Single cell approaches to address adipose tissue stromal cell heterogeneity

Integrative single-cell analysis

(2019) Tim Stuart et al.   NATURE REVIEWS GENETICS

Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH+

(2019) Chee-Huat Linus Eng et al.   NATURE

Adipose Tissue Dysfunction as Determinant of Obesity-Associated Metabolic Complications

(2019) Michele Longo et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Identification of a mesenchymal progenitor cell hierarchy in adipose tissue

(2019) David Merrick et al.   SCIENCE

Single-Cell RNA-Seq Technologies and Related Computational Data Analysis

(2019) Geng Chen et al.   Frontiers in Genetics

Comprehensive Integration of Single-Cell Data

(2019) Tim Stuart et al.   CELL

Lipid-Associated Macrophages Control Metabolic Homeostasis in a Trem2-Dependent Manner

(2019) Diego Adhemar Jaitin et al.   CELL

Current best practices in single‐cell RNA‐seq analysis: a tutorial

(2019) Malte D Luecken et al.   Molecular Systems Biology

Low- and high-thermogenic brown adipocyte subpopulations coexist in murine adipose tissue

(2019) Anying Song et al.   JOURNAL OF CLINICAL INVESTIGATION

Dermal adipose tissue has high plasticity and undergoes reversible dedifferentiation in mice

(2019) Zhuzhen Zhang et al.   JOURNAL OF CLINICAL INVESTIGATION

Single cell analysis reveals immune cell–adipocyte crosstalk regulating the transcription of thermogenic adipocytes

(2019) Prashant Rajbhandari et al.   eLife

Mapping gene regulatory networks from single-cell omics data

(2018) Mark W E J Fiers et al.   Briefings in Functional Genomics

Reversible De-differentiation of Mature White Adipocytes into Preadipocyte-like Precursors during Lactation

(2018) Qiong A. Wang et al.   Cell Metabolism

Deconstructing Adipogenesis Induced by β3-Adrenergic Receptor Activation with Single-Cell Expression Profiling

(2018) Rayanne B. Burl et al.   Cell Metabolism

Heterogeneity of adipose tissue in development and metabolic function

(2018) Theresa Schoettl et al.   JOURNAL OF EXPERIMENTAL BIOLOGY

Identifying cell populations with scRNASeq

(2018) Tallulah S. Andrews et al.   MOLECULAR ASPECTS OF MEDICINE

Integrating single-cell transcriptomic data across different conditions, technologies, and species

(2018) Andrew Butler et al.   NATURE BIOTECHNOLOGY

Distinct macrophage populations direct inflammatory versus physiological changes in adipose tissue

(2018) David A. Hill et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

De novo adipocyte differentiation from Pdgfrβ+ preadipocytes protects against pathologic visceral adipose expansion in obesity

(2018) Mengle Shao et al.   Nature Communications

TGR5 signalling promotes mitochondrial fission and beige remodelling of white adipose tissue

(2018) Laura A. Velazquez-Villegas et al.   Nature Communications

Single Cell RNA Sequencing of Rare Immune Cell Populations

(2018) Akira Nguyen et al.   Frontiers in Immunology

A cellular perspective of adipogenesis transcriptional regulation

(2018) Walid Kuri-Harcuch et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Immune cell subset differentiation and tissue inflammation

(2018) Pu Fang et al.   Journal of Hematology & Oncology

RNA velocity of single cells

(2018) Gioele La Manno et al.   NATURE

A stromal cell population that inhibits adipogenesis in mammalian fat depots

(2018) Petra C. Schwalie et al.   NATURE

Identification of functionally distinct fibro-inflammatory and adipogenic stromal subpopulations in visceral adipose tissue of adult mice

(2018) Chelsea Hepler et al.   eLife

An Introduction to the Analysis of Single-Cell RNA-Sequencing Data

(2018) Aisha A. AlJanahi et al.   Molecular Therapy-Methods & Clinical Development

