Article
Chemistry, Multidisciplinary
Jae-Sang Hong, Taehwang Son, Cesar M. M. Castro, Hyungsoon Im
Summary: The authors present an amplification-free and extraction-free method for detecting extracellular vesicles (EVs) miRNA using a CRISPR/Cas13a sensing system. They encapsulate CRISPR/Cas13a sensing components in liposomes and deliver them into EVs through liposome-EV fusion. By quantifying specific miRNA-positive EV counts in 1 x 10(8) EVs, they demonstrate that miR-21-5p-positive EV counts are significantly higher in ovarian cancer EVs compared to benign cells. They also show the potential for multiplexed protein-miRNA analysis in tumor-derived EVs using this method.
Article
Biophysics
Liangfen Cheng, Fuhan Yang, Yining Zhao, Ziye Liu, Xudong Yao, Juan Zhang
Summary: This study utilizes a tetrahedron supported CRISPR/Cas13a cleavage method for direct electrochemical detection of circular RNA (circRNA) in urine from bladder cancer patients. The CRISPR/Cas13a system is designed to recognize the characteristic back-splice junction site of circRNA. The activated CRISPR/Cas13a cleaves uracil bases in a DNA tetrahedron immobilized on a gold electrode, resulting in the release of an electrochemical active molecule, methylene blue. The developed electrochemical biosensor demonstrates high catalytic efficiency and a rigid structure, allowing for direct detection of circRNA in 25 μL urine samples with a lowest detection limit of 0.089 fM and a linear detection range from 10 fM to 50 nM in less than 10 minutes, avoiding the need for complicated extraction and enabling on-site detection.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Chemistry, Multidisciplinary
Ke Yi, Huimin Kong, Yeh-Hsing Lao, Di Li, Rachel L. Mintz, Tianxu Fang, Guojun Chen, Yu Tao, Mingqiang Li, Jianxun Ding
Summary: CRISPR/Cas9 gene-editing technology has great potential in cancer treatment, but limitations of viral delivery have led to the development of non-viral nanomaterials. Optimizing the packaging capacity, pharmacokinetics, and targetability of non-viral CRISPR/Cas9 nanoformulations can enhance the safety and efficacy of cancer therapy.
ADVANCED MATERIALS
(2023)
Review
Pharmacology & Pharmacy
Ning Ding, Sangsin Lee, Matan Lieber-Kotz, Jie Yang, Xue Gao
Summary: With over 466 million people worldwide affected by hearing loss, genetic factors contributing to 50% of congenital hearing loss, CRISPR-Cas9 systems offer new gene therapy strategies for treating genetic hearing impairment. This technology shows promise in targeted gene editing for deafness-associated genes and has the potential to revolutionize the treatment of genetic hearing diseases.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Review
Medicine, Research & Experimental
Asma Ghaemi, Elnaz Bagheri, Khalil Abnous, Seyed Mohammad Taghdisi, Mohammad Ramezani, Mona Alibolandi
Summary: The prokaryotic CRISPR-Cas systems serve as a revolutionary genome editing tool for modifying, visualizing, and identifying DNA and RNA sequences in live cells of various species. Besides genome editing, the DNA-targeting modifications by CRISPR-Cas offer opportunities for diagnosis, therapy, and genetic disorders investigation. Preclinical studies and CRISPR-Cas9-based therapeutics may encounter challenges, but there is potential for its development in cancer therapy.
Review
Biochemistry & Molecular Biology
Si-Wei Wang, Chao Gao, Yi-Min Zheng, Li Yi, Jia-Cheng Lu, Xiao-Yong Huang, Jia-Bin Cai, Peng-Fei Zhang, Yue-Hong Cui, Ai-Wu Ke
Summary: The CRISPR/Cas9 gene editing technology has made significant progress in oncological research, providing a powerful tool for exploring the mechanism of cancer, establishing tumor models, and studying drug targets.
