Article
Plant Sciences
Surendra R. Edula, Sudeep Bag, Hayley Milner, Manish Kumar, Nelson D. Suassuna, Peng W. Chee, Robert C. Kemerait, Lavesta C. Hand, John L. Snider, Rajagopalbabu Srinivasan, Phillip M. Roberts
Summary: Cotton leafroll dwarf virus (CLRDV) belongs to the genus Polerovirus, family Solemoviridae. It is widely distributed in most cotton-growing regions, especially in North and South America. The virus is efficiently transmitted by the cotton aphid in a circulative and nonpropagative manner. Cotton is the primary host, but the virus has also been detected in weeds in and around commercial cotton fields in Georgia, USA.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Agronomy
Nabin P. Sedhain, Sudeep Bag, Kaelyn Morgan, Richard Carter, Patrick Triana, Jared Whitaker, Robert C. Kemerait, Phillip M. Roberts
Summary: In 2020, a comprehensive study on Cotton Leafroll Dwarf Virus (CLRDV) identified various weeds and overwintering cotton as potential sources for the disease, with isolates from the southern United States and Georgia clustering together but differing from isolates from South America and Asia in terms of nucleotide and amino acid sequences. The study highlighted the role of alternative hosts in disease spread and the genetic variations of CLRDV isolates from different regions.
Article
Plant Sciences
William W. Spivey, Zachary Williamson, Jacob Seiter, Peter Abrahamian, Hehe Wang, Jeremy Greene, Elizabeth Cieniewicz
Summary: Cotton leafroll dwarf virus (CLRDV) is rapidly spreading in the major cotton-producing states of the southern United States. Variability among CLRDV isolates in South Carolina was found to be low, but should be considered in comparison to other isolates in the United States to understand the overall situation.
Article
Plant Sciences
Ved Parkash, Divya Bhanu Sharma, John Snider, Sudeep Bag, Phillip Roberts, Afsha Tabassum, Dalton West, Sameer Khanal, Nelson Suassuna, Peng Chee
Summary: CLRDD disease caused by CLRDV poses a new threat to cotton production in the United States, with substantial reductions in productivity observed for individual plants due to limitations in stomatal conductance and photosynthetic activity inflicted by the virus.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Entomology
Jessica B. Mahas, Charles Ray, Adam Kesheimer, Kassie Conner, Alana L. Jacobson
Summary: This study monitored the seasonal population dynamics of eight aphid species in three locations and two habitats in Alabama over two consecutive years using yellow pan traps. The findings provided baseline information on when these species are active in the landscape and when CLRDV spreads in cotton. Although the cotton aphid (A. gossypii) is likely the primary vector, early season spread of CLRDV suggests the possibility of additional aphid vectors.
Article
Virology
Roberto Ramos-Sobrinho, Raphael O. Adegbola, Kathy Lawrence, Drew W. Schrimsher, Thomas Isakeit, Olufemi J. Alabi, Judith K. Brown
Summary: Cotton leafroll dwarf virus (CLRDV) exhibits genomic variability among isolates in the US, with the ORF encoding the P0 silencing suppressor being the most variable gene. Bayesian analysis revealed distinct monophyletic groups of CLRDV isolates from the US and South America.
Article
Agriculture, Multidisciplinary
Danielle Caroline Manenti, Jackson Araujo, Matheus Hideki Kihara Maeda, Moana Lima Tavares-Esashika, Paulo Hugo Aguiar, Anderson Rotter Meda, Tatsuya Nagata, Eliezer Rodrigues de Souto
Summary: This study overcame the challenges in studying viral diseases in cotton plants by using new experimental techniques and genotyping protocols. The results suggest that atypical-CBD may dominate in cotton fields in Brazil.
ANNALS OF APPLIED BIOLOGY
(2023)
Article
Plant Sciences
Lucia Vieira Hoffmann, Amanda Alves Branquinho, Paulo Augusto Vianna Barroso, Maite F. S. Vaslin
Summary: By developing monoclonal and polyclonal antibodies based on coat protein peptides, an efficient ELISA detection method was established to detect the cotton blue disease virus in cotton and weeds. The study has the potential to guide the development of other diagnostic procedures.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Yaqin Wang, Hui Liu, Zhanqi Wang, Yushuang Guo, Tao Hu, Xueping Zhou
Summary: This study found that the P25 and P37 proteins of FLRaV play important roles in overcoming host RNA silencing and aggravating the symptoms and titer of virus infection, providing key insights into the molecular mechanisms underlying FLRaV infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Licheng Wang, Wenbao Chen, Huan Ma, Jingyuan Li, Xingan Hao, Yunfeng Wu
Summary: The phytoplasma encoded RSS SWP16 inhibits RNA silencing in plants, leading to viral infection and providing insight into pathogen-plant interaction mechanisms.