Advantages of Single-Nucleus over Single-Cell RNA Sequencing of Adult Kidney: Rare Cell Types and Novel Cell States Revealed in Fibrosis

(2018) Haojia Wu et al.   JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY

Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems

(2018) Xiannian Zhang et al.   MOLECULAR CELL

RNA Velocity: Molecular Kinetics from Single-Cell RNA-Seq

(2018) Valentine Svensson et al.   MOLECULAR CELL

Single-nucleus and single-cell transcriptomes compared in matched cortical cell types

(2018) Trygve E. Bakken et al.   PLoS One

The expanding problem of adipose depot remodeling and postnatal adipocyte progenitor recruitment

(2017) Chelsea Hepler et al.   MOLECULAR AND CELLULAR ENDOCRINOLOGY

Comparative Analysis of Single-Cell RNA Sequencing Methods

(2017) Christoph Ziegenhain et al.   MOLECULAR CELL

SCENIC: single-cell regulatory network inference and clustering

(2017) Sara Aibar et al.   NATURE METHODS

Massively parallel single-nucleus RNA-seq with DroNc-seq

(2017) Naomi Habib et al.   NATURE METHODS

Scaling by shrinking: empowering single-cell 'omics' with microfluidic devices

(2017) Sanjay M. Prakadan et al.   NATURE REVIEWS GENETICS

Health Effects of Overweight and Obesity in 195 Countries over 25 Years

(2017)   NEW ENGLAND JOURNAL OF MEDICINE

Massively parallel digital transcriptional profiling of single cells

(2017) Grace X. Y. Zheng et al.   Nature Communications

Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation

(2017) Pablo Bora et al.   Stem Cell Research & Therapy

Distinct cellular and molecular mechanisms for β3 adrenergic receptor-induced beige adipocyte formation

(2017) Yuwei Jiang et al.   eLife

Vital Signs: Trends in Incidence of Cancers Associated with Overweight and Obesity — United States, 2005–2014

(2017) C. Brooke Steele et al.   MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT

Metabolic heterogeneity of activated beige/brite adipocytes in inguinal adipose tissue

(2017) Yun-Hee Lee et al.   Scientific Reports

Adipogenic role of alternatively activated macrophages in β-adrenergic remodeling of white adipose tissue

(2016) Yun-Hee Lee et al.   AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY

Pdgfrβ+ Mural Preadipocytes Contribute to Adipocyte Hyperplasia Induced by High-Fat-Diet Feeding and Prolonged Cold Exposure in Adult Mice

(2016) Lavanya Vishvanath et al.   Cell Metabolism

De novo generation of adipocytes from circulating progenitor cells in mouse and human adipose tissue

(2016) Kathleen M. Gavin et al.   FASEB JOURNAL

Worldwide trends in diabetes since 1980: a pooled analysis of 751 population-based studies with 4·4 million participants

(2016)   LANCET

Single-cell barcoding and sequencing using droplet microfluidics

(2016) Rapolas Zilionis et al.   Nature Protocols

Targeting adipose tissue in the treatment of obesity-associated diabetes

(2016) Christine M. Kusminski et al.   NATURE REVIEWS DRUG DISCOVERY

Enrichr: a comprehensive gene set enrichment analysis web server 2016 update

(2016) Maxim V. Kuleshov et al.   NUCLEIC ACIDS RESEARCH

Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons

(2016) Naomi Habib et al.   SCIENCE

Mouse strains to study cold-inducible beige progenitors and beige adipocyte formation and function

(2016) Daniel C. Berry et al.   Nature Communications

Adipose Tissue Remodeling: Its Role in Energy Metabolism and Metabolic Disorders

(2016) Sung Sik Choe et al.   Frontiers in Endocrinology

Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells

(2015) Allon M. Klein et al.   CELL

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

(2015) Evan Z. Macosko et al.   CELL

The limited storage capacity of gonadal adipose tissue directs the development of metabolic disorders in male C57Bl/6J mice