Review
Biochemistry & Molecular Biology
Yu-Fan Chuang, Andrew J. Phipps, Fan-Li Lin, Valerie Hecht, Alex W. Hewitt, Peng-Yuan Wang, Guei-Sheung Liu
Summary: The CRISPR/Cas system is a groundbreaking genetic technology that allows gene modification by targeting specific genomic sites. Despite its versatility, the translation of CRISPR/Cas system still faces hurdles that need to be overcome, especially in improving delivery efficiency for potential applications in various fields.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Xing Yin, Romain Harmancey, David D. McPherson, Hyunggun Kim, Shao-Ling Huang
Summary: The efficient delivery of CRISPR/Cas remains a challenge in gene editing, but liposomes provide a promising approach to enhance the delivery of CRISPR/Cas and guide RNA. Liposomes facilitate endosomal escape and offer spatial and temporal control of cargo release, making them an effective tool for precise and efficient genetic modifications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Zihao Song, Yue Yu, Xinpeng Bai, Yiguo Jia, Jiayi Tian, Kui Gu, Mengyu Zhao, Changyu Zhou, Xiangyu Zhang, Hongning Wang, Yizhi Tang
Summary: The emergence of antibiotic-resistant bacteria poses a threat to public health. The use of broad-spectrum antibiotics can disrupt beneficial gut microbiota and lead to secondary diseases. Therefore, there is a need to develop a new strategy that can specifically kill pathogens without affecting the resident microbiota. In this study, researchers developed a precise bactericidal system using CRISPR-Cas13a targeting endogenous transcripts of Salmonella enterica serovar Typhimurium. The system demonstrated specific killing, growth inhibition, and clearance of S. Typhimurium in mixed microbial flora in vitro. In a mouse infection model, the system significantly reduced S. Typhimurium colonization in the intestinal tract when delivered by a donor Escherichia coli strain. These findings demonstrate the feasibility and efficacy of the designed CRISPR-Cas13a system in selective killing of pathogens and broaden the utility of conjugation-based delivery of bactericidal approaches.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Physical
Juhee Lee, Yoo Kyung Kang, Eonju Oh, Juhee Jeong, San Hae Im, Duk Ki Kim, Haeshin Lee, Sang-Gyu Kim, Keehoon Jung, Hyun Jung Chung
Summary: The study presents a cancer gene therapy strategy based on NanoRNP that efficiently blocks the PD-L1 immune checkpoint and induces an antitumor effect in vivo without the need for combination therapy. In vivo results demonstrate that NanoRNP can induce indels in target cells at high frequencies, significantly suppressing tumor growth.
CHEMISTRY OF MATERIALS
(2022)
Article
Biophysics
Yan Sheng, Tenghua Zhang, Shihong Zhang, Midori Johnston, Xiaohe Zheng, Yuanyue Shan, Tong Liu, Zena Huang, Feiyang Qian, Zihui Xie, Yiru Ai, Hankang Zhong, Tairong Kuang, Can Dincer, Gerald Anton Urban, Jiaming Hu
Summary: The study introduces a dual signal amplification strategy using the CRISPR/Cas13a system and CHDC for rapid and accurate detection of low expression level RNAs in early-stage cancer. The reusable biosensor platform showed high specificity and sensitivity in distinguishing early-stage lung cancer patients from healthy subjects.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Medicine, Research & Experimental
Chao Liu, Ning Wang, Rui Luo, Lu Li, Wen Yang, Xiye Wang, Meiling Shen, Qinjie Wu, Changyang Gong
Summary: The study presented a novel nanoCRISPR, named PICASSO, that efficiently activates tumor endogenous genes for effective anti-tumor therapy. By targeting endogenous genes in tumor cells, the therapy can inhibit tumor growth effectively, overcoming the limitations of gene size and type.
Review
Pharmacology & Pharmacy
Mujeeb Ur Rahman, Muhammad Bilal, Junaid Ali Shah, Ajeet Kaushik, Pierre-Louis Teissedre, Malgorzata Kujawska
Summary: Parkinson's disease and other neurodegenerative diseases have a significant impact on individuals and society. Recent research has identified neuroinflammation, oxidative stress, dysregulated protein degradation, and mitochondrial dysfunction as primary causes of Parkinson's disease. Gene-editing technologies hold promise for new treatment options.
