Review
Biophysics
Xinkuan Cheng, Yaru Li, Jun Kou, Dan Liao, Wenlu Zhang, Lijuan Yin, Shuli Man, Long Ma
Summary: CRISPR/Cas tools have shown great potential in biosensing beyond gene editing. However, the application of CRISPR/Cas-based biosensors for non-nucleic acid targets is still in its early stages. This review summarizes the strategies and prospects of CRISPR/Cas toolboxes in recognizing non-nucleic acid targets, providing valuable information for the expansion of these powerful tools into multiple detection fields.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Chemistry, Analytical
Zhiying Zhao, Minghui Lu, Nan Wang, Yaru Li, Liangjuan Zhao, Qiang Zhang, Shuli Man, Shengying Ye, Long Ma
Summary: Developing consensus, easy-to-use, and conceptually new methods for ensuring food safety is of great significance. Nanomaterials-assisted CRISPR/Cas detection has enormous potential and promising applications in the field of food safety.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Huimin Li, Yi Xie, Fumin Chen, Huiwen Bai, Leshan Xiu, Xiaonong Zhou, Xiaokui Guo, Qinqin Hu, Kun Yin
Summary: Rapid and accurate molecular diagnosis is crucial for precision medicine, food safety, and environmental monitoring. CRISPR/Cas-based detection has become an effective tool for molecular diagnosis due to its outstanding advantages. However, existing methods typically require pre-amplification, and the development of target amplification-free CRISPR/Cas-based detection still faces challenges.
CHEMICAL SOCIETY REVIEWS
(2023)
Review
Chemistry, Physical
Weiran Su, Junru Li, Chen Ji, Congshuo Chen, Yuzheng Wang, Huili Dai, Fengqin Li, Peifeng Liu
Summary: CRISPR/Cas systems are powerful tools for disease biomarkers detection, capable of detecting nucleic acid and non-nucleic acid targets. This review summarizes the principles and characteristics of various CRISPR/Cas systems, and introduces the applications of CRISPR/Cas systems in detecting nucleic and non-nucleic acid targets. The prospects and challenges of their applications in biosensing are also discussed.
Review
Cell Biology
Shuying Feng, Zilong Wang, Aifang Li, Xin Xie, Junjie Liu, Shuxuan Li, Yalan Li, Baiyan Wang, Lina Hu, Lianhe Yang, Tao Guo
Summary: CRISPR/Cas systems have revolutionized gene editing and shown great potential in genetic manipulation. However, they also have limitations such as off-target effects and delivery efficiency. This review comprehensively discusses strategies for improving CRISPR/Cas-induced mutations and explores emerging approaches and future applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Engineering, Environmental
Ying Huang, Qian Wen, Ying Xiong, Yanni Chen, Wang Li, Jiali Ren, Haiyan Zhong
Summary: The advancement of biosensing technology has led to increased demands for precise target recognition and highly sensitive detection. Nanomaterials and CRISPR/Cas have emerged as indispensable contributors, and their integration shows immense potential for diverse applications.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Pharmacology & Pharmacy
Aayushi Lohia, Deepak Kumar Sahel, Mohd Salman, Vivek Singh, Indumathi Mariappan, Anupama Mittal, Deepak Chitkara
Summary: CRISPR/Cas, as an efficient and precise tool, has shown potential in genetic editing for retinal dystrophies. The reversal of gene mutations using CRISPR/Cas9 could be beneficial for the treatment of these conditions. However, the delivery of CRISPR components to the posterior part of the eye remains a major concern.
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Bowei Yuan, Congcong Yuan, Lulu Li, Miao Long, Zeliang Chen
Summary: This article reviews the application of the CRISPR/Cas system in pathogen detection, focusing on the Type II (cas9), Type V (cas12a, cas12b, cas14a), and Type VI (cas13a) systems. The CRISPR/Cas system offers strong specificity, high sensitivity, and convenience for detection, making it suitable for practical applications.
Review
Biochemical Research Methods
Mingzhu Yuan, Ronghua Ding, Shuaiyin Chen, Guangcai Duan
Summary: The CRISPR/Cas system provides a rapid and accurate nucleic acid detection method for on-site POCT, with features such as short detection cycles, low cost, and high sensitivity, which are crucial in interrupting outbreaks of infectious diseases.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Analytical
Yanju Chen, Hui Wu, Siwenjie Qian, Xiaoping Yu, Huan Chen, Jian Wu
Summary: A highly sensitive lead detection method combining DNAzyme and CRISPR system is proposed, which can detect low concentrations of lead ions and visualize the results with a portable 3D printing device, suitable for on-site detection.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Xueqi Li, Rong Li, Zheng Yuan, Zaobing Zhu, Wenting Xu, Yijie Wang, Dabing Zhang, Litao Yang
Summary: In this study, a new method called Cc-qPCR was developed for rapid screening of mutants and identification of their genotypes, as well as evaluating gene editing efficiency. Testing on rice samples showed that Cc-qPCR is an accurate and effective approach, which can enhance the efficiency and range of molecular breeding techniques.
