Methods for protein delivery into cells: from current approaches to future perspectives

Internalization mechanisms of cell-penetrating peptides

(2020) Ivana Ruseska et al.   Beilstein Journal of Nanotechnology

Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules

(2019) Yuhong Cao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

On-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes

(2019) Jian-An Huang et al.   NANO LETTERS

Membrane Electroporation and Electropermeabilization: Mechanisms and Models

(2019) Tadej Kotnik et al.   Annual Review of Biophysics

MoNa – A Cost-Efficient, Portable System for the Nanoinjection of Living Cells

(2019) Matthias Simonis et al.   Scientific Reports

Multifunctional Branched Nanostraw-Electroporation Platform for Intracellular Regulation and Monitoring of Circulating Tumor Cells

(2019) Gen He et al.   NANO LETTERS

Nanoscale Imaging of Primary Cilia with Scanning Ion Conductance Microscopy

(2018) Yuanshu Zhou et al.   ANALYTICAL CHEMISTRY

Imaging of native transcription factors and histone phosphorylation at high resolution in live cells

(2018) Sascha Conic et al.   JOURNAL OF CELL BIOLOGY

Monoclonal Cell Line Generation and CRISPR/Cas9 Manipulation via Single-Cell Electroporation

(2018) Ruiguo Yang et al.   Small

Saponins as Natural Adjuvant for Antisense Morpholino Oligonucleotides Delivery In Vitro and in mdx Mice

(2018) Mingxing Wang et al.   Molecular Therapy-Nucleic Acids

Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts

(2018) Martin P. Stewart et al.   CHEMICAL REVIEWS

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

(2018) Duverney Chaverra-Rodriguez et al.   Nature Communications

Combined Numerical and Experimental Investigation of Localized Electroporation-Based Cell Transfection and Sampling

(2018) Prithvijit Mukherjee et al.   ACS Nano

The Endosomal Escape of Nanoparticles: Toward More Efficient Cellular Delivery

(2018) Samuel A. Smith et al.   BIOCONJUGATE CHEMISTRY

Surface Fluctuations and Coalescence of Nucleolar Droplets in the Human Cell Nucleus

(2018) Christina M. Caragine et al.   PHYSICAL REVIEW LETTERS

Universal intracellular biomolecule delivery with precise dosage control

(2018) Y. Cao et al.   Science Advances

Vector-free intracellular delivery by reversible permeabilization

(2017) Shirley O’Dea et al.   PLoS One

Microinjection of Antibodies Targeting the Lamin A/C Histone-Binding Site Blocks Mitotic Entry and Reveals Separate Chromatin Interactions with HP1, CenpB and PML

(2017) Charles Dixon et al.   Cells

A novel rapid and reproducible flow cytometric method for optimization of transfection efficiency in cells

(2017) Stefanie Homann et al.   PLoS One

Survival rate of eukaryotic cells following electrophoretic nanoinjection

(2017) Matthias Simonis et al.   Scientific Reports

Angular Approach Scanning Ion Conductance Microscopy

(2016) Andrew Shevchuk et al.   BIOPHYSICAL JOURNAL

A precise pointing nanopipette for single-cell imaging via electroosmotic injection

(2016) Jian Lv et al.   CHEMICAL COMMUNICATIONS

A High-Throughput Automated Microinjection System for Human Cells With Small Size

(2016) Yu Ting Chow et al.   IEEE-ASME TRANSACTIONS ON MECHATRONICS

Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation

(2016) Edward H. Hinchcliffe et al.   NATURE CELL BIOLOGY

High-Efficiency Gene Transfection of Cells through Carbon Nanotube Arrays

(2016) Masoud Golshadi et al.   Small

Macromolecular Crowding In Vitro , In Vivo , and In Between

(2016) Germán Rivas et al.   TRENDS IN BIOCHEMICAL SCIENCES

Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

(2016) C. D. Richardson et al.   Nature Communications

A Flow-Through Cell Electroporation Device for Rapidly and Efficiently Transfecting Massive Amounts of Cells in vitro and ex vivo

(2016) Deyao Zhao et al.   Scientific Reports

Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation

(2015) Mindaugas S. Venslauskas et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Comparison of Atomic Force Microscopy and Scanning Ion Conductance Microscopy for Live Cell Imaging

(2015) Jan Seifert et al.   LANGMUIR

Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes

(2015) Simon Hennig et al.   NANO LETTERS

On-chip electroporation and impedance spectroscopy of single-cells

(2015) Sebastian C. Bürgel et al.   SENSORS AND ACTUATORS B-CHEMICAL

Challenges in carrier-mediated intracellular delivery: moving beyond endosomal barriers

