Review
Biochemical Research Methods
Saumen Poddar, Sadia Sharmeen, David S. Hage
Summary: Affinity monolith chromatography (AMC) utilizes a monolithic support with a biological ligand to isolate, enrich, or detect target analytes. The specific interactions exhibited by the binding agents make AMC attractive for separating a wide range of compounds. Applications include bioaffinity chromatography, immunoaffinity chromatography, and potential uses in chiral separations and biointeraction studies.
Article
Biochemical Research Methods
Andrea Gottardini, Vincent Dugas, Claire Demesmay
Summary: Affinity chromatography is a powerful technique for identifying and quantifying weak ligand-protein interactions. A novel approach of protein immobilization based on streptavidin-biotin interaction was presented in this study to improve bulk density of active protein, expanding the affinity range of the technique to the millimolar range.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Frederic Jeanroy, Claire Demesmay, Vincent Dugas
Summary: This work explores the capability of antithrombin III-functionalized capillary monolithic columns to selectively capture. release, and detect high affinity binders of antithrombin III. The optimized miniaturized antithrombin III monolithic affinity columns were coupled with MS detection and demonstrated to allow the selective capture and elution of high affinity binders, with a limit of detection of 1 pmol.
Article
Chemistry, Analytical
Zhang-Na Qin, Xi Chen, Qiong-Wei Yu, Jun Ding, Yu-Qi Feng
Summary: Protein phosphorylation is crucial for biological functions, and in this study, a method for phosphopeptide enrichment using an arsenate functionalized monolithic column coupled with mass spectrometry analysis was developed successfully. The method exhibited high selectivity and satisfactory performance in analyzing phosphopeptides from complex biological samples such as human serum and rat brain proteins.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Applied
Raquel Portela, Jakob Maximilian Marinkovic, Morten Logemann, Markus Schoerner, Nanette Zahrtman, Esra Eray, Marco Haumann, Eduardo J. Garcia-Suarez, Matthias Wessling, Pedro Avila, Anders Riisager, Rasmus Fehrmann
Summary: Monolithic support materials with the mechanical resistance and thermal conductivity of SiC, as well as tunable surface chemistry and textural properties, have been developed for use in catalytic membrane reactors. The skin applied onto the external wall and the infiltration of metal oxide nanoparticles into macropores allow for the immobilization of liquid phase catalysts. The resulting multimodal distribution of pore sizes can be tuned by various factors such as particle sizes, wash-coats, and calcination temperature.
Article
Biochemical Research Methods
Fatma Cambay Kuban, Ilkay Kocer, Cigdem Kip, Eda Celik, Ali Tuncel
Summary: A new capillary monolithic stationary phase was synthesized for the purification of histidine tagged proteins by immobilized metal affinity micro-chromatography (mu-IMAC). The monolith was obtained by thiol-methacrylate polymerization using methacryl substituted-polyhedral oligomeric silsesquioxane (POSS-MA) and mercaptosuccinic acid (MSA) as the thiol functionalized agent in a fused silica capillary tubing. Ni(II) cations were immobilized onto the porous monolith via metal-chelate complex formation with double carboxyl functionality of bound MSA segments. His-GFP was successfully isolated with high yield and purity using the Ni(II)@MSA@poly(POSS-MA) capillary monolith. The monolith showed stable performance over consecutive His-GFP purifications.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Tingting Zhao, Min Zhang, Qi Peng, Xucong Lin, Zenghong Xie
Summary: A facile protocol of DNA adsorption on UV-initiated polymerization supports was proposed for fabricating aptamer-based affinity monolithic column, achieving ultra-high aptamer coverage density and highly specific online recognition performance. This approach avoids limitations of traditional biological or covalent modification strategies.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Ling Yang, Ting Huang, Shuqiang Wang, Jingpu Tang, Chenchen Lin, Xiaoping Wu
Summary: This study describes a histidine-tagged hexapeptide functionalized monolithic material for efficient capillary microextraction (CME) of microcystins (MCs) from environmental waters. The affinity sorbent was prepared by functionalizing a Cu2+ modified polymer monolith with a histidine tag peptide via chelation reaction. This novel biomimetic sorbent showed high extraction efficiency and repeatability for ultra-trace pollutants in environment waters.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Akhmad Sabarudin, Shin Shu, Kazuhiro Yamamoto, Tomonari Umemura
Summary: In this study, efficient flow-through microreactors for high-throughput organic synthesis were developed by chemically immobilizing different base catalysts onto ligand-modified poly(GMA-co-EDMA) monoliths. The microreactors showed excellent performance in Suzuki-Miyaura cross-coupling reactions, with the poly(GMA-co-EDMA) monolith modified with 5-amino-1,10-phenanthroline as a binding site for the palladium catalyst providing the best results.
