Article
Chemistry, Analytical
Siwenjie Qian, Yanju Chen, Xiaofu Wang, Tingzhang Wang, Yang Che, Jian Wu, Zhangying Ye, Junfeng Xu
Summary: Given the possibility of SARS-CoV-2 contamination in food, there is an urgent need for a rapid and accurate nucleic acid detection method to ensure food safety. In this study, we propose a sensitive and reliable molecular detection method for SARS-CoV-2. The method includes a control mechanism for amplicon contamination and can detect as low as 20 copies of SARS-CoV-2 in one reaction. The test can be completed in 45 minutes using a heating block and an ultraviolet lamp, making it potentially suitable for field detection.
Article
Chemistry, Analytical
Jiarui Zhang, Rong Zhao, Kejun Dong, Wan Shu, Sitian Wei, Yuwei Yao, Jun Zhang, Shuangge Liu, Wei Zhang, Hongbo Wang
Summary: DNA damage is a major cause of diseases, and UDG plays a crucial role in repairing DNA damage and serves as an early diagnostic marker and prognostic indicator for cancer. To meet the clinical demand, a simple, low-background, ultra-sensitive UDG activity detection method is urgently needed.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Medicine, Research & Experimental
Miaojin Zhou, Chunhua Zhang, Miaomiao Chen, Zhiqing Hu, Menglin Li, Zhuo Li, Lingqian Wu, Desheng Liang
Summary: In this study, a CRISPR-based biosensor was developed for sensitive detection of nucleic acids. A barcode-based Cas12a-mediated DNA detection strategy was introduced to overcome the limitations of current methods and enable universal detection, simultaneous detection of multiple targets, and quantitative detection. This method showed excellent performance in detecting β-thalassemia and spinal muscular atrophy patients.
Article
Cell Biology
Yunpeng Sui, Shuanghong Peng
Summary: Recent studies have found that changes in copy number variations (CNVs) correlated with transcriptional levels can be found during evolution, embryonic development, and oncogenesis. Proposed mechanisms involve retrotransposons and homologous recombination, explaining the dynamic alterations in CNVs in the genome during different biological processes. This suggests that the genetic information contained in the genome is not only three-dimensional, but will become four-dimensional, carrying more information.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Immunology
Than Linh Quyen, Aaydha Chidambara Vinayaka, Mohsen Golabi, Huynh Van Ngoc, Dang Duong Bang, Anders Wolff
Summary: This study developed a new LAMP assay that can eliminate carryover contamination and rapidly detect SARS-CoV-2. The assay can detect the virus as low as 2 copies/μl within 45 minutes and eliminate a significant amount of contaminants. Analysis of clinical samples showed high agreement with rRT-PCR.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Hui Wu, Cheng Qian, Rui Wang, Cui Wu, Zhen Wang, Liu Wang, Mengyao Zhang, Zunzhong Ye, Fang Zhang, Jin-song He, Jian Wu
Article
Nanoscience & Nanotechnology
Fang Zhang, Biwu Liu, Anand Lopez, Shaoyun Wang, Juewen Liu
Review
Biophysics
Yanju Chen, Cheng Qian, Chengzhi Liu, Hong Shen, Zhijian Wang, Jianfeng Ping, Jian Wu, Huan Chen
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Biophysics
Hui Wu, Cheng Qian, Cui Wu, Zhen Wang, Dacheng Wang, Zunzhong Ye, Jianfeng Ping, Jian Wu, Feng Ji
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Chemistry, Analytical
Rui Wang, Rui Chen, Cheng Qian, Yanan Pang, Jian Wu, Fuyou Li
Summary: The proposed method integrates rapid PCR amplification and Cas12a cleavage, simplifying the operation and avoiding contamination. It demonstrates high detection sensitivity and reliability, and has broad application prospects in fields such as food safety and clinical diagnosis, offering a potential solution for rapid on-site testing.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Analytical
Hui Wu, Xiaoyun Chen, Mengyao Zhang, Xiaofu Wang, Yanju Chen, Cheng Qian, Jian Wu, Junfeng Xu
Summary: The CRISPR/Cas system is playing an important role in biosensing applications, combining with multiple detection techniques for various targets detection. However, there are still challenges that need to be addressed in the future, such as off-target effect, target sequence limitation.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Hui Wu, Yanju Chen, Qunqing Yang, Cheng Peng, Xiaofu Wang, Mengyao Zhang, Siwenjie Qian, Junfeng Xu, Jian Wu
Summary: A rapid and low-cost detection method for V. parahaemolyticus using a chip and CRISPR/Cas12a was established, allowing for detection within 50 minutes with high sensitivity. The method showed great feasibility for real samples detection and has potential applications in food safety analysis and clinical diagnostics, especially in resource-limited areas.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Hui Wu, Yanju Chen, Ya Shi, Liu Wang, Mengyao Zhang, Jian Wu, Huan Chen
Summary: The polypropylene (PP) bag-based method for nucleic acid detection offers the potential for at-home testing, with the ability to visually detect Salmonella typhimurium (St) and SARS-CoV-2 within 1 hour; the combination of magnetic particles and isothermal amplification technology allows for convenient processing. Introducing a logic decision method enhances the reliability of at-home nucleic acid testing.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Yanju Chen, Shuyue Wu, Hui Wu, Peng Cheng, Xiaofu Wang, Siwenjie Qian, Mengyao Zhang, Junfeng Xu, Feng Ji, Jian Wu
Summary: qPCR gray zone testing results in uncertainty, while CRISPR technology can enhance detection signal sensitivity. A CRISPR-based checking method is proposed to solve gray zone problems, effectively eliminating uncertainty in sample classification without increasing workload in PCR detection.
