Article
Biochemistry & Molecular Biology
Ok-Hee Kim, Geun-Hyung Kang, June Hur, Jinwook Lee, YunJae Jung, In-Sun Hong, Hookeun Lee, Seung-Yong Seo, Dae Ho Lee, Cheol Soon Lee, In-Kyu Lee, Susan Bonner-Weir, Jongsoon Lee, Young Joo Park, Hyeonjin Kim, Steven E. Shoelson, Byung-Chul Oh
Summary: Using unbiased proteomics, this study identified externalized phosphatidylinositides as apoptotic eat-me signals recognized by CD14(+) phagocytes.
CELL DEATH AND DIFFERENTIATION
(2022)
Review
Cell Biology
Maria C. Tanzer
Summary: Efferocytosis is the process of phagocytes removing dead or dying cells, which is considered anti-inflammatory. However, it can induce inflammatory signaling pathways under certain conditions. The mechanisms and molecules involved in this response are not well understood. This article discusses the influence of dead cell cargo, ingestion type, and digestion efficiency on phagocyte programming in disease contexts, presents recent findings, identifies knowledge gaps, and proposes experimental approaches to fill them.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Multidisciplinary Sciences
Amirreza Khalaji, Fatereh Baharlouei Yancheshmeh, Fatemeh Farham, Arya Khorram, Shiva Sheshbolouki, Maryam Zokaei, Fatemeh Vatankhah, Mehdi Soleymani-Goloujeh
Summary: Cancer is a major cause of death globally, and there is a need for new immunology-based strategies to combat it. The identification of "eat me" and "Don't eat me" signals in cancer cells holds promise for developing new treatment approaches.
Review
Biochemistry & Molecular Biology
Gabriel Ichim, Benjamin Gibert, Sahil Adriouch, Catherine Brenner, Nathalie Davoust, Solange Desagher, David Devos, Svetlana Dokudovskaya, Laurence Dubrez, Jerome Estaquier, Germain Gillet, Isabelle Guenal, Philippe P. Juin, Guido Kroemer, Patrick Legembre, Romain Levayer, Stephen Manon, Patrick Mehlen, Olivier Meurette, Olivier Micheau, Bernar Mignotte, Florence Nguyen-Khac, Nikolay Popgeorgiev, Jean-Lu Poyet, Muriel Priault, Jean-Ehrlan Ricci, Franck B. Riquet, Santos A. Susin, Magal Suzanne, Pierre Vacher, Ludivine Walter, Bertran Mollereau
Summary: Since the Nobel Prize was awarded more than twenty years ago for discovering the core apoptotic pathway in C. elegans, researchers around the world have conducted extensive research on apoptosis and various other forms of regulated cell death. Although there are still many aspects of regulated cell death that need to be clarified in specific cell subtypes and disease conditions, the last decade has seen the description of multiple cell death modalities, some of which have been successfully used in clinical therapy. To keep research into cell death alive, francophone researchers from several institutions in France and Belgium established the French Cell Death Research Network (FCDRN), which is at the forefront of emerging topics in cell death research. These research efforts will enhance our mechanistic knowledge of regulated cell death and its therapeutic applications in the coming years.
Article
Biochemistry & Molecular Biology
Sergio M. Pontejo, Philip M. Murphy
Summary: Phagocytes are attracted to apoptotic cells through the binding of chemokines to PS on the cell surface; many chemokines have a high affinity for PS and other anionic phospholipids; apoptotic cells down-regulate GAGs and up-regulate PS, allowing PS-bound chemokines to directly activate chemokine receptors on leukocytes.
Article
Multidisciplinary Sciences
Bartosz Wiernicki, Sophia Maschalidi, Jonathan Pinney, Sandy Adjemian, Tom Vanden Berghe, Kodi S. Ravichandran, Peter Vandenabeele
Summary: Ferroptosis, a form of cell death characterized by iron accumulation and lipid peroxidation, has lower immunogenicity compared to other cell death modalities, inhibiting the maturation and antigen cross-presentation of immune cells and hence weakening the anti-tumor immune response.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Shannon M. Kelley, Kodi S. Ravichandran
Summary: Phagocytosis plays a crucial role in maintaining host homeostasis by timely clearing dying or pathogenic cells, regulated by a balance of eat-me and don't-eat-me signals. Eat-me signals activate pro-phagocytic receptors to promote phagocytosis, while don't-eat-me signals engage anti-phagocytic receptors to suppress phagocytosis. Aberrant don't-eat-me signaling is associated with pathology in normal physiology and disease contexts.
