Article
Gastroenterology & Hepatology
Xu Zhilu, Dong Xiangqian, Yang Keli, Chevarin Caroline, Zhang Jingwan, Lin Yu, Zuo Tao, Chu Lok Cheung, Sun Yang, Zhang Fengrui, Chan Francis Kl, Sung Joseph Jy, Yu Jun, Buisson Anthony, Barnich Nicolas, Colombel Jean-Frederic, Wong Sunny Hei, Miao Yinglei, Ng C. Siew
Summary: The study found a high prevalence of AIEC in CD patients in Hong Kong, which was associated with severe microbiota dysbiosis and impeded restoration of normal gut microbiota. AIEC may serve as a keystone bacterium in CD and impact the efficacy of FMT.
Review
Medicine, General & Internal
Daisuke Watanabe, Nobuhiko Kamada
Summary: This review summarizes the current understanding of the link between intestinal fibrosis in CD and the gut microbiota. It highlights the role of gut microbiota in the development of intestinal fibrosis in CD and provides valuable information about animal models used in investigating this condition.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Guillaume Dalmasso, Hang Thi Thu Nguyen, Tiphanie Fais, Sebastien Massier, Caroline Chevarin, Emilie Vazeille, Nicolas Barnich, Julien Delmas, Richard Bonnet
Summary: The study revealed that the yersiniabactin siderophore of AIEC provides an advantage for growth in competitive environments, but it also activates autophagy leading to bacterial clearance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Waleska Saitz, David A. Montero, Mirka Pardo, Daniela Araya, Marjorie De la Fuente, Marcela A. Hermoso, Mauricio J. Farfan, Daniel Ginard, Ramon Rossello-Mora, Dave A. Rasko, Felipe Del Canto, Roberto M. Vidal
Summary: The study aimed to identify molecular markers for screening AIEC strains. By analyzing outer membrane proteins and genes in AIEC strains, the study found that chuA, eefC, and fitA genes are significantly correlated with AIEC strains and can serve as markers for AIEC strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Imteyaz Ahmad Khan, Baibaswata Nayak, Manasvini Markandey, Aditya Bajaj, Mahak Verma, Sambudhha Kumar, Mukesh Kumar Singh, Saurabh Kedia, Vineet Ahuja
Summary: Crohn's disease and Intestinal tuberculosis are chronic intestinal diseases with similar phenotype but diverse etiologies. This study found a higher prevalence of host gene polymorphisms and pathobionts in colonic mucosa of patients with CD compared to ITB.
Article
Cell Biology
Adeline Sivignon, Melissa Chervy, Caroline Chevarin, Elia Ragot, Elisabeth Billard, Jeremy Denizot, Nicolas Barnich
Summary: The overexpression of glycoprotein CEACAM6 is associated with the gut establishment of adherent-invasive Escherichia coli (AIEC) in the ileal epithelium. By generating a transgenic mouse model, researchers found that CEACAM6 is strongly expressed in the small intestine mucosa and is correlated with glycosylations of enterocytes. The study also showed that CEACAM6 expression enhances the interaction between AIEC and enterocytes and requires the presence of the bacterial adhesive factor FimH. This preclinical model provides new insights into the mechanistic study of AIEC pathobiont and offers a valuable tool for evaluating strategies to eliminate AIEC in the ileal mucosa.
DISEASE MODELS & MECHANISMS
(2022)
Article
Microbiology
Elio Rossi, Gabriella Leccese, Valerio Baldelli, Alessia Bibi, Emanuele Scalone, Carlo Camilloni, Moira Paroni, Paolo Landini
Summary: The pyrD gene plays a crucial role in the virulence of adherent-invasive Escherichia coli (AIEC), and there are structural differences between E. coli and human DHOD, making it a promising target for therapeutic strategies against chronic inflammation in Crohn's disease (CD).
Article
Microbiology
Monica Viladomiu, Maeva L. Metz, Svetlana F. Lima, Wen-Bing Jin, Lance Chou, Jri Live Cell Bank, Chun-Jun Guo, Gretchen E. Diehl, Kenneth W. Simpson, Ellen J. Scherl, Randy S. Longman
Summary: This study revealed the association of AIEC encoding PduC with the microbiome of CD patients, driving AIEC-induced intestinal T cell inflammation. The inflammatory cascade requires the catalytic activity of PduC to generate propionate, synergizing with lipopolysaccharide to induce IL-1 beta by MNPs. Disrupting fucose availability can limit AIEC-induced propionate production and intestinal inflammation.
