Review
Biotechnology & Applied Microbiology
Meng Liu, Xin Liu, Yuting Su, Shijie Li, Yuan Chen, Anqi Liu, Jing Guo, Kun Xuan, Xinyu Qiu
Summary: MSC-derived extracellular vesicles (MSC-EVs) have been identified as a promising therapy for tissue repair and regeneration, particularly in oral and craniomaxillofacial tissues. These diseases, induced by various factors, can be prevented and treated through tissue repair and regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Engineering, Biomedical
Vishnu Priya Murali, Christina A. Holmes
Summary: This review summarizes various biomaterial-based extracellular vesicle (EV) delivery systems used in preclinical tissue regeneration studies and other therapeutic applications. EV-based therapies have shown success in preclinical models for biomedical applications, but the challenge lies in prolonging EV retention at target sites for improved therapeutic efficacy through biomaterial-based systems.
ACTA BIOMATERIALIA
(2021)
Review
Engineering, Biomedical
Tyler J. DiStefano, Keti Vaso, George Danias, Henry N. Chionuma, Jennifer R. Weiser, James C. Iatridis
Summary: Research has shown that stem cell-derived EVs can slow down the progression of IVDD at the molecular, cellular, and organ levels. The pathophysiological and differentiation state of the EV parent cell affects downstream treatment responses and therapeutic outcomes. These findings support the use of EVs as a promising cell-free strategy for treating IVDD and enhancing endogenous repair.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Astgik Petrosyan, Paulo. N. N. Martins, Kim Solez, Basak. E. E. Uygun, Vijay. S. S. Gorantla, Giuseppe Orlando
Summary: Insights into the use of cellular therapeutics, extracellular vesicles (EVs), and tissue engineering strategies for regenerative medicine applications are continually emerging. These approaches have shown strong potential in modulating inflammatory immune responses, promoting regeneration in diseased organs, and treating inflammatory diseases like COVID-19. The field of stem cell therapies, in particular, has seen significant advancements in understanding stem cell heterogeneity and dosage specificity, leading to expanded clinical applications. Standardizing manufacturing protocols for tissue-engineered grafts is a crucial step towards whole organ engineering. Despite challenges, the prospects for personalized regenerative and transplant medicine are promising.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Hugo Abreu, Elena Canciani, Davide Raineri, Giuseppe Cappellano, Lia Rimondini, Annalisa Chiocchetti
Summary: Tissue regeneration is a hot topic in health sciences. Studying the relationship between mesenchymal stem/stromal cells (MSCs), extracellular vesicles (EVs), and biomaterials is important for the treatment of musculoskeletal diseases.
Article
Biology
W. Benton Swanson, Yuji Mishina
Summary: Mesenchymal stem cells (MSCs) play a critical role in regenerative medicine, and cell-derived extracellular vesicles have potential as a regenerative tool to enhance the synergy between MSCs and biomaterials.
Review
Medicine, Research & Experimental
Min Li, Fang Fang, Meng Sun, Yinfeng Zhang, Min Hu, Jinfeng Zhang
Summary: Extracellular vesicles (EVs) have emerged as highly attractive nanotherapeutics for regenerative medicine, due to their bioactivity and involvement in various physiological and pathological processes. This systematic review provides insights into the latest progress of EVs in tissue repair and regeneration, including their biogenesis, function, isolation technology, therapeutic mechanisms, and engineering strategies. The applications of EVs on different organs such as the brain, heart, bone and cartilage, liver and kidney, and skin are discussed, along with a brief highlight of current clinical trials. Future challenges and perspectives of EV-based nanotherapeutics in biomedicine are also explored.
Review
Biotechnology & Applied Microbiology
Michela Pozzobon, Stefania D'Agostino, Maria G. G. Roubelakis, Anna Cargnoni, Roberto Gramignoli, Susanne Wolbank, Florelle Gindraux, Sveva Bollini, Halima Kerdjoudj, Mathilde Fenelon, Roberta Di Pietro, Mariangela Basile, Veronika Borutinskaite, Roberta Piva, Andreina Schoeberlein, Guenther Eissner, Bernd Giebel, Peter Ponsaerts
Summary: Perinatal tissues contain various types of stem cells that have the potential for regenerative therapies. This review summarizes the therapeutic approaches using perinatal derivatives (PnD) products and discusses the importance of validating their therapeutic efficacy and the concept of PnD application in tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
W. Benton Swanson, Maiko Omi, Seth M. Woodbury, Lindsey M. Douglas, Miranda Eberle, Peter X. Ma, Nan E. Hatch, Yuji Mishina
Summary: This study investigated the impact of scaffold pore curvature on the differentiation trajectory of mesenchymal stem cells (MSCs), demonstrating that the curvature of scaffold pores can influence cell aggregation and differentiation by altering the cytoskeleton, ultimately guiding cell fate specification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Veterinary Sciences
Hussein M. M. El-Husseiny, Eman A. A. Mady, Mahmoud A. Y. Helal, Ryou Tanaka
Summary: The use of stem cells in veterinary practice is rapidly evolving, with significant research efforts focused on both experimental animals and clinical cases. Stem cells, particularly mesenchymal stem cells (MSCs), are promising for therapeutic applications due to their immunomodulatory capabilities and ease of isolation. However, challenges remain in their clinical implementation for treating various animal diseases, driving further advancements in stem cell therapies.
VETERINARY SCIENCES
(2022)
Review
Cell & Tissue Engineering
Vera Citro, Marta Clerici, Aldo R. Boccaccini, Giovanna Della Porta, Nicola Maffulli, Nicholas R. Forsyth
Summary: Tendons are dense connective tissues that enable motion and maintain posture by transmitting muscle contraction forces to the skeleton. Tendon injuries, known as tendinopathies, are becoming more common due to aging populations and increased participation in sports/leisure activities. Tendon tissue engineering has emerged as a potential approach to drive tendon regeneration and healing.
JOURNAL OF TISSUE ENGINEERING
(2023)
Review
Pharmacology & Pharmacy
Rui Alvites, Mariana Branquinho, Ana C. Sousa, Bruna Lopes, Patricia Sousa, Ana Colette Mauricio
Summary: Mesenchymal Stem/Stromal Cells (MSCs) have high clinical value and have been extensively studied. Their immunomodulatory influence occurs through both direct and paracrine routes, but the mechanisms are not fully understood. This review summarizes the immunoregulatory capacity of MSCs and their secretion products, with a focus on migration, homing, direct cell action, and paracrine activity, and explores related techniques.
Review
Pharmacology & Pharmacy
Marta Bryk, Elzbieta Karnas, Jakub Mlost, Ewa Zuba-Surma, Katarzyna Starowicz
Summary: MSCs, as multipotent progenitor cells derived from mesoderm, have been extensively studied for their regenerative properties, including the release of EVs which play a role in their functions. They regulate various processes in target cells and show potential applications in pain treatment and other diseases.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Review
Cell Biology
Yu Lu, Yu Yang, Shiyu Liu, Shaohua Ge
Summary: This article summarizes the application of extracellular vesicles (EVs) in tissue regeneration, focusing on EV engineering and loading system construction methods.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Cristina Antich, Gema Jimenez, Juan de Vicente, Elena Lopez-Ruiz, Carlos Chocarro-Wrona, Carmen Grinan-Lison, Esmeralda Carrillo, Elvira Montanez, Juan A. Marchal
Summary: The development of a novel biomimetic hydrogel based on decellularized extracellular matrix derived from mesenchymal stem cells for cartilage tissue engineering has shown excellent biocompatibility and the ability to induce chondrogenesis without supplemental factors. This hydrogel also demonstrated the potential to form hyaline cartilage-like tissue after in vivo implantation, highlighting its promise for cartilage repair and regeneration applications.
ADVANCED HEALTHCARE MATERIALS
(2021)
Editorial Material
Pharmacology & Pharmacy
Lorraine O'Driscoll, Willem Stoorvogel, Clotilde Thery, Edit Buzas, Irina Nazarenko, Pia Siljander, Maria Yanez-Mo, Stefano Fais, Bernd Giebel, Marjo Yliperttula
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2017)
Review
Cell Biology
Mariadelva Catalano, Lorraine O'Driscoll
JOURNAL OF EXTRACELLULAR VESICLES
(2020)
Editorial Material
Pharmacology & Pharmacy
Lorraine O'Driscoll
DRUG DISCOVERY TODAY
(2020)
Review
Pharmacology & Pharmacy
Roisin Daly, Lorraine O'Driscoll
Summary: Extracellular vesicles in the bloodstream could serve as important sources of biomarkers for breast cancer, but the current methods for isolating pure EVs are relatively complex. Research is underway to identify simpler and more selective EV extraction methods, with hope of finding effective biomarkers.