Review
Immunology
Yong Xi, Lijie Chen, Jian Tang, Bentong Yu, Weiyu Shen, Xing Niu
Summary: Immunogenic cell death (ICD) is a unique mode of cell death that triggers long-term protective antitumor immune responses by releasing immunogenic damage-associated molecular patterns (DAMPs) and tumor-associated antigens. Amplifying the "eat me" signal during the tumor ICD cascade is critical for cancer immunotherapy.
IMMUNOLOGICAL REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Leon Mendel McClusky
Summary: The process of spermatogenesis involves apoptosis and germ cell loss. Research on the testes of migrating elasmobranchs has provided insights into the apoptotic process.
Article
Medicine, Research & Experimental
Hend Mohamed Abdel-Bar, Adam A. Walters, Yau Lim, Nadia Rouatbi, Yue Qin, Fatemeh Gheidari, Shunping Han, Rihab Osman, Julie Tzu-Wen Wang, Khuloud T. Al-Jamal
Summary: The study developed a stable nucleic acid-lipid particles (SNALPs) formulation for the simultaneous delivery of ICD inducing drug and siRNA targeting CD47 for enhanced ICD. The results demonstrated the potential of this combination as a potent anti-tumor strategy for future clinical studies.
Review
Oncology
Jaime Huertas, H. Thomas Lee
Summary: This review summarizes the diverse physiological effects of cathepsins, including immune responses, cell death, and intracellular protein degradation. Cathepsins play a crucial role in ischemia reperfusion injury, and modulation of cathepsins may have therapeutic potential for attenuating such injuries.
MOLECULAR MEDICINE REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Yuxi Zhang, Zhenghai Zhang, Senlin Li, Liang Zhao, Dongdong Li, Ziyang Cao, Xiaoding Xu, Xianzhu Yang
Summary: The study utilized a siRNA-assisted assembly strategy to deliver siRNA and MTO simultaneously, effectively enhancing phagocytosis of tumor cells by macrophages. This approach promoted antigen presentation and triggered T cell-mediated immune responses, leading to significantly improved antitumor activity in aggressive tumor animal models.
Article
Materials Science, Biomaterials
Guibin Pang, Siqi Wei, Jian Zhao, Fu-Jun Wang
Summary: In this study, a strategy combining immunogenic cell death (ICD) effects with CD47/SIRP alpha blockade was proposed to enhance macrophage phagocytosis of tumor cells during cancer treatment. A novel CD47 modulator, gefitinib, was identified and used in combination with immunostimulatory polysaccharides from Ganoderma lucidum. The approach downregulated CD47 expression, increased drug accumulation in tumors, and induced immune cell activation, leading to suppressed tumor dissemination and improved therapeutic outcomes.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Biochemistry & Molecular Biology
Tapan Behl, Rashita Makkar, Aayush Sehgal, Sukhbir Singh, Neelam Sharma, Gokhan Zengin, Simona Bungau, Felicia Liana Andronie-Cioara, Mihai Alexandru Munteanu, Mihaela Cristina Brisc, Diana Uivarosan, Ciprian Brisc
Summary: The human body is complex and comprises various living cells and extracellular material forming tissues, organs, and organ systems. While most human cells turnover regularly, postmitotic nerve cells have the exceptional ability to regenerate and sustain throughout an individual's life to safeguard the central nervous system's functioning. Neurodegenerative diseases, such as Parkinson's and Alzheimer's disease, result from factors like oxidative stress, cell death, and neuroinflammation, causing neuronal degradation and impacting the quality of life for millions worldwide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Huyan Meng, Guowei Wu, Xinsuo Zhao, Anhui Wang, Dekang Li, Yilun Tong, Taijie Jin, Ye Cao, Bing Shan, Shichen Hu, Ying Li, Lifeng Pan, Xiaoxu Tian, Ping Wu, Chao Peng, Junying Yuan, Guohui Li, Li Tan, Zhaoyin Wang, Ying Li
Summary: Necrostatin-34 (Nec-34), a small molecule inhibitor of RIPK1 kinase, stabilizes RIPK1 in an inactive conformation by occupying a distinct binding pocket. It can synergize with Nec-1s to inhibit RIPK1 in vitro and in vivo, providing a new strategy for targeting RIPK1 kinase and potential option for combinatorial therapy for RIPK1-mediated diseases.
