Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli

The y-ome defines the 35% of Escherichia coli genes that lack experimental evidence of function

(2019) Sankha Ghatak et al.   NUCLEIC ACIDS RESEARCH

Very rapid flow cytometric assessment of antimicrobial susceptibility during the apparent lag phase of microbial (re)growth

(2019) Srijan Jindal et al.   MICROBIOLOGY-SGM

Energetic evolution of cellular Transportomes

(2018) Behrooz Darbani et al.   BMC GENOMICS

Insight on specificity of uracil permeases of the NAT/NCS2 family from analysis of the transporter encoded in the pyrimidine utilization operon of Escherichia coli

(2018) Maria Botou et al.   MOLECULAR MICROBIOLOGY

Cellular Uptake of the Atypical Antipsychotic Clozapine Is a Carrier-Mediated Process

(2018) David Dickens et al.   MOLECULAR PHARMACEUTICS

Multidrug efflux pumps: structure, function and regulation

(2018) Dijun Du et al.   NATURE REVIEWS MICROBIOLOGY

A new fluorescent dye accumulation assay for parallel measurements of the ABCG2, ABCB1 and ABCC1 multidrug transporter functions

(2018) Edit Szabó et al.   PLoS One

The membrane transporter PotE is required for virulence in avian pathogenic Escherichia coli (APEC)

(2018) Priscila Regina Guerra et al.   VETERINARY MICROBIOLOGY

Efflux drug transporters at the forefront of antimicrobial resistance

(2017) Tahmina Rahman et al.   EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

The therapeutic potential of targeting ABC transporters to combat multi-drug resistance

(2017) Piyush Bugde et al.   EXPERT OPINION ON THERAPEUTIC TARGETS

Contribution of AcrAB-TolC to multidrug resistance in an Escherichia coli sequence type 131 isolate

(2017) Sabine Schuster et al.   INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

The prototypical proton-coupled oligopeptide transporter YdgR fromEscherichia colifacilitates chloramphenicol uptake into bacterial cells

(2017) Bala K. Prabhala et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cellular Models and In Vitro Assays for the Screening of modulators of P-gp, MRP1 and BCRP

(2017) Mariline Gameiro et al.   MOLECULES

New Roads Leading to Old Destinations: Efflux Pumps as Targets to Reverse Multidrug Resistance in Bacteria

(2017) Gabriella Spengler et al.   MOLECULES

Secondary multidrug efflux pump mutants alter Escherichia coli biofilm growth in the presence of cationic antimicrobial compounds

(2017) Denice C. Bay et al.   RESEARCH IN MICROBIOLOGY

Flow cytometric bacterial cell counts challenge conventional heterotrophic plate counts for routine microbiological drinking water monitoring

(2017) S. Van Nevel et al.   WATER RESEARCH

The E. coli dicarboxylic acid transporters DauA act as a signal transducer by interacting with the DctA uptake system

(2017) Eleni Karinou et al.   Scientific Reports

Enhancing Drug Efficacy and Therapeutic Index through Cheminformatics-Based Selection of Small Molecule Binary Weapons That Improve Transporter-Mediated Targeting: A Cytotoxicity System Based on Gemcitabine

(2017) Justine M. Grixti et al.   Frontiers in Pharmacology

Bifurcation kinetics of drug uptake by Gram-negative bacteria

(2017) David A. Westfall et al.   PLoS One

Novel Piperazine Arylideneimidazolones Inhibit the AcrAB-TolC Pump in Escherichia coli and Simultaneously Act as Fluorescent Membrane Probes in a Combined Real-Time Influx and Efflux Assay

(2016) Jürgen A. Bohnert et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

ATP synthase from Escherichia coli : Mechanism of rotational catalysis, and inhibition with the ε subunit and phytopolyphenols

(2016) Mayumi Nakanishi-Matsui et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Mammalian drug efflux transporters of the ATP binding cassette (ABC) family in multidrug resistance: A review of the past decade

(2016) Zhaolin Chen et al.   CANCER LETTERS

Nile Red and Nile Blue: Applications and Syntheses of Structural Analogues

(2016) Vincent Martinez et al.   CHEMISTRY-A EUROPEAN JOURNAL

Application of fluorescent dye substrates for functional characterization of ABC multidrug transporters at a single cell level

(2016) Zsuzsanna Nerada et al.   CYTOMETRY PART A

Multidrug efflux pumps and their role in antibiotic and antiseptic resistance: a pharmacodynamic perspective

(2016) Sandrine Alibert et al.   Expert Opinion on Drug Metabolism & Toxicology

Efflux as a mechanism of antimicrobial drug resistance in clinical relevant microorganisms: the role of efflux inhibitors

(2016) Clarissa Willers et al.   EXPERT OPINION ON THERAPEUTIC TARGETS

Ask the experts: how to curb antibiotic resistance and plug the antibiotics gap?