(2015) Martin P. Stewart et al.   Wiley Interdisciplinary Reviews-Nanomedicine and Nanobiotechnology

Electroporation on microchips: the harmful effects of pH changes and scaling down

(2015) Yang Li et al.   Scientific Reports

Microinjection of specific anti-IMPDH2 antibodies induces disassembly of cytoplasmic rods/rings that are primarily stationary and stable structures

(2015) Gerson Keppeke et al.   Cell and Bioscience

Promises and Pitfalls of Intracellular Delivery of Proteins

(2014) Ailing Fu et al.   BIOCONJUGATE CHEMISTRY

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Imaging Single Nanoparticle Interactions with Human Lung Cells Using Fast Ion Conductance Microscopy

(2014) Pavel Novak et al.   NANO LETTERS

Single Cell Transfection with Single Molecule Resolution Using a Synthetic Nanopore

(2014) Volker Kurz et al.   NANO LETTERS

When less becomes more: Optimization of protein expression in HEK293–EBNA1 cells using plasmid titration – A case study for NLRs

(2014) Els F. Halff et al.   PROTEIN EXPRESSION AND PURIFICATION

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

Nanostraw–Electroporation System for Highly Efficient Intracellular Delivery and Transfection

(2013) Xi Xie et al.   ACS Nano

Direct Delivery of Functional Proteins and Enzymes to the Cytosol Using Nanoparticle-Stabilized Nanocapsules

(2013) Rui Tang et al.   ACS Nano

Emergence of a large pore subpopulation during electroporating pulses

(2013) Kyle C. Smith et al.   BIOELECTROCHEMISTRY

Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins

(2013) Seung Woo Cho et al.   GENETICS

Polyprodrug Amphiphiles: Hierarchical Assemblies for Shape-Regulated Cellular Internalization, Trafficking, and Drug Delivery

(2013) Xianglong Hu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Nanofountain Probe Electroporation (NFP-E) of Single Cells

(2013) Wonmo Kang et al.   NANO LETTERS

Fast Translocation of Proteins through Solid State Nanopores

(2013) Calin Plesa et al.   NANO LETTERS

Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels

(2013) Pavel Novak et al.   NEURON

Flow-Through Comb Electroporation Device for Delivery of Macromolecules

(2012) Andrea Adamo et al.   ANALYTICAL CHEMISTRY

Visualization of electroporation-induced temperature rise using temperature-sensitive ink

(2012) Kosaku Kurata et al.   INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER

Cooperative Vaccinia Infection Demonstrated at the Single-Cell Level Using FluidFM

(2012) Philipp Stiefel et al.   NANO LETTERS

Voltage controlled nano-injection system for single-cell surgery

(2012) R. Adam Seger et al.   Nanoscale

Force-Controlled Fluidic Injection into Single Cell Nuclei

(2012) Orane Guillaume-Gentil et al.   Small

A FRET Sensor for Non-Invasive Imaging of Amyloid Formation in Vivo

(2011) Gabriele S. Kaminski Schierle et al.   CHEMPHYSCHEM

Nanostraws for Direct Fluidic Intracellular Access

(2011) Jules J. VanDersarl et al.   NANO LETTERS

Physiological validation of cell health upon probing with carbon nanotube endoscope and its benefit for single-cell interrogation

(2011) Zulfiya Orynbayeva et al.   Nanomedicine-Nanotechnology Biology and Medicine

Mammalian cell transfection: the present and the future

(2010) Tae Kyung Kim et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Multifunctional carbon-nanotube cellular endoscopes

(2010) Riju Singhal et al.   Nature Nanotechnology

Electroosmotic Flow Can Generate Ion Current Rectification in Nano- and Micropores

(2009) Erik C. Yusko et al.   ACS Nano

Mechanisms of Endocytosis

(2009) Gary J. Doherty et al.   Annual Review of Biochemistry

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

(2009) Harold P. Erickson   BIOLOGICAL PROCEDURES ONLINE

Electroporation based on hydrodynamic focusing of microfluidics with low dc voltage

(2009) Tao Zhu et al.   BIOMEDICAL MICRODEVICES

FluidFM: Combining Atomic Force Microscopy and Nanofluidics in a Universal Liquid Delivery System for Single Cell Applications and Beyond

(2009) André Meister et al.   NANO LETTERS