CELL HOST & MICROBE
(2021)
Article
Microbiology
Valentina Lucchini, Adeline Sivignon, Michel Pieren, Marc Gitzinger, Sergio Lociuro, Nicolas Barnich, Christian Kemmer, Vincent Trebosc
Summary: The OmpR transcriptional regulator, while not conservatively involved in adhesion in vitro, was found to be necessary for robust gut colonization in AIEC strains and may be involved in alternative virulence mechanisms. Additionally, OmpR was shown to play a crucial role in AIEC strains' ability to cope with stress induced by bile salts, specifically in tolerating sodium deoxycholate.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Pathology
Laura Tuomisto, Ravi Kant, Anna-Mariam Kiviranta, Kukka-Maaria Helkioe, Tarja Sironen, Antti Sukura, Rebecca P. Wilkes, Kristel Kegler
Summary: This case report describes a case of granulomatous colitis (GC) associated with adherent-invasive Escherichia coli (AIEC) that spread to various organs in a 10-year-old, male Sphynx cat. The cat initially experienced diarrhea and sudden blindness before rapidly progressing to ataxia, seizures, and death. Inflammation consistent with granulomatous inflammation was found in all affected organs, and the presence of AIEC within enterocytes and infiltrating macrophages was confirmed.
VETERINARY PATHOLOGY
(2023)
Article
Microbiology
Dalmasso Guillaume, Beyrouthy Racha, Brugiroux Sandrine, Ruppe Etienne, Guillouard Laurent, Bonnin Virginie, Saint-Sardos Pierre, Ghozlane Amine, Gaumet Vincent, Barnich Nicolas, Delmas Julien, Bonnet Richard
Summary: Without the use of antibiotics, the plasmid-mediated resistance gene mcr-1 enhances the colonization ability of Escherichia coli in the gut, but impairs its lethal effect. This improvement in gut fitness is associated with a downregulation of intestinal inflammatory markers and the preservation of intestinal microbiota composition.
Article
Microbiology
Qingyuan Yi, Jiaxin Liu, Yufeng Zhang, Hanzhen Qiao, Fang Chen, Shihai Zhang, Wutai Guan
Summary: This study demonstrated that dietary supplementation of anethole can improve the growth performance of weaned piglets challenged with enterotoxigenic Escherichia coli K88 by attenuating intestinal barrier disruption and inflammation.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Bin Liu, Lingyan Jiang, Yutao Liu, Hongmin Sun, Jun Yan, Chenbo Kang, Bin Yang
Summary: The mammalian gastrointestinal tract is a complex environment that hosts a diverse microbial community. This study discovered that enterohemorrhagic Escherichia coli (EHEC) O157:H7 imports L-malate from the host and microbiota using the DcuABC transporters, converting it into fumarate to fuel anaerobic respiration and promote colonization. L-malate also acts as a signaling molecule to activate virulence gene expression in EHEC O157:H7.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Yi Yang, Mytien Nguyen, Varnica Khetrapal, Nicole D. Sonnert, Anjelica L. Martin, Haiwei Chen, Martin A. Kriegel, Noah W. Palm
Summary: This study reveals that within-host evolution of gut commensal bacteria plays a critical role in bacterial translocation and initiation of inflammation. Gut microbiota strains can adapt and evolve, affecting their propensity to elicit inflammatory diseases. The changes in bacterial behavior are associated with genetic mutations, altered microbial gene expression programs, and remodelled cell wall structures.
Article
Microbiology
Hatem Kittana, Joao C. Gomes-Neto, Kari Heck, Anthony F. Juritsch, Jason Sughroue, Yibo Xian, Sara Mantz, Rafael Segura R. Munoz, Liz A. Cody, Robert J. Schmaltz, Christopher L. Anderson, Rodney A. Moxley, Jesse M. Hostetter, Samodha C. Fernando, Jennifer Clarke, Stephen D. Kachman, Clayton E. Cressler, Andrew K. Benson, Jens Walter, Amanda E. Ramer-Tait
Summary: Inflammatory bowel disease (IBD) is associated with an altered gut microbiota composition, including expansion of Proteobacteria. Adherent-invasive Escherichia coli (AIEC) strains are enriched in some IBD patients, and AIEC strains show enhanced pathogenicity compared to non-AIEC strains. AIEC strains cause more severe intestinal inflammation and intracellular survival/replication phenotypes positively correlate with disease. This knowledge can be therapeutically exploited to alleviate intestinal inflammation.
