Article
Biotechnology & Applied Microbiology
Dev Kumar Verma, Luca Peruzza, Franziska Trusch, Manoj Kumar Yadav, Ravindra, Sergei V. Shubin, Kenton L. Morgan, Vindhya Mohindra, Chris Hauton, Pieter van West, P. K. Pradhan, Neeraj Sood
Summary: The study revealed that common carp exhibit resistance mechanisms against Aphanomyces invadans infection, involving efficient processing and presentation of antigens, enhanced phagocytosis, recognition of pathogen-associated molecular patterns, and increased leukocyte recruitment.
Article
Multidisciplinary Sciences
Diem Tho Ho, Nameun Kim, Yoonhang Lee, Dongbin Yun, MinJi Sung, El-Matbouli Mansour, P. K. Pradhan, Neeraj Sood, Wi-Sik Kim, Chan-Il Park, Ki Hong Kim, Do-Hyung Kim
Summary: In this study, a novel TaqMan probe-based qPCR method was developed for sensitive and quantitative detection of Aphanomyces invadans. The assay showed high accuracy and applicability, making it important for monitoring pathogens in aquatic environments.
Article
Fisheries
Bini C. Das, Divya Haridas, Nithin Jacob, Arunendhu Ramakrishnan, Anvar P. H. Ali, Rejish V. J. Kumar, Devika Pillai
Summary: Large-scale fish mortality occurred in fish farms across several parts of Kerala in August 2018 and 2019, following heavy rainfall. The deaths were mainly caused by the highly infectious disease epizootic ulcerative syndrome (EUS), with severe infections observed in freshwater and brackish water environments affecting fish species such as snakeheads and mullet. The severity of the outbreak was linked to sudden changes in water quality associated with the floods, including lower water temperature, pH, total alkalinity, and total hardness.
DISEASES OF AQUATIC ORGANISMS
(2021)
Article
Fisheries
Yang He, Wei Fan, Jian Su, Jun Wang, Yonghong Liu, Chuanjie Qin, Yuanchao Zou, Xiaotao Shi, Jin Wang
Summary: A suspected epizootic ulcerative syndrome outbreak occurred in farmed albino-type Northern Snakeheads in Jinming farm, southwest China. Aphanomyces invadans was identified as the causative agent based on laboratory examinations and histopathological changes.
NORTH AMERICAN JOURNAL OF AQUACULTURE
(2022)
Article
Fisheries
Samayanpaulraj Vignesh, Govindaraj Krishnaveni, J. C. Walter Devaa, Sivaramapillai Muthukumar, Ramesh Uthandakalaipandian
Summary: This study investigated the pathophysiological effects of Aeromonas hydrophila on snakehead murrel fish under sublethal conditions. Results showed higher bacterial load in muscle tissue and lower load in the kidney tissue of the infected group. Hematological parameters and serum protein levels were significantly decreased, while liver enzyme markers were elevated. Antioxidant genes were highly expressed in the liver tissue. Immune regulatory gene IL-8 was downregulated, while TGF-beta was upregulated. Various pathological changes, including swimming imbalance and fin injury, were observed during A. hydrophila infection.
AQUACULTURE INTERNATIONAL
(2022)
Article
Fisheries
Rakhi Kumari, P. P. Srivastava, Kedar N. Mohanta, Paramananda Das, Rajesh Kumar, Lakshman Sahoo, Prakash Sharma, Gopal Krishna, Anirban Paul, G. M. Siddaiah
Summary: The pattern of gene expression and the activity of three proteases were investigated in larvae and juveniles of striped murrel. The study found that the expression levels and activities of trypsin, chymotrypsin, and pepsin varied during the growth stages, suggesting the optimal weaning period for larvae/juveniles of C. striata.
Article
Multidisciplinary Sciences
Shizhe Yu, Haoren Wang, Lingpeng Yang, Yingxue Yan, Qiang Cai, Duo Ma, Long Jiang, Zehai Gao, Zhiyong Yu, Zongping Xia
Summary: The comprehensive study of the spatial-cellular anatomy of the human liver is crucial for understanding the cellular origins of liver disease. In this study, spatial transcriptomics were conducted on normal human liver tissue sections, providing detailed transcriptional information on liver zonation. A total of 6581 high-quality spots from normal livers were analyzed, mainly consisting of hepatocytes, which were classified into four sub-groups. These data serve as a reliable reference for studying the spatial heterogeneity of liver lobules.
Article
Multidisciplinary Sciences
Sanjeeva Metikala, Satish Casie Chetty, Saulius Sumanas
Summary: In this study, single-cell RNA-seq was used to analyze over 20,000 cells disaggregated from the trunk region of zebrafish embryos at the 30 hpf stage, identifying and annotating transcriptional signatures of 27 different cell types and subtypes. This dataset will serve as a valuable resource for researchers in developmental and cellular biology, aiding in the understanding of molecular mechanisms that regulate cell lineage choices during development.
