Generation of Transgenic Cloned Buffalo Embryos Harboring the EGFP Gene in the Y Chromosome Using CRISPR/Cas9-Mediated Targeted Integration

CRISPR/Cas9-mediated knockin of human factor IX into swine factor IX locus effectively alleviates bleeding in hemophilia B pigs

(2020) Jiahuan Chen et al.   HAEMATOLOGICA

Precise editing of myostatin signal peptide by CRISPR/Cas9 increases the muscle mass of Liang Guang Small Spotted pigs

(2020) Ruiqiang Li et al.   TRANSGENIC RESEARCH

A 5-bp mutation within MSTN/GDF8 gene was significantly associated with growth traits in Inner Mongolia White Cashmere goats

(2020) Yi Bi et al.   ANIMAL BIOTECHNOLOGY

Identification of the Sex of Pre-implantation Mouse Embryos Using a Marked Y Chromosome and CRISPR/Cas9

(2019) Xiuling Zhao et al.   Scientific Reports

Establishment, Growth, Proliferation, and Gene Expression of Buffalo (Bubalus bubalis) Transgenic Fetal Fibroblasts Containing Human Insulin Gene, and Production of Embryos by Handmade Cloning Using These Cells

(2018) Parul Mehta et al.   Cellular Reprogramming

Genetically engineered pigs as models for human disease

(2018) Carolin Perleberg et al.   Disease Models & Mechanisms

p53 inhibits CRISPR–Cas9 engineering in human pluripotent stem cells

(2018) Robert J. Ihry et al.   NATURE MEDICINE

CRISPR: Stressed about p53?

(2018) Miguel Foronda et al.   TRENDS IN MOLECULAR MEDICINE

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

(2018) Xuan Yao et al.   Jove-Journal of Visualized Experiments

Somatic Cell-Induced Hyperacetylation, But Not Hypomethylation, Positively and Reversibly Affects the Efficiency of In Vitro Cloned Blastocyst Production in Cattle

(2018) Farnoosh Jafarpour et al.   Cellular Reprogramming

Effects of Donor Fibroblast Cell Type and Transferred Cloned Embryo Number on the Efficiency of Pig Cloning

(2018) Zicong Li et al.   Cellular Reprogramming

A transgenic minipig model of Huntington's disease shows early signs of behavioral and molecular pathologies

(2018) Georgina Askeland et al.   Disease Models & Mechanisms

CRISPR/Cas9-Mediated Generation of Guangxi Bama Minipigs Harboring Three Mutations in α-Synuclein Causing Parkinson’s Disease

(2018) Xiang-Xing Zhu et al.   Scientific Reports

Optimization of a CRISPR/Cas9-mediated Knock-in Strategy at the Porcine Rosa26 Locus in Porcine Foetal Fibroblasts

(2017) Zicong Xie et al.   Scientific Reports

Expression of the Alzheimer’s Disease Mutations AβPP695sw and PSEN1M146I in Double-Transgenic Göttingen Minipigs

(2016) Jannik E. Jakobsen et al.   JOURNAL OF ALZHEIMERS DISEASE

Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos

(2016) Yan Jun HUAN et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions

(2016) D. M. Munoz et al.   Cancer Discovery

Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting

(2016) A. J. Aguirre et al.   Cancer Discovery

Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair

(2016) Tony Gutschner et al.   Cell Reports

Successful cloning of an adult breeding boar from the novel Chinese Guike No. 1 swine specialized strain

(2016) Jun-yu Nie et al.   3 Biotech

Successful cloning of an adult breeding boar from the novel Chinese Guike No. 1 swine specialized strain

(2016) Jun-yu Nie et al.   3 Biotech

A novel porcine model of ataxia telangiectasia reproduces neurological features and motor deficits of human disease

(2015) Rosanna Beraldi et al.   HUMAN MOLECULAR GENETICS

In vitro production of cloned and transgenically cloned embryos from Guangxi Huanjiang Xiang pig

