Article
Chemistry, Multidisciplinary
Lei Qiao, Min Gao, Xiaoqing Yi, Hui Peng, Ruijie Zhang, Wanqing Yao, Gengyun Sun, Xiaoyan He
Summary: This study developed a cell membrane biomimetic core-shell system for light-controllable, precise gene editing, and successfully applied it in inhibiting tumor metastasis and enhancing chemotherapy effects. The system consisted of a core for CRISPR-Cas9/sgRNA plasmid loading and a shell camouflaged by a cell membrane, which induced lysosomal escape and payload release through reactive oxygen species production. By using this system, the expression of VEGF and Vimentin in H1299 tumor cells decreased, leading to enhanced antimetastatic effects. The results demonstrate that the membrane-camouflaged system combined with light augmentation provides a potential solution for in vivo delivery of CRISPR-Cas9 and cancer therapy.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Biomedical
Sarah Y. Neshat, Chun Hei Ryan Chan, Jawaun Harris, Osamah M. Zmily, Savannah Est-Witte, Johan Karlsson, Sydney R. Shannon, Manav Jain, Joshua C. Doloff, Jordan J. Green, Stephany Y. Tzeng
Summary: Immuno-oncology therapies aim to induce sustained tumor regression, but current methods are in need of improvement. An antigen-free method of cancer immunotherapy that stimulates the immune system without knowledge of neoantigens has been developed, along with a local delivery system to minimize systemic toxicity. A gene delivery nanoparticle platform has been engineered to reprogram the tumor microenvironment and activate cytotoxic lymphocytes, resulting in tumor regression and clearance.
Review
Chemistry, Multidisciplinary
Chavee Laomeephol, Sudartip Areecheewakul, Supannikar Tawinwung, Koramit Suppipat, Preedakorn Chunhacha, Nuno M. Neves, Jittima Amie Luckanagul
Summary: Chimeric antigen receptor (CAR) T cell therapy has shown remarkable efficacy in cancer treatment, but its complex manufacturing process limits its application. In vivo generation of CAR T cells provides an alternative immunotherapy. Hyaluronic acid (HA) is a natural biopolymer that can be used for gene delivery due to its inherent properties. This review discusses the potential of HA in delivering CAR constructs and provides insights into rational design of HA-based systems for efficient CAR delivery to circulating T cells.
Article
Chemistry, Multidisciplinary
Yu Seon Kim, Antonios G. Mikos
Summary: The review discusses several bottom-up strategies that have emerged in engineering MSC behavior for bone and cartilage tissue engineering, including gene delivery, gene editing, and subpopulation isolation. In contrast to a top-down method, the bottom-up method involves direct modulation of stem cell behavior without relying on environmental cues.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Multidisciplinary Sciences
Nicole Prodan, Faheem Ershad, Arfaxad Reyes-Alcaraz, Luge Li, Brandon Mistretta, Lei Gonzalez, Zhoulyu Rao, Cunjiang Yu, Preethi H. Gunaratne, Na Li, Robert J. Schwartz, Bradley K. McConnell
Summary: This study presents an effective protocol for directly reprogramming human cardiomyocytes into cardiac Purkinje-like cells using small molecules. The generated cells exhibit genetic and functional similarities to native cardiac Purkinje cells, showing expression of key cardiac Purkinje genes and increased velocity of electrical signal conduction. This research has significant implications for the development of optimized cell therapies for heart regeneration.
Article
Multidisciplinary Sciences
In-Kyu Lee, Allison L. Ludwig, M. Joseph Phillips, Juhwan Lee, Ruosen Xie, Benjamin S. Sajdak, Lindsey D. Jager, Shaoqin Gong, David M. Gamm, Zhenqiang Ma
Summary: Polymeric scaffolds are being used in therapeutics for blinding disorders affecting the outer retina, with recent advances in engineering producing optimized structures for retinal pigment epithelium delivery. A new biodegradable scaffold patterned for high-density photoreceptor replacement has been developed, which may have applications in multicellular layers for outer retinal reconstruction and other tissues. This approach could also be useful for producing various micro- and nanoscale structures for multilayered cell delivery.
Review
Chemistry, Multidisciplinary
Yujung Chang, Sungwoo Lee, Jieun Kim, Chunggoo Kim, Hyun Soo Shim, Seung Eun Lee, Hyeok Ju Park, Jeongwon Kim, Soohyun Lee, Yong Kyu Lee, Sungho Park, Junsang Yoo
Summary: Gene therapy is a promising approach in regenerative medicine that involves transferring genetic material to treat diseases. Specifically, gene therapy for neurological disorders has made significant progress, with adeno-associated viruses being used for targeted delivery of therapeutic genes. Direct lineage reprogramming (DLR) has emerged as a potential alternative to conventional stem cell therapy for incurable diseases, but its clinical application is hindered by low efficiency. Researchers have explored innovative strategies such as using nanoporous particles to improve the reprogramming efficiency of DLR-induced neurons. Discussing these approaches can further advancements in gene therapies for neurological disorders.
