Article
Zoology
Cong Li, Rui Bi, Lin Wang, Yu-Hua Ma, Yong-Gang Yao, Ping Zheng
Summary: In this study, the in vivo spermatogenesis potential of tree shrew SSCs cultured across different passages was examined. It was found that long-term expansion (>50 passages) led to the loss of spermatogenesis ability in SSCs. RNA sequencing analysis revealed significant gene expression changes after sub-culturing primary SPG lines for more than 40 passages, and experimental validation confirmed DNA damage accumulation and mitochondrial dysfunction in high-passage cells. Supplementation with NAD(+) precursor NR showed beneficial effects in reducing DNA damage accumulation and mitochondrial dysfunction. This research provides a comprehensive analysis of the genetic and physiological attributes critical for the sustained expansion of undifferentiated SSCs in tree shrews and proposes an effective strategy for extended in vitro maintenance.
ZOOLOGICAL RESEARCH
(2023)
Article
Cell Biology
Xiaomin Du, Siyu Wu, Yudong Wei, Xiuwei Yu, Fanglin Ma, Yuanxin Zhai, Donghui Yang, Mengfei Zhang, Wenqing Liu, Haijing Zhu, Jiang Wu, Mingzhi Liao, Na Li, Chunling Bai, Guangpeng Li, Jinlian Hua
Summary: The study identified a subset of spermatogonial stem cells in dairy goats with high PAX7 expression, indicating that PAX7 can maintain the self-renewal of these cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Reproductive Biology
Sang-Eun Jung, Ju-Hee Jin, Jin Seop Ahn, Yong-Hee Kim, Min-Hyung Yun, Seo-Hee Kim, Bang-Jin Kim, Buom-Yong Ryu
Summary: The study demonstrated that cryopreservation of SSCs with 5% rHSA showed higher relative proliferation potential and significantly reduced early apoptosis compared to DMSO control. Additionally, SSCs cryopreserved with 5% rHSA exhibited normal self-renewal and differentiation abilities.
Review
Endocrinology & Metabolism
Lei Diao, Paul J. Turek, Constance M. John, Fang Fang, Renee A. Reijo Pera
Summary: Spermatogonial stem cells (SSCs) are essential adult stem cells involved in spermatogenesis and male fertility. Understanding the regulation of SSCs is crucial for studying their function and the causes of male infertility, as well as developing new treatments through in vitro culture and differentiation of SSCs. This review highlights the recent research progress on SSC regulation and discusses the potential application of SSCs in fertility restoration.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Medicine, Research & Experimental
Fatemeh Sokouti Nasimi, Saber Zahri, Shahin Ahmadian, Afsaneh Bagherzadeh, Nahideh Nazdikbin Yamchi, Leila Haghighi, Alberto Miranda Bedate, Balal Khalilzadeh, Reza Rahbarghazi, Mahdi Mahdipour
Summary: The study showed that estradiol can increase the viability of mouse SSCs in a dose-dependent manner, leading to changes in cell morphology and gene expression, as well as reduced sperm parameters and testicular progenitor cells. This suggests that estradiol may induce Sertoli cell differentiation of SSCs while long-term exogenous administration can lead to infertility.
Article
Cell & Tissue Engineering
Xiaotong Wang, Mengyuan Qu, Zili Li, Yuting Long, Kai Hong, Honggang Li
Summary: The study developed a protocol to induce spermatogonial stem cell-like cells (SSCLCs) from human pluripotent stem cells, revealing that a combination of low concentrations of VPA and VC was most effective in inducing SSCLCs expressing spermatogonial genes. RNA-seq analysis showed upregulated genes in the Wnt signaling pathway during SSCLC induction. Additionally, hiPSC lines from non-obstructive azoospermia (NOA) patients exhibited decreased SSCLC induction efficiency and reduced expression of genes in the Wnt signaling pathway. The study suggests that the inactivation of the Wnt signaling pathway may be associated with SSC depletion in azoospermia testes.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Dai Zhou, Jingyu Fan, Zhizhong Liu, Ruiling Tang, Xingming Wang, Hao Bo, Fang Zhu, Xueheng Zhao, Zenghui Huang, Liu Xing, Ke Tao, Han Zhang, Hongchuan Nie, Huan Zhang, Wenbing Zhu, Zuping He, Liqing Fan
Summary: This study reveals that TCF3 regulates the proliferation and apoptosis of human SSCs via targeting PODXL, providing a novel molecular mechanism for understanding the fate determinations of human SSCs and potential new targets for gene therapy of male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Andrology
Shu-Wei Luo, Le Tang, Dai Zhou, Hao Bo, Li-Qing Fan
Summary: FOXP4 expression gradually increased during the development of human SSCs. It specifically marks a subset of spermatogonia with stem cell potential. Inactivation of FOXP4 suppressed SSC proliferation and activated apoptosis. These findings suggest the involvement of FOXP4 in human SSC proliferation and provide insights into the mechanisms controlling fate decisions in human SSCs.
