaughlin, Brianna Heilsnis, Jenny Koebernick, Kassie Conner, Alana L. Jacobson
Article
Agronomy
S. J. Patel, K. L. Bowen, J. D. Patel, J. C. Koebernick
Summary: This study used a rapid screening technique to evaluate the resistance of 15 soybean varieties against target spot caused by Corynespora cassiicola. Two resistant varieties were identified, and some varieties showed resistance against different isolates of C. cassiicola. These resistant varieties could be used for further research on the resistance mechanisms of target spot.
Article
Agronomy
John W. Mahas, Todd D. Steury, Anders S. Huseth, Alana L. Jacobson
Summary: An investigation was conducted to characterize the susceptibility of 43 cotton aphid populations to imidacloprid over a 2-year period. The study found variable levels of resistance in the populations, with higher resistance associated with greater cotton production intensity. Rotating modes of action and limiting insecticide use are recommended to delay the evolution of insecticide resistance in cotton aphid populations.
PEST MANAGEMENT SCIENCE
(2023)
Article
Surgery
Christine Park, Tyler Schappe, Sarah Peskoe, Dinushika Mohottige, Norine W. Chan, Nrupen A. Bhavsar, Jane Pendergast, Allan D. Kirk, Lisa M. McElroy
Summary: The choice of deprivation index has an impact on conclusions about deprivation extent and identifying the most deprived communities. This study aimed to determine correlations among commonly used deprivation indices for transplant populations. Different deprivation indices were compared using a retrospective cohort of liver or kidney transplant patients. The choice of deprivation index affects the applicability of research findings on social risk and clinical outcomes. Careful selection of indices based on intended use and variable relevance is necessary for transplant populations.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Agronomy
Cara Mathers, Joshua Heitman, Anders Huseth, Anna Locke, Deanna Osmond, Alexander Woodley
Summary: Understanding the impact of soil management choices on yield stability is crucial for agricultural resiliency in the face of global temperature increases and regional climate changes. Long-term studies are needed to assess the potential benefits of no-till and conservation tillage, as their effects on crop yields vary depending on the crop and location. Findings from a 28-year tillage study in the southeastern U.S. piedmont region suggest that surface residue management may be more important than overall soil health in determining system performance.
FIELD CROPS RESEARCH
(2023)
Article
Entomology
Emma Schoeppner, Jocelyn G. Millar, Thomas P. Kuhar, Helene Doughty, Ronald H. Cherry, Grace Hall, Caitlin G. Knowles, Livy Williams, Anders S. Huseth
Summary: Corn wireworm, Melanotus communis, is an economically important larval pest of root and tuber crops in the United States. Previous studies estimated the field-level abundance of M. communis using grain-based larval baits placed in soil, but this method is labor intensive and may not accurately estimate population size. Recent discovery of the M. communis sex pheromone provides a new method to monitor this pest during the adult stage. This study compared pheromone captures using different trapping methods and tested lure longevity by aging the lures prior to trap deployment. Results showed large variation in M. communis abundance across states, and 1 m elevated pheromone traps caught the most beetles. The age of the lure had a significant effect on trap catch, with younger lures attracting more beetles.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Nandita Banerjee, Mohammad Suhail Khan, M. Swapna, Sonia Yadav, Gopal Ji Tiwari, Satya N. Jena, Jinesh D. Patel, R. Manimekalai, Sanjeev Kumar, S. K. Dattamajuder, Raman Kapur, Jenny C. Koebernick, Ram K. Singh
Summary: A genetic linkage map was developed using a selfed population of sugarcane variety CoS 96268, and a QTL associated with red rot resistance was identified. Key markers involved in red rot resistance were identified through genotype-phenotype network analysis, which was further confirmed by expression analysis. The plant defense-related gene 26S protease regulatory subunit was strongly associated with red rot resistance. These findings have significant implications for screening disease-resistant genotypes and developing red rot-resistant varieties of sugarcane.
Article
Agronomy
Serena J. Thompson, Jenny Koebernick, Liliane S. Silva, Mary K. Mullenix, Courtney Heaton, Russell C. Carrell, Sandra L. Dillard
Summary: The objective of this study was to determine the herbage mass and nutritive value of forage soybean managed under different row spacing and stubble heights. The results showed that the Stonewall cultivar had the highest herbage mass and nutritive value, while Laredo had the lowest herbage mass. A stubble height of 10 cm resulted in increased herbage mass but decreased nutritive value compared to 20 cm. Row spacing did not consistently influence both herbage mass and nutritive values. Therefore, forage soybean should be considered for inclusion in forage-based livestock diets.
Article
Agronomy
Robert Fett, Anne M. Gillen, Quentin D. Read, Sejal Patel, Jenny Koebernick
Summary: The official soybean test weight standard in the United States is 75.7 kg/hL (60 lb/bu), with discounts given when the weight falls below 68.4 kg/hL (54 lb/bu). There is a lack of literature on the comparison of instruments used to measure test weight. This study compared three instruments and found that the Perten Aquamatic 5200 had better accuracy and faster processing speed, while the volumetric instrument had the fastest processing speed but lacked moisture measurement.
AGROSYSTEMS GEOSCIENCES & ENVIRONMENT
(2023)
Article
Entomology
Galen P. Dively, Tom P. Kuhar, Sally V. Taylor, Helene Doughty, Kristian Holmstrom, Daniel O. Gilrein, Brian A. Nault, Joseph Ingerson-Mahar, Anders Huseth, Dominic Reisig, Shelby Fleischer, David Owens, Kelley Tilmon, Francis Reay-Jones, Pat Porter, Jocelyn Smith, Julien Saguez, Jason Wells, Caitlin Congdon, Holly Byker, Bryan Jensen, Chris DiFonzo, William D. Hutchison, Eric Burkness, Robert Wright, Michael Crossley, Heather Darby, Tom Bilbo, Nicholas Seiter, Christian Krupke, Craig Abel, Brad S. Coates, Bradley McManus, Billy Fuller, Jeffrey Bradshaw, Julie A. Peterson, David Buntin, Silvana Paula-Moraes, Katelyn Kesheimer, Whitney Crow, Jeffrey Gore, Fangneng Huang, Dalton C. Ludwick, Amy Raudenbush, Sebastian Jimenez, Yves Carriere, Timothy Elkner, Kelly Hamby
Summary: Genetically modified corn and cotton in the United States are facing a major threat from resistance evolution in Helicoverpa zea populations. A study using Bt sweet corn as a sentinel plant revealed widespread resistance to certain toxins and an increase in ear damage and surviving larvae in the case of Vip3Aa. Better resistance management practices are urgently needed to sustain the efficacy of corn and cotton that produce Vip3Aa.
Article
Entomology
Ariela Haber, Kyle Bekelja, Anders S. Huseth, G. David Buntin, Fred Musser, Jasmin P. Ramirez Bonilla, Sally Taylor, Daniel Wilczek, Ian M. Grettenberger, Donald C. Weber, Thomas Kuhar
Summary: The spotted cucumber beetle, also known as southern corn rootworm, is a polyphagous pest that damages various crops, including cucurbits and corn. The larvae are below-ground pests of corn, peanut, and sweetpotato. Adult damage impacts cucurbits by damaging seedlings and fruits, and they also transmit bacterial wilt. However, the ecology and control methods of this pest are poorly understood.
JOURNAL OF INTEGRATED PEST MANAGEMENT
(2023)
