Article
Chemistry, Analytical
Darja Lavogina, Helen Lust, Maris-Johanna Tahk, Tonis Laasfeld, Hans Vellama, Naila Nasirova, Markus Vardja, Kattri-Liis Eskla, Andres Salumets, Ago Rinken, Jana Jaal
Summary: Since 1991, the method of converting resazurin to resorufin has been widely used to measure viability in mammalian cell culture and primary cells. However, there have been variations in the assay protocols used by different research groups, and systematic optimization of the assay has been lacking. In this study, we conducted extensive research to optimize the experimental protocols for resazurin-based viability sensing. The optimization guidelines provided in this study are useful for beginners and experienced scientists in the field.
Article
Chemistry, Physical
Aaron D. Erlich, Nicholas P. Dogantzis, Lara Al Nubani, Lavinia A. Trifoi, Gregory K. Hodgson, Stefania Impellizzeri
Summary: Using photochemical reactions to alter the spectroscopic properties of organic chromophores can lead to the design of molecule-based logic circuits and data encryption strategies. By utilizing optical signals to read, write, and encrypt data, this approach shows potential for data protection.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Review
Biochemistry & Molecular Biology
Andre Bittencourt Lorusso, Joao Antonio Carrara, Carolina Deuttner Neumann Barroso, Felipe Francisco Tuon, Helisson Faoro
Summary: Antimicrobial resistance is a global public health crisis caused by the emergence of resistant organisms and different mechanisms of antibiotic resistance. Efflux pumps, particularly in Gram-negative bacteria like Pseudomonas aeruginosa, play a significant role in multidrug resistance and extensively drug-resistant bacteria. Understanding their function, structure, and regulation is essential in combating antibiotic resistance and developing new drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Oksana Yanshyna, Michal J. Bialek, Oleg Chashchikhin, Rafal Klajn
Summary: Confining the resazurin/resorufin redox pair within a flexible palladium-based coordination cage drastically alters their optical and redox properties.
COMMUNICATIONS CHEMISTRY
(2022)
Article
Microbiology
Olga Lomovskaya, Debora Rubio-Aparicio, Kirk Nelson, Dongxu Sun, Ruslan Tsivkovski, Mariana Castanheira, Jill Lindley, Jeffery Loutit, Michael Dudley
Summary: QPX7728 is an ultrabroad-spectrum beta-lactamase inhibitor that enhances the potency of multiple beta-lactams against P. aeruginosa, with varied results depending on beta-lactamase production and other intrinsic resistance mechanisms.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Gang Wang, Jean-Michel Brunel, Jean-Michel Bolla, Francoise Van Bambeke
Summary: The study found that the polyaminoisoprenyl potentiator NV716 significantly reduced the minimum inhibitory concentration of multiple antibiotics against Pseudomonas aeruginosa and improved their intracellular activity. Compared to other two potentiators, NV716 can resensitize P. aeruginosa to antibiotics both extracellularly and intracellularly, indicating a complex mode of action.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemical Research Methods
Evan F. Haney, Michael J. Trimble, Robert E. W. Hancock
Summary: The protocol outlines simple and reproducible methods for assessing the biofilm inhibition and eradication capacities of novel antibiofilm agents against adherent bacterial biofilms grown in 96-well microtiter plates. It is accessible to any laboratory with a plate reader, requires minimal technical expertise or training, and takes 4 to 5 days to complete.
Article
Infectious Diseases
Rongrong Li, Xuhang Shen, Zhengyuan Li, Jilong Shen, Hao Tang, Huaming Xu, Jilu Shen, Yuanhong Xu
Summary: This study found that the combination of AS101 and mefloquine effectively inhibits biofilm formation, disrupts the bacterial cell wall, and enhances the permeability of the cell membrane of carbapenem-resistant Pseudomonas aeruginosa (CRPA) strains. Furthermore, in vivo experiments demonstrated that AS101-mefloquine treatment significantly reduces the bacterial load in the organs of mice infected with CRPA. These findings suggest the potential clinical application of AS101-mefloquine combination therapy in the treatment of CRPA infections.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Silvia Gervasoni, Jitender Mehla, Charles R. Bergen, Inga V. Leus, Enrico Margiotta, Giuliano Malloci, Andrea Bosin, Attilio V. Vargiu, Olga Lomovskaya, Valentin V. Rybenkov, Paolo Ruggerone, Helen I. Zgurskaya
Summary: This study investigated the dynamics of MexB interactions with its ligands using machine learning, computational and experimental approaches. The results showed that MexB ligands can be effectively expelled or hindered in the transporter cycle based on their interactions with specific residues in MexB binding sites. The findings indicate that MexB ligands fit into a substrate-inhibitor spectrum depending on their physico-chemical properties. This molecular-level information is important for the rational design of new inhibitors and antibiotics less susceptible to the efflux mechanism.
