MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems

Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids

(2015) Tetsushi Sakuma et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Error-Prone Repair of DNA Double-Strand Breaks

(2015) Kasey Rodgers et al.   JOURNAL OF CELLULAR PHYSIOLOGY

53BP1 promotes microhomology-mediated end-joining in G1-phase cells

(2015) Xiahui Xiong et al.   NUCLEIC ACIDS RESEARCH

Intron-based genomic editing: a highly efficient method for generating knockin zebrafish

(2015) Jia Li et al.   Oncotarget

Comparison of T7E1 and Surveyor Mismatch Cleavage Assays to Detect Mutations Triggered by Engineered Nucleases

(2015) Léna Vouillot et al.   G3-Genes Genomes Genetics

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9

(2015) Hongmei Lisa Li et al.   Stem Cell Reports

A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells

(2015) Zengrong Zhu et al.   Stem Cell Reports

Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish

(2015) Yu Hisano et al.   Scientific Reports

Nuclease-mediated genome editing: At the front-line of functional genomics technology

(2014) Tetsushi Sakuma et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Screening Methods to Identify TALEN-Mediated Knockout Mice

(2014) Yoshiko Nakagawa et al.   EXPERIMENTAL ANIMALS

Microhomology-based choice of Cas9 nuclease target sites

(2014) Sangsu Bae et al.   NATURE METHODS

A guide to genome engineering with programmable nucleases

(2014) Hyongbum Kim et al.   NATURE REVIEWS GENETICS

RPA puts the brakes on MMEJ

(2014) Mitch McVey   NATURE STRUCTURAL & MOLECULAR BIOLOGY

CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in Trypanosoma cruzi

(2014) D. Peng et al.   mBio

Efficient genome engineering by targeted homologous recombination in mouse embryos using transcription activator-like effector nucleases

(2014) Daniel Sommer et al.   Nature Communications

Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

(2014) Shota Nakade et al.   Nature Communications

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

(2014) Tetsushi Sakuma et al.   Scientific Reports

Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems

(2014) Akihiro Yasue et al.   Scientific Reports

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Efficient TALEN construction and evaluation methods for human cell and animal applications

(2013) Tetsushi Sakuma et al.   GENES TO CELLS

Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays

(2013) Satoshi Ota et al.   GENES TO CELLS

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

(2013) Scott J. Gratz et al.   GENETICS

Highly efficient CRISPR/Cas9-mediated knock-in in zebrafish by homology-independent DNA repair

(2013) T. O. Auer et al.   GENOME RESEARCH

Biochemical DSB-repair model for mammalian cells in G1 and early S phases of the cell cycle

(2013) Reza Taleei et al.   MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

(2013) Nathan C Shaner et al.   NATURE METHODS

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes

(2013) Tomoji Mashimo et al.   Scientific Reports

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

(2013) Daisuke Mashiko et al.   Scientific Reports

Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity

(2013) Tetsushi Sakuma et al.   Scientific Reports

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

(2013) K.-i. T. Suzuki et al.   Biology Open

In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration

(2012) Sandra Cristea et al.   BIOTECHNOLOGY AND BIOENGINEERING

Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joining

(2012) M. Maresca et al.   GENOME RESEARCH

In vivo genome editing using a high-efficiency TALEN system

(2012) Victoria M. Bedell et al.   NATURE

Modeling disease mutations by gene targeting in one-cell mouse embryos

(2012) M. Meyer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome

(2012) Timothy J. Dahlem et al.   PLoS Genetics

Targeted chromosomal duplications and inversions in the human genome using zinc finger nucleases

(2011) H. J. Lee et al.   GENOME RESEARCH

Genetic engineering of human pluripotent cells using TALE nucleases

(2011) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

Tracking genome engineering outcome at individual DNA breakpoints

(2011) Michael T Certo et al.   NATURE METHODS

Chemically defined conditions for human iPSC derivation and culture

(2011) Guokai Chen et al.   NATURE METHODS

A TALE nuclease architecture for efficient genome editing

(2010) Jeffrey C Miller et al.   NATURE BIOTECHNOLOGY

Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology

(2010) Salvatore J. Orlando et al.   NUCLEIC ACIDS RESEARCH

Targeted chromosomal deletions in human cells using zinc finger nucleases

(2009) H. J. Lee et al.   GENOME RESEARCH

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

(2009) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY