Review
Cell & Tissue Engineering
Yuling Huang, Xin Li, Lina Yang
Summary: This review summarizes the preclinical studies and clinical trials using MSCs and MSC-sEVs in treating COVID-19, and discusses the molecular mechanism, feasibility, and safety of treating COVID-19 with MSCs and MSC-sEVs.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Cell Biology
Nur Azira Mohd Noor, Asma Abdullah Nurul, Muhammad Rajaei Ahmad Mohd Zain, Wan Khairunnisaa Wan Nor Aduni, Maryam Azlan
Summary: Osteoarthritis is a chronic degenerative disorder of the joint that is becoming more prevalent due to aging populations. Current treatments are limited to painkillers, anti-inflammatory drugs, and surgery for joint replacement. Extracellular vesicles and mesenchymal stem cells show potential for OA treatment, but face challenges in implementation.
Review
Virology
Enrica Serretiello, Andrea Ballini, Annafrancesca Smimmo, Marina Acunzo, Mariarosaria Raimo, Stefania Cantore, Marina Di Domenico
Summary: This article presents the latest evidence on the role of extracellular vesicles (EVs) in viral infections, with a focus on the dual role of exosomes in promoting and inhibiting SARS-CoV-2 infection. The involvement of mesenchymal stromal/stem cells (MSCs) and MSC-derived EVs in COVID-19 treatment is also investigated.
Article
Cell & Tissue Engineering
Ying-Gang Zhu, Meng-meng Shi, Antoine Monsel, Cheng-xiang Dai, Xuan Dong, Hong Shen, Su-ke Li, Jing Chang, Cui-li Xu, Ping Li, Jing Wang, Mei-ping Shen, Cheng-jie Ren, De-chang Chen, Jie-Ming Qu
Summary: This study explores the safety and efficacy of aerosol inhalation of exosomes derived from human adipose-derived MSCs (haMSC-Exos) in COVID-19 patients. The results show that a consecutive 5-day inhalation dose of haMSC-Exos is feasible and well tolerated, with no evidence of adverse events and CT imaging improvement within 7 days.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Immunology
Eliana Lara-Barba, Maria Jesus Araya, Charlotte Nicole Hill, Felipe A. Bustamante-Barrientos, Alexander Ortloff, Cynthia Garcia, Felipe Galvez-Jiron, Carolina Pradenas, Noymar Luque-Campos, Gabriela Maita, Roberto Elizondo-Vega, Farida Djouad, Ana Maria Vega-Letter, Patricia Luz-Crawford
Summary: The study discusses osteoarticular diseases and their treatment options, with a focus on cell therapy using MSCs and acellular therapy based on MSC secreted factors. It highlights the potential therapeutic impact of sEVs derived from MSCs and their miRNA cargo on target cells associated with the pathology of RA and OA.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Bei-Cyuan Guo, Kang-Hsi Wu, Chun-Yu Chen, Wen-Ya Lin, Yu-Jun Chang, Tai-An Lee, Mao-Jen Lin, Han-Ping Wu
Summary: The COVID-19 pandemic has caused significant loss of life due to severe infections. Mesenchymal stem cells (MSCs) have shown potential in mitigating inflammation, modulating immune responses, and promoting tissue regeneration. Accumulating evidence supports the efficacy and safety of MSCs in treating severe COVID-19 and ARDS, but further research is needed to determine optimal administration methods, dosages, treatment intervals, management of extrapulmonary complications, and potential pediatric applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sevindzh Kletukhina, Guzel Mutallapova, Angelina Titova, Marina Gomzikova
Summary: Idiopathic pulmonary fibrosis (IPF) is a progressive disease caused by the aberrant regeneration of pulmonary epithelium. Mesenchymal stem cells (MSCs) and extracellular vesicles (EVs) are promising therapies for pulmonary fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Wenwen Zhang, Tingyu Wang, Yuanye Xue, Bingbing Zhan, Zengjie Lai, Wenjie Huang, Xinsheng Peng, Yanfang Zhou
