期刊
JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY
卷 28, 期 8, 页码 848-860出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/10498850.2019.1652715
关键词
UHPLC; histamine poisoning; aquatic product; amino acids
资金
- Seafood Industry Research Fund [00107935]
- Yeoman Fellowship Fund
- Institute of Food and Agricultural Sciences (IFAS) at the University of Florida
Tuna and mahi-mahi are two major fish species responsible for histamine poisoning. This research developed a rapid ultra-high performance liquid chromatography (UHPLC) method to determine amino acids, histamine, and other biogenic amines that can act as co-indicators of histamine poisoning in tuna and mahi-mahi. The modified UHPLC method could simultaneously determine four biogenic amines and 10 major free amino acids in mahi-mahi (Coryphaena hippurus) and yellowfin tuna (Thunnus albacares) within 17.5 min. This UHPLC method showed good linear response, sensitivity, resolution, recovery, repeatability, and number of theoretical plates. The concentrations of detected amino acids, biogenic amines in mahi-mahi and tuna, and their relationships with fish spoilage grade were determined by this UHPLC method. The developed UHPLC method is a rapid and accurate way to monitor quality changes of mahi-mahi and tuna by inspecting the changes of amino acids and biogenic amines.
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