Article
Biochemical Research Methods
Jia Ping Tan, Xiaodong Liu, Jose M. Polo
Summary: This article describes a method for reprogramming human fibroblasts into induced trophoblast stem cells, along with steps for their molecular and functional characterization.
Review
Chemistry, Multidisciplinary
Shiva Soltani Dehnavi, Zahra Eivazi Zadeh, Alan R. Harvey, Nicolas H. Voelcker, Clare L. Parish, Richard J. Williams, Roey Elnathan, David R. Nisbet
Summary: The incorporation of nanotechnology in regenerative medicine, specifically in cell reprogramming, holds great potential and importance. Nanomaterials as delivery tools can overcome the limitations of traditional approaches and improve efficiency.
ADVANCED MATERIALS
(2022)
Article
Cell Biology
Marcel Rose, Katrin Domsch, Jakob Bartle-Schultheis, Ingolf Reim, Christoph Schaub
Summary: The genesis of syncytial muscles is typically considered an irreversible developmental process, and the mesodermal master regulator Twist plays a key role in the reprogramming process by regulating the activity of the Hippo pathway effector Yorkie. This study provides evidence that Twist is required for the initiation of syncytial muscle dedifferentiation and fragmentation, and fibroblast growth factor receptor (FGFR) Ras-mitogen-activated protein kinase (MAPK) signaling maintains Twist expression in resulting mononucleated myoblasts.
Article
Multidisciplinary Sciences
Thomas Kisby, Irene de Lazaro, Maria Stylianou, Giulio Cossu, Kostas Kostarelos
Summary: The study demonstrated that adenoviral delivery of OSKM can induce partial reprogramming of postnatal cardiomyocytes, with enhanced cell cycle activation, but the reprogramming is transient and cardiomyocyte-specific characteristics are spontaneously recovered by day 15 after viral transduction.
Article
Pharmacology & Pharmacy
Leyla A. Ovchinnikova, Stanislav S. Terekhov, Rustam H. Ziganshin, Dmitriy Bagrov, Ioanna N. Filimonova, Arthur O. Zalevsky, Yakov A. Lomakin
Summary: Engineered EVs produced by human cell line have shown potential in therapeutic delivery by efficiently loading active cargo into target cells. The use of EVs as carriers for protein therapeutics holds promise in enhancing drug efficiency.
Article
Cell Biology
Jennyfer Levoux, Alexandre Prola, Peggy Lafuste, Marianne Gervais, Nathalie Chevallier, Zeynab Koumaiha, Kaouthar Kefi, Laura Braud, Alain Schmitt, Azzedine Yacia, Aurelie Schirmann, Barbara Hersant, Mounia Sid-Ahmed, Sabrina Ben Larbi, Katerina Komrskova, Jakub Rohlena, Frederic Relaix, Jiri Neuzil, Anne-Marie Rodriguez
Summary: Platelets enhance the therapeutic potential of MSCs by transferring mitochondria and promoting pro-angiogenic activity. Activation of the de novo fatty acid synthesis pathway is required for increased secretion of pro-angiogenic factors by platelet-preconditioned MSCs. Testing platelet mitochondria quality before clinical use is crucial for optimizing their effects.
Article
Multidisciplinary Sciences
Tiep Tien Nguyen, Duc-Vinh Pham, Junhyeung Park, Cao Dai Phung, Mahesh Raj Nepal, Mahesh Pandit, Manju Shrestha, Youlim Son, Mili Joshi, Tae Cheon Jeong, Pil-Hoon Park, Dong-Young Choi, Jae-Hoon Chang, Ju-Hyun Kim, Jae-Ryong Kim, Il-Kug Kim, Chul Soon Yong, Jong Oh Kim, Jong-Hyuk Sung, Hu-Lin Jiang, Hyung-Sik Kim, Simmyung Yook, Jee-Heon Jeong
Summary: Researchers engineered hybrid spheroids of mesenchymal stem cells with rapamycin-releasing microparticles to prevent immune rejection of islet xenografts. The hybrid spheroids significantly prolonged islet survival times and promoted the generation of regulatory T cells, making them a promising platform for the efficient use of MSCs in transplantation.