Article
Agriculture, Multidisciplinary
Linlin Bai, Liu Wang, Shuqin Huang, Rong Bai, Xucong Lv, Liping Sun, Fang Zhang, Xiahong Xu
Summary: In this study, we introduced CRISPR/Cas12a to specifically recognize the amplicons of NEAA and convert the signals into fluorescent readouts. The vis-NEAA method takes advantage of the high efficiency of NEAA and successfully bypasses nonspecific amplification. Compared to traditional methods, vis-NEAA is efficient, accurate, and fast, allowing for the detection of low concentrations of Salmonella in egg samples within 20 minutes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Rui Wang, Yongfang Li, Yanan Pang, Fang Zhang, Fuyou Li, Shihua Luo, Chunyan Qian
Summary: In this study, an ultrasensitive and visual in-one-tube detection method for COVID-19 was developed. The method can be completed within half an hour and is of great significance for early detection of the virus. By integrating all reactions in one tube and using magnetic beads for nucleic acid extraction, the method eliminates liquid handling steps and increases sensitivity. The fluorescent results can be directly observed by naked eyes using portable thermocycler and blue light.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Huan Zeng, Shuqin Huang, Yunong Chen, Minshi Chen, Kaiyu He, Caili Fu, Qiang Wang, Fang Zhang, Liu Wang, Xiahong Xu
Summary: A sequence-specific visualization method based on loop-mediated isothermal amplification (LAMP) was developed for the detection of Salmonella in milk. The method used restriction endonuclease and nicking endonuclease to produce amplicons into single-stranded triggers, which further promoted the generation of a G-quadruplex by a DNA machine. The G-quadruplex DNAzyme possessed peroxidase-like activity and catalyzed the color development of ABTS as the readouts. The method demonstrated a sensitivity of 800 CFU/mL when observed with the naked eye and could detect Salmonella in milk within 1.5 h without the need for any sophisticated instrument.
Review
Chemistry, Analytical
Youngung Seok, Michael G. Mauk, Ruijie Li, Cheng Qian
Summary: In resource-limited conditions like the COVID-19 pandemic, on-site detection of diseases using Point-of-care testing (POCT) is crucial for overcoming crises and saving lives. This review introduces recent approaches to respiratory virus detection, analysis trends, and prospects, emphasizing the importance of affordable, sensitive, and rapid testing on simple and portable platforms.
ANALYTICA CHIMICA ACTA
(2023)
Article
Food Science & Technology
Wei-Ling Guo, Ying-Jia Cao, Shi-Ze You, Qi Wu, Fang Zhang, Jin-Zhi Han, Xu-Cong Lv, Ping-Fan Rao, Lian-Zhong Ai, Li Ni
Summary: Ganoderma lucidum ethanol extract (GLE) has a protective effect against alcoholic liver injury by reducing abnormal lipid levels, attenuating hepatic lipid accumulation and pathological changes, ameliorating oxidative stress, regulating gut microbiota, and modulating liver metabolites and gene expression related to fatty acid metabolism, ethanol catabolism, and inflammatory response.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Article
Agricultural Engineering
C. Wu, Z. Wang, H. Wu, Z. Lu, C. Qian, S. Jin, R. Wang, Y. Meng, J. Wu, J. Ping, Y. Ying, Z. Ye
Summary: A novel sample-to-answer optical system for on-site detection of Candidatus Liberibacter asiaticus (Las) was developed, integrating DNA extraction, amplification, and detection into a portable case. With high sensitivity and stability, the system has promising prospects for in-field detection of huanglongbing (HLB) caused by Las.
TRANSACTIONS OF THE ASABE
(2021)