ASIAN JOURNAL OF ANDROLOGY
(2023)
Review
Cell Biology
Nesma E. Abdelaal, Bereket Molla Tanga, Mai Abdelgawad, Sahar Allam, Mostafa Fathi, Islam M. Saadeldin, Seonggyu Bang, Jongki Cho
Summary: Male infertility is a major health issue affecting a significant percentage of couples worldwide. The basis of spermatogenesis lies in spermatogonial stem cells, which hold promise for the regeneration of impaired spermatogenesis. However, challenges such as cryopreservation efficiency, malignant cell contamination, and socio-cultural attitudes hinder the wider application of SSCs as alternatives for the treatment of male infertility.
Article
Cell Biology
Dai Zhou, Jingyu Fan, Zhizhong Liu, Ruiling Tang, Xingming Wang, Hao Bo, Fang Zhu, Xueheng Zhao, Zenghui Huang, Liu Xing, Ke Tao, Han Zhang, Hongchuan Nie, Huan Zhang, Wenbing Zhu, Zuping He, Liqing Fan
Summary: The gene TCF3 was found to promote proliferation and inhibit apoptosis of human SSCs by targeting PODXL, offering new targets for gene therapy of male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Maryam Khanehzad, Seyed Mehdi Nourashrafeddin, Farid Abolhassani, Shokoofeh Kazemzadeh, Soheila Madadi, Elham Shiri, Parastoo Khanlari, Zahra Khosravizadeh, Azim Hedayatpour
Summary: The study found that inhibition of miRNA-30a-5p promotes differentiation and reduces proliferation markers expression in SSCs. This provides new clues for understanding the role of miRNAs in fate determinations of SSCs.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Amirreza Niazi Tabar, Hossein Azizi, Danial Hashemi Karoii, Thomas Skutella
Summary: This study examined the expression of vimentin in vivo and in vitro and found that vimentin plays important roles in the differentiation of testicular germ cells. The results suggest that vimentin is an intermediate filament with crucial roles in the differentiation stages of spermatogenesis.
Article
Engineering, Biomedical
Fatemeh Asgari, Hamid Reza Asgari, Mohammad Najafi, Behnaz Sadat Eftekhari, Mina Vardiani, Mazaher Gholipourmalekabadi, Morteza Koruji
Summary: The decellularized scaffold with 0.5%/30 SDS/Triton was found to have minimal negative effects on ECM, making it an excellent platform for tissue engineering applications. Different concentrations and incubation times had varying effects on swelling ratios and cell viability. The colony formation of spermatogonial stem cells was clearly observed within the scaffold.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2021)
Article
Agriculture, Dairy & Animal Science
Leilei Tan, Qian Liu, Yangbin He, Jingjing Zhang, Jilun Hou, Yuqin Ren, Wenxiu Ma, Qian Wang, Changwei Shao
Summary: In this study, a spermatogenic stem cell line was established from tiger puffer fish, which showed stable growth and the ability to differentiate into other types of cells. This research provides an important research model and foundation for sex determination and breeding control in fish.
Article
Agriculture, Dairy & Animal Science
Xin Zhao, Weican Wan, Bin Li, Xianyu Zhang, Mao Zhang, Zhenfang Wu, Huaqiang Yang
Summary: Spermatogonial stem cells (SSCs) are adult stem cells capable of passing genetic information to offspring through self-renewal and differentiation into mature spermatozoa. Optimal in vitro culture of SSCs can benefit various studies and applications related to SSC biology.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)