Analysis of the roles of phosphatidylinositol-4,5-bisphosphate and individual subunits in assembly, localization, and function of Saccharomyces cerevisiae target of rapamycin complex 2

Kinesins relocalize the chromosomal passenger complex to the midzone for spindle disassembly

(2018) Itziar Ibarlucea-Benitez et al.   JOURNAL OF CELL BIOLOGY

Unification of Protein Abundance Datasets Yields a Quantitative Saccharomyces cerevisiae Proteome

(2018) Brandon Ho et al.   Cell Systems

Regulation of actin assembly by PI(4,5)P2 and other inositol phospholipids: An update on possible mechanisms

(2018) Paul A. Janmey et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Decrease in plasma membrane tension triggers PtdIns(4,5)P2 phase separation to inactivate TORC2

(2018) Margot Riggi et al.   NATURE CELL BIOLOGY

Phosphorylation by the stress-activated MAPK Slt2 down-regulates the yeast TOR complex 2

(2018) Kristin L. Leskoske et al.   GENES & DEVELOPMENT

Rab5 GTPases are required for optimal TORC2 function

(2018) Melissa N. Locke et al.   JOURNAL OF CELL BIOLOGY

The Stress-Sensing TORC2 Complex Activates Yeast AGC-Family Protein Kinase Ypk1 at Multiple Novel Sites

(2017) Kristin L. Leskoske et al.   GENETICS

Cryo-EM structure of Saccharomyces cerevisiae target of rapamycin complex 2

(2017) Manikandan Karuppasamy et al.   Nature Communications

Substrate specificity of TOR complex 2 is determined by a ubiquitin-fold domain of the Sin1 subunit

(2017) Hisashi Tatebe et al.   eLife

Functional mapping of yeast genomes by saturated transposition

(2017) Agnès H Michel et al.   eLife

The Stress-Sensing TORC2 Complex Activates Yeast AGC-Family Protein Kinase Ypk1 at Multiple Novel Sites

(2017) Kristin L. Leskoske et al.   GENETICS

TORC2 Structure and Function

(2016) Christl Gaubitz et al.   TRENDS IN BIOCHEMICAL SCIENCES

TOR Complexes and the Maintenance of Cellular Homeostasis

(2016) Sandra Eltschinger et al.   TRENDS IN CELL BIOLOGY

Tor forms a dimer through an N-terminal helical solenoid with a complex topology

(2016) Domagoj Baretić et al.   Nature Communications

Characterization of Fluorescent Proteins for Three- and Four-Color Live-Cell Imaging in S. cerevisiae

(2016) Ryo Higuchi-Sanabria et al.   PLoS One

Advances in Phos-tag-based methodologies for separation and detection of the phosphoproteome

(2015) Eiji Kinoshita et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

TORC2 and eisosomes are spatially interdependent, requiring optimal level of phosphatidylinositol 4, 5-bisphosphate for their integrity

(2015) Katelyn Bartlett et al.   JOURNAL OF BIOSCIENCES

Molecular Basis of the Rapamycin Insensitivity of Target Of Rapamycin Complex 2

(2015) Christl Gaubitz et al.   MOLECULAR CELL

The Phyre2 web portal for protein modeling, prediction and analysis

(2015) Lawrence A Kelley et al.   Nature Protocols

PtdIns(3,4,5)P3-Dependent Activation of the mTORC2 Kinase Complex

(2015) P. Liu et al.   Cancer Discovery

Amino Acids Stimulate TORC1 through Lst4-Lst7, a GTPase-Activating Protein Complex for the Rag Family GTPase Gtr2

(2015) Marie-Pierre Péli-Gulli et al.   Cell Reports

Fungal Membrane Organization: The Eisosome Concept

(2014) Lois M. Douglas et al.   Annual Review of Microbiology

ROCC, a conserved region in cohesin's Mcd1 subunit, is essential for the proper regulation of the maintenance of cohesion and establishment of condensation

