Review
Cell Biology
Vikash Kumar Yadav, Corentin Claeys Bouuaert
Summary: The article outlines the latest advances in understanding the mechanism of meiotic DSB formation, emphasizing the tight regulation of DSBs and the significance of recombination mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Taicong Tan, Yingjin Tan, Ying Wang, Xiao Yang, Binyuan Zhai, Shuxian Zhang, Xuan Yang, Hui Nie, Jinmin Gao, Jun Zhou, Liangran Zhang, Shunxin Wang
Summary: This study reveals that negative supercoils play a crucial role in connecting interference and crossover interference in meiosis, and top2 gene mutations affect the accumulation and relief of negative supercoils, thereby regulating the strength of crossover interference.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Florencia Pratto, Kevin Brick, Gang Cheng, Kwan-Wood Gabriel Lam, Jeffrey M. Cloutier, Daisy Dahiya, Stephen R. Wellard, Philip W. Jordan, R. Daniel Camerini-Otero
Summary: This passage discusses the distribution of genetic recombination in the genome, the role of the PRDM9 protein, and the impact of meiotic replication on recombination. Research findings show that meiotic DNA replication is distinct from replication in somatic cells, and this has important implications for understanding genetic recombination and the recombination potential of individual chromosomes.
Article
Biochemistry & Molecular Biology
Xiaofan Jin, Geoff Fudenberg, Katherine S. Pollard
Summary: Active, spatially accessible genomic regions during meiotic prophase are associated with DSB-favored loci, which further adopt a transient locally active configuration in early prophase. Conversely, crossover formation is depleted among DSBs in spatially accessible regions during meiotic prophase, particularly within gene bodies. Active chromatin regions have smaller average loop sizes in mammalian meiosis, indicating differences in chromatin architecture along chromosomal axes are associated with variable recombination activity.
Editorial Material
Biochemistry & Molecular Biology
Ilona Faustova, Mart Loog
Summary: Study by Hossain et al. (2021) reveals that ORC1 and CDC6 interact during pre-replicative complex formation in G1, mediated by SLiMs in IDRs and regulated by CDKs.
Article
Cell Biology
Xuan Yang, Binyuan Zhai, Shunxin Wang, Xiangfei Kong, Yingjin Tan, Lin Liu, Xiao Yang, Taicong Tan, Shuxian Zhang, Liangran Zhang
Summary: RNA-DNA hybrids formed at ssDNA ends of DSBs actively regulate meiotic recombination in budding yeast, affecting the efficiency of sporulation and spore viability. The accumulation of RNA-DNA hybrids competes with Rad51/Dmc1, impairs homolog bias, and decreases crossover and noncrossover recombination.
Review
Biochemistry & Molecular Biology
Kris G. Alavattam, So Maezawa, Paul R. Andreassen, Satoshi H. Namekawa
Summary: In this article, the authors reviewed the mechanisms underlying the initiation of two essential processes in mammalian male meiosis: meiotic sex chromosome inactivation (MSCI) and XY-body formation. They proposed the hypothesis that phase separation plays a role in the initiation of MSCI and the formation of the XY body.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jonna Heldrich, Carolyn R. Milano, Tovah E. Markowitz, Sarah N. Ur, Luis A. Vale-Silva, Kevin D. Corbett, Andreas Hochwagen
Summary: Successful meiotic recombination requires conserved axis proteins, which distribute on chromosomes through two independent pathways: a cohesin-dependent pathway and a cohesin-independent pathway. These pathways result in a stereotypic distribution pattern and the formation of chromatin islands that influence chromosome structure.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Matthew Charman, Nicholas Grams, Namrata Kumar, Edwin Halko, Joseph M. Dybas, Amber Abbott, Krystal K. Lum, Daniel Blumenthal, Elene Tsopurashvili, Matthew D. Weitzman
Summary: Biomolecular condensates formed by phase separation can compartmentalize and regulate cellular processes. Evidence suggests that membraneless subcellular compartments in virus-infected cells also form by phase separation. Here we demonstrate that phase separation of the human adenovirus 52-kDa protein plays a critical role in the coordinated assembly of infectious progeny particles. Our findings identify essential requirements for coordinated assembly of progeny particles and demonstrate that phase separation of a viral protein is critical for production of infectious progeny during adenovirus infection.