Article
Engineering, Biomedical
Lin Liu, Zheyu Wang, Yixuan Wang, Jingyi Luan, Jeremiah J. Morrissey, Rajesh R. Naik, Srikanth Singamaneni
Summary: This study demonstrates a novel, ultrasensitive, and amplification-free RNA quantification method by integrating CRISPR-Cas13a system with a plasmonic nanolabel. The method shows great potential in quantitative monitoring of cancer biomarkers and provides a rapid, sensitive strategy for detection of various RNA biomarkers, even in resource-limited settings.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Saeid Moghassemi, Arezoo Dadashzadeh, Ricardo Bentes Azevedo, Olivier Feron, Christiani A. Amorim
Summary: Photodynamic therapy (PDT) combines photochemistry and photophysics sciences for cancer therapy, showing significant potential. Despite advantages and progress, common photosensitizing agents have limitations, which can be addressed by using liposomes as carriers for PS.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biotechnology & Applied Microbiology
Zhao J. Lyu, Ying Wang, Jin L. Huang, Miao Chen, Sha Y. Wu, Qian Yan, Yu Zhang, Ying Tang, Chen Jiang, Lei Li, Yi Z. Jia, Yu C. Liu, Hong B. Mei, Feng Wang, Ren H. Li, Ya C. Chen, Xiang Lin, Zhi M. Cai, Xin Y. Guan
Summary: Urothelial carcinoma (UC) patients exhibit a significant prevalence (18%) of recurrent somatic mutations in the zinc finger protein gene ZNF83, with a hotspot mutation, p.E293V, showing correlation with poor prognosis and increased tumor growth. This mutation activates the NF-kappa B pathway, leading to enhanced tumor growth and decreased apoptosis. Inhibitors of NF-kappa B have been shown to pharmacologically block tumor growth in mice engrafted with UC cells expressing ZNF83-E293V, suggesting a potential therapeutic strategy for UC.
Article
Obstetrics & Gynecology
Depeng Zhao, Guanglin Zhao, Jing Fan, Haiyan Chen, Enrico Lopriore, Xuemei Li
Summary: The study found that the live birth rate in twin pregnancies after FET is comparable between modified natural cycle (mNC) and ovulation induction (OI) regimens for endometrium preparation.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2021)
Article
Obstetrics & Gynecology
Depeng Zhao, Jing Fan, Ping Wang, Xuan Jiang, Jilong Yao, Xuemei Li
Summary: The study aimed to define an age-specific reference for low AMH and evaluate the outcomes of IVF treatment in women. Results showed that women with AMH levels above the 10th centile had better outcomes in ovarian stimulation, with significantly higher clinical pregnancy and ongoing pregnancy rates in the subgroup with AMH levels above the 10th centile compared to those with low AMH levels in patients under 35 years old.
BMC PREGNANCY AND CHILDBIRTH
(2021)
Article
Obstetrics & Gynecology
Jiao Luo, Yan Yang, Xunmin Ji, Weiyi He, Jing Fan, Yadong Huang, Yulong Wang
Summary: The study demonstrates that nasal delivery of NGF can improve spermatogenesis in azoospermic mice by activating the HPG axis and increasing androgenic hormones.
REPRODUCTIVE SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Jing Fan, Yangyang Sun, Jiao Luo, Depeng Zhao, Xuemei Li
Summary: The study introduces artificial mineralization operations on human oocytes, resulting in the formation of a calcium phosphate-derivative mineralized shell on the zona pellucida surface. This biomineralization may offer novel therapeutics for improving embryonic development and implantation potential in the future.
MATERIALS LETTERS-X
(2021)
Article
Engineering, Biomedical
Yiqun Du, Tiantian Song, Jie Wu, Xiao-Dong Gao, Guanghui Ma, Yuchen Liu, Yufei Xia
Summary: This study developed mannosylated Pickering emulsions for targeted delivery of multiple effective components in cancer vaccines. The emulsion increased cellular targeting, cellular uptake, and activation, resulting in enhanced immune activation and potent anti-tumor effects. The strategic loading of components in the emulsion could serve as a targeted delivery platform for enhanced cancer vaccines.