(2015) Xiangxing Zhu et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

Engineering large animal models of human disease

(2015) C Bruce A Whitelaw et al.   JOURNAL OF PATHOLOGY

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

(2015) Takeshi Maruyama et al.   NATURE BIOTECHNOLOGY

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells

(2015) Van Trung Chu et al.   NATURE BIOTECHNOLOGY

MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems

(2015) Tetsushi Sakuma et al.   Nature Protocols

Tailored Pig Models for Preclinical Efficacy and Safety Testing of Targeted Therapies

(2015) Nikolai Klymiuk et al.   TOXICOLOGIC PATHOLOGY

Pharmacological inhibition of DNA-PK stimulates Cas9-mediated genome editing

(2015) Francis Robert et al.   Genome Medicine

Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

(2015) Yu Hisano et al.   Scientific Reports

Reporter mouse strain provides a novel look at angiotensin type-2 receptor distribution in the central nervous system

(2014) Annette D. de Kloet et al.   Brain Structure & Function

In vitro development of porcine transgenic nuclear-transferred embryos derived from newborn Guangxi Bama mini-pig kidney fibroblasts

(2014) Hongbo Liu et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

Epigenetic Modification Agents Improve Genomic Methylation Reprogramming in Porcine Cloned Embryos

(2014) Yan Jun HUAN et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

(2014) Shota Nakade et al.   Nature Communications

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set

(2013) Mayuko Kurome et al.   BMC BIOTECHNOLOGY

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Genetically Modified Pig Models for Human Diseases

(2013) Nana Fan et al.   Journal of Genetics and Genomics

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Rapid non-invasive genotyping of reporter transgenic mammals

(2012) Wiebke Garrels et al.   BIOTECHNIQUES

Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

(2012) Anne Richter et al.   BMC BIOTECHNOLOGY

Incomplete methylation reprogramming in SCNT embryos

(2012) Julian R Peat et al.   NATURE GENETICS

Recent advancements in cloning by somatic cell nuclear transfer

(2012) A. Ogura et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Reprogramming mammalian somatic cells

(2012) N. Rodriguez-Osorio et al.   THERIOGENOLOGY

Methylation characteristics and developmental potential of Guangxi Bama minipig (Sus scrofa domestica) cloned embryos from donor cells treated with trichostatin A and 5-aza-2′-deoxycytidine

(2012) Shu-Fang Ning et al.   ZYGOTE

Links between mutant p53 and genomic instability

(2011) Walter Hanel et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Effects of Downregulating DNA Methyltransferase 1 Transcript by RNA Interference on DNA Methylation Status of the Satellite I Region and In Vitro Development of Bovine Somatic Cell Nuclear Transfer Embryos

(2011) Ken-ichi YAMANAKA et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Modelling of Parkinson's disease in mice

(2011) Marie-Françoise Chesselet et al.   LANCET NEUROLOGY

Unfaithful Maintenance of Methylation Imprints Due to Loss of Maternal Nuclear Dnmt1 during Somatic Cell Nuclear Transfer

(2011) Yanchang Wei et al.   PLoS One

Antileukemic activity of the HSP70 inhibitor pifithrin-μ in acute leukemia

(2011) M Kaiser et al.   Blood Cancer Journal

Effect of epigenetic regulation during swine embryogenesis and on cloning by nuclear transfer

(2010) Jianguo Zhao et al.   CELL AND TISSUE RESEARCH

Somatic cell nuclear transfer efficiency: How can it be improved through nuclear remodeling and reprogramming?

(2010) Kristin M. Whitworth et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Modes of p53 Regulation

(2009) Jan-Philipp Kruse et al.   CELL

Development of a real-time PCR method for rapid sexing of human preimplantation embryos

(2009) CD Martinhago et al.   REPRODUCTIVE BIOMEDICINE ONLINE

Genome-Wide Analysis of Abnormal H3K9 Acetylation in Cloned Mice

(2008) Takahiro Suzuki et al.   PLoS One

Increased pre-implantation development of cloned bovine embryos treated with 5-aza-2′-deoxycytidine and trichostatin A

(2008) X. Ding et al.   THERIOGENOLOGY

Transgene Expression of Green Fluorescent Protein and Germ Line Transmission in Cloned Pigs Derived from In Vitro Transfected Adult Fibroblasts

(2008) Dario Brunetti et al.   Cloning Stem Cells