Review
Chemistry, Multidisciplinary
Li Chen, Shi-qi Cao, Ze-min Lin, Shi-jun He, Jian-ping Zuo
Summary: Autoimmune diseases are characterized by dysregulation of the immune system, with NLRs playing essential roles in various diseases. NLR proteins recognize specific molecular patterns to regulate immune response pathways.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Biochemistry & Molecular Biology
Yukako Senga, Motomichi Doi, Masayoshi Onitsuka, Shinya Honda
Summary: This study establishes an approach to detect intracellular aggregates using a fluorescent-labeled artificial protein, AF.2A1, and finds that the relative amount of intracellular aggregates is correlated with the type of additives used during cell culture. Additionally, there is a moderate correlation between the relative amount of intracellular aggregates and that of extracellular aggregates, suggesting the secretion of intracellular aggregates.
CELL CHEMICAL BIOLOGY
(2022)
Article
Engineering, Chemical
Simon A. B. Riedl, Valerie Jerome, Ruth Freitag
Summary: This study demonstrates that transient gene expression (TGE)-based production of BMP2 is more efficient in HEKsus cells than in CHOsus cells. The use of a repeated transient transfection (RTT) process in HEKsus cells significantly improves BMP2 production and extends the production phase.
Article
Biotechnology & Applied Microbiology
Simon Umbach, Roman Levin, Sebastian Neumann, Torsten Steinmetzer, Volker Doetsch, Frank Bernhard
Summary: Nanodiscs are a transfer vector for inserting recombinant membrane proteins into living cell membranes. This approach, combined with cell-free expression technologies, allows the analysis of challenging membrane proteins in complex cellular environments. The transfer process is efficient, controllable, and can be applied to different cell lines. Nanodiscs disassemble during transfer, and only lipids and membrane proteins are transferred into cell membranes. The transferred membrane proteins retain their functionality in the cell environment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Katarzyna Wicherska-Pawlowska, Tomasz Wrobel, Justyna Rybka
Summary: The innate immune system recognizes pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) through pattern recognition receptors (PRRs), activating immune cells and initiating inflammatory and immune responses. This system also plays a role in cancer immunosurveillance, with potential for natural and synthetic agonists of PRRs to be used as adjuvants in cancer immunotherapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Juan Jin, Tao-jie Zhou, Gui-ling Ren, Liang Cai, Xiao-ming Meng
Summary: NLRs, a major subfamily of pattern recognition receptors, have gained considerable attention due to their involvement in mediating the immune response and inflammatory pathways. This review summarises recent progress on the functions and mechanisms of NLRs in different types of renal diseases.
ACTA PHARMACOLOGICA SINICA
(2022)
Review
Biochemistry & Molecular Biology
Gang Pei, Anca Dorhoi
Summary: The innate immune system relies on pattern recognition receptors to detect microbial motifs, trigger immune responses, and regulate adaptive immunity. NLRs, in particular, are highlighted for their role in inflammation during cellular stress induced by infection. Understanding cellular homeostasis disruption and NLR functions provides insight into infection mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Immunology
Cassio Luiz Coutinho Almeida-da-Silva, Luiz Eduardo Baggio Savio, Robson Coutinho-Silva, David M. Ojcius
Summary: The innate immune system in vertebrates and invertebrates relies on conserved receptors and ligands, and pathways to initiate a rapid response against microbial infections and other sources of stress and danger. Research on NOD-like receptors (NLRs) has advanced in the past two decades, providing insight into the ligands and conditions that activate NLRs, as well as the outcomes of NLR activation in cells and animals. NLRs play crucial roles in diverse functions, such as transcription of MHC molecules and initiation of inflammation, and their activation can be direct or indirect depending on the ligands involved. Future discoveries will contribute to a better understanding of the molecular details and physiological consequences of NLR activation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Engineering, Chemical
Carina Bannach, Daniel Ruiz Buck, Genna Bobby, Leo P. Graves, Sainan Li, Adam C. Chambers, Elizabeth Gan, Raquel Arinto-Garcia, Robert D. Possee, Linda A. King
Summary: Autographa californica nucleopolyhedrovirus is an efficient expression vector for recombinant proteins in insect cells. The study found that using the AUA codon may slightly improve protein yield, while the AUU codon may lead to leaky translation.
Article
Biotechnology & Applied Microbiology
Christine Faust, Christian Beil, Werner Dittrich, Ercole Rao, Thomas Langer
Summary: The presence of LPS from E. coli has a detrimental impact on cell viability and recombinant protein expression at high concentrations, but low concentrations of LPS can be tolerated and even enhance protein expression levels.
ENGINEERING IN LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shaowei Sui, Hao Wang, Jiajie Song, Wanyi Tai
Summary: Transient expression is the only way to quickly obtain a small scale of antibodies for biomedical research and therapeutic evaluation. A novel spermine-based lipid, sperminoliposome, was developed as an effective and cheap transfection agent for antibody expression. Compared to PEI, sperminoliposome achieved a 3 times higher yield of antibody during transient expression in suspension cells, confirming its feasibility and efficiency for rapid antibody production.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Jordan Joiner, Zhuangrong Huang, Kyle McHugh, Matthew Stebbins, Kathryn Aron, Michael Borys, Anurag Khetan
Summary: Providing rAAV supply for clinical trials and commercial demand remains challenging due to high dosing requirements and low process efficiency. Despite decades of research, key factors in rAAV manufacturing processes are still relatively understudied, leading to low yields and purification burdens. Modeling approaches are essential for bioprocess optimization, and there is a need for future development of hybrid models to understand underlying mechanisms.
CURRENT OPINION IN CHEMICAL ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Jesus Lavado-Garcia, Andy Diaz-Maneh, Nuria Canal-Pauli, Pol Perez-Rubio, Francesc Godia, Laura Cervera
Summary: The study shows that transient metabolic engineering can optimize the production efficiency of HIV-1 Gag virus-like particles (VLPs), including increasing VLP yield and transfection efficiency. Decreasing the inhibition of Gag-binding protein CNP can significantly enhance VLP specific productivity, and combining it with overexpression of UGCG can further improve budding efficiency of VLPs.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Cell Biology
Jack-Christophe Cossec, Tatiana Traboulsi, Sebastien Sart, Yann Loe-Mie, Manuel Guthmann, Ivo A. Hendriks, Ilan Theurillat, Michael L. Nielsen, Maria-Elena Torres-Padilla, Charles N. Baroud, Anne Dejean
Summary: Recent advances in synthetic embryology have revealed that chemical inhibition of SUMOylation in mouse embryonic stem cells (ESCs) can lead to the generation of embryo-like structures. These structures can self-organize into gastrulating structures and undergo axial organization reminiscent of natural embryo morphogenesis. This approach provides a proof of principle for exploring early embryogenesis by targeting chromatin roadblocks of cell fate change.
Article
Fisheries
Yingqin Liu, Xiuzhen Sheng, Xiaoqian Tang, Jing Xing, Heng Chi, Wenbin Zhan
Summary: This study provides a comprehensive analysis of the NLR gene family in flounder, revealing their important roles in fish immunity and highlighting their diverse functions and interactions.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Kirsten J. Koymans, Louris J. Feitsma, T. Harma C. Brondijk, Piet C. Aerts, Eddie Lukkien, Philip Lossl, Kok P. M. van Kessel, Carla J. C. de Haas, Jos A. G. van Strijp, Eric G. Huizinga
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Biochemistry & Molecular Biology
Christoph A. Diebolder, Els F. Halff, Abraham J. Koster, Eric G. Huizinga, Roman I. Koning
Article
Biochemistry & Molecular Biology
Guillermo Lopez-Domenech, Christian Covill-Cooke, Davor Ivankovic, Els F. Halff, David F. Sheehan, Rosalind Norkett, Nicol Birsa, Josef T. Kittler
Article
Multidisciplinary Sciences
