Article
Chemistry, Multidisciplinary
Kaiqing Ma, He Yang, Tianruo Shen, Yongkang Yue, Lingling Zhao, Xiaogang Liu, Fangjun Huo, Caixia Yin
Summary: Researchers developed a new probe CM-Mit for accurate detection of thiols in mitochondria. The probe has fast response and protein labeling ability, preventing diffusion out of the mitochondria, and can be used for drug screening and studying ferroptosis.
Review
Cell Biology
Jie Cheng, Casey J. Keuthan, Noriko Esumi
Summary: SIRT6 is a versatile protein that plays a key role in maintaining cellular and organismal homeostasis. It has various functions in gene silencing, metabolism, DNA repair, antioxidant defense, inflammation, aging and longevity, early development, stress response, macrophage polarization, and mitochondrial homeostasis. Recent studies have revealed its critical functions in the retina and RPE, such as regulating energy metabolism, protecting against oxidative stress, and participating in early neurodegenerative events in diabetic retinopathy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
John A. Collins, Maryna Kapustina, Jesalyn A. Bolduc, James F. W. Pike, Brian O. Diekman, Kimberlee Mix, Susan Chubinskaya, Emrah Eroglu, Thomas Michel, Leslie B. Poole, Cristina M. Furdui, Richard F. Loeser
Summary: SIRT6, a nuclear localized deacetylase protein, is a critical regulator of aging processes. It can promote resistance to oxidative stress and influences specific members of the Prx catalytic cycle in chondrocytes to maintain redox homeostasis. Age and oxidative stress conditions alter SIRT6 activity, suggesting potential therapeutic targets for maintaining redox balance in joint tissues and preventing osteoarthritis progression.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Plant Sciences
Lin Li, Hao Zhang, Beibei Chen, Bingke Xia, Ruyuan Zhu, Yage Liu, Xuan Dai, Zimengwei Ye, Dandan Zhao, Fangfang Mo, Sihua Gao, Alexander N. Orekhov, Marc Prentki, Lili Wang, Shuzhen Guo, Dongwei Zhang
Summary: The study found that BZBS can improve cognitive and motor functions in aging mice, maintain redox balance, alleviate immunosenescence, and preserve telomerase activity and length. Additionally, BZBS can inhibit rapid aging in mice by reducing the expressions of P53, caspase-3, and Bax, while increasing the expressions of Sirt6, p-HO-1, p-NRF2, PGC-1 alpha, and Bcl-2.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Cell Biology
Shuya Gao, Qingchen Yang, Yue Peng, Weixian Kong, Zekun Liu, Zhe Li, Jiawen Chen, Mengmeng Bao, Xie Li, Yubin Zhang, Xiaohong Bian, Liang Jin, Hanwen Zhang, Yuexin Zhang, Daniel Sanchis, Fangrong Yan, Junmei Ye
Summary: This study highlights the beneficial role of SIRT6 in mitochondrial biogenesis and oxidative stress regulation in cardiomyocytes during obesity-induced cardiac injury, providing new insights into the pathogenesis of obesity-associated lipotoxicity in the heart.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Chemistry, Organic
Maria L. Alfieri, Alice Cariola, Lucia Panzella, Alessandra Napolitano, Marco d'Ischia, Luca Valgimigli, Orlando Crescenzi
Summary: This study provides evidence for the addition mechanism of thiol compounds to quinones and discovers an anomalous 1,6-type regiochemistry compared to the usual 1,4-nucleophilic addition. These findings would prompt a revision of the commonly accepted mechanisms for thiol-quinone conjugation and stimulate further research in this field.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Arjan de Groot, Laurence Blanchard, Nicolas Rouhier, Pascal Rey
Summary: Deinococcus species have remarkable tolerance to oxidative damage in extreme environmental conditions. Thiol reductases (TRs) play important roles in metabolism regulation and stress responses. The study reveals that Deinococcus possesses multiple enzyme systems that protect proteins and maintain redox status. Recent research further confirms the importance of TRs in Deinococcus' tolerance to oxidative treatments.
Article
Biochemistry & Molecular Biology
Hayato Irokawa, Satoshi Numasaki, Shin Kato, Kenta Iwai, Atsushi Inose-Maruyama, Takumi Ohdate, Gi-Wook Hwang Section, Takashi Toyama, Toshihiko Watanabe, Shusuke Kuge
Summary: Redox regulation of proteins via cysteine residue oxidation plays a critical role in controlling cellular signaling pathways. PKM2 is essential for metabolic shift in cancer cells under oxidative stress. Oxidation of Cys358 and Cys424 may contribute to eliminating excessive ROS and oxidative stress.
BIOCHEMICAL JOURNAL
(2021)
Article
Chemistry, Applied
Asako Narai-Kanayama, Kazuhiro Chiku, Hiroshi Ono, Takamitsu Momoi, Miho Hiwatashi-Kanno, Akiko Kobayashi, Hiroko Matsuda, Mitsuru Yoshida, Tsutomu Nakayama
Summary: Theaflavin, the polyphenol responsible for the orange color and bioactivities of black tea, can undergo autoxidation to change its color to brown under neutral and mild alkaline conditions. The presence of thiol compounds such as cysteine, glutathione, or N-acetyl cysteine can inhibit the browning of theaflavin solution and lead to a decrease in the concentrations of both theaflavin and thiol group over time. LC-MS analysis revealed the formation of novel adducts of thiol compounds. The chemical structures of theaflavin-cysteine and theaflavin-glutathione adducts were determined using NMR, showing the addition of a new cyclic structure to the theaflavin-cysteine adduct.
Article
Chemistry, Multidisciplinary
Chuan Wan, Zhanfeng Hou, Dongyan Yang, Ziyuan Zhou, Hongkun Xu, Yuena Wang, Chuan Dai, Mingchan Liang, Jun Meng, Jiean Chen, Feng Yin, Rui Wang, Zigang Li
Summary: Visible-light-mediated methods for cysteine-selective bioconjugation suffer from bio-incompatible conditions and slow kinetics. Here, we report a transition metal-free thiol-sulfoxonium ylide photo-click reaction that enables bioconjugation under bio-compatible conditions. It demonstrates high cysteine-selectivity and rapid reaction kinetics using riboflavin derivatives as photocatalysts. This method provides efficient tools for exploring the druggable content of the human proteome.
Article
Biochemistry & Molecular Biology
Moua Yang, Robert Flaumenhaft
Summary: Oxidative stress is a characteristic of many systemic diseases associated with thrombosis, and thiol isomerases play a key role in protein folding. Recent studies show that the oxidoreductase activity of thiol isomerases in thrombus formation is controlled by the redox environment and may potentially control thrombotic potential by modifying substrate networks.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Biochemistry & Molecular Biology
Feng-Fan Chiang