Article
Gastroenterology & Hepatology
Adeline Sivignon, Amelie de Vallee, Nicolas Barnich, Jeremy Denizot, Claude Darcha, Georges Pignede, Pascal Vandekerckove, Arlette Darfeuille-Michaud
INFLAMMATORY BOWEL DISEASES
(2015)
Article
Multidisciplinary Sciences
Rachel Fellows, Jeremy Denizot, Claudia Stellato, Alessandro Cuomo, Payal Jain, Elena Stoyanova, Szabina Balazsi, Zoltan Hajnady, Anke Liebert, Juri Kazakevych, Hector Blackburn, Renan Oliveira Correa, Jose Luis Fachi, Fabio Takeo Sato, Willian R. Ribeiro, Caroline Marcantonio Ferreira, Helene Peree, Mariangela Spagnuolo, Raphael Mattiuz, Csaba Matolcsi, Joana Guedes, Jonathan Clark, Marc Veldhoen, Tiziana Bonaldi, Marco Aurelio Ramirez Vinolo, Patrick Varga-Weisz
NATURE COMMUNICATIONS
(2018)
Article
Gastroenterology & Hepatology
Margarita Martinez-Medina, Jeremy Denizot, Nicolas Dreux, Frederic Robin, Elisabeth Billard, Richard Bonnet, Arlette Darfeuille-Michaud, Nicolas Barnich
Article
Gastroenterology & Hepatology
Jeremy Denizot, Alexis Desrichard, Allison Agus, Nancy Uhrhammer, Nicolas Dreux, Valerie Vouret-Craviari, Paul Hofman, Arlette Darfeuille-Michaud, Nicolas Barnich
Article
Gastroenterology & Hepatology
Jeremy Denizot, Adeline Sivignon, Frederick Barreau, Claude Darcha, H. F. Carlos Chan, Clifford P. Stanners, Paul Hofman, Arlette Darfeuille-Michaud, Nicolas Barnich
INFLAMMATORY BOWEL DISEASES
(2012)
Article
Multidisciplinary Sciences
Allison Agus, Jeremy Denizot, Jonathan Thevenot, Margarita Martinez-Medina, Sebastien Massier, Pierre Sauvanet, Annick Bernalier-Donadille, Sylvain Denis, Paul Hofman, Richard Bonnet, Elisabeth Billard, Nicolas Barnich
SCIENTIFIC REPORTS
(2016)
Article
Microbiology
Nicolas Dreux, Jeremy Denizot, Margarita Martinez-Medina, Alexander Mellmann, Maria Billig, Dagmara Kisiela, Sujay Chattopadhyay, Evgeni Sokurenko, Christel Neut, Corinne Gower-Rousseau, Jean-Frederic Colombel, Richard Bonnet, Arlette Darfeuille-Michaud, Nicolas Barnich
Article
Medicine, General & Internal
Jeremy Denizot, Nicolas Barnich
Article
Multidisciplinary Sciences
Elodie Gimier, Melissa Chervy, Allison Agus, Adeline Sivignon, Elisabeth Billard, Maud Privat, Sandrine Viala, Regine Minet-Quinard, Anthony Buisson, Emilie Vazeille, Nicolas Barnich, Jeremy Denizot
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Adeline Sivignon, Melissa Chervy, Caroline Chevarin, Elia Ragot, Elisabeth Billard, Jeremy Denizot, Nicolas Barnich
Summary: The overexpression of glycoprotein CEACAM6 is associated with the gut establishment of adherent-invasive Escherichia coli (AIEC) in the ileal epithelium. By generating a transgenic mouse model, researchers found that CEACAM6 is strongly expressed in the small intestine mucosa and is correlated with glycosylations of enterocytes. The study also showed that CEACAM6 expression enhances the interaction between AIEC and enterocytes and requires the presence of the bacterial adhesive factor FimH. This preclinical model provides new insights into the mechanistic study of AIEC pathobiont and offers a valuable tool for evaluating strategies to eliminate AIEC in the ileal mucosa.
DISEASE MODELS & MECHANISMS
(2022)
Article
Gastroenterology & Hepatology
Melissa Chervy, Adeline Sivignon, Flavie Dambrine, Anthony Buisson, Pierre Sauvanet, Catherine Godfraind, Matthieu Allez, Lionel Le Bourhis, Nicolas Barnich, Jeremy Denizot
Summary: Histone deacetylases (HDAC) expression in intestinal epithelial cells of Crohn's disease (CD) patients regulates the entry of adherent-invasive Escherichia coli (AIEC) bacteria to the intestinal mucosa. A western diet affects HDAC expression, enabling AIEC colonization and manipulation of the host epigenome.
Article
Biotechnology & Applied Microbiology
Juri Kazakevych, Jeremy Denizot, Anke Liebert, Mariana Portovedo, Mia Mosavie, Payal Jain, Claudia Stellato, Claire Fraser, Renan Oliveira Correa, Marina Celestine, Raphael Mattiuz, Hanneke Okkenhaug, J. Ross Miller, Marco Aurelio Ramirez Vinolo, Marc Veldhoen, Patrick Varga-Weisz
Article
Gastroenterology & Hepatology
Gwladys Sevrin, Sebastien Massier, Benoit Chassaing, Allison Agus, Julien Delmas, Jeremy Denizot, Elisabeth Billard, Nicolas Barnich
Article
Biotechnology & Applied Microbiology
Hashem Koohy, Daniel J. Bolland, Louise S. Matheson, Stefan Schoenfelder, Claudia Stellato, Andrew Dimond, Csilla Varnai, Peter Chovanec, Tamara Chessa, Jeremy Denizot, Raquel Manzano Garcia, Steven W. Wingett, Paula Freire-Pritchett, Takashi Nagano, Phillip Hawkins, Len Stephens, Sarah Elderkin, Mikhail Spivakov, Peter Fraser, Anne E. Corcoran, Patrick D. Varga-Weisz