(2014) Thomas Eng et al.   MOLECULAR BIOLOGY OF THE CELL

Modular peptide binding: From a comparison of natural binders to designed armadillo repeat proteins

(2013) Christian Reichen et al.   JOURNAL OF STRUCTURAL BIOLOGY

mTOR kinase structure, mechanism and regulation

(2013) Haijuan Yang et al.   NATURE

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

(2013) Nathan C Shaner et al.   NATURE METHODS

Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae

(2013) Sidae Lee et al.   PLoS One

An expanded tool kit for the auxin-inducible degron system in budding yeast

(2013) Magdalena Morawska et al.   YEAST

Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways

(2012) Yidi Sun et al.   MOLECULAR BIOLOGY OF THE CELL

Plasma membrane stress induces relocalization of Slm proteins and activation of TORC2 to promote sphingolipid synthesis

(2012) Doris Berchtold et al.   NATURE CELL BIOLOGY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Plasma membrane recruitment and activation of the AGC kinase Ypk1 is mediated by target of rapamycin complex 2 (TORC2) and its effector proteins Slm1 and Slm2

(2012) B. J. Niles et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Target of Rapamycin Complex 2 Signals to Downstream Effector Yeast Protein Kinase 2 (Ypk2) through Adheres-Voraciously-to-Target-of-Rapamycin-2 Protein 1 (Avo1) inSaccharomyces cerevisiae

(2011) Hsien-Ching Liao et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Requirements of Slm proteins for proper eisosome organization, endocytic trafficking and recycling in the yeast Saccharomyces cerevisiae

(2011) Chitra Kamble et al.   JOURNAL OF BIOSCIENCES

Eisosome proteins assemble into a membrane scaffold

(2011) Lena Karotki et al.   JOURNAL OF CELL BIOLOGY

The eisosome core is composed of BAR domain proteins

(2011) Agustina Olivera-Couto et al.   MOLECULAR BIOLOGY OF THE CELL

Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae

(2011) F. M. Roelants et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The reverse, but coordinated, roles of Tor2 (TORC1) and Tor1 (TORC2) kinases for growth, cell cycle and separase-mediated mitosis in Schizosaccharomyces pombe

(2011) N. Ikai et al.   Open Biology

ABP1 Mediates Auxin Inhibition of Clathrin-Dependent Endocytosis in Arabidopsis

(2010) Stéphanie Robert et al.   CELL

A systematic screen for protein–lipid interactions in Saccharomyces cerevisiae

(2010) Oriol Gallego et al.   Molecular Systems Biology

Pheromone-induced anisotropy in yeast plasma membrane phosphatidylinositol-4,5-bisphosphate distribution is required for MAPK signaling

(2010) L. S. Garrenton et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TORC2 Plasma Membrane Localization Is Essential for Cell Viability and Restricted to a Distinct Domain

(2009) Doris Berchtold et al.   MOLECULAR BIOLOGY OF THE CELL

An auxin-based degron system for the rapid depletion of proteins in nonplant cells

(2009) Kohei Nishimura et al.   NATURE METHODS

Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE

(2009) Eiji Kinoshita et al.   Nature Protocols

Regulation of Ceramide Biosynthesis by TOR Complex 2

(2008) Sofia Aronova et al.   Cell Metabolism

Involvement ofSaccharomyces cerevisiaeAvo3p/Tsc11p in Maintaining TOR Complex 2 Integrity and Coupling to Downstream Signaling

(2008) Hsiang-Ling Ho et al.   EUKARYOTIC CELL

TOR1 and TOR2 Have Distinct Locations in Live Cells

(2008) Thomas W. Sturgill et al.   EUKARYOTIC CELL

Highly inclined thin illumination enables clear single-molecule imaging in cells

(2008) Makio Tokunaga et al.   NATURE METHODS

Membrane recognition by phospholipid-binding domains

(2008) Mark A. Lemmon   NATURE REVIEWS MOLECULAR CELL BIOLOGY