Article
Chemistry, Multidisciplinary
Yiqun Du, Hongzhong Zhou, Ganglin Su, Mengdan Ma, Yuchen Liu
Summary: This study developed a protein corona-driven nanovaccine that effectively resists protein corona-induced antigen extracellular release and promotes antigen cytosolic release under reductive conditions. The nanovaccine demonstrated significant prophylactic and therapeutic antitumor response in a mouse model.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Nanoscience & Nanotechnology
Jing Fan, Dan Wu, Depeng Zhao, Rui Hu, Zhihong Fu, Xuemei Li
Summary: In this study, a novel small molecule drug called Guanidoestradiol (GDT) was developed for targeting the female reproductive system and releasing nitric oxide. The results showed that GDT effectively increased the concentration of nitric oxide in the blood and had beneficial effects on the reproductive system.
SCIENCE OF ADVANCED MATERIALS
(2022)
Article
Cell Biology
Yonghao Zhan, Aolin Li, Congcong Cao, Yuchen Liu
Summary: In this study, the CRISPR signal conductor 2.0 was developed by integrating various lncRNA functional motifs into the CRISPR-dCas phi system. This system can directly regulate target gene expression and efficiently sense protein signals undetected by classical synthetic systems.
Article
Oncology
Chaojie Xu, Lishan Song, Yubin Yang, Yi Liu, Dongchen Pei, Jiabang Liu, Jianhua Guo, Nan Liu, Xiaoyong Li, Yuchen Liu, Xuesong Li, Lin Yao, Zhengjun Kang
Summary: This study comprehensively analyzed the role of M2 macrophages in tumor progression, identified two genes strongly correlated with LUAD prognosis, and constructed a prognostic nomogram with good performance. HRG patients were suitable for new ICI immunotherapy, while LRG was more suitable for CTLA4-immunosuppressive therapy alone.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Aolin Li, Congcong Cao, Ying Gan, Xiaofei Wang, Tianyu Wu, Quan Zhang, Yuchen Liu, Lin Yao, Qian Zhang
Summary: ZNF677 functions as a tumor suppressor in RCC and its low expression is associated with unfavorable prognosis and decreased m(6)A modification level. Furthermore, m(6)A modification regulates the translation and mRNA stability of ZNF677.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Xinbo Huang, Mingxia Wang, Xia Wu, Yanfen Zou, Jinming Xu, Congcong Cao, Qian Ma, Bo Yu, Yuchen Liu, Yaoting Gui
Summary: In this study, a group of single-stranded DNA aptamers with high affinity and high specificity for the Cas9 protein were obtained. The aptamers were found to effectively regulate dCas9-modulated gene transcription and improve the efficiency of gene homologous recombination repair mediated by CRISPR-Cas9. These findings suggest a potential method to better control precise gene editing and enrich the diversity of modulating tools for the CRISPR-Cas9 system.
Article
Biochemistry & Molecular Biology
Congcong Cao, Aolin Li, Chaojie Xu, Baorui Wu, Jun Liu, Yuchen Liu
Summary: The CRISPR/dCasRx-SINEB2 technology has been developed to target translation of mRNAs using lncRNA functional elements. It has shown effective and adaptable results in increasing the expression of various proteins with minimal off-target effects. Additionally, it can prevent cancer cell proliferation and migration by targeting mRNA translation of antitumor proteins. This study provides an innovative protein enhancement method with multiple applications in biopharmaceutical production and cancer research.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Yonghao Zhan, Congcong Cao, Aolin Li, Hongbing Mei, Yuchen Liu
Summary: This study demonstrates that the fusion of CasRx crRNA with an antisense ribozyme to construct a synthetic fusion guide RNA (CCRS) is more efficient for RNA knockdown and cancer gene therapy than conventional methods.
Article
Medicine, Research & Experimental
Hongzhong Zhou, Yiqun Du, Xiafei Wei, Chunli Song, Jianning Song, Nanson Xu, Weihong Huang, Lichan Chen, Fuwen Yao, Duanming Du, Chuanghua Qiu, Lihong Zhong, Yuchen Liu, Dayong Gu, Jin Wang, Yong Xu
Summary: This study found that DDX56 is upregulated in hepatocellular carcinoma (HCC) tissues and is strongly correlated with poor prognoses for HCC patients. DDX56 promotes HCC cell proliferation by interacting with MECOM to mono-methylate H3K9 on the MIST1 promoter. Furthermore, ZEB1 binds to DDX56 and transcriptionally activates DDX56, leading to HCC tumorigenesis.