Article
Microbiology
Connor Ferguson, Akhtar Ali
Summary: This study presents the complete genome sequence of a Cotton leafroll dwarf virus (CLRDV) isolate, named EC4, collected during the 2021 cotton growing season in Oklahoma, United States.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Agronomy
Serina M. Taluja, Brianna L. Cheek, Robert N. Vaughn, Olufemi J. Alabi, John E. Holme, Nisha Jain, David M. Stelly
Summary: We have developed a more efficient, rapid, cost-effective, and simple technique for screening cotton plants for the presence of cotton leafroll dwarf virus (CLRDV) compared to current PCR-based identification methods. This technique utilizes the PACE system, PCR amplification of cDNA, and sequence-specific fluorescent probes to differentiate between infected and uninfected cotton plants. It has the potential for detecting other RNA viruses in various crops by targeting specific genes and conserved housekeeping genes.
Article
Virology
Dan Wang, Shuai Fu, Hongyue Wu, Mengji Cao, Li Liu, Xueping Zhou, Jianxiang Wu
Summary: Bunyaviruses can cause diseases in various organisms, including vertebrates, arthropods, and plants. In this study, a bunya-like virus named RDaBV was identified in rice plants showing dwarfing symptoms using high-throughput RNA-seq. The genome of RDaBV consists of three segments (L, M, and S) and encodes various proteins including an RdRp, NS, N, and NSs. Phylogenetic analysis revealed that RDaBV is closely related to viruses in the family Discoviridae. Functional experiments indicated that RDaBV NSs is a viral movement protein in plants, while RDaBV NS functions as an RNA silencing suppressor. This study demonstrates that RDaBV is a novel bunya-like virus infecting rice.
Article
Entomology
Brianna Heilsnis, Jessica B. Mahas, Kassie Conner, Sudeep Pandey, Wilson Clark, Jenny Koebernick, Rajagopalbabu Srinivasan, Kathleen Martin, Alana L. Jacobson
Summary: Cotton leafroll dwarf virus (CLRDV), a yield-limiting virus transmitted by aphids, was discovered in cotton in the United States in 2017. Aphis gossypii was found to be the only known vector of CLRDV in cotton. In our study, both winged and wingless morphs of A. gossypii were able to acquire the virus within 30 minutes and 24 hours, inoculate the virus within 45 minutes and 15 minutes, and retain the virus for 15 and 23 days, respectively. Myzus persicae and Aphis craccivora did not acquire or transmit CLRDV to cotton.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Agronomy
John W. Mahas, Fredericka B. Hamilton, Phillip M. Roberts, Charles H. Ray, Gary L. Miller, Murray Sharman, Kassie Conner, Sudeep Bag, Eugene K. Blythe, Michael D. Toews, Alana L. Jacobson
Summary: This study is the first to investigate management strategies for cotton leafroll dwarf virus (CLRDV) in the south-eastern U.S. The researchers found that adjusting planting dates and using insecticides did not reduce the incidence of CLRDV, but foliar insecticide applications did reduce aphid population density. Aphis gossypii was the dominant species collected in traps and virus spread mainly occurred during large aphid dispersal events.
Editorial Material
Plant Sciences
Saritha Raman Kavalappara, Hayley Milner, Alton Sparks, Cecilia McGregor, William M. Wintermantel, Sudeep Bag
Editorial Material
Plant Sciences
A. Abdul Kader Jailani, Fanny Iriarte, Bob Hochmuth, Sylvia M. Willis, Mark Warren, Kishore Dey, Maria Velez-Climent, John McVay, Sudeep Bag, Mathews L. Paret
Article
Horticulture
Alexander Luckew, Geoffrey Meru, Ya-Ying Wang, Rodrick Mwatuwa, Mathews Paret, Renato Carvalho, Melanie Kalischuk, Andre Luiz Biscaia, Ribeiro da Silva, Joara Candian, Bhabesh Dutta, Rajagopalbabu Srinivasan, Saritha Raman Kavalappara, R. R. D. Naga Charan Konakalla, Sudeep Bag, Cecilia McGregor
Summary: Summer squash is a major vegetable crop in Georgia and Florida that is susceptible to whitefly-transmitted viruses. This study evaluated the resistance or tolerance of different squash germplasms to whiteflies and the viruses they transmit. Potential resistant sources were identified, which can help mitigate yield losses.
Editorial Material
Plant Sciences
Saritha Raman Kavalappara, Hayley Milner, David G. Riley, Sudeep Bag
Editorial Material
Plant Sciences
Ismaila Adeyemi Adeleke, Saritha Raman Kavalappara, Ty Tolerance, John Bennett, Cecilia McGregor, Rajagopalbabu Srinivasan, Sudeep Bag
Article
Virology
Saritha R. Kavalappara, David G. Riley, Paulo S. G. Cremonez, Jermaine D. Perier, Sudeep Bag
Summary: Cucurbit chlorotic yellows virus (CCYV), a virus transmitted by whiteflies, was detected in wild radish in the southeastern USA. Wild radish was found to be a good host for CCYV, and whiteflies were able to acquire and transmit the virus to cucurbit hosts. These findings suggest that wild radish may serve as a reservoir host for CCYV in the USA and other regions.
