期刊
BIORESOURCE TECHNOLOGY
卷 114, 期 -, 页码 450-456出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2012.02.085
关键词
Lignin; Hydrolysate; Transcriptome; Biomarker; Ferulic acid
资金
- National Research Foundation (NRF) of Korea through the Ministry of Education, Science and Technology [NRF-2009-C1AAA001-2009-0093479]
- Hanyang University [HY-201100000000233-N]
Aberrations in the growth and transcriptome of Escherichia coli str. BL21(DE3) were determined when exposed to varying concentrations of ferulic acid (0.25-1 g/L), an aromatic carboxylic acid identified within lignin-cellulose hydrolysate samples. The expression of several individual genes (aaeA, aaeB, inaA and marA) was significantly induced, i.e., more than 4-fold, and thus these genes and the heat shock response gene htpG were selected as biomarkers to monitor E. coli's responses to five additional hydrolysate-related compounds, including vanillic acid, coumaric acid, 4-hydroxybenzoic acid, ferulaldehyde and furfural. While all of the biomarkers showed dose-dependent responses to most of the compounds, expression of aaeA and aaeB showed the greatest induction (5-30-fold) for all compounds tested except furfural. Lastly, the marA, inaA and htpG genes all showed higher expression levels when the culture was exposed to spruce hydrolysate samples, demonstrating the potential use of these genes as biomarkers. (C) 2012 Elsevier Ltd. All rights reserved.
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