Article
Food Science & Technology
Xiangpeng Han, Qingying Chen, Xingguo Zhang, Xiaolan Chen, Dongsheng Luo, Qingping Zhong
Summary: This study found that Lactiplantibacillus plantarum 2057 can effectively inhibit the formation of Vibrio parahaemolyticus biofilm and interfere with its adhesion ability. In addition, the extract of L. plantarum Z057 significantly reduces the biofilm biomass, metabolic activity, and various important components of V. parahaemolyticus. These findings suggest that L. plantarum 2057 could be a biocontrol strategy alternative to chemical methods against foodborne pathogens.
Article
Biochemistry & Molecular Biology
Fuwen Liu, Fei Wang, Yixuan Yuan, Xiaoran Li, Xiaojun Zhong, Menghua Yang
Summary: Vibrio parahaemolyticus is a significant food-borne pathogen. The study showed that the three signal synthases, CqsA(vp), LuxM(vp), and LuxS(vp), are essential for activating quorum sensing and regulating swarming in V. parahaemolyticus. The absence of these synthases leads to swarming defects.
MOLECULAR MICROBIOLOGY
(2023)
Article
Microbiology
Jessica G. Tague, Abish Regmi, Gwendolyn J. Gregory, E. Fidelma Boyd
Summary: Factor for inversion stimulation (Fis) positively regulates the expression of qrr sRNAs in V. parahaemolyticus, controlling swarming motility indirectly through the QS pathway and directly through the surface sensing pathway.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Qiuyu Wang, Pengfei Wang, Pingping Liu, Jie Ou
Summary: The study found significant differences in the expression levels of 956 genes in biofilms compared with planktonic cells, suggesting that two-component regulatory system (TCS) and quorum sensing (QS) regulate V. parahaemolyticus biofilm formation by affecting important factors such as flagellar motility, Extracellular polymeric substance (EPS) secretion, tripartite ATP-independent (TRAP) transport system, and ATP binding cassette (ABC) transport system. This work in transcriptomics provides a basis for future studies examining the complex network systems that regulate bacterial biofilm formation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Kyle D. Brumfield, Arlene J. Chen, Mayank Gangwar, Moiz Usmani, Nur A. Hasan, Antarpreet S. Jutla, Anwar Huq, Rita R. Colwell
Summary: The incidence of vibriosis is increasing globally due to changing climatic conditions that promote the growth of pathogenic Vibrio species. Environmental factors, such as temperature, salinity, total chlorophyll a, dissolved oxygen, and pH, were found to impact the occurrence of pathogenic Vibrio species. Long-term monitoring revealed an increase in Vibrio populations in the Chesapeake Bay region. The findings highlight the importance of predicting and managing Vibrio-related diseases in the face of climate change.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Ce Zhang, Min Liu, Ying Wu, Xixi Li, Chen Zhang, Douglas R. Call, Ming Liu, Zhe Zhao
Summary: In Vibrio parahaemolyticus, the expression of T3SS1 is regulated by a complex quorum sensing phosphorelay circuit involving the hybrid histidine kinase ArcB, sensor kinase LuxQ, and response regulator LuxO. The study shows that LuxQ can directly interact with ArcB under high oxygen conditions, leading to phosphorylation of LuxO and activation of T3SS1 expression. Under hypoxic conditions, ArcB autophosphorylates and phosphorylates ArcA, but ArcA does not participate in regulating T3SS1 expression.
Article
Microbiology
Xiaojun Zhong, Ranran Lu, Fuwen Liu, Jinjie Ye, Junyang Zhao, Fei Wang, Menghua Yang
Summary: This study identified a novel LuxR family regulator, RobA, in Vibrio parahaemolyticus, which influences bacterial growth and biofilm formation, and integrates into the quorum sensing circuit. RobA was found to regulate the expression of exopolysaccharides synthesis cluster and interact with other regulators to construct a complex regulatory network of biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Genetics & Heredity
Junfang Sun, Xue Li, Yue Qiu, Xingfan Xue, Miaomiao Zhang, Wenhui Yang, Dongsheng Zhou, Lingfei Hu, Renfei Lu, Yiquan Zhang
Summary: Vibrio parahaemolyticus produces two types of IV pili, MSHA and ChiRP, both of which are required for biofilm formation and pathogen persistence. In this study, it was shown that the master quorum sensing regulators, AphA and OpaR, oppositely regulated the transcription of the pilin encoding gene mshA1. This regulation resulted in a gradual increase in mshA1 expression levels from low to high cell density, demonstrating a mechanism for controlling biofilm formation.
Article
Genetics & Heredity
Olivia Duddy, Justin Silpe, Chenyi Fei, Bonnie Bassler
Summary: Quorum sensing (QS) is a chemical communication process used by bacteria to monitor population density and coordinate collective behaviors. In this study, researchers found that a bacterial virus, phage VP882, manipulates the host QS system through a QS receptor-transcription factor called VqmA. Dysfunction of the QS systems in Vibrio parahaemolyticus strain O3:K6 882 was caused by mutations in both vqmR-vqmA and luxO genes. Repairing these defects promoted the activation of the phage VP882 lytic gene expression and led to increased lysis of the bacterial host. The study suggests that maintaining a low-cell density QS state protects the bacterial host from phage-mediated lysis.