Article
Microbiology
Johannes Westman, Glenn F. W. Walpole, Lydia Kasper, Bessie Y. Xue, Osama Elshafee, Bernhard Hube, Sergio Grinstein
CELL HOST & MICROBE
(2020)
Review
Microbiology
Sophie Austermeier, Lydia Kasper, Johannes Westman, Mark S. Gresnigt
CURRENT OPINION IN MICROBIOLOGY
(2020)
Review
Cell Biology
Sofie Vandendriessche, Seppe Cambier, Paul Proost, Pedro E. Marques
Summary: The complement system plays a crucial role in controlling microorganisms and removing dead cells, while also stimulating adaptive immune responses and tissue regeneration. Complement receptors on leukocytes and parenchymal cells are essential for recognizing complement-derived molecules and promoting immune responses.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Marfa Blanter, Seppe Cambier, Mirre De Bondt, Lotte Vanbrabant, Noemie Poertner, Sara Abouelasrar Salama, Mieke Metzemaekers, Pedro Elias Marques, Sofie Struyf, Paul Proost, Mieke Gouwy
Summary: This study compares the effects of classic density-gradient purification and immunomagnetic isolation on the phenotype and functionality of neutrophils. The results show that the two methods produce cells with different characteristics under different experimental conditions. Density-gradient-derived neutrophils exhibit increased polarization responses and release of reactive oxygen species, neutrophil extracellular traps, and granular proteins, while immunomagnetic isolation-derived neutrophils are mostly quiescent. The choice of purification method should depend on the specific experimental purposes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Daiane Boff, Remo Castro Russo, Helena Crijns, Vivian Louise Soares de Oliveira, Matheus Silverio Mattos, Pedro Elias Marques, Gustavo Batista Menezes, Angelica Thomaz Vieira, Mauro Martins Teixeira, Paul Proost, Flavio Almeida Amaral
Summary: This study tested the anti-inflammatory effect of CXCL9(74-103) in Klebsiella pneumoniae-induced pneumonia. The treatment with CXCL9(74-103) reduced neutrophil migration, decreased the production of IL-1 beta, and improved lung function in infected mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Thiago Henrique Caldeira Oliveira, Vincent Vanheule, Sofie Vandendriessche, Fariba Poosti, Mauro Martins Teixeira, Paul Proost, Mieke Gouwy, Pedro Elias Marques
Summary: The study suggests that interfering with chemokine function using a peptide called MIG30 can disrupt neutrophil recruitment to the injured liver during ischemia-reperfusion injury (IRI). Mice treated with MIG30 showed significantly lower liver injury and dysfunction, as well as reduced levels of chemokines and proinflammatory cytokines. This indicates that MIG30 holds promise as a potential treatment strategy for liver IRI and inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lisa Verhallen, Jarkko J. J. Lackman, Rikke Wendt, Martin Gustavsson, Zhang Yang, Yoshiki Narimatsu, Daniel M. M. Sorensen, Kato Mac Lafferty, Mieke Gouwy, Pedro E. E. Marques, Gertrud M. M. Hjorto, Mette M. M. Rosenkilde, Paul Proost, Christoffer K. K. Goth
Summary: Chemokine ligands and receptors play a role in regulating leukocyte migration. This study examined the post-translational modifications of chemokine receptors and their impact on ligand binding and signaling. The results indicated that O-glycosylation and tyrosine sulfation affect the recognition and signaling of chemokines with the receptor CCR5. The enzymes GalNAc-T1 and GalNAc-T11 were identified as being involved in the O-glycosylation process of chemokine receptors.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Editorial Material
Immunology
A. Baragetti, J. Suurmond, P. E. Marques, L. P. Tavares
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Mycology
Caio Ambrosio Leal-Dutra, Lok Man Yuen, Bruno Augusto Maciel Guedes, Marta Contreras-Serrano, Pedro Elias Marques, Jonathan Zvi Shik
Summary: Leafcutter ants farm a fungal cultivar called Leucoagaricus gongylophorus, which forms gongylidia cells through autophagy, providing nutritional rewards for ant farmers. This study used microscopy and experimental analysis to uncover the mechanisms of gongylidia formation and found that autophagy plays a critical role in this process.
