Protein A chromatography: Challenges and progress in the purification of monoclonal antibodies

Protein A and protein A/G coupled magnetic SiO 2 microspheres for affinity purification of immunoglobulin G

(2018) Kouroush Salimi et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Evaluation of recent Protein A stationary phase innovations for capture of biotherapeutics

(2018) Timothy M. Pabst et al.   JOURNAL OF CHROMATOGRAPHY A

Chemical modification of protein a chromatography ligands with polyethylene glycol. II: Effects on resin robustness and process selectivity

(2018) Justin Weinberg et al.   JOURNAL OF CHROMATOGRAPHY A

An enhanced regeneration strategy to improve microbial control and prolong resin lifetime for Protein A resin in large-scale monoclonal antibody (mAb) purification

(2018) Tianyi Zhou et al.   JOURNAL OF BIOTECHNOLOGY

An engineered Staphylococcal Protein A based ligand: Production, characterization and potential application for the capture of Immunoglobulin and Fc-fusion proteins

(2018) Martin Kangwa et al.   PROTEIN EXPRESSION AND PURIFICATION

Feasibility of using continuous chromatography in downstream processing: Comparison of costs and product quality for a hybrid process vs. a conventional batch process

(2017) Ozan Ötes et al.   JOURNAL OF BIOTECHNOLOGY

Definition and dynamic control of a continuous chromatography process independent of cell culture titer and impurities

(2017) Rebecca A. Chmielowski et al.   JOURNAL OF CHROMATOGRAPHY A

Determination of isoelectric points and relative charge variants of 23 therapeutic monoclonal antibodies

(2017) Alexandre Goyon et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Purification of monoclonal antibodies entirely in flow-through mode

(2017) Tsuyoshi Yamada et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

The role of more than 40 years of improvement in protein A chromatography in the growth of the therapeutic antibody industry

(2016) Glen R. Bolton et al.   BIOTECHNOLOGY PROGRESS

Characterization of methacrylate chromatographic monoliths bearing affinity ligands

(2016) Urh Černigoj et al.   JOURNAL OF CHROMATOGRAPHY A

Clearance of the rodent retrovirus, XMuLV, by protein A chromatography

(2015) Julia Bach et al.   BIOTECHNOLOGY AND BIOENGINEERING

The use of combinations of monoclonal antibodies in clinical oncology

(2015) Linda M. Henricks et al.   CANCER TREATMENT REVIEWS

A straightforward methodology for designing continuous monoclonal antibody capture multi-column chromatography processes

(2015) Xhorxhi Gjoka et al.   JOURNAL OF CHROMATOGRAPHY A

Preparation and characterization of novel IgG affinity resin coupling anti-Fc camelid single-domain antibodies

(2015) Zhui Tu et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Evaluation of a novel methacrylate-based protein a resin for the purification of immunoglobulins and Fc-fusion proteins

(2014) Tyler R. McCaw et al.   BIOTECHNOLOGY PROGRESS

Toward improving selectivity in affinity chromatography with PEGylated affinity ligands: The performance of PEGylated protein A

(2014) José González-Valdez et al.   BIOTECHNOLOGY PROGRESS

Virus elimination during the purification of monoclonal antibodies by column chromatography and additional steps

(2014) Peter L. Roberts   BIOTECHNOLOGY PROGRESS

Separation of human immunoglobulin G subclasses on a protein A monolith column

(2014) Pelin Leblebici et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Structural evaluation of an alternative Protein A biomimetic ligand for antibody purification

(2014) Telma Barroso et al.   JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN

Quality by design approach for viral clearance by protein a chromatography

(2013) Min Zhang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Separation of nonfucosylated antibodies with immobilized FcγRIII receptors

(2013) Glen R. Bolton et al.   BIOTECHNOLOGY PROGRESS

Design, synthesis and application of benzyl-sulfonate biomimetic affinity adsorbents for monoclonal antibody purification from transgenic corn

(2013) Anastasios Maltezos et al.   JOURNAL OF MOLECULAR RECOGNITION

Affinity monolith chromatography: a review of principles and recent analytical applications

(2012) Erika L. Pfaunmiller et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Predicting protein dynamic binding capacity from batch adsorption tests

(2012) Giorgio Carta   Biotechnology Journal

Process Development for Antibody Purification from Tobacco by Protein A Affinity Chromatography

(2011) C. Hey et al.   CHEMICAL ENGINEERING & TECHNOLOGY

Performance of hexamer peptide ligands for affinity purification of immunoglobulin G from commercial cell culture media

(2010) Amith D. Naik et al.   JOURNAL OF CHROMATOGRAPHY A

Industrialization of mAb production technology: The bioprocessing industry at a crossroads

(2010) Brian Kelley   mAbs

Development of a high throughput protein a well-plate purification method for monoclonal antibodies

(2009) Jennifer Hopp et al.   BIOTECHNOLOGY PROGRESS

Predictive chromatographic simulations for the optimization of recovery and aggregate clearance during the capture of monoclonal antibodies

(2009) Mark Teeters et al.   JOURNAL OF CHROMATOGRAPHY A

Virus inactivation by solvent/detergent treatment using Triton X-100 in a high purity factor VIII

(2008) Peter L. Roberts   BIOLOGICALS

Performance of a New Protein A Affinity Membrane for the Primary Recovery of Antibodies

(2008) C. Boi et al.   BIOTECHNOLOGY PROGRESS

High-performance monolith affinity chromatography for fast quantitation of immunoglobulin G

(2008) Anne Tscheliessnig et al.   JOURNAL OF CHROMATOGRAPHY A

Purification of human immunoglobulin G via Fc-specific small peptide ligand affinity chromatography

(2008) Haiou Yang et al.   JOURNAL OF CHROMATOGRAPHY A