Recent Advances and Future Perspectives of In Vivo Targeted Delivery of Genome-Editing Reagents to Germ cells, Embryos, and Fetuses in Mice

Transplacental Delivery of Genome Editing Components Causes Mutations in Embryonic Cardiomyocytes of Mid-Gestational Murine Fetuses

(2019) Shingo Nakamura et al.   IUBMB LIFE

In utero gene editing for monogenic lung disease

(2019) Deepthi Alapati et al.   Science Translational Medicine

i ‐ GONAD : A method for generating genome‐edited animals without ex vivo handling of embryos

(2019) Masato Ohtsuka et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD

(2019) Channabasavaiah B. Gurumurthy et al.   Nature Protocols

CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection

(2019) Sean Chen et al.   Cell Reports

Genome-Editing Technologies: Concept, Pros, and Cons of Various Genome-Editing Techniques and Bioethical Concerns for Clinical Application

(2019) Sikandar Hayat Khan   Molecular Therapy-Nucleic Acids

Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity

(2019) Yuanyan Tan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Restore natural fertility of Kitw/Kitwv mouse with nonobstructive azoospermia through gene editing on SSCs mediated by CRISPR-Cas9

(2019) Xiaoyu Li et al.   Stem Cell Research & Therapy

Transplacental Gene Delivery (TPGD) as a Noninvasive Tool for Fetal Gene Manipulation in Mice

(2019) Nakamura et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Successful production of genome-edited rats by the rGONAD method

(2018) Tomoe Kobayashi et al.   BMC BIOTECHNOLOGY

Delivering CRISPR: a review of the challenges and approaches

(2018) Christopher A. Lino et al.   DRUG DELIVERY

Reproductive technologies for the generation and maintenance of valuable animal strains

(2018) Takehito KANEKO   JOURNAL OF REPRODUCTION AND DEVELOPMENT

Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos

(2018) Bin Gu et al.   NATURE BIOTECHNOLOGY

Efficient mouse genome engineering by CRISPR-EZ technology

(2018) Andrew J Modzelewski et al.   Nature Protocols

An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

(2018) Simon E. Tröder et al.   PLoS One

Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses

(2018) Yeonsoo Yoon et al.   Nature Communications

In utero nanoparticle delivery for site-specific genome editing

(2018) Adele S. Ricciardi et al.   Nature Communications

Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing

(2018) Marie Teixeira et al.   Scientific Reports

i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats

(2018) Shuji Takabayashi et al.   Scientific Reports

In utero CRISPR-mediated therapeutic editing of metabolic genes

(2018) Avery C. Rossidis et al.   NATURE MEDICINE

Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes

(2017) Ashley M. Jacobi et al.   METHODS

Development of fertile mouse oocytes from mitotic germ cells in vitro

(2017) Kanako Morohaku et al.   Nature Protocols

Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote

(2017) Aurélien Raveux et al.   Scientific Reports

Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes

(2016) Van Trung Chu et al.   BMC BIOTECHNOLOGY

Developing ade novotargeted knock-in method based onin uteroelectroporation into the mammalian brain

(2016) Yuji Tsunekawa et al.   DEVELOPMENT

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Complete in vitro generation of fertile oocytes from mouse primordial germ cells

(2016) Kanako Morohaku et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques

(2016) Takeshi Uemura et al.   Scientific Reports

Nonsense-Mediated mRNA Decay: Degradation of Defective Transcripts Is Only Part of the Story

(2015) Feng He et al.   Annual Review of Genetics

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice

(2015) Gou Takahashi et al.   Scientific Reports

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9

(2015) Takuya Sato et al.   Stem Cell Reports

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

(2015) Masakazu Hashimoto et al.   Scientific Reports

Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells

(2014) Yuxuan Wu et al.   CELL RESEARCH

A microdevice to locally electroporate embryos with high efficiency and reduced cell damage

(2014) E. Mazari et al.   DEVELOPMENT

Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes

(2014) Shuo-Ting Yen et al.   DEVELOPMENTAL BIOLOGY

Dual sgRNAs facilitate CRISPR/Cas9-mediated mouse genome targeting

(2014) Jiankui Zhou et al.   FEBS Journal

Genome Editing in Mouse Spermatogonial Stem/Progenitor Cells Using Engineered Nucleases

(2014) Danielle A. Fanslow et al.   PLoS One

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering

(2014) Takuro Horii et al.   Scientific Reports

Simple knockout by electroporation of engineered endonucleases into intact rat embryos

(2014) Takehito Kaneko et al.   Scientific Reports

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Generation of gene-modified mice via Cas9/RNA-mediated gene targeting

(2013) Bin Shen et al.   CELL RESEARCH

Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9

(2013) Yuxuan Wu et al.   Cell Stem Cell

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

(2013) Y. H. Sung et al.   GENOME RESEARCH

Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

(2013) Dali Li et al.   NATURE BIOTECHNOLOGY

Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease

(2013) Wataru Fujii et al.   NUCLEIC ACIDS RESEARCH

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

(2013) Daisuke Mashiko et al.   Scientific Reports

Characterization of Spermatogonial Stem Cells Lacking Intercellular Bridges and Genetic Replacement of a Mutation in Spermatogonial Stem Cells

(2012) Naoki Iwamori et al.   PLoS One

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

The neural crest-enriched microRNA miR-452 regulates epithelial-mesenchymal signaling in the first pharyngeal arch

(2010) N. T. Sheehy et al.   DEVELOPMENT

Ex vivo magnetofection: A novel strategy for the study of gene function in mouse organogenesis

(2009) Terje Svingen et al.   DEVELOPMENTAL DYNAMICS

Pluripotency of a Single Spermatogonial Stem Cell in Mice1

(2008) Mito Kanatsu-Shinohara et al.   BIOLOGY OF REPRODUCTION