Article
Biochemistry & Molecular Biology
Julia Neumair, Claudia D'Ercole, Matteo De March, Martin Elsner, Michael Seidel, Ario de Marco
Summary: In this study, a new affinity chromatography platform composed of a macroporous epoxy monolith matrix functionalized with anti-CD63 nanobodies was developed. The platform successfully captured and released integral EVs from urine samples with negligible unspecific binding for circulating proteins. Size discrimination of eluted EVs was achieved by different elution approaches. The use of inexpensive recombinant nanobodies and scalable monolith materials make this platform feasible and affordable for large-scale production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Chemical
Nicolas Urtasun, Arn Mignon, Lucas M. Martinez-Alvarez, Maria F. Baieli, Daniela B. Hirsch, Osvaldo Cascone, Peter Dubruel, Federico J. Wolman
Summary: Lactoperoxidase (LP) and lactoferrin (LF) are two proteins in dairy whey with various beneficial properties. Traditional purification methods require pre-treatment steps, but the novel support material CMS-Orange R-HE shows promising adsorption capabilities. Through a one-step adsorption and two-step elution process, LP and LF can be obtained with high yields, purity, and minimal cross-contamination.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Tingting Zhao, Xinyue Ding, Yiqiong Chen, Chenchen Lin, Guomin Qi, Xucong Lin, Zenghong Xie
Summary: A new and facile strategy was developed to prepare aptamer-based hybrid affinity monolith in one-pot at room temperature, achieving a more efficient and rapid method. By optimizing the recipe for photo-initiated polymerization, the monolith showed a more homogeneous skeleton structure, higher aptamer reaction efficiency, and better specific selectivity to OTA.
ANALYTICA CHIMICA ACTA
(2021)
Article
Polymer Science
Hande Acikalin, Pradeep K. R. Panyam, Abdul Wasif Shaikh, Dongren Wang, Shravan R. Kousik, Petia Atanasova, Michael R. Buchmeiser
Summary: Non-porous polyurethane-based monoliths with low specific surface areas and pore volumes are prepared under solvent-induced phase separation conditions. Mesoporosity can be introduced by varying the monomers and solvents. The monoliths are functionalized with quaternary ammonium groups and used to immobilize an ionic liquid containing an ionic Rh-catalyst for hydrosilylation reactions. The confined small mesopores contribute to the significant increase in Z-selectivity.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Jacob H. Forstater, Shane T. Grosser
Summary: Immobilization technology plays a significant role in commodity chemical synthesis and pharmaceutical synthesis. This study presents a data-rich approach that automates the screening and development of affinity-based enzyme immobilizations, enabling real-time process characterization and rapid decision making. The developed methodology reduces the time and resources required for immobilization process development and optimization.
REACTION CHEMISTRY & ENGINEERING
(2022)
Article
Engineering, Chemical
Wei Gao, Feng Zhang, Sen Zhang, Jia-yuan Li, Hong-zhen Lian
Summary: High selective enrichment of low abundance phosphopeptides from complex biological samples is essential for in-depth phosphoproteomics. A magnetic material called mMOF-FBP-Ti4+, fabricated by grafting UiO-66-NH2 metal-organic framework onto the surface of Fe3O4 and immobilizing Ti(IV) with fructose 1,6-bisphosphate, exhibited favorable properties for phosphopeptide enrichment. The material demonstrated strong affinities through metal oxide affinity chromatography and immobilized metal affinity chromatography, as well as improved hydrophilicity for enhanced hydrophilic chromatography interaction. The mMOF-FBP-Ti4+ also yielded low detection limit, high selectivity, good reusability, and high postenrichment recovery towards phosphopeptides, making it a promising tool for efficient enrichment in complex biological samples.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)