Single-cell barcoding and sequencing using droplet microfluidics

A Method for Detecting Circulating Tumor Cells Based on the Measurement of Single-Cell Metabolism in Droplet-Based Microfluidics

(2016) Fabio Del Ben et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

T cell fate and clonality inference from single-cell transcriptomes

(2016) Michael J T Stubbington et al.   NATURE METHODS

Discovery in Droplets

(2015) Alexander K. Price et al.   ANALYTICAL CHEMISTRY

Noise Facilitates Transcriptional Control under Dynamic Inputs

(2015) Ryan A. Kellogg et al.   CELL

Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells

(2015) Allon M. Klein et al.   CELL

Data-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with Prognosis

(2015) Jacob H. Levine et al.   CELL

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

(2015) Evan Z. Macosko et al.   CELL

Accurate microfluidic sorting of droplets at 30 kHz

(2015) Adam Sciambi et al.   LAB ON A CHIP

The Technology and Biology of Single-Cell RNA Sequencing

(2015) Aleksandra A. Kolodziejczyk et al.   MOLECULAR CELL

Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells

(2015) Florian Buettner et al.   NATURE BIOTECHNOLOGY

High-Throughput Single-Cell Labeling (Hi-SCL) for RNA-Seq Using Drop-Based Microfluidics

(2015) Assaf Rotem et al.   PLoS One

RNA-Seq of single prostate CTCs implicates noncanonical Wnt signaling in antiandrogen resistance

(2015) D. T. Miyamoto et al.   SCIENCE

Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq

(2015) A. Zeisel et al.   SCIENCE

Combinatorial labeling of single cells for gene expression cytometry

(2015) H. C. Fan et al.   SCIENCE

Scalable microfluidics for single-cell RNA printing and sequencing

(2015) Sayantan Bose et al.   GENOME BIOLOGY

Single-Cell Trajectory Detection Uncovers Progression and Regulatory Coordination in Human B Cell Development

(2014) Sean C. Bendall et al.   CELL

Microfluidic single-cell analysis for systems immunology

(2014) Michael Junkin et al.   LAB ON A CHIP

Deconstructing transcriptional heterogeneity in pluripotent stem cells

(2014) Roshan M. Kumar et al.   NATURE

Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq

(2014) Barbara Treutlein et al.   NATURE

The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells

(2014) Cole Trapnell et al.   NATURE BIOTECHNOLOGY

In-depth determination and analysis of the human paired heavy- and light-chain antibody repertoire

(2014) Brandon J DeKosky et al.   NATURE MEDICINE

Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing

(2014) Dmitry Usoskin et al.   NATURE NEUROSCIENCE

Identification and genetic analysis of cancer cells with PCR-activated cell sorting

(2014) Dennis J. Eastburn et al.   NUCLEIC ACIDS RESEARCH

Microfluidic single-cell whole-transcriptome sequencing

(2014) A. M. Streets et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma

(2014) A. P. Patel et al.   SCIENCE

Single-Cell RNA-Seq Reveals Dynamic, Random Monoallelic Gene Expression in Mammalian Cells

(2014) Q. Deng et al.   SCIENCE

Massively Parallel Single-Cell RNA-Seq for Marker-Free Decomposition of Tissues into Cell Types

(2014) D. A. Jaitin et al.   SCIENCE

Ultrahigh-Throughput Mammalian Single-Cell Reverse-Transcriptase Polymerase Chain Reaction in Microfluidic Drops

(2013) Dennis J. Eastburn et al.   ANALYTICAL CHEMISTRY

Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

(2013) Venkatachalam Chokkalingam et al.   LAB ON A CHIP

High-throughput screening for industrial enzyme production hosts by droplet microfluidics

(2013) Staffan L. Sjostrom et al.   LAB ON A CHIP

Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells

(2013) Alex K. Shalek et al.   NATURE

Tumour heterogeneity in the clinic

(2013) Philippe L. Bedard et al.   NATURE

Quantitative single-cell RNA-seq with unique molecular identifiers

(2013) Saiful Islam et al.   NATURE METHODS

Quantitative assessment of single-cell RNA-sequencing methods

(2013) Angela R Wu et al.   NATURE METHODS

Entering the era of single-cell transcriptomics in biology and medicine

(2013) Rickard Sandberg   NATURE METHODS

Single-cell analysis and sorting using droplet-based microfluidics

(2013) Linas Mazutis et al.   Nature Protocols

Tagmentation-based whole-genome bisulfite sequencing

(2013) Qi Wang et al.   Nature Protocols

Single-cell sequencing-based technologies will revolutionize whole-organism science