DRUG DISCOVERY TODAY
(2021)
Article
Oncology
Sarai Martinez-Pacheco, Lorraine O'Driscoll
Summary: The characteristics of extracellular vesicles (EVs) can vary depending on the cell line and method of separation used, indicating the importance of considering multiple EV sources when selecting a separation technique for research or clinical purposes.
Article
Oncology
Sarai Martinez-Pacheco, Lorraine O'Driscoll
Summary: The majority of cancer researchers currently rely on two-dimensional (2D) cell culture models due to lack of experience and costs, but they express interest in transitioning to three-dimensional (3D) models, which are considered more clinically relevant. There is an urgent need to standardize reliable, robust, and cost-effective methods for establishing 3D cell culture models in cancer research.
Article
Nutrition & Dietetics
Ian A. J. Darragh, Lorraine O'Driscoll, Brendan Egan
Summary: This study examined within-subject variability in the circulating metabolome under controlled conditions and investigated the association between divergent exercise training backgrounds and alterations in the circulating metabolome in resting samples. The results showed that the reliability of resting plasma metabolite concentrations varied at the level of individual metabolites, and a history of exercise training was associated with alterations in the abundance of certain metabolites.
INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM
(2023)
Article
Pharmacology & Pharmacy
Anindya Mukhopadhya, Dimitrios Tsiapalis, Niamh McNamee, Brian Talbot, Lorraine O'Driscoll
Summary: This study compared the suitability of MSC EVs and milk EVs as drug delivery vehicles. Milk EVs had significantly higher yields compared to MSC EVs. Electroporation was found to be the most effective method for loading the anti-cancer drug Dox into EVs. Additionally, sonication was observed to have detrimental effects on EVs.
Article
Neurosciences
Ian A. J. Darragh, Niamh McNamee, Roisin Daly, Sarai Martinez Pacheco, Lorraine O'Driscoll, Brendan Egan
Summary: This study investigated whether individuals with different exercise training histories had varied abundances of small extracellular vesicles (EVs) in resting samples. The results suggest that the abundance and metabolomic profile of small EVs derived from men with divergent exercise training histories are similar to those in resting blood samples.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Cell & Tissue Engineering
Dimitrios Tsiapalis, Achilleas Floudas, Tobias Tertel, Verena Boerger, Bernd Giebel, Douglas J. Veale, Ursula Fearon, Lorraine O'Driscoll
Summary: This study investigated the effect of human mesenchymal/stromal stem cells and their extracellular vesicles (EVs) on CD4+ T cells from rheumatoid arthritis (RA) patients. The researchers found that EVs from IFN-beta-primed MSCs had a stronger immune-regulatory effect on RA CD4+ T cells compared to MSCs or EVs from naive MSCs. In addition, EVs from IFN-beta-primed MSCs effectively inhibited RA FLS migration and downregulated RA FLS surface markers. These findings suggest that EVs from IFN-beta-primed MSCs could be a potential therapy for RA.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Physiology
Ian A. J. Darragh, Sarai Martinez-Pacheco, Lorraine O'Driscoll, Brendan Egan
Summary: This study investigated the effects of plasma from different training groups on BT-549 breast cancer cells. The results showed that supplementing media with plasma from trained athletes did not impact the proliferation, migration, invasion, and anoikis resistance of the cells.
EXPERIMENTAL PHYSIOLOGY
(2023)
Article
Biochemical Research Methods
Anindya Mukhopadhya, Jessie Santoro, Lorraine O'Driscoll
Summary: The translated paragraph discusses the importance of separating highly enriched EV fractions from milk for various studies, highlighting the limitations of current protocols. A new approach combining acid pretreatment and gradient ultracentrifugation is proposed to produce EV-enriched fractions suitable for downstream characterization.
Article
Multidisciplinary Sciences
Sadhbh O'Neill, Richard K. Porter, Niamh McNamee, Vanesa G. Martinez, Lorraine O'Driscoll
SCIENTIFIC REPORTS
(2019)
Article
Oncology
Susan Breslin, Michelle C. Lowry, Lorraine O'Driscoll
BRITISH JOURNAL OF CANCER
(2017)