Jacco C. Karper, Mark M. Ewing, Margreet R. de Vries, Saskia C. A. de Jager, Erna A. B. Peters, Hetty C. de Boer, Anton-Jan van Zonneveld, Johan Kuiper, Eric G. Huizinga, T. Harma C. Brondijk, J. Wouter Jukema, Paul H. A. Quax
Article
Immunology
Ramon M. van den Bos, Nicholas M. Pearce, Joke Granneman, T. Harma C. Brondijk, Piet Gros, S. Berger, J. van den Born, L. van den Heuvel, N. van de Kar, C. van Kooten, M. Seelen, A. de Vries, P. Gros
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Souvik Modi, Guillermo Lopez-Domenech, Elise F. Halff, Christian Covill-Cooke, Davor Ivankovic, Daniela Melandri, I. Lorena Arancibia-Carcamo, Jemima J. Burden, Alan R. Lowe, Josef T. Kittler
NATURE COMMUNICATIONS
(2019)
Article
Cell Biology
Els F. Halff, Blanka R. Szulc, Flavie Lesept, Josef T. Kittler
Article
Multidisciplinary Sciences
Ellis Chika Onwordi, Els F. Halff, Thomas Whitehurst, Ayla Mansur, Marie-Caroline Cotel, Lisa Wells, Hannah Creeney, David Bonsall, Maria Rogdaki, Ekaterina Shatalina, Tiago Reis Marques, Eugenii A. Rabiner, Roger N. Gunn, Sridhar Natesan, Anthony C. Vernon, Oliver D. Howes
NATURE COMMUNICATIONS
(2020)
Article
Behavioral Sciences
Els F. Halff, Marie-Caroline Cotel, Sridhar Natesan, Richard McQuade, Chris J. Ottley, Deepak P. Srivastava, Oliver D. Howes, Anthony C. Vernon
Summary: PET studies using SV2A radioligand provide evidence for synaptic dysfunction in the cingulate and frontal cortex of schizophrenia patients. Chronic exposure to antipsychotics did not affect SV2A levels, while chronic lithium exposure increased NLGN density. Further research is needed to understand the functional consequences of these changes.
BEHAVIOURAL BRAIN RESEARCH
(2021)
Article
Hematology
Louris J. Feitsma, Harma C. Brondijk, Gavin E. Jarvis, Dominique Hagemans, Dominique Bihan, Natasia Jerah, Marian Versteeg, Richard W. Farndale, Eric G. Huizinga
Summary: Glycoprotein VI (GPVI) plays a crucial role in collagen-induced platelet activation and is essential in the development of thrombosis, heart attack, and stroke. This study reveals the crystal structures of GPVI bound to collagen peptides and identifies the binding site and essential residues for collagen binding. The study also demonstrates the binding of GPVI to collagen chains containing the sequence motif OGPOGP. These findings provide insights for the development of receptor-specific therapeutics targeting GPVI.
Article
Biochemistry & Molecular Biology
Els F. Halff, Saad Hannan, Jaturon Kwanthongdee, Flavie Lesept, Trevor G. Smart, Josef T. Kittler
Summary: A study has found that cAMP-dependent protein kinase (PKA) plays a key role in the synaptic stabilization of neuroligin-2 (NL2). PKA-mediated phosphorylation of NL2 causes its dispersal from the synapse and reduces the amount of synaptic GABA(A)Rs. Enhancing the stability of NL2 at synapses by inhibiting PKA-mediated phosphorylation leads to increased inhibitory signaling.
Article
Biochemical Research Methods
Els F. Halff, Sridhar Natesan, David R. Bonsall, Mattia Veronese, Anna Garcia-Hidalgo, Michelle Kokkinou, Sac-Pham Tang, Laura J. Riggall, Roger N. Gunn, Elaine E. Irvine, Dominic J. Withers, Lisa A. Wells, Oliver D. Howes
Summary: This study evaluated intraperitoneal administration of [F-18]-FDOPA as a less invasive alternative to intravenous administration for longitudinal imaging studies. The results showed that intraperitoneal [F-18]-FDOPA administration resulted in good brain uptake and yielded similar estimates of dopamine synthesis capacity as intravenous administration. The technique supported longitudinal studies and demonstrated the lasting effects of subchronic ketamine on the dopamine system.
Article
Developmental Biology
Hrvoje Augustin, Kieran McGourty, Joern R. Steinert, Helena M. Cocheme, Jennifer Adcott, Melissa Cabecinha, Alec Vincent, Els F. Halff, Josef T. Kittler, Emmanuel Boucrot, Linda Partridge
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)