Article
Microbiology
Xiaodong Zhang, Yining Zhao, Luozhu Feng, Mengxin Xu, Yiru Ge, Lingbo Wang, Ying Zhang, Jianming Cao, Yao Sun, Qing Wu, Tieli Zhou
Summary: The combination of mefloquine and colistin showed good antibacterial activity against colistin-resistant P. aeruginosa in vitro, inhibiting biofilm formation and eradicating mature biofilms. This synergistic effect was also observed in vivo, increasing the survival rate of G. mellonella larvae and highlighting the potential of this combination as an alternative therapy for colistin-resistant P. aeruginosa infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Ahmed A. Abdelaziz, Amal M. Abo Kamer, Khaled B. Al-Monofy, Lamiaa A. Al-Madboly
Summary: The discussion focuses on the bioprospecting of microorganisms and their bioactive byproducts, specifically pigments. Microbial pigments, such as pyocyanin produced by Pseudomonas aeruginosa, have various benefits including their natural composition, therapeutic effects, and year-round production. This article concentrates on the production, extraction, and biological applications of pyocyanin pigment in biotechnology, engineering, and biology fields.
MICROBIAL CELL FACTORIES
(2023)
Article
Immunology
Wei Feng, Qing Huang, Yu Wang, Qian Yuan, Xiaoyu Li, Peiyuan Xia, Fengjun Sun
Summary: This study aimed to investigate changes in resistance and epidemiological characteristics of Pseudomonas aeruginosa strains isolated from a respiratory department over a ten-year period. The resistance rate of P. aeruginosa strains to antibiotics decreased significantly from 2006-2007 to 2016-2017. The MLST results showed a decrease in genotypes over the ten-year period.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Biology
Wellington S. Miranda, Q. Cong, A. L. Schaefer, E. K. MacLeod, A. Zimenko, D. Baker, E. P. Greenberg
Summary: Researchers have identified selectivity residues within AHL synthases and receptors using covariation methods, demonstrating their importance in the Las system. This study deepens the understanding of how communication systems evolve and diversify.
Article
Microbiology
Maria A. Gomis-Font, Cristina Pitart, Ester del Barrio-Tofino, Yuliya Zboromyrska, Sara Cortes-Lara, Xavier Mulet, Francesc Marco, Jordi Vila, Carla Lopez-Causape, Antonio Oliver
Summary: A Pseudomonas aeruginosa isolate recovered from a patient with ventilator-associated pneumonia after COVID-19 infection showed resistance to multiple antibiotics due to various gene mutations, including modifications in MexD substrate specificity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Alexandre Tetard, Susie Gaillot, Eline Dubois, Soumaya Aarras, Benoit Valot, Gilles Phan, Patrick Plesiat, Catherine Llanes
Summary: Research has found that Pseudomonas aeruginosa is able to adapt to the strong electrophilic molecule cinnamaldehyde (CNA) by upregulating its intrinsic efflux pump and through other pleiotropic changes, leading to increased resistance. Further evaluation is needed to assess whether multidrug-resistant mutants can emerge in patients using cinnamon essential oil as self-medication.
Article
Microbiology
Ruggero Rusmini, Davide Vecchietti, Raffaella Macchi, Faustino Vidal-Aroca, Giovanni Bertoni
Article
Medicine, General & Internal
Irene Nepita, Raffaele Raimondi, Simonluca Piazza, Alberto Diaspro, Faustino Vidal-Aroca, Salvatore Surdo, Mario R. Romano
Summary: Age-related macular degeneration (AMD) can cause severe vision impairments, such as blindness. Intraocular lenses and optics, particularly implantable miniaturized telescopes, have shown potential in improving vision for AMD patients. In this study, the optical performance of the SING IMT (TM) implantable telescope was evaluated for its potential use in late-stage AMD patients. The results showed that the telescope had even optical transmission, suitable curvature, and minimal geometric aberrations, indicating its feasibility as a high-quality optical element for AMD visual impairment treatments.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Ophthalmology
Alfonso Savastano, Silvia Ferrara, Paola Sasso, Maria Cristina Savastano, Emanuele Crincoli, Tomaso Caporossi, Umberto De Vico, Faustino Vidal Aroca, Giuseppe Francione, Leonardo Sammarco, Gloria Gambini, Chiara Fedeli, Emiliana Di Nardo, Stanislao Rizzo
Summary: This study evaluated the three-month follow-up of SING IMT implant in patients with late-stage AMD and found that it could improve patients' vision and quality of life. However, more research with larger sample sizes and longer follow-up periods is needed to confirm these results.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Biochemical Research Methods
F Vidal-Aroca, M Giannattasio, E Brunelli, A Vezzoli, P Plevani, M Muzi-Falconi, G Bertoni