Article
Food Science & Technology
Yibin Xu, Yuanyuan Wang, Xiaoqing Ding, Jiangshui Wang, Xiuan Zhan
Summary: This study demonstrates the antibacterial and antibiofilm properties of reuterin derived from L. reuteri strains. Reuterin exerts its bactericidal activity through ROS production. Furthermore, co-cultures with glycerol enhance the inhibitory effects of LR 21 on Cp 13124, reducing bacterial count and the production of virulence factors.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Immunology
Ezhaveni Sathiyamoorthi, Jin-Hyung Lee, Yulong Tan, Jintae Lee
Summary: This study investigated the antibacterial and antivirulence effects of chromones and their derivatives against Vibrio parahaemolyticus and Vibrio harveyi. 6-Bromo-3-formylchromone (6B3FC) and 6-chloro-3-formylchromone (6C3FC) were found to be active against these bacteria and inhibited biofilm formation. 6B3FC also showed inhibition of gene expression associated with quorum sensing and biofilm formation in V. parahaemolyticus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Yuehua Li, Junxiang Yan, Jinghao Li, Xinke Xue, Ying Wang, Boyang Cao
Summary: This study identified a new quorum sensing regulator, LuxT, which directly inhibits hapR expression, leading to increased virulence in Vibrio cholerae. It was also found that the expression of LuxT is regulated by the two-component system ArcB/ArcA. These findings reveal a novel cell density-mediated virulence regulation pathway and contribute to a better understanding of the pathogenic mechanisms of V. cholerae.
Article
Microbiology
Zhidong Zhang, Yang Sun, Yuanyang Yi, Xiaoyu Bai, Liying Zhu, Jing Zhu, Meiying Gu, Yanlei Zhu, Ling Jiang
Summary: A strain of Streptomyces with quorum-sensing inhibitory activity was isolated from the Kumutag Desert in Xinjiang, and it effectively inhibited biofilm formation and virulence factor production in bacteria such as Pseudomonas aeruginosa.
Article
Biotechnology & Applied Microbiology
Haixi Lu, Yang Sun, Xiaodi Wang, Zhong Lu, Junli Zhu
Summary: This study investigates the inhibitory effects of salinity and citral on the biofilm formation of Vibrio parahaemolyticus (VP) and its potential mechanism. The results show that combining high salinity with citral significantly reduces the biofilm formation of VP and alters the gene expression patterns related to biofilm development.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Yiquan Zhang, Xingfan Xue, Fengjun Sun, Xue Li, Miaomiao Zhang, Qimin Wu, Tingting Zhang, Xi Luo, Renfei Lu
Summary: This study reveals that AphA indirectly activates the transcription of vpa0607-qsvR, while OpaR and QsvR directly repress the transcription of vpa0607-qsvR, resulting in the highest expression levels at low cell density.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Yi Sun, Xueyan Hu, Du Guo, Chao Shi, Chunling Zhang, Xiaoli Peng, Hua Yang, Xiaodong Xia
MICROBIAL DRUG RESISTANCE
(2019)
Article
Microbiology
Yichen Bai, Haibo Yu, Du Guo, Shengyi Fei, Chao Shi
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Microbiology
Yi Sun, Du Guo, Zi Hua, Huihui Sun, Zhanwen Zheng, Xiaodong Xia, Chao Shi
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Food Science & Technology
Chao Shi, Tong Jin, Du Guo, Wenting Zhang, Baowei Yang, Dongfang Su, Xiaodong Xia
FOODBORNE PATHOGENS AND DISEASE
(2020)
Article
Biotechnology & Applied Microbiology
Du Guo, Shuo Wang, Jiahui Li, Fangting Bai, Yanpeng Yang, Yunfeng Xu, Sen Liang, Xiaodong Xia, Xin Wang, Chao Shi
Article
Biochemistry & Molecular Biology
Du Guo, Fangting Bai, Xiangjun Zhan, Wenting Zhang, Tong Jin, Yutang Wang, Xiaodong Xia, Chao Shi
Summary: Citral protects Caco-2 cells by inhibiting the adhesion and invasion of C. sakazakii and suppressing inflammatory response and apoptosis. It has the potential to prevent inflammation and mitigate the effects of C. sakazakii in Caco-2 cells.
Article
Food Science & Technology
Du Guo, Yichen Bai, Shengyi Fei, Yanpeng Yang, Jiahui Li, Baowei Yang, Xin Lu, Xiaodong Xia, Chao Shi
Summary: The research findings suggest that 405 nm LED-illumination treatment reduces the resistance of S. Typhimurium to environmental stress and downregulates the transcription levels of environmental tolerance-related genes. These results provide potential application prospects for using LED-illumination technology to control pathogens in food processing and reduce infections and diseases related to S. Typhimurium.
Article
Food Science & Technology
Fangting Bai, Du Guo, Yueyi Wang, Shanbo Zhang, Jiahui Li, Kangkang Zhi, Chao Shi, Xiaodong Xia
Summary: This study investigated the combined bactericidal effect of nisin and thymoquinone on Listeria monocytogenes in Tryptone Soy Broth and sterilized milk. The combination led to a greater reduction in bacterial number and appeared to cause early injury to the bacteria. Nisin and thymoquinone acted on the bacteria in different growth phases and demonstrated a reversible hydrogen-bonding interaction.