Article
Fisheries
Hao Yang, Xiaoting Feng, Xiao Tu, Fangyuan Liu, Ruixue Hu, Zemao Gu
Summary: Epizootic ulcerative syndrome (EUS) is an emerging disease that causes high morbidity and mortality in yellow catfish. The causative agent for EUS in T. fulvifraco is not fully determined. An epidemiological investigation in Hubei province, China, revealed that EUS outbreaks mainly occur in March and April when the water temperature ranges from 13°C to 20°C. Diseased T. fulvifraco exhibit circular-hole shaped ulcers on the skin, and ciliate infections are commonly associated with early EUS. Further diagnoses showed that ciliated parasites, Ichthyophthirius multifiliis and Epistylis horizontalis, heavily infected the specimens, causing severe damages on the skin and gills. In contrast, bacterial infection with Aeromonas versonii was found in only 12.5% of the specimens. These findings suggest that ciliated parasites are the primary agents for early EUS in T. fulvifraco.
Article
Biotechnology & Applied Microbiology
David W. McKellar, Madhav Mantri, Meleana M. Hinchman, John S. L. Parker, Praveen Sethupathy, Benjamin D. Cosgrove, Iwijn De Vlaminck
Summary: Spatial transcriptomics allows us to study the spatial context of gene expression. Current methods are limited to detecting a subset of RNA transcripts. By using enzymatic in situ polyadenylation of RNA, we are able to detect the full spectrum of RNAs, expanding the scope of research. Applying this approach, we studied skeletal muscle regeneration and viral-induced myocarditis, and made new discoveries.
NATURE BIOTECHNOLOGY
(2023)
Article
Biology
Vinay S. Swamy, Temesgen D. Fufa, Robert B. Hufnagel, David M. McGaughey
Summary: A lightweight R package, scPOP, was developed to create an ocular single-cell transcriptome meta-atlas by balancing batch merging and cell type/cluster purity. This provides an efficient model for creating meta-atlases for tissues and cells of interest.
Article
Immunology
Nikita R. Raje, Janelle R. Noel-MacDonnell, Katherine A. Shortt, Nicole M. Gigliotti, Marcia A. Chan, Daniel P. Heruth
Summary: The phenotypic variations of chromosome 22q11.2 deletion syndrome (22qDS) are not well explained. This study investigated gene expression in T cells of individuals with and without 22qDS, and found differentially expressed genes associated with T cell counts and markers. This suggests an important role of T cells in defective communication in 22qDS.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiaoyu Song, Jiayi Ji, Joseph H. Rothstein, Stacey E. Alexeeff, Lori C. Sakoda, Adriana Sistig, Ninah Achacoso, Eric Jorgenson, Alice S. Whittemore, Robert J. Klein, Laurel A. Habel, Pei Wang, Weiva Sieh
Summary: Researchers have developed a new cell-type-aware transcriptome-wide association study approach to predict cell-type level gene expression and identify disease-associated genes. This approach provides insights into the genetic and cellular etiology of diseases such as breast cancer.
NATURE COMMUNICATIONS
(2023)
Article
Horticulture
Xiaonan Dai, Qingyu Li, Fudong Jiang, Zhizhong Song, Xiaoli Tang, Shuqi Su, Ruotong Yao, Haiying Yang, Yanqing Yang, Hongxia Zhang, Jianzhao Li
Summary: This study clarified the mechanism of anthocyanin accumulation in "Red Bartlett" pear through transcriptome analysis, and identified the genes and transcription factors associated with anthocyanin biosynthesis and regulation.