ANALYTICAL CHEMISTRY
(2022)
Article
Microbiology
Funmilayo O. Fapohunda, Seng Qiao, Ye Pan, Huiying Wang, Yuan Liu, Quansheng Chen, Peng Lu
Summary: This article introduces the application of CRISPR-based diagnostic methods in the field of nucleic acid detection. By combining with other methods, this technology can detect viruses, bacteria, and other pathogens, and can directly detect diseases from patient samples. This rapid, sensitive, accurate, and advanced technology is of great significance in diagnosis and detection.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Biochemical Research Methods
Xiang Sun, Dan Ohtan Wang, Jinkai Wang
Summary: This review summarizes the latest CRISPR-Cas13 and Cas9 toolkits for site-specific manipulation of m(6)A, including fundamental components, on-target efficiency, editing window, PAM/PFS requirement, subcellularly localized targeting, and potential limitations. It aims to provide an overview to assist researchers in choosing optimal tools for manipulating m(6)A for different purposes and also points out possible optimization strategies.
Article
Biophysics
Lijuan Yin, Shuli Man, Shengying Ye, Guozhen Liu, Long Ma
Summary: This article highlights the recent advances in virus detection using CRISPR-Cas systems, particularly CRISPR-Cas12a/Cas13a. These systems, known for their sensitivity, specificity, high base resolution, and programmability, are revolutionizing virus detection and offering new possibilities in the field.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Pharmacology & Pharmacy
Guillermo Aquino-Jarquin
Summary: Comparative genomics has led to the discovery of small CRISPR bacterial immune system effectors that have the potential to manipulate eukaryotic genomes. Recently, smaller Cas proteins, such as miniature Cas9, Cas12, and Cas13 proteins, have been identified and shown to be efficient tools for genome and base editing in human cells. These small CRISPR-Cas systems offer a compact size, making them ideal for generating CRISPR-based therapeutics that can overcome in vivo delivery constraints. They provide a promising opportunity for editing pathogenic mutations and targeting RNAs in human cells without causing chromosomal insertions or genome alterations. Therefore, these tiny CRISPR-Cas systems represent new and highly programmable, specific, and efficient platforms that expand the CRISPR toolkit for potential therapeutic applications.
DRUG DISCOVERY TODAY
(2023)
Article
Food Science & Technology
Hui Wu, Cheng Qian, Rui Wang, Cui Wu, Zhen Wang, Liu Wang, Mengyao Zhang, Zunzhong Ye, Fang Zhang, Jin-song He, Jian Wu
Article
Materials Science, Multidisciplinary
Yuanchao Lu, Xunjia Li, Jianfeng Ping, Jin-song He, Jian Wu
ADVANCED MATERIALS TECHNOLOGIES
(2020)
Review
Biophysics
Yanju Chen, Cheng Qian, Chengzhi Liu, Hong Shen, Zhijian Wang, Jianfeng Ping, Jian Wu, Huan Chen
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Biophysics
Hui Wu, Cheng Qian, Cui Wu, Zhen Wang, Dacheng Wang, Zunzhong Ye, Jianfeng Ping, Jian Wu, Feng Ji
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Chemistry, Analytical
Yuanchao Lu, Yawen Zheng, Hao Zhang, Xiangxiang He, Qunqing Yang, Jian Wu
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Analytical
Rui Wang, Rui Chen, Cheng Qian, Yanan Pang, Jian Wu, Fuyou Li
Summary: The proposed method integrates rapid PCR amplification and Cas12a cleavage, simplifying the operation and avoiding contamination. It demonstrates high detection sensitivity and reliability, and has broad application prospects in fields such as food safety and clinical diagnosis, offering a potential solution for rapid on-site testing.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Mengyao Zhang, Chengzhi Liu, Ya Shi, Jun Wu, Jian Wu, Huan Chen
Review
Chemistry, Analytical
Yanju Chen, Yang Liu, Ya Shi, Jianfeng Ping, Jian Wu, Huan Chen
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Analytical
Yuanchao Lu, Qunqing Yang, Jian Wu
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2020)
Article
Chemistry, Applied
Hui Wu, Xianjun Zhang, Baoqiang Wu, Cheng Qian, Fang Zhang, Liu Wang, Zunzhong Ye, Jian Wu
Article
Biophysics
Hui Wu, Yanju Chen, Qunqing Yang, Cheng Peng, Xiaofu Wang, Mengyao Zhang, Siwenjie Qian, Junfeng Xu, Jian Wu
Summary: A rapid and low-cost detection method for V. parahaemolyticus using a chip and CRISPR/Cas12a was established, allowing for detection within 50 minutes with high sensitivity. The method showed great feasibility for real samples detection and has potential applications in food safety analysis and clinical diagnostics, especially in resource-limited areas.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Hui Wu, Yanju Chen, Ya Shi, Liu Wang, Mengyao Zhang, Jian Wu, Huan Chen
Summary: The polypropylene (PP) bag-based method for nucleic acid detection offers the potential for at-home testing, with the ability to visually detect Salmonella typhimurium (St) and SARS-CoV-2 within 1 hour; the combination of magnetic particles and isothermal amplification technology allows for convenient processing. Introducing a logic decision method enhances the reliability of at-home nucleic acid testing.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Yanju Chen, Shuyue Wu, Hui Wu, Peng Cheng, Xiaofu Wang, Siwenjie Qian, Mengyao Zhang, Junfeng Xu, Feng Ji, Jian Wu
Summary: qPCR gray zone testing results in uncertainty, while CRISPR technology can enhance detection signal sensitivity. A CRISPR-based checking method is proposed to solve gray zone problems, effectively eliminating uncertainty in sample classification without increasing workload in PCR detection.