Article
Cell & Tissue Engineering
Maria Questa, Maryam Moshref, Robert J. Jimenez, Veronica Lopez-Cervantes, Charles K. Crawford, Matthew L. Settles, Pablo J. Ross, Amir Kol
Summary: By conducting ATAC-seq analysis on canine stromal and pluripotent stem cells, we discovered that the barriers preventing reprogramming of adult stromal cells to iPSC may be attributed to differences in chromatin accessibility landscape, providing a theoretical basis for targeted improvement of canine somatic cell reprogramming in the future.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Editorial Material
Oncology
Eva Tuduri, Ruben Nogueiras
Editorial Material
Endocrinology & Metabolism
Eva Tuduri, Ruben Nogueiras
Review
Endocrinology & Metabolism
Angel Nadal, Ivan Quesada, Eva Tuduri, Ruben Nogueiras, Paloma Alonso-Magdalena
NATURE REVIEWS ENDOCRINOLOGY
(2017)
Article
Physiology
Juan Martinez-Pinna, Sergi Soriano, Eva Tuduri, Angel Nadal, Fernando de Castro
FRONTIERS IN PHYSIOLOGY
(2018)
Review
Endocrinology & Metabolism
Laura Marroqui, Eva Tuduri, Paloma Alonso-Magdalena, Ivan Quesada, Angel Nadal, Reinaldo Sousa dos Santos
JOURNAL OF ENDOCRINOLOGY
(2018)
Review
Endocrinology & Metabolism
Eva Tuduri, Laura Marroqui, Reinaldo S. Dos Santos, Ivan Quesada, Esther Fuentes, Paloma Alonso-Magdalena
FRONTIERS IN ENDOCRINOLOGY
(2018)
Article
Multidisciplinary Sciences
Eva Tuduri, Maria M. Glavas, Ali Asadi, Robert K. Baker, Cara E. Ellis, Galina Soukhatcheva, Marjolaine Philit, Frank K. Huynh, James D. Johnson, C. Bruce Verchere, Timothy J. Kieffer
SCIENTIFIC REPORTS
(2019)
Article
Endocrinology & Metabolism
Cristina Quesada-Candela, Eva Tuduri, Laura Marroqui, Paloma Alonso-Magdalena, Ivan Quesada, Angel Nadal
METABOLISM-CLINICAL AND EXPERIMENTAL
(2020)
Article
Endocrinology & Metabolism
Myriam P. Hoyeck, Rayanna C. Merhi, Hannah L. Blair, C. Duncan Spencer, Mikayla A. Payant, Diana I. Martin Alfonso, Melody Zhang, Geronimo Matteo, Melissa J. Chee, Jennifer E. Bruin
MOLECULAR METABOLISM
(2020)
Article
Endocrinology & Metabolism
Geronimo Matteo, Myriam P. Hoyeck, Hannah L. Blair, Julia Zebarth, Kayleigh R. C. Rick, Andrew Williams, Remi Gagne, Julie K. Buick, Carole L. Yauk, Jennifer E. Bruin
Summary: The study found that prolonged exposure to low-dose TCDD had minimal effects on glucose homeostasis and islet morphology in chow-fed male and female mice, but promoted maladaptive metabolic responses in HFD-fed females.
Article
Endocrinology & Metabolism
Diana Grajales, Patricia Vazquez, Monica Ruiz-Rosario, Eva Tuduri, Mercedes Mirasierra, Vitor Ferreira, Ana B. Hitos, Dora Koller, Pablo Zubiaur, Juan C. Cigudosa, Francisco Abad-Santos, Mario Vallejo, Ivan Quesada, Boaz Tirosh, Gil Leibowitz, Angela M. Valverde
Summary: Both olanzapine and aripiprazole induced adverse effects on beta cell function in female mice, leading to weight gain, glucose intolerance, and impaired insulin secretion. Aripiprazole had a stronger impact on metabolic alterations, likely due to its modulation of the serotonergic system.
Article
Geriatrics & Gerontology
Eva Tuduri, Sergi Soriano, Lucia Almagro, Anabel Garcia-Heredia, Alex Rafacho, Paloma Alonso-Magdalena, Angel Nadal, Ivan Quesada
Summary: Aging is associated with decreased beta-cell adaptations and insulin sensitivity. Aged mice with low insulin sensitivity demonstrate worsened glucose metabolism and impaired insulin secretion, possibly due to compromised mitochondrial function.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2022)
Article
Medicine, Research & Experimental
Tao Liang, Tairan Qin, Fei Kang, Youhou Kang, Li Xie, Dan Zhu, Subhankar Dolai, Dafna Greitzer-Antes, Robert K. Baker, Daorong Feng, Eva Tuduri, Claes-Goran Ostenson, Timothy J. Kieffer, Kate Banks, Jeffrey E. Pessin, Herbert Y. Gaisano
Article
Multidisciplinary Sciences
Maria M. Glavas, Queenie Hui, Eva Tuduri, Suheda Erener, Naomi L. Kasteel, James D. Johnson, Timothy J. Kieffer
SCIENTIFIC REPORTS
(2019)
Article
Endocrinology & Metabolism
Nelly Saber, Jennifer E. Bruin, Shannon O'Dwyer, Hellen Schuster, Alireza Rezania, Timothy J. Kieffer