Summary: There is increasing evidence that mesenchymal stem cell-derived extracellular vesicles and exosomes can effectively improve the treatment of oxidative stress-related diseases. These vesicles and exosomes are rich in bioactive molecules and have various biological regulatory functions. They can reduce oxidative stress and inflammation markers in systemic diseases and treat apoptosis and vascular injury caused by oxidative stress.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dayane Mayumi Miyasaki, Alexandra Cristina Senegaglia, Sergio Adriane Bezerra de Moura, Amanda Leitolis, Luiz Guilherme Achcar Capriglione, Leticia Fracaro, Lidiane Maria Boldrini Leite, Paulo Henrique Utumi, Felipe Yukio Ishikawa Fragoso, Fernando Meyer, Alejandro Correa, Paulo Roberto Slud Brofman
Summary: This study evaluated the effects of extracellular vesicles (EVs) from human umbilical cord tissue and human umbilical cord blood on rats with chronic kidney disease. The results demonstrated that EVs have the potential to improve renal function, suggesting their use as a cell-free treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yan-yan Yan, Wen-min Zhou, Yu-qing Wang, Qiao-ru Guo, Fu-xi Zhao, Zhuang-yan Zhu, Yan-xia Xing, Hai-yan Zhang, Mohamad Aljofan, Alireza Mosavi Jarrahi, Bolat Makabel, Jian-ye Zhang
Summary: SARS-CoV-2 infection has become a global public health emergency, impacting society and the economy. Efforts worldwide are focused on developing vaccines and treatments. Emerging approaches, such as extracellular vesicles (EVs), are being explored to tackle the pandemic, offering potential treatment strategies like MSC-EVs, drug-EVs, vaccine-EVs, platelet-EVs, and others.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Liming Li, Jiafu Mu, Yu Zhang, Chenyang Zhang, Teng Ma, Lu Chen, Tianchen Huang, Jiahe Wu, Jian Cao, Shiqing Feng, Youzhi Cai, Min Han, Jianqing Gao
Summary: This study found that exosomes derived from hypoxic preconditioned human umbilical vein endothelial cells can stimulate angiogenic MSCs, showing great potential in the treatment of traumatic spinal cord injury.
Review
Endocrinology & Metabolism
Soichiro Sonoda, Takayoshi Yamaza
Summary: Systemic transplantation of MSCs is an effective treatment for osteopenia, but the mechanism of action is unclear. Donor MSCs only have a limited presence in the recipient target tissue, suggesting that their direct contribution may not be significant. However, the secretomes and extracellular vesicles (EVs) released from donor MSCs play a key role in disease treatment. This review discusses the contribution of MSC-EVs to bone recovery and introduces a novel mechanism of action of SHED-EVs in osteopenia. It is found that MSC-EVs can rejuvenate the microenvironment and recover bone density by enhancing telomerase activity in recipient BMMSCs. Future challenges in developing reproducible MSC-EV therapy in osteopenia are also addressed.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alireza Yeganeh, Anwar Fathollahi, Seyed Mahmoud Hashemi, Farshid Yeganeh
Summary: The study evaluated the effect of mesenchymal stem cells-derived extracellular vesicles (MSC-EVs) on cytokine production and gene expression related to T helper cell subsets. The treatment with MSC-EV increased the frequency of CD4(+)CD25(+)FOXP3(+) T cells and upregulated Foxp3 mRNA level.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Cell Biology
Yuelong Zhang, Huangming Zhuang, Xunshan Ren, Fuze Jiang, Panghu Zhou
Summary: Osteoarthritis is a common and disabling disease, and surgical treatment is the main approach. Human umbilical cord mesenchymal stem cells (hUC-MSCs) are self-regenerative pluripotent cells that promote cartilage regeneration through the secretion of various trophic factors. The combination of hUC-MSCs with other substances also shows a significant synergistic effect on osteoarthritis treatment. Some scholars have suggested transplanting stem cells into damaged cartilage to slow down the progression of osteoarthritis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Robert Soukup, Iris Gerner, Sinan Gueltekin, Hayeon Baik, Johannes Oesterreicher, Johannes Grillari, Florien Jenner