(2015) Lianne van Beek et al.   DIABETOLOGIA

The increasingly complex regulation of adipocyte differentiation

(2015) Sylvia P Poulos et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Cellular origins of cold-induced brown adipocytes in adult mice

(2015) Yun-Hee Lee et al.   FASEB JOURNAL

Defining cell types and states with single-cell genomics

(2015) Cole Trapnell   GENOME RESEARCH

Brown and beige fat in humans: thermogenic adipocytes that control energy and glucose homeostasis

(2015) Labros Sidossis et al.   JOURNAL OF CLINICAL INVESTIGATION

Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity

(2015) Elise Jeffery et al.   NATURE CELL BIOLOGY

Computational and analytical challenges in single-cell transcriptomics

(2015) Oliver Stegle et al.   NATURE REVIEWS GENETICS

Visceral Adipose Tissue Mesothelial Cells: Living on the Edge or Just Taking Up Space?

(2015) Olga T. Gupta et al.   TRENDS IN ENDOCRINOLOGY AND METABOLISM

Associations of Different Adipose Tissue Depots with Insulin Resistance: A Systematic Review and Meta-analysis of Observational Studies

(2015) Mingzhi Zhang et al.   Scientific Reports

Pharmacological Activation of Thyroid Hormone Receptors Elicits a Functional Conversion of White to Brown Fat

(2015) Jean Z. Lin et al.   Cell Reports

Highly Selective In Vivo Labeling of Subcutaneous White Adipocyte Precursors with Prx1-Cre

(2015) Joan Sanchez-Gurmaches et al.   Stem Cell Reports

Wt1, the mesothelium and the origins and heterogeneity of visceral fat progenitors

(2015) You-Ying Chau et al.   Adipocyte

Exploring the activated adipogenic niche: Interactions of macrophages and adipocyte progenitors

(2014) Yun-Hee Lee et al.   CELL CYCLE

Browning of White Adipose Cells by Intermediate Metabolites: An Adaptive Mechanism to Alleviate Redox Pressure

(2014) A. Carriere et al.   DIABETES

Visceral and subcutaneous fat have different origins and evidence supports a mesothelial source

(2014) You-Ying Chau et al.   NATURE CELL BIOLOGY

Adipocytes arise from multiple lineages that are heterogeneously and dynamically distributed

(2014) Joan Sanchez-Gurmaches et al.   Nature Communications

Pref-1 Marks Very Early Mesenchymal Precursors Required for Adipose Tissue Development and Expansion

(2014) Carolyn S. Hudak et al.   Cell Reports

Characterization of Cre Recombinase Activity for In Vivo Targeting of Adipocyte Precursor Cells

(2014) Katherine C. Krueger et al.   Stem Cell Reports

Low abdominal subcutaneous preadipocyte adipogenesis is associated with visceral obesity, visceral adipocyte hypertrophy, and a dysmetabolic state

(2014) Julie Lessard et al.   Adipocyte

Adipose tissue plasticity from WAT to BAT and in between

(2013) Yun-Hee Lee et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

Mechanisms and Metabolic Implications of Regional Differences among Fat Depots

(2013) Tamara Tchkonia et al.   Cell Metabolism

Identification of an Adipogenic Niche for Adipose Tissue Remodeling and Restoration

(2013) Yun-Hee Lee et al.   Cell Metabolism

Bi-directional interconversion of brite and white adipocytes

(2013) Matthias Rosenwald et al.   NATURE CELL BIOLOGY

Characterization of the adipocyte cellular lineage in vivo

(2013) Ryan Berry et al.   NATURE CELL BIOLOGY

Tracking adipogenesis during white adipose tissue development, expansion and regeneration

(2013) Qiong A Wang et al.   NATURE MEDICINE

Mechanisms for Insulin Resistance: Common Threads and Missing Links

(2012) Varman T. Samuel et al.   CELL

Zfp423 Expression Identifies Committed Preadipocytes and Localizes to Adipose Endothelial and Perivascular Cells