SCIENTIA HORTICULTURAE
(2022)
Article
Fisheries
Ljudevit Luka Bostjancic, Caterina Francesconi, Lena Bonassin, Sandra Hudina, Romana Gracan, Ivana Maguire, Christelle Rutz, Ana Beck, Ana Dobrovic, Odile Lecompte, Kathrin Theissinger
Summary: Alien invasive species are one of the main drivers of biodiversity loss in freshwater ecosystems. In Europe, the pathogen Aphanomyces astaci is considered one of the most problematic invasive species, as its introduction has severely reduced European freshwater crayfish stocks, particularly the noble crayfish population. However, the temporal dynamics of the noble crayfish immune response during Ap. astaci infection remains poorly understood.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ajay Guru, Tamilvelan Manjunathan, Gokul Sudhakaran, Annie Juliet, Pushparathinam Gopinath, Jesu Arockiaraj
Summary: Antioxidant natural products and their analogs, especially phenolic compounds, have diverse biological activities, including anti-inflammatory, antioxidant, and anticancer activities. Ginger, widely used for its beneficial effects, contains phenolic antioxidants, such as 6-gingerol. However, the molecular mechanism of synthetically synthesized 6-gingerdione (compound 1) from 6-gingerol was unknown. In this study, compound 1 and methylated 6-gingerdione (compound 2) were obtained and their antioxidant and anti-inflammatory activities were analyzed. Compound 1 showed stronger antioxidant and anti-inflammatory activities compared to compound 2. Additionally, compound 1 protected liver injury in zebrafish larvae induced by 2% EtOH.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Chemistry, Applied
Arunkumar Kathiravan, Tamilvelan Manjunathan, Marappan Velusamy, Ajay Guru, Jesu Arockiaraj, Mariadoss Asha Jhonsi, Pushparathinam Gopinath
Summary: A highly sensitive probe KG1 was developed to detect hypochlorous acid in water. The KG1 aggregates showed excellent fluorescence and were characterized by HRTEM and photophysical investigations. The KG1 aggregates have a spherical shape with an average size of 5 nm, and they have a high fluorescence lifetime and a quantum yield of 46%, making them suitable for bio-imaging and sensing applications. The probe KG1 can detect hypochlorous acid without interference and has a low detection limit of 667 pM, making it a prospective tool for investigating the biological mechanisms of hypochlorous acid in living systems.
Article
Multidisciplinary Sciences
Purabi Sarkar, Stefi Raju, Manikandan Velayutham, Ajay Guru, Mukesh Pasupuleti, Ebtesam M. Al Olayan, Amy F. Boushra, Annie Juliet, Jesu Arockiaraj
Summary: Diabetes-induced oxidative stress can damage organs and lead to various problems. Antioxidant therapy, such as GP13 peptide, shows promise in preventing diabetes and its complications. This study investigated the activity of GP13 peptide in an in-vitro diabetic model.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Pharmacology & Pharmacy
B. Haridevamuthu, Boopathi Seenivasan, P. Snega Priya, Subramani Muthuraman, Rajendran Saravana Kumar, K. Manikandan, Bader O. Almutairi, Mikhlid H. Almutairi, Selvaraj Arokiyaraj, Pushparathinam Gopinath, Jesu Arockiaraj
Summary: Non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) are common liver diseases caused by a western diet high in fats, which induce inflammation and impair hepatocyte function. Currently, there is a lack of pharmacological drugs for treating NAFLD/NASH. However, long pepper contains a bioactive compound called Piperlongumine (PL) that has potential antioxidant properties. In this study, demethylated dihydroxy piperlongumine (DHPL) was shown to have high antioxidant activity and was effective in reducing lipid accumulation and gene expression associated with NAFLD in a zebrafish model. DHPL may have therapeutic potential for halting the progression of NAFLD.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Geriatrics & Gerontology
Seenivasan Boopathi, R. M. Saravana Kumar, P. Snega Priya, B. Haridevamuthu, S. P. Ramya Ranjan Nayak, Laura Chulenbayeva, Kushugulova Almagul, Jesu Arockiaraj
Summary: Ageing is associated with changes in gut microbiota composition and functions, including a reduction in gut commensals and an increase in Enterobacteriaceae. Enterobacteriaceae are linked to all-cause mortality, metabolic diseases, telomere attrition, cellular senescence, inflammasome activation, impaired mitochondrial function, and multiorgan dysfunction. Targeting Enterobacteriaceae could potentially reduce inflammaging and enhance the longevity of older individuals.