(2016) Laura Piddock et al.   Future Medicinal Chemistry

Targeting efflux pumps to overcome antifungal drug resistance

(2016) Ann R Holmes et al.   Future Medicinal Chemistry

In vitro characterization and inhibition of the interaction between ciprofloxacin and berberine against multidrug-resistant Klebsiella pneumoniae

(2016) Xiao-Yuan Zhou et al.   JOURNAL OF ANTIBIOTICS

On the role of a specific insert in acetate permeases (ActP) for tellurite uptake in bacteria: Functional and structural studies

(2016) Roberto Borghese et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

A pipeline for developing and testing staining protocols for flow cytometry, demonstrated with SYBR Green I and propidium iodide viability staining

(2016) Alina Nescerecka et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Transcriptional feedback regulation of efflux protein expression for increased tolerance to and production of n -butanol

(2016) Sergey Boyarskiy et al.   METABOLIC ENGINEERING

Screen for genes involved in radiation survival of Escherichia coli and construction of a reference database

(2016) Neil J. Sargentini et al.   MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS

TransportDB 2.0: a database for exploring membrane transporters in sequenced genomes from all domains of life

(2016) Liam D. H. Elbourne et al.   NUCLEIC ACIDS RESEARCH

Molecular basis for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives

(2016) Hanno Sjuts et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps

(2016) Jessica M. A. Blair et al.   mBio

MetMaxStruct: A Tversky-Similarity-Based Strategy for Analysing the (Sub)Structural Similarities of Drugs and Endogenous Metabolites

(2016) Steve O'Hagan et al.   Frontiers in Pharmacology

Reviving Antibiotics: Efflux Pump Inhibitors That Interact with AcrA, a Membrane Fusion Protein of the AcrAB-TolC Multidrug Efflux Pump

(2016) Narges Abdali et al.   ACS Infectious Diseases

Substrate-dependent dynamics of the multidrug efflux transporter AcrB of Escherichia coli

(2016) Kentaro Yamamoto et al.   Scientific Reports

New Fluorescein Precursors for Live Bacteria Detection

(2015) Celia Guilini et al.   ANALYTICAL CHEMISTRY

An Accurate In Vitro Model of theE. coliEnvelope

(2015) Luke A. Clifton et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Fluorescent Microplate Assay Quantifies Bacterial Efflux and Demonstrates Two Distinct Compound Binding Sites in AcrB

(2015) Ramkumar Iyer et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

YehZYXW of Escherichia coli Is a Low-Affinity, Non-Osmoregulatory Betaine-Specific ABC Transporter

(2015) Shenhui Lang et al.   BIOCHEMISTRY

A Call for Systematic Research on Solute Carriers

(2015) Adrián César-Razquin et al.   CELL

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

(2015) Xian-Zhi Li et al.   CLINICAL MICROBIOLOGY REVIEWS

Nature and uses of fluorescent dyes for drug transporter studies

(2015) Olivier Fardel et al.   Expert Opinion on Drug Metabolism & Toxicology

Membrane transporter engineering in industrial biotechnology and whole cell biocatalysis

(2015) Douglas B. Kell et al.   TRENDS IN BIOTECHNOLOGY

What would be the observable consequences if phospholipid bilayer diffusion of drugs into cells is negligible?

(2015) Douglas B. Kell   TRENDS IN PHARMACOLOGICAL SCIENCES

RND-type drug efflux pumps from Gram-negative bacteria: molecular mechanism and inhibition

(2015) Henrietta Venter et al.   Frontiers in Microbiology

Structural basis of RND-type multidrug exporters

(2015) Akihito Yamaguchi et al.   Frontiers in Microbiology

The apparent permeabilities of Caco-2 cells to marketed drugs: magnitude, and independence from both biophysical properties and endogenite similarities

(2015) Steve O’Hagan et al.   PeerJ

A Mass Spectrometry-Based Assay for Improved Quantitative Measurements of Efflux Pump Inhibition

(2015) Adam R. Brown et al.   PLoS One

Inhibitors of ABC Transporters and Biophysical Methods to Study their Activity

(2015) Dominika Bartosiewicz et al.   ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES

Multiple metabolomics of uropathogenic E. coli reveal different information content in terms of metabolic potential compared to virulence factors

(2014) Haitham AlRabiah et al.   ANALYST

Bacterial multidrug efflux pumps: Mechanisms, physiology and pharmacological exploitations

(2014) Jingjing Sun et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Metabolic flux analysis of Escherichia coli knockouts: lessons from the Keio collection and future outlook

(2014) Christopher P Long et al.   CURRENT OPINION IN BIOTECHNOLOGY

Evaluation ofEscherichia coliviability by flow cytometry; a method for determining bacterial responses to antibiotic exposure

(2014) Paola Boi et al.   CYTOMETRY PART B-CLINICAL CYTOMETRY

Implementation of Fourier transform infrared spectroscopy for the rapid typing of uropathogenic Escherichia coli

(2014) S. E. Dawson et al.   EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES

Multidrug efflux pumps in Gram-negative bacteria and their role in antibiotic resistance

(2014) Jessica MA Blair et al.   Future Microbiology

Trends and Exceptions of Physical Properties on Antibacterial Activity for Gram-Positive and Gram-Negative Pathogens

(2014) Dean G. Brown et al.   JOURNAL OF MEDICINAL CHEMISTRY

A ‘rule of 0.5’ for the metabolite-likeness of approved pharmaceutical drugs

(2014) Steve O′Hagan et al.   Metabolomics

The solute carrier SLC35F2 enables YM155-mediated DNA damage toxicity

(2014) Georg E Winter et al.   Nature Chemical Biology

An Optimized SYBR Green I/PI Assay for Rapid Viability Assessment and Antibiotic Susceptibility Testing for Borrelia burgdorferi

(2014) Jie Feng et al.   PLoS One

Translation Enhancing ACA Motifs and Their Silencing by a Bacterial Small Regulatory RNA

(2014) Qi Yang et al.   PLoS Genetics

How drugs get into cells: tested and testable predictions to help discriminate between transporter-mediated uptake and lipoidal bilayer diffusion

(2014) Douglas B. Kell et al.   Frontiers in Pharmacology

Efflux pumps of Gram-negative bacteria: what they do, how they do it, with what and how to deal with them

(2014) Leonard Amaral et al.   Frontiers in Pharmacology

Fluorescent substrates for flow cytometric evaluation of efflux inhibition in ABCB1, ABCC1, and ABCG2 transporters

(2013) J. Jacob Strouse et al.   ANALYTICAL BIOCHEMISTRY

Sensitivity to vinyl phenol derivatives produced by phenolic acid decarboxylase activity in Escherichia coli and several food-borne Gram-negative species

(2013) Hélène Licandro-Seraut et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Inhibition of multidrug efflux as a strategy to prevent biofilm formation

(2013) S. Baugh et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Genetic inactivation of acrAB or inhibition of efflux induces expression of ramA

(2013) A. J. Lawler et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Functional Identification of the Hypoxanthine/Guanine Transporters YjcD and YgfQ and the Adenine Transporters PurP and YicO ofEscherichia coliK-12

(2013) Konstantinos Papakostas et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural basis for the inhibition of bacterial multidrug exporters

(2013) Ryosuke Nakashima et al.   NATURE

Monitoring of Dynamic Microbiological Processes Using Real-Time Flow Cytometry

(2013) Markus Arnoldini et al.   PLoS One

Monitoring microbiological changes in drinking water systems using a fast and reproducible flow cytometric method

(2013) E.I. Prest et al.   WATER RESEARCH

Regulating DNA Replication in Bacteria

(2013) K. Skarstad et al.   Cold Spring Harbor Perspectives in Biology

Identification of Escherichia coli biomarkers responsive to various lignin-hydrolysate compounds

(2012) Siseon Lee et al.   BIORESOURCE TECHNOLOGY

Genome-wide assessment of the carriers involved in the cellular uptake of drugs: a model system in yeast

(2012) Karin Lanthaler et al.   BMC BIOLOGY

Characterization of three novel mechanosensitive channel activities inEscherichia coli

(2012) Michelle D. Edwards et al.   Channels

Microbial Efflux Pump Inhibition: Tactics and Strategies

(2012) George P. Tegos et al.   CURRENT PHARMACEUTICAL DESIGN

The promiscuous binding of pharmaceutical drugs and their transporter-mediated uptake into cells: what we (need to) know and how we can do so