(2012) Rana K. Gupta et al.   Cell Metabolism

In Vivo Identification of Bipotential Adipocyte Progenitors Recruited by β3-Adrenoceptor Activation and High-Fat Feeding

(2012) Yun-Hee Lee et al.   Cell Metabolism

The Vascular Endothelium of the Adipose Tissue Gives Rise to Both White and Brown Fat Cells

(2012) Khanh-Van Tran et al.   Cell Metabolism

FGF21 regulates PGC-1  and browning of white adipose tissues in adaptive thermogenesis

(2012) f. M. Fisher et al.   GENES & DEVELOPMENT

Adipose tissue heterogeneity: Implication of depot differences in adipose tissue for obesity complications

(2012) Mi-Jeong Lee et al.   MOLECULAR ASPECTS OF MEDICINE

A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis

(2012) Pontus Boström et al.   NATURE

JNK Expression by Macrophages Promotes Obesity-Induced Insulin Resistance and Inflammation

(2012) M. S. Han et al.   SCIENCE

Adipocyte Lineage Cells Contribute to the Skin Stem Cell Niche to Drive Hair Cycling

(2011) Eric Festa et al.   CELL

Preferential Fat Deposition in SubcutaneousVersusVisceral Depots Is Associated with Insulin Sensitivity

(2011) Tracey McLaughlin et al.   JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM

Adipose tissue remodeling and obesity

(2011) Kai Sun et al.   JOURNAL OF CLINICAL INVESTIGATION

Immunity as a link between obesity and insulin resistance

(2011) Nishan S. Kalupahana et al.   MOLECULAR ASPECTS OF MEDICINE

Counting absolute numbers of molecules using unique molecular identifiers

(2011) Teemu Kivioja et al.   NATURE METHODS

Human peritoneal mesothelial cells respond to bacterial ligands through a specific subset of Toll-like receptors

(2011) Chantal S. Colmont et al.   NEPHROLOGY DIALYSIS TRANSPLANTATION

Rapid Cellular Turnover in Adipose Tissue

(2011) Alessandra Rigamonti et al.   PLoS One

The emergence of cold-induced brown adipocytes in mouse white fat depots is determined predominantly by white to brown adipocyte transdifferentiation

(2010) G. Barbatelli et al.   AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM

Body Fat Distribution and Inflammation Among Obese Older Adults With and Without Metabolic Syndrome

(2010) Annemarie Koster et al.   Obesity

Characterization of Structure and Cellular Components of Aspirated and Excised Adipose Tissue

(2010) Hitomi Eto et al.   PLASTIC AND RECONSTRUCTIVE SURGERY

De novo generation of white adipocytes from the myeloid lineage via mesenchymal intermediates is age, adipose depot, and gender specific

(2010) S. M. Majka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fighting Fat with Fat: The Expanding Field of Adipose Stem Cells

(2009) Daniel Zeve et al.   Cell Stem Cell

Adipocyte Turnover: Relevance to Human Adipose Tissue Morphology

(2009) E. Arner et al.   DIABETES

Regulatory Mechanisms for Adipose Tissue M1 and M2 Macrophages in Diet-Induced Obese Mice

(2009) S. Fujisaka et al.   DIABETES

Identification of White Adipocyte Progenitor Cells In Vivo

(2008) Matthew S. Rodeheffer et al.   CELL

Beneficial Effects of Subcutaneous Fat Transplantation on Metabolism

(2008) Thien T. Tran et al.   Cell Metabolism

Dead adipocytes, detected as crown-like structures, are prevalent in visceral fat depots of genetically obese mice

(2008) I. Murano et al.   JOURNAL OF LIPID RESEARCH

Metabolic Dysregulation and Adipose Tissue Fibrosis: Role of Collagen VI

(2008) T. Khan et al.   MOLECULAR AND CELLULAR BIOLOGY

Dynamics of fat cell turnover in humans

(2008) Kirsty L. Spalding et al.   NATURE

White Fat Progenitor Cells Reside in the Adipose Vasculature

(2008) W. Tang et al.   SCIENCE