4.7 Article

Biochemical characteristics of the mutant xylanase T-XynC(122)C(166) and production of xylooligosaccharides from corncobs

期刊

INDUSTRIAL CROPS AND PRODUCTS
卷 142, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.indcrop.2019.111848

关键词

Auto-hydrolysis; Corncobs; Hydrolysis characteristics; Xylanase; Xylooligosaccharides

资金

  1. Beijing Natural Science Foundation [6164029]
  2. General Project of Scientific Research Program of Beijing Municipal Education Commission [KM201910011006]
  3. National Key R&D Program of China [2017YFD040020601]

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The thermostable recombinant xylanase, T-XynC(122)C(166), was investigated for its biochemical characteristics and capacity to produce xylooligosaccharides from corncobs pretreated through auto-hydrolysis. T-XynC(122)C(166) showed optimal activity at pH 6.5 and 75 degrees C, and is an acidophilic and thermostable xylanase with a K-m of 6.08 mg/mL towards beechwood xylan. Hydrolysis of xylan into xylooligosaccharides was through an endohydrolytic mode of action. Although xylotriose and xylobiose were the main hydrolysates produced from different xylans by T-XynC(122)C(166), their compositions and yields were substrate dependent. T-XynC(122)C(166) showed the highest degradation efficiency towards beechwood xylan, followed by oat-spelt xylan and birchwood xylan. T-XynC(122)C(166) rapidly hydrolyzed xylotetraose into xylotriose and xylobiose through transglycosylation, whereas xylotriose was hydrolyzed slowly and xylobiose was not hydrolyzed. The xylooligosaccharide yield from corncobs was determined to be 26.4% when treated with T-XynC(122)C(166) (5 U/mL) at pH 5.5 and 50 degrees C for 2 h, indicating its high potential for xylooligosaccharide production from agricultural waste.

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