Creation of CRISPR-based germline-genome-engineered mice without ex vivo handling of zygotes by i-GONAD

Successful production of genome-edited rats by the rGONAD method

(2018) Tomoe Kobayashi et al.   BMC BIOTECHNOLOGY

An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes

(2018) Simon E. Tröder et al.   PLoS One

Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing

(2018) Marie Teixeira et al.   Scientific Reports

i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats

(2018) Shuji Takabayashi et al.   Scientific Reports

Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors

(2017) Hiromi Miura et al.   Nature Protocols

A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular ResearchHighlights

(2016) Joseph M. Miano et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse

(2016) Masakazu Hashimoto et al.   DEVELOPMENTAL BIOLOGY

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Breaking-Cas—interactive design of guide RNAs for CRISPR-Cas experiments for ENSEMBL genomes

(2016) Juan C. Oliveros et al.   NUCLEIC ACIDS RESEARCH

CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering

(2016) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA

(2015) Hiromi Miura et al.   Scientific Reports

GONAD: Genome-editing via Oviductal Nucleic Acids Delivery system: a novel microinjection independent genome engineering method in mice

(2015) Gou Takahashi et al.   Scientific Reports

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice

(2015) Tomomi Aida et al.   GENOME BIOLOGY

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

A conditional knockout resource for the genome-wide study of mouse gene function

(2011) William C. Skarnes et al.   NATURE

TAL Effectors: Customizable Proteins for DNA Targeting

(2011) A. J. Bogdanove et al.   SCIENCE

The construction of transgenic and gene knockout/knockin mouse models of human disease

(2011) Alfred Doyle et al.   TRANSGENIC RESEARCH

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression

(2010) Masato Ohtsuka et al.   NUCLEIC ACIDS RESEARCH

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

(2009) Aron M. Geurts et al.   SCIENCE

An efficient method for purification of PCR products for sequencing

(2008) Hao Ma et al.   BIOTECHNIQUES