(2013) Ehud Shapiro et al.   NATURE REVIEWS GENETICS

Droplet Microfluidics-A Tool for Single-Cell Analysis

(2012) Haakan N. Joensson et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution

(2012) Balint Kintses et al.   CHEMISTRY & BIOLOGY

High-volume production of single and compound emulsions in a microfluidic parallelization arrangement coupled with coaxial annular world-to-chip interfaces

(2012) Takasi Nisisako et al.   LAB ON A CHIP

Droplet microfluidics for amplification-free genetic detection of single cells

(2012) Tushar D. Rane et al.   LAB ON A CHIP

Selective droplet coalescence using microfluidic systems

(2012) Linas Mazutis et al.   LAB ON A CHIP

Droplet microfluidics for high-throughput biological assays

(2012) Mira T. Guo et al.   LAB ON A CHIP

Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells

(2012) Daniel Ramsköld et al.   NATURE BIOTECHNOLOGY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Functional single-cell hybridoma screening using droplet-based microfluidics

(2012) B. E. Debs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification

(2012) Tamar Hashimshony et al.   Cell Reports

Intracellular Protein Determination Using Droplet-Based Immunoassays

(2011) Chiara Martino et al.   ANALYTICAL CHEMISTRY

1-Million droplet array with wide-field fluorescence imaging for digital PCR

(2011) Andrew C. Hatch et al.   LAB ON A CHIP

High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays

(2011) Véronique Lecault et al.   NATURE METHODS

High-throughput microfluidic single-cell RT-qPCR

(2011) A. K. White et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-Performance Single Cell Genetic Analysis Using Microfluidic Emulsion Generator Arrays

(2010) Yong Zeng et al.   ANALYTICAL CHEMISTRY

Single-Cell Multiplex Gene Detection and Sequencing with Microfluidically Generated Agarose Emulsions

(2010) Richard Novak et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology

(2010) Ashleigh B. Theberge et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

High-Throughput Screening of Enzymes by Retroviral Display Using Droplet-Based Microfluidics

(2010) Lucia Granieri et al.   CHEMISTRY & BIOLOGY

Single-cell NF-κB dynamics reveal digital activation and analogue information processing

(2010) Savaş Tay et al.   NATURE

Whole-genome molecular haplotyping of single cells

(2010) H Christina Fan et al.   NATURE BIOTECHNOLOGY

High-throughput injection with microfluidics using picoinjectors

(2010) A. R. Abate et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Preparation of monodisperse emulsions by hydrodynamic size fractionation

(2009) Linas Mazutis et al.   APPLIED PHYSICS LETTERS

Surface acoustic wave (SAW) directed droplet flow in microfluidics for PDMS devices

(2009) Thomas Franke et al.   LAB ON A CHIP

Isolation, incubation, and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement

(2009) Weishan Liu et al.   LAB ON A CHIP

Beating Poisson encapsulation statistics using close-packed ordering

(2009) Adam R. Abate et al.   LAB ON A CHIP

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP

Droplet microfluidic technology for single-cell high-throughput screening

(2009) E. Brouzes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-Throughput Sequencing of the Zebrafish Antibody Repertoire

(2009) J. A. Weinstein et al.   SCIENCE

Development of Quantitative Cell-Based Enzyme Assays in Microdroplets

(2008) Ansgar Huebner et al.   ANALYTICAL CHEMISTRY

Droplet-Based Microfluidic Platforms for the Encapsulation and Screening of Mammalian Cells and Multicellular Organisms

(2008) Jenifer Clausell-Tormos et al.   CHEMISTRY & BIOLOGY

Drop-based microfluidic devices for encapsulation of single cells

(2008) Sarah Köster et al.   LAB ON A CHIP