(2012) Douglas B. Kell et al.   DRUG DISCOVERY TODAY

Identification and Analysis of the Putative Pentose Sugar Efflux Transporters in Escherichia coli

(2012) Khushnuma Koita et al.   PLoS One

Structure and function of polyamine-amino acid antiporters CadB and PotE in Escherichia coli

(2011) Hideyuki Tomitori et al.   AMINO ACIDS

Pharmaceutical drug transport: the issues and the implications that it is essentially carrier-mediated only

(2011) Douglas B. Kell et al.   DRUG DISCOVERY TODAY

Metabolic regulation of Escherichia coli and its phoB and phoR genes knockout mutants under phosphate and nitrogen limitations as well as at acidic condition

(2011) Lolo Marzan et al.   Microbial Cell Factories

Determination of Real-Time Efflux Phenotypes in Escherichia coli AcrB Binding Pocket Phenylalanine Mutants Using a 1,2′-Dinaphthylamine Efflux Assay

(2011) Jürgen A. Bohnert et al.   PLoS One

Optimized Nile Red Efflux Assay of AcrAB-TolC Multidrug Efflux System Shows Competition between Substrates

(2010) J. A. Bohnert et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Expression of Multidrug Efflux Pump GenesacrAB-tolC,mdfA, andnorEinEscherichia coliClinical Isolates as a Function of Fluoroquinolone and Multidrug Resistance

(2010) Michelle C. Swick et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Viability states of bacteria-Specific mechanisms of selected probes

(2010) Heike Sträuber et al.   CYTOMETRY PART A

Lipophilicity in drug discovery

(2010) Michael J Waring   Expert Opinion on Drug Discovery

TolC dependency of multidrug efflux systems in Salmonella enterica serovar Typhimurium

(2010) Tsukasa Horiyama et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

A 96-well plate fluorescence assay for assessment of cellular permeability and active efflux in Salmonella enterica serovar Typhimurium and Escherichia coli

(2010) N. G. Coldham et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Estimation of the electric plasma membrane potential difference in yeast with fluorescent dyes: comparative study of methods

(2010) Antonio Peña et al.   JOURNAL OF BIOENERGETICS AND BIOMEMBRANES

Identification and Functions of Amino Acid Residues in PotB and PotC Involved in Spermidine Uptake Activity

(2010) Kyohei Higashi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Past, present and future applications of flow cytometry in aquatic microbiology

(2010) Yingying Wang et al.   TRENDS IN BIOTECHNOLOGY

The Implication of YggT ofEscherichia coliin Osmotic Regulation

(2009) Tomokazu ITO et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Efflux-Mediated Antifungal Drug Resistance

(2009) R. D. Cannon et al.   CLINICAL MICROBIOLOGY REVIEWS

Use of resazurin to detect mefloquine as an efflux-pump inhibitor in Pseudomonas aeruginosa and Escherichia coli

(2009) Faustino Vidal-Aroca et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Identification of Novel Serological Biomarkers for Inflammatory Bowel Disease Using Escherichia coli Proteome Chip

(2009) Chien-Sheng Chen et al.   MOLECULAR & CELLULAR PROTEOMICS

The topology of drug–target interaction networks: implicit dependence on drug properties and target families

(2009) Jordi Mestres et al.   Molecular BioSystems

Update on the Keio collection of Escherichia coli single-gene deletion mutants

(2009) Natsuko Yamamoto et al.   Molecular Systems Biology

A coordinated network of transporters with overlapping specificities provides a robust survival strategy

(2009) N. Tal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli

(2009) Laura Paixão et al.   Journal of Biological Engineering

RamA Confers Multidrug Resistance in Salmonella enterica via Increased Expression of acrB, Which Is Inhibited by Chlorpromazine

(2008) A. M. Bailey et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

High-Throughput Flow Cytometry to Detect Selective Inhibitors of ABCB1, ABCC1, and ABCG2 Transporters

(2008) Irena Ivnitski-Steele et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Poly-3-hydroxybutyrate Synthase from the Periplasm of Escherichiacoli

(2008) Dongsheng Dai et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Carrier-mediated cellular uptake of pharmaceutical drugs: an exception or the rule?

(2008) Paul D. Dobson et al.   NATURE REVIEWS DRUG DISCOVERY

Extrinsic Fluorescent Dyes as Tools for Protein Characterization

(2008) Andrea Hawe et al.   PHARMACEUTICAL RESEARCH

Rapid, cultivation-independent assessment of microbial viability in drinking water

(2008) Michael Berney et al.   WATER RESEARCH