Developments, applications, and prospects of cryo‐electron microscopy

Recognition of the amyloid precursor protein by human γ-secretase

(2019) Rui Zhou et al.   SCIENCE

Mechanism of DNA translocation underlying chromatin remodelling by Snf2

(2019) Meijing Li et al.   NATURE

Cryo-EM structures of herpes simplex virus type 1 portal vertex and packaged genome

(2019) Yun-Tao Liu et al.   NATURE

Late steps in bacterial translation initiation visualized using time-resolved cryo-EM

(2019) Sandip Kaledhonkar et al.   NATURE

Reconstitution and structure of a plant NLR resistosome conferring immunity

(2019) Jizong Wang et al.   SCIENCE

Ligand-triggered allosteric ADP release primes a plant NLR complex

(2019) Jizong Wang et al.   SCIENCE

Molecular Basis for Ligand Modulation of a Mammalian Voltage-Gated Ca2+ Channel

(2019) Yanyu Zhao et al.   CELL

Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome

(2019) Humayun Sharif et al.   NATURE

Cryo-EM structure of the mammalian ATP synthase tetramer bound with inhibitory protein IF1

(2019) Jinke Gu et al.   SCIENCE

The pigment-protein network of a diatom photosystem II–light-harvesting antenna supercomplex

(2019) Xiong Pi et al.   SCIENCE

Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

(2019) Xiao Fan et al.   Nature Communications

Structural basis of assembly of the human T cell receptor–CD3 complex

(2019) De Dong et al.   NATURE

Structural and mechanistic elucidation of inflammasome signaling by cryo-EM

(2019) Chen Shen et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Architecture of African swine fever virus and implications for viral assembly

(2019) Nan Wang et al.   SCIENCE

Cryo-EM in drug discovery: achievements, limitations and prospects

(2018) Jean-Paul Renaud et al.   NATURE REVIEWS DRUG DISCOVERY

Cryo-EM structure of a herpesvirus capsid at 3.1 Å

(2018) Shuai Yuan et al.   SCIENCE

Structure of a human catalytic step I spliceosome

(2018) Xiechao Zhan et al.   SCIENCE

Single-particle cryo-EM—How did it get here and where will it go

(2018) Yifan Cheng   SCIENCE

Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2

(2018) Wenfei Song et al.   PLoS Pathogens

Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome

(2018) Yuanchen Dong et al.   NATURE

Structural basis of Notch recognition by human γ-secretase

(2018) Guanghui Yang et al.   NATURE

Cryo-electron tomography-the cell biology that came in from the cold

(2017) Jonathan Wagner et al.   FEBS LETTERS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Advances in the field of single-particle cryo-electron microscopy over the last decade

(2017) Joachim Frank   Nature Protocols

The structure of the yeast mitochondrial ribosome

(2017) Nirupa Desai et al.   SCIENCE

Structure and assembly mechanism of plant C2S2M2-type PSII-LHCII supercomplex

(2017) Xiaodong Su et al.   SCIENCE

Cryo-EM structure of haemoglobin at 3.2 Å determined with the Volta phase plate

(2017) Maryam Khoshouei et al.   Nature Communications

Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains

(2017) Yuan Yuan et al.   Nature Communications

Structural Insights into the Niemann-Pick C1 (NPC1)-Mediated Cholesterol Transfer and Ebola Infection

(2016) Xin Gong et al.   CELL

Breaking Cryo-EM Resolution Barriers to Facilitate Drug Discovery

(2016) Alan Merk et al.   CELL

High resolution single particle refinement in EMAN2.1

(2016) James M. Bell et al.   METHODS

Structure of spinach photosystem II–LHCII supercomplex at 3.2 Å resolution

(2016) Xuepeng Wei et al.   NATURE

Structure of the voltage-gated calcium channel Cav1.1 at 3.6 Å resolution

(2016) Jianping Wu et al.   NATURE

The architecture of the mammalian respirasome

(2016) Jinke Gu et al.   NATURE

An atomic structure of the human 26S proteasome

(2016) Xiuliang Huang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure and assembly model for theTrypanosoma cruzi60S ribosomal subunit