Article
Agronomy
John W. Mahas, Fredericka B. Hamilton, Phillip M. Roberts, Charles H. Ray, Gary L. Miller, Murray Sharman, Kassie Conner, Sudeep Bag, Eugene K. Blythe, Michael D. Toews, Alana L. Jacobson
Summary: This study is the first to investigate management strategies for cotton leafroll dwarf virus (CLRDV) in the south-eastern U.S. The researchers found that adjusting planting dates and using insecticides did not reduce the incidence of CLRDV, but foliar insecticide applications did reduce aphid population density. Aphis gossypii was the dominant species collected in traps and virus spread mainly occurred during large aphid dispersal events.
Article
Virology
Ismaila Adeyemi Adeleke, Saritha Raman Kavalappara, Cecilia McGregor, Rajagopalbabu Srinivasan, Sudeep Bag
Summary: Cucurbits in Southeastern USA have experienced a drastic decline in production over the years due to the effect of economically important viruses, mainly those transmitted by the sweet potato whitefly (Bemisia tabaci Gennadius). In this study, surveys were conducted to assess the incidence and distribution of viruses infecting cantaloupe and watermelon in Georgia. The findings revealed the presence of several new RNA viruses in Georgia, as well as the detection of known viruses in melons.
Article
Virology
Sudeep Pandey, Sudeep Bag, Phillip Roberts, Kassie Conner, Kipling S. Balkcom, Andrew J. Price, Alana L. Jacobson, Rajagopalbabu Srinivasan
Summary: This study found that Cotton leafroll dwarf virus (CLRDV) can be transmitted through other plant hosts, potentially impacting the epidemic of CLRDV in the southeastern United States.
Article
Plant Sciences
Surendra R. Edula, Sudeep Bag, Hayley Milner, Manish Kumar, Nelson D. Suassuna, Peng W. Chee, Robert C. Kemerait, Lavesta C. Hand, John L. Snider, Rajagopalbabu Srinivasan, Phillip M. Roberts
Summary: Cotton leafroll dwarf virus (CLRDV) belongs to the genus Polerovirus, family Solemoviridae. It is widely distributed in most cotton-growing regions, especially in North and South America. The virus is efficiently transmitted by the cotton aphid in a circulative and nonpropagative manner. Cotton is the primary host, but the virus has also been detected in weeds in and around commercial cotton fields in Georgia, USA.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Virology
Saritha Raman Kavalappara, Sudeep Bag, Alex Luckew, Cecilia E. McGregor
Summary: RNA silencing is a crucial mechanism in plants' antiviral immunity system. Small RNAs guide Argonaut proteins to target viral RNA or DNA, preventing virus accumulation. Comparison of small RNA profiles between Cucurbita pepo line PI 420328 and Gold Star revealed that PI 420328 had lower CYSDV symptom severity, lower virus titers, and fewer CYSDV-derived sRNAs. However, PI 420328 exhibited elevated levels of 21- and 22-nucleotide size class vsRNAs, indicating stronger and more efficient RNA silencing. The distribution of vsRNA hotspots along the CYSDV genome was similar in both PI 420328 and Gold Star, but certain regions were targeted more frequently in PI 420328.
Article
Microbiology
Yi-Ju Chen, Sudeep Pandey, Michael Catto, Soraya Leal-Bertioli, Mark R. Abney, Sudeep Bag, Mark Hopkins, Albert Culbreath, Rajagopalbabu Srinivasan
Summary: This study demonstrates the resistance of wild diploid Arachis species and peanut-compatible-induced allotetraploids against TSWV and thrips through thrips-mediated transmission assays and feeding assays. Among the evaluated genotypes, certain diploids and tetraploid hybrids exhibited reduced TSWV infection, virus accumulation, and thrips feeding damage.
Review
Virology
Ragunathan Devendran, Saritha Raman Kavalappara, Alvin M. Simmons, Sudeep Bag
Summary: This review discusses the negative impact of whitefly-transmitted viruses on Cucurbits, focusing on three major groups of WTVs. The article provides an overview of the biology, epidemiology, and advancements in detecting and managing these viruses.
Article
Agronomy
Nelson Dias Suassuna, Camilo de Lelis Morello, Fabiano Jose Perina, Joao Luis da Silva Filho, Murilo Barros Pedrosa, Fernanda Oliveira da Cunha Magalhaes, Valdinei Sofiatti, Fernando Mendes Lamas, Luiz Gonzaga Chitarra, Francisco J. Correia Farias, Wellington Costa Rodrigues do O
Summary: BRS 500 B2RF is a high-yielding cotton cultivar with transgenic resistance to lepidopteran pests and tolerance to the herbicide glyphosate. It also has resistance to Ramularia leaf spot and partial resistance to root-knot nematode, making it an important contribution to sustainable cotton production in Brazil.
CROP BREEDING AND APPLIED BIOTECHNOLOGY
(2021)
