4.8 Article

Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes

期刊

NANO LETTERS
卷 15, 期 2, 页码 1374-1381

出版社

AMER CHEMICAL SOC
DOI: 10.1021/nl504660t

关键词

Nanoinjection; nanopipette; online monitoring of live-cell labeling; live-cell imaging; super-resolution imaging; scanning ion conductance microscopy

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Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of <100 nm, we deliver functionalized fluorescent probes directly into the cells by (di)electrophoretic forces. The label density can be adjusted and traced directly during the staining process by fluorescence microscopy. We demonstrate the potential of this technique by delivering and imaging a range of commercially available cell-permeable and nonpermeable fluorescent probes to cells.

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