Article
Agricultural Engineering
C. Wu, Z. Wang, H. Wu, Z. Lu, C. Qian, S. Jin, R. Wang, Y. Meng, J. Wu, J. Ping, Y. Ying, Z. Ye
Summary: A novel sample-to-answer optical system for on-site detection of Candidatus Liberibacter asiaticus (Las) was developed, integrating DNA extraction, amplification, and detection into a portable case. With high sensitivity and stability, the system has promising prospects for in-field detection of huanglongbing (HLB) caused by Las.
TRANSACTIONS OF THE ASABE
(2021)
Review
Chemistry, Analytical
Claudia Campanale, Silvia Galafassi, Francesca Di Pippo, Iulian Pojar, Carmine Massarelli, Vito Felice Uricchio
Summary: Biodegradable plastic mulches (BDMs) have the potential to be an eco-friendly alternative to conventional plastic mulches in agriculture. However, their long-term impacts on soil ecosystems are still uncertain, and further research is needed. Incorporating BDMs into the soil may stimulate microbial activity, but there is also a risk of incomplete degradation and release of residual microplastics and additives.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Xuechi Yin, Sijie Liu, Deepak Kukkar, Jianlong Wang, Daohong Zhang, Ki-Hyun Kim
Summary: The lateral flow immunoassay strip is a classic and dominant tool in point-of-care detection, known for its portability and simplicity. However, current colored nanomaterials used as signal labels in LFIA have limited sensing potential, leading to the recognition of composite nanoparticles as alternatives to enhance sensitivity, signal readout diversity, anti-matrix interference, and cost-effectiveness.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Yaxin Cao, Longyi Shao, Timothy P. Jones, Wenjing Deng, M. Santosh, Pengju Liu, Cheng-Xue Yang, Yaowei Li, Daizhou Zhang, Kelly Berube
Summary: The toxicity of fibrous particles in ambient air can be higher than other types of particles. Microfibers can be organic or inorganic and their presence in the atmosphere as a result of human activity raises concerns for environmental and public health. Accurate collection, identification, and understanding of the health hazards associated with these fibers are crucial for mitigation strategies and protection of public health.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Chenchen Xie, Chen Meng, Huilin Liu, Baoguo Sun
Summary: The development of food hazard factor (FHF) detection has been moving towards on-site inspection and mobile law enforcement, calling for simple, fast, and affordable analysis technology to ensure food safety and quality. This review comprehensively summarizes the progress in research on smartphone-assisted MIP optosensors, focusing on the key issues of accuracy and sensitivity.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Guillem Peris-Pastor, Cristian Azorin, Jose Grau, Juan L. Benede, Alberto Chisvert
Summary: Green Analytical Chemistry plays an important role in every stage of method development, including sample preparation. Extraction techniques have evolved from conventional ones to a variety of miniaturized techniques in order to enhance analytical performance and ensure safety for operators and the environment. Green metric tools are used to evaluate the impact and sustainability of methods, with AGREEprep selected as the most suitable tool for evaluating published methods in various applications. The miniaturization of sample preparation strategies has led to the use of less and safer solvents, reduced waste generation and energy consumption, and improved portability and throughput.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Helena Hruskova, Roman Reminek, Frantisek Foret
Summary: This review article summarizes the progress and recent studies in offline electromigration preparative techniques between 2018 and 2023. It covers various techniques including electrophoresis, free-flow electrophoresis, isoelectric focusing, and isotachophoresis, providing theoretical aspects, trends, and solutions to existing limitations in each technique.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Mayank Garg, Nicole Pamme
Summary: This review focuses on the removal strategies of templates from molecularly imprinted polymers (MIPs) used in diagnostic biosensors. Chemical-based and electrochemical-based template extraction approaches are summarized and evaluated, providing guidance for researchers in the fields of analytical chemistry, diagnostics, and materials science for the design of MIP-based sensors.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Ivneet Banga, Anirban Paul, Nathan Kodjo Mintah Churcher, Ruchita Mahesh Kumar, Sriram Muthukumar, Shalini Prasad