Summary: Extracellular vesicles (EVs) play an essential role in cellular crosstalk, and equine MSC-derived EVs have comparable therapeutic potential to their donor cells. The International Society for Extracellular Vesicles (ISEV) has proposed standards to define EVs and their functions, but their application in veterinary field is restricted by nonstandardised isolation methods and limited availability of cross-reacting markers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Pharmacology & Pharmacy
Lorraine O'Driscoll, Willem Stoorvogel, Clotilde Thery, Edit Buzas, Irina Nazarenko, Pia Siljander, Maria Yanez-Mo, Stefano Fais, Bernd Giebel, Marjo Yliperttula
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2017)
Article
Biochemical Research Methods
Jan Van Deun, Pieter Mestdagh, Patrizia Agostinis, Ozden Akay, Sushma Anand, Jasper Anckaert, Zoraida Andreu Martinez, Tine Baetens, Els Beghein, Laurence Bertier, Geert Berx, Janneke Boere, Stephanie Boukouris, Michel Bremer, Dominik Buschmann, James B. Byrd, Clara Casert, Lesley Cheng, Anna Cmoch, Delphine Daveloose, Eva De Smedt, Seyma Demirsoy, Victoria Depoorter, Bert Dhondt, Tom A. P. Driedonks, Aleksandra Dudek, Abdou Elsharawy, Ilaria Floris, Andrew D. Foers, Kathrin Gaertner, Abhishek D. Garg, Edward Geeurickx, Jan Gettemans, Farzaneh Ghazavi, Bernd Giebel, Tom Groot Kormelink, Grace Hancock, Hetty Helsmoortel, Andrew F. Hill, Vincent Hyenne, Hina Kalra, David Kim, Joanna Kowal, Sandra Kraemer, Petra Leidinger, Carina Leonelli, Yaxuan Liang, Lien Lippens, Shu Liu, Alessandra Lo Cicero, Shaun Martin, Suresh Mathivanan, Prabhu Mathiyalagan, Tamas Matusek, Gloria Milani, Marta Monguio-Tortajada, Liselot M. Mus, Dillon C. Muth, Andrea Nemeth, Esther N. M. Nolte-'t Hoen, Lorraine O'Driscoll, Roberta Palmulli, Michael W. Pfaffl, Bjarke Primdal-Bengtson, Erminia Romano, Quentin Rousseau, Susmita Sahoo, Natalia Sampaio, Monisha Samuel, Benjamin Scicluna, Bieke Soen, Anneleen Steels, Johannes V. Swinnen, Maarit Takatalo, Safia Thaminy, Clotilde Thery, Joeri Tulkens, Isabel Van Audenhove, Susanne van der Grein, Alan Van Goethem, Martijn J. van Herwijnen, Guillaume Van Niel, Nadine Van Roy, Alexander R. Van Vliet, Niels Vandamme, Suzanne Vanhauwaert, Glenn Vergauwen, Frederik Verweij, Annelynn Wallaert, Marca Wauben, Kenneth W. Witwer, Marijke I. Zonneveld, Olivier De Wever, Jo Vandesompele, An Hendrix
Review
Cell Biology
Mariadelva Catalano, Lorraine O'Driscoll
JOURNAL OF EXTRACELLULAR VESICLES
(2020)
Review
Cell Biology
Dimitrios Tsiapalis, Lorraine O'Driscoll
Review
Pharmacology & Pharmacy
Roisin Daly, Lorraine O'Driscoll
Summary: Extracellular vesicles in the bloodstream could serve as important sources of biomarkers for breast cancer, but the current methods for isolating pure EVs are relatively complex. Research is underway to identify simpler and more selective EV extraction methods, with hope of finding effective biomarkers.
DRUG DISCOVERY TODAY
(2021)
Article
Oncology
Sarai Martinez-Pacheco, Lorraine O'Driscoll
Summary: The characteristics of extracellular vesicles (EVs) can vary depending on the cell line and method of separation used, indicating the importance of considering multiple EV sources when selecting a separation technique for research or clinical purposes.
Article
Oncology
Sarai Martinez-Pacheco, Lorraine O'Driscoll
Summary: The majority of cancer researchers currently rely on two-dimensional (2D) cell culture models due to lack of experience and costs, but they express interest in transitioning to three-dimensional (3D) models, which are considered more clinically relevant. There is an urgent need to standardize reliable, robust, and cost-effective methods for establishing 3D cell culture models in cancer research.
Article
Nutrition & Dietetics
Ian A. J. Darragh, Lorraine O'Driscoll, Brendan Egan
Summary: This study examined within-subject variability in the circulating metabolome under controlled conditions and investigated the association between divergent exercise training backgrounds and alterations in the circulating metabolome in resting samples. The results showed that the reliability of resting plasma metabolite concentrations varied at the level of individual metabolites, and a history of exercise training was associated with alterations in the abundance of certain metabolites.
INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM
(2023)
Article
Pharmacology & Pharmacy
Anindya Mukhopadhya, Dimitrios Tsiapalis, Niamh McNamee, Brian Talbot, Lorraine O'Driscoll
Summary: This study compared the suitability of MSC EVs and milk EVs as drug delivery vehicles. Milk EVs had significantly higher yields compared to MSC EVs. Electroporation was found to be the most effective method for loading the anti-cancer drug Dox into EVs. Additionally, sonication was observed to have detrimental effects on EVs.
Article
Neurosciences
Ian A. J. Darragh, Niamh McNamee, Roisin Daly, Sarai Martinez Pacheco, Lorraine O'Driscoll, Brendan Egan
Summary: This study investigated whether individuals with different exercise training histories had varied abundances of small extracellular vesicles (EVs) in resting samples. The results suggest that the abundance and metabolomic profile of small EVs derived from men with divergent exercise training histories are similar to those in resting blood samples.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Cell & Tissue Engineering
Dimitrios Tsiapalis, Achilleas Floudas, Tobias Tertel, Verena Boerger, Bernd Giebel, Douglas J. Veale, Ursula Fearon, Lorraine O'Driscoll
Summary: This study investigated the effect of human mesenchymal/stromal stem cells and their extracellular vesicles (EVs) on CD4+ T cells from rheumatoid arthritis (RA) patients. The researchers found that EVs from IFN-beta-primed MSCs had a stronger immune-regulatory effect on RA CD4+ T cells compared to MSCs or EVs from naive MSCs. In addition, EVs from IFN-beta-primed MSCs effectively inhibited RA FLS migration and downregulated RA FLS surface markers. These findings suggest that EVs from IFN-beta-primed MSCs could be a potential therapy for RA.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Physiology
Ian A. J. Darragh, Sarai Martinez-Pacheco, Lorraine O'Driscoll, Brendan Egan
Summary: This study investigated the effects of plasma from different training groups on BT-549 breast cancer cells. The results showed that supplementing media with plasma from trained athletes did not impact the proliferation, migration, invasion, and anoikis resistance of the cells.
EXPERIMENTAL PHYSIOLOGY
(2023)
Article
Biochemical Research Methods
Anindya Mukhopadhya, Jessie Santoro, Lorraine O'Driscoll
Summary: The translated paragraph discusses the importance of separating highly enriched EV fractions from milk for various studies, highlighting the limitations of current protocols. A new approach combining acid pretreatment and gradient ultracentrifugation is proposed to produce EV-enriched fractions suitable for downstream characterization.
Article
Multidisciplinary Sciences
Sadhbh O'Neill, Richard K. Porter, Niamh McNamee, Vanesa G. Martinez, Lorraine O'Driscoll
SCIENTIFIC REPORTS
(2019)
Review
Pharmacology & Pharmacy
Karlie R. Sharma, Christine M. Colvis, Griffih P. Rodgers, Douglas M. Sheeley
Summary: There are many genes within the druggable genome that have not been studied, and the US National Institutes of Health's program provides resources to explore these genes, with the potential for rapid impact on human health.
DRUG DISCOVERY TODAY
(2024)
Review
Pharmacology & Pharmacy
Mohammad Sameer Khan, B. H. Jaswanth Gowda, Waleed H. Almalki, Tanuja Singh, Amirhossein Sahebkar, Prashant Kesharwani
Summary: Mitochondria-specific functional liposomes hold great potential for cancer therapy. This review discusses the association between mitochondria and tumor formation, as well as the advantages of liposomes in delivering drugs to mitochondria.
DRUG DISCOVERY TODAY
(2024)
Review
Pharmacology & Pharmacy
Choong Yong Ung, Cristina Correia, Hu Li, Christopher M. Adams, Jennifer J. Westendorf, Shizhen Zhu
Summary: With increasing human life expectancy, the global medical burden of chronic diseases is growing. Chronic diseases often involve malfunctioning of multiple organs, and understanding the interorgan crosstalk is crucial to understanding the etiology of chronic diseases. Researchers have proposed the locked-state model (LoSM) and cutting-edge systems biology and artificial intelligence strategies to decipher chronic multiorgan locked states. The findings have important clinical implications for improving treatments for chronic diseases.
DRUG DISCOVERY TODAY
(2024)