Article
Genetics & Heredity
Stanley Dean Rider Jr, French J. J. Damewood, Rujuta Yashodhan Gadgil, David C. C. Hitch, Venicia Alhawach, Resha Shrestha, Matilyn Shanahan, Nathen Zavada, Michael Leffak
Summary: Unbiased genetic screens using a lentiviral shRNA library identified genes that suppress break-induced mutagenesis in human cells with fragile non-B DNA. These genes have roles in DNA replication and repair, chromatin modification, responses to ionizing radiation, and replication fork-associated proteins. Novel loci implicated in BIR were also identified. Knockdown of selected candidates increased the frequency of nucleoside analog resistance and DNA rearrangements near the non-B DNA.
Article
Biology
Wei Xu, Chao Liu, Zhe Zhang, Changbin Sun, Qin Li, Kuan Li, Hui Jiang, Wei Li, Qianwen Sun
Summary: DNA End tailing and sequencing (DEtail-seq) is a novel technique that efficiently and accurately maps and studies the 3' ends of meiotic double-strand breaks (DSBs) in different species. The study found that the 3' ends of DSBs in budding yeast are stable and undergo minimal resection. In the mouse genome, DSBs at the 3' ends are strongly enriched in de novo H3K4me3 peaks during the leptotene stage. Meiotic DSB hotspots in humans are found to be enriched near common fragile sites, particularly at CCCTC-binding factor (CTCF)-associated enhancers. Therefore, DEtail-seq provides a powerful method for detecting DSB ends in various species and the findings offer new insights into the distribution and regulation of meiotic DSB hotspots.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Cell Biology
Wei He, Gerrik F. Verhees, Nikhil Bhagwat, Ye Yang, Dhananjaya S. Kulkarni, Zane Lombardo, Sudipta Lahiri, Pritha Roy, Jiaming Zhuo, Brian Dang, Andriana Snyder, Shashank Shastry, Michael Moezpoor, Lilly Alocozy, Kathy Gyehyun Lee, Daniel Painter, Ishita Mukerji, Neil Hunter
Summary: The study reveals the importance of SUMO protein modification in crossover control of Msh4, facilitating the formation of MutSg complex and ensuring at least one crossover for each pair of chromosomes. SUMOylation of Msh4 not only enhances the assembly of MutSg before JM formation, but may also play a role in downstream functions.
DEVELOPMENTAL CELL
(2021)
Article
Microbiology
Hui Wang, Nicole Beier, Christian Boedeker, Helena Sztajer, Petra Henke, Meina Neumann-Schaal, Johannes Mansky, Manfred Rohde, Joerg Overmann, Joern Petersen, Frank Klawonn, Martin Kucklick, Susanne Engelmann, Juergen Tomasch, Irene Wagner-Doebler
Summary: Outer membrane vesicles (OMVs) secreted by Dinoroseobacter shibae during cell division contain DNA enriched for specific gene regions, potentially repaired from overreplication. The vesicle proteome includes proteins interacting with peptidoglycan during division. The FtsK-dif-XerC/XerD molecular machinery may provide a novel route for DNA incorporation into OMVs.
Article
Cell Biology
Dima Daccache, Emma De Jonge, Pascaline Liloku, Karen Mechleb, Marita Haddad, Sam Corthaut, Yann G. -J. Sterckx, Alexander N. N. Volkov, Corentin Claeys Bouuaert
Summary: In this study, the authors describe the structural and functional properties of the meiotic DNA double-strand break complex. They show that the Rec114-Mei4 and Mer2 proteins are important for complex condensation and that their architectures are evolutionarily conserved. The study presents models of the Rec114-Mei4 and Mer2 complexes supported by various techniques, and reveals their DNA-binding properties.