EXPERIMENTAL GERONTOLOGY
(2023)
Article
Environmental Sciences
P. Snega Priya, Ajay Guru, Ramu Meenatchi, B. Haridevamuthu, Manikandan Velayutham, Boopathi Seenivasan, Raman Pachaiappan, Rajakrishnan Rajagopal, Palaniselvam Kuppusamy, Annie Juliet, Jesu Arockiaraj
Summary: This study evaluates the cytotoxic and teratogenic effects of methoxy phenols (MPs) generated by wildfire lignin on human embryonic kidney (HEK-293) cells and zebrafish embryos. The results indicate that MPs, particularly syringol, have toxic effects on both cells and embryos, including cell death, developmental abnormalities, and increased oxidative stress response. Therefore, syringol has the potential to pose serious health hazards in various living systems.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Ajay Guru, Ahmed Rady, Noura M. Darwish, Guilherme Malafaia, Selvaraj Arokiyaraj, Jesu Arockiaraj
Summary: In this study using zebrafish, combined exposure to microplastics (MPs) and abamectin (ABM) resulted in decreased survival rate, increased reactive oxygen species (ROS) generation, lipid peroxidation, apoptosis, impairment in antioxidant response, morphological changes in the eyes, and upregulated expression of specific apoptotic genes.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
S. P. Ramya Ranjan Nayak, L. S. Dhivya, R. Reshma, Bader O. Almutairi, Selvaraj Arokiyaraj, M. K. Kathiravan, Jesu Arockiaraj
Summary: This study investigates the anti-inflammatory effects of synthesized chalcone DKO7 in a zebrafish model of intestinal inflammation. The results demonstrate that DKO7 possesses anti-inflammatory and antioxidant properties, and protects zebrafish against oxidative stress and inflammation. The study provides evidence for the potential of DKO7 in alleviating DSS-induced gut inflammation.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Fisheries
Abhirami Chandran, P. Snega Priya, Ramu Meenatchi, S. Vaishnavi, V. Pavithra, Thipramalai Thankappan Ajith Kumar, Jesu Arockiaraj
Summary: Shrimp aquaculture is experiencing rapid growth, but is plagued by the severe threat of AHPND. Understanding shrimp immune responses and exploring new advancements in diagnostics, therapeutics, and research related to shrimp immunity is crucial in combating this devastating disease.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Editorial Material
Oncology
Muthupandian Saravanan, Jesu Arockiaraj
Article
Pharmacology & Pharmacy
Seenivasan Boopathi, P. Snega Priya, B. Haridevamuthu, S. P. Ramya Ranjan Nayak, Munisamy Chandrasekar, Jesu Arockiaraj, Ai-Qun Jia
Summary: Diverse microbial communities in different human body habitats known as the human microbiome play a crucial role in maintaining health. However, changes in the microbiome can lead to chronic low-grade inflammation and the translocation of pathogens and toxins to other organs, causing metabolic inflammation and non-communicable diseases. Recent studies have revealed that certain pathogens produce uraemic toxins that can enter the circulatory system and exacerbate various diseases. Enterobacteriaceae, a critical member of Proteobacteria, has been associated with non-communicable diseases and uraemic toxin production. This review provides an overview of Enterobacterial translocation and their metabolites' role in non-communicable diseases, which may aid in the discovery of biomarkers and therapeutic drugs.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Ajay Guru, Raghul Murugan, Jesu Arockiaraj
Summary: Pseudomonas is a group of bacteria that can cause various infections in weakened immune systems. In this study, an antimicrobial peptide called RW20 was derived from freshwater fish and tested against Pseudomonas aeruginosa. The results showed that RW20 had antibacterial properties and protected against Pseudomonas infection in both in vitro and in vivo experiments.
INTERNATIONAL MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Tamilvelan Manjunathan, Ajay Guru, B. Haridevamuthu, Rambabu Dandela, Jesu Arokiaraj, Pushparathinam Gopinath
Summary: A semisynthetic strategy was developed to synthesize novel analogs based on 6-gingerol, using simple and robust chemistries. These analogs demonstrated the ability to quench ROS and RNS in vitro, and alleviate oxidative stress induced by acrylamide in zebrafish larvae. They also restored locomotion behavior in zebrafish.
NEW JOURNAL OF CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Venkatesan Saravanan, Bharath Kumar Chagaleti, Pavithra Lakshmi Narayanan, Vijay Babu Anandan, Haritha Manoharan, G. V. Anjana, Ramalingam Peraman, S. Karthik Raja Namasivayam, M. Kavisri, Jesu Arockiaraj, Kathiravan Muthu Kumaradoss, Meivelu Moovendhan
Summary: SARS-CoV-2, the virus responsible for COVID-19, poses significant challenges to vaccine development due to viral mutation and other factors. Currently, many vaccines are in different stages of development, but considerations such as long-term immunity, safety, efficacy, stability, distribution, and cost are crucial.
CHEMICAL BIOLOGY & DRUG DESIGN
(2023)
Article
Engineering, Environmental
B. Haridevamuthu, Raghul Murugan, Boopathi Seenivasan, Ramu Meenatchi, Raman Pachaiappan, Bader O. Almutairi, Selvaraj Arokiyaraj, M. K. Kathiravan, Jesu Arockiaraj
Summary: Tartrazine is a commonly used azo dye that poses a threat to human health and the aquatic environment when used in excessive amounts. This study comprehensively evaluated the neurodevelopmental toxicity of Tartrazine and explored its underlying mechanisms through biochemical and behavioral examinations. The study found that Tartrazine exposure at environmentally relevant concentrations induced oxidative stress, cellular damage, and neuro-biochemical changes. Gene expression analyses revealed disruptions in genes associated with antioxidant defense, mitochondrial dysfunction, inflammatory response, apoptosis activation, and neural development. The study also showed altered behavior depending on the concentration of Tartrazine exposure. These findings highlight the neurodevelopmental effects of Tartrazine at environmentally relevant concentrations and provide valuable insights into the molecular mechanisms involved, particularly mitochondria-mediated apoptosis.
JOURNAL OF HAZARDOUS MATERIALS
(2024)