Meeting Abstract
Gastroenterology & Hepatology
Matheus Mattos, Sofie Vandendriessche, Sara Schuermans, Romy Mittenzwei, Ari Waisman, Pedro Elias Marques
JOURNAL OF HEPATOLOGY
(2022)
Proceedings Paper
Computer Science, Information Systems
Pedro Marques, Luis Dias, Miguel Correia
Summary: CYBERVTI is an intrusion detection tool with an effective graphical interface, aiming to assist human analysts in observing threats and studying algorithm performance. It integrates intrusion detection algorithms based on unsupervised machine learning and uses a clear and minimalist GUI.
2021 IEEE 20TH INTERNATIONAL SYMPOSIUM ON NETWORK COMPUTING AND APPLICATIONS (NCA)
(2021)
Article
Gastroenterology & Hepatology
Pedro E. Marques, Sofie Vandendriessche, Thiago H. C. de Oliveira, Helena Crijns, Mateus E. Lopes, Marfa Blanter, Sara Schuermans, Karen Yu, Fariba Poosti, Vincent Vanheule, Rik Janssens, Daiane Boff, Andreas J. Kungl, Gustavo B. Menezes, Mauro M. Teixeira, Paul Proost
Summary: The study investigates the use of DNA-binding peptides in treating drug-induced liver injury, finding that MIG30 can effectively reduce tissue inflammation caused by DNA, decrease mortality and liver damage.
HEPATOLOGY COMMUNICATIONS
(2021)
Proceedings Paper
Computer Science, Software Engineering
Pedro Marques, Murilo Silva, Camila Gusmao, Diego Castro, Marcelo Schots
2020 IEEE 32ND CONFERENCE ON SOFTWARE ENGINEERING EDUCATION AND TRAINING (CSEE&T)
(2020)
Article
Immunology
Mieke Metzemaekers, Seppe Cambier, Marfa Blanter, Jennifer Vandooren, Ana Carolina de Carvalho, Bert Malengier-Devlies, Lore Vanderbeke, Cato Jacobs, Sofie Coenen, Erik Martens, Noemie Portner, Lotte Vanbrabant, Pierre Van Mol, Yannick Van Herck, Nathalie Van Aerde, Greet Hermans, Jan Gunst, Alexandre Borin, Bruna Toledo N. Pereira, Arilson Bernardo dos S. P. Gomes, Stefanie Primon Muraro, Gabriela Fabiano de Souza, Alessandro S. Farias, Jose Luiz Proenca-Modena, Marco Aurelio R. Vinolo, Contagious Consortium, Pedro Elias Marques, Carine Wouters, Els Wauters, Sofie Struyf, Patrick Matthys, Ghislain Opdenakker, Rafael Elias Marques, Joost Wauters, Mieke Gouwy, Paul Proost
Summary: Our study explores the characteristics of neutrophils in severe COVID-19 patients, showing increased plasma concentrations of G-CSF and CXCL8 upon ICU admission, indicating emergency myelopoiesis. COVID-19 neutrophils were hyper-responsive to CXCL8 and CXCL12, suggesting an increased activation state in the circulation.
CLINICAL & TRANSLATIONAL IMMUNOLOGY
(2021)