GENES & DEVELOPMENT
(2023)
Article
Multidisciplinary Sciences
Toshihisa Yashiro, Yi-Kai Tea, Cara Van der Wal, Tomonari Nozaki, Nobuaki Mizumoto, Simon Hellemans, Kenji Matsuura, Nathan Lo
Summary: The study found that in some animals, males play important roles beyond providing gametes, which may prevent the evolution of obligate asexuality. Male termites promote genetic heterozygosity within populations through sex-linked chromosomes, while some asexual termite populations, generated through intraspecific hybridization, exhibit higher heterozygosity compared to sexual populations and have replaced them in some areas.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Genetics & Heredity
Matthew Robert Paul, Tovah Elise Markowitz, Andreas Hochwagen, Sevinc Ercan
Article
Biology
David V. Phizicky, Luke E. Berchowitz, Stephen Pbell
Article
Genetics & Heredity
Matthew Robert Paul, Tovah Elise Markowitz, Andreas Hochwagen, Sevinc Ercan
Article
Biotechnology & Applied Microbiology
Luis A. Vale-Silva, Tovah E. Markowitz, Andreas Hochwagen
Article
Biochemistry & Molecular Biology
David V. Phizicky, Stephen P. Bell
Review
Genetics & Heredity
Matthew Robert Paul, Andreas Hochwagen, Sevinc Ercan
Article
Biochemistry & Molecular Biology
Andres Mansisidor, Temistocles Molinar, Priyanka Srivastava, Demetri D. Dartis, Adriana Pino Delgado, Hannah G. Blitzblau, Hannah Klein, Andreas Hochwagen
Article
Multidisciplinary Sciences
Ramon Y. Rios-Morales, Sze Ham Chan, Stephen P. Bell
Article
Multidisciplinary Sciences
Vijayalakshmi V. Subramanian, Xuan Zhu, Tovah E. Markowitz, Luis A. Vale-Silva, Pedro A. San-Segundo, Nancy M. Hollingsworth, Scott Keeney, Andreas Hochwagen
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Pablo De Ioannes, Victor A. Leon, Zheng Kuang, Miao Wang, Jef D. Boeke, Andreas Hochwagen, Karim-Jean Armache
NATURE COMMUNICATIONS
(2019)
Article
Biology
Kanokwan Champasan, Caitlin Blank, Larry J. Friedman, Jeff Gelles, Stephen P. Bell
Article
Biology
Lorraine De Jesus-Kim, Larry J. Friedman, Marko Looke, Christian K. Ramsoomair, Jeff Gelles, Stephen P. Bell
Summary: The committed step of eukaryotic DNA replication occurs when Mcm2-7 replicative helicases are activated. Helicase activation requires the recruitment of Cdc45 and GINS to form CMGs. The study found that Cdc45 and GINS are recruited to Mcm2-7 in two stages, with phosphorylation modulating the process.
Article
Biochemistry & Molecular Biology
Alexandra M. Pike, Caitlin M. Friend, Stephen P. Bell
Summary: Replication protein A (RPA) plays critical roles in eukaryotic DNA replication, DNA damage response, and DNA repair by binding to single-stranded DNA (ssDNA). We found that Escherichia coli SSB can fully substitute for RPA in promoting origin DNA unwinding, while T4 bacteriophage Gp32 cannot. Our study reveals the requirement for specific modes of ssDNA binding in extensive origin DNA unwinding and identifies different RPA domains that impact replication fork function.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Audra L. Amasino, Shalini Gupta, Larry J. Friedman, Jeff Gelles, Stephen P. Bell
Summary: Eukaryotic DNA replication occurs only once per cell cycle, which is regulated by the temporal separation of helicase loading and activation. Phosphorylation of ORC by CDK inactivates helicase loading by preventing the recruitment of a second Mcm2-7 helicase and destabilizing the first Mcm2-7 ring closure. This phosphorylation also inhibits the formation of the MO complex, which is necessary for stable closure of the first Mcm2-7. These findings demonstrate that ORC phosphorylation affects multiple steps of helicase loading and elucidate the two-step process of the first Mcm2-7 ring closure.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Annie Zhang, Larry J. Friedman, Jeff Gelles, Stephen P. Bell
Summary: This study reveals the importance of dynamic protein-DNA interactions in ensuring bidirectional DNA replication during origin licensing. The results show that ORC can ensure head-to-head helicase alignment by sequentially loading Mcm2-7, and that controlled DNA sliding allows ORC to access secondary DNA-binding sites at different locations relative to the initial binding site.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)