Summary: Breathomics is an emerging field that focuses on analyzing exhaled breath to diagnose and monitor respiratory diseases. Electrochemical sensors, with their high sensitivity, selectivity, and low cost, play a significant role in breathomics and can be integrated into portable devices for point-of-care diagnosis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Fabjola Bilo, Paola Cirelli, Laura Borgese
Summary: This review explores the application of X-ray fluorescence (XRF) spectrometry for elemental analysis of particulate matter (PM) in air quality monitoring. It discusses the fundamentals of XRF, experimental configurations, PM sampling devices and substrate, sample preparation strategies, qualitative and quantitative analysis, as well as the challenges faced by XRF in becoming a reliable analytical technique for PM analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Hakim Faraji
Summary: The dispersive liquid-liquid microextraction (DLLME) technique is widely popular in analytical chemistry due to its high efficiency, cost-effectiveness, and simplicity. Recent advancements have addressed the limitations of DLLME, incorporating greener solvents, innovative dispersion strategies, and simplified procedures. These changes have made DLLME a more sustainable and efficient technique.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Sherin F. Hammad, Inas A. Abdallah, Alaa Bedair, Reda M. Abdelhameed, Marcello Locatelli, Fotouh R. Mansour
Summary: Carbon nanomaterials (CNMs) have excellent capabilities in terms of adsorption, enhanced oxidation, and photocatalysis, attracting widespread attention in different fields. Metal-organic frameworks (MOFs) have been widely used due to their regular network structure, porous nature, and large specific surface area. However, the instability of MOFs in aqueous solutions limits their applications in chemical sensing. Recent studies have focused on increasing the stability of MOFs in water-based matrices through chemical modifications using CNMs.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Alexandra Pape, Oliver J. Schmitz
Summary: Low temperature plasmas based on dielectric barrier discharges have emerged as a recent development in ion source research. These ion sources are applicable to heat-sensitive analytes and can ionize a wide range of compounds. Despite intensive research on important parameters such as configuration, shape, size, and materials in the past two decades, the actual ionization mechanism for all plasma gases has not been fully elucidated. This review provides an overview of atmospheric pressure ion sources, with a focus on plasma and dielectric barrier discharge based ion sources, summarizing the reactions occurring in the plasma and discussing the applications developed by different research groups.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Hai Xiong, Fengli Li, Linyu Zeng, Qiyu Lei
Summary: This review provides an overview of the emerging trends in the use of functional COF nanomaterials as biosensors for DNA/RNA detection. It summarizes the construction of DNA/RNA hybridized COF nanoprobes and their potential applications in bioanalyte detection, biosensing, biomarker imaging, and clinical biomedicine.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Bing Yan
Summary: This article summarizes the recent progress in logic gate computing and operation based on multiple luminescence response and chemical sensing for lanthanide functionalized MOF hybrid systems. The article first describes multi-color luminescence and the subsequent multiple luminescence response. The basic concept of logic gate operation and the advantages of lanthanide-based hybrid materials are then introduced. Reports on logic gate operation using pure lanthanide MOFs are provided, followed by a detailed summary of logic gate operations using lanthanide functionalized MOF hybrid materials. Additionally, the article discusses the practical luminescent detection of analytes by correlating the luminescence responsive signals and the concentration of different analytes. Finally, the article concludes with a prospect for this topic.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
Review
Chemistry, Analytical
Brij Mohan, Gurjaspreet Singh, Rakesh Kumar Gupta, Pawan Kumar Sharma, Alexander A. Solovev, Armando J. L. Pombeiro, Peng Ren
Summary: Porous materials, particularly hydrogen-bonded organic frameworks (HOFs), have gained significant attention due to their versatile applications and unique properties such as well-defined crystalline structure and easy regeneration processes. This article provides a comprehensive overview of HOFs, including synthesis methods, hydrogen bonding motifs, recent advancements, and potential applications, offering valuable guidance for further exploration in this dynamic field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2024)