(2016) Zheng Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the human 26S proteasome at a resolution of 3.9 Å

(2016) Andreas Schweitzer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The 3.8 A structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis

(2016) R. Wan et al.   SCIENCE

Structure of a yeast catalytic step I spliceosome at 3.4 A resolution

(2016) R. Wan et al.   SCIENCE

Structure of a yeast step II catalytically activated spliceosome

(2016) Chuangye Yan et al.   SCIENCE

Architecture of the yeast small subunit processome

(2016) Malik Chaker-Margot et al.   SCIENCE

Key Intermediates in Ribosome Recycling Visualized by Time-Resolved Cryoelectron Microscopy

(2016) Ziao Fu et al.   STRUCTURE

An atomic structure of the human 26S proteasome

(2016) Xiuliang Huang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

(2016) Dari Kimanius et al.   eLife

Structure of the E. coli ribosome–EF-Tu complex at

(2015) Niels Fischer et al.   NATURE

Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization

(2015) L. Zhang et al.   SCIENCE

2.2 A resolution cryo-EM structure of  -galactosidase in complex with a cell-permeant inhibitor

(2015) A. Bartesaghi et al.   SCIENCE

The structure of the human mitochondrial ribosome

(2015) A. Amunts et al.   SCIENCE

Structural and biochemical basis for induced self-propagation of NLRC4

(2015) Z. Hu et al.   SCIENCE

Structural Dynamics of Ribosome Subunit Association Studied by Mixing-Spraying Time-Resolved Cryogenic Electron Microscopy

(2015) Bo Chen et al.   STRUCTURE

2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

(2015) Melody G Campbell et al.   eLife

Single-particle cryo-EM data acquisition by using direct electron detection camera

(2015) Shenping Wu et al.   Microscopy

Structure of the Mammalian Ribosome-Sec61 Complex to 3.4 Å Resolution

(2014) Rebecca M. Voorhees et al.   CELL

The complete structure of the large subunit of the mammalian mitochondrial ribosome

(2014) Basil J. Greber et al.   NATURE

Cryo-EM Study of the Chromatin Fiber Reveals a Double Helix Twisted by Tetranucleosomal Units

(2014) F. Song et al.   SCIENCE

Structure of the Yeast Mitochondrial Large Ribosomal Subunit

(2014) A. Amunts et al.   SCIENCE

Structure of the large ribosomal subunit from human mitochondria

(2014) A. Brown et al.   SCIENCE

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine

(2014) Wilson Wong et al.   eLife

Cryo-EM enters a new era

(2014) Werner Kühlbrandt   eLife

Structure of the TRPV1 ion channel determined by electron cryo-microscopy

(2013) Maofu Liao et al.   NATURE

Architecture of the large subunit of the mammalian mitochondrial ribosome

(2013) Basil J. Greber et al.   NATURE

Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

(2013) Xueming Li et al.   NATURE METHODS

Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles

(2013) Xiao-chen Bai et al.   eLife

Molecular Model of the Human 26S Proteasome

(2012) Paula C.A. da Fonseca et al.   MOLECULAR CELL

Prevention of overfitting in cryo-EM structure determination

(2012) Sjors H W Scheres et al.   NATURE METHODS

Near-atomic resolution structural model of the yeast 26S proteasome

(2012) F. Beck et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reaching the Information Limit in Cryo-EM of Biological Macromolecules: Experimental Aspects

(2011) Robert M. Glaeser et al.   BIOPHYSICAL JOURNAL

A Bayesian View on Cryo-EM Structure Determination

(2011) Sjors H.W. Scheres   JOURNAL OF MOLECULAR BIOLOGY

Monolithic microfluidic mixing–spraying devices for time-resolved cryo-electron microscopy

(2009) Zonghuan Lu et al.   JOURNAL OF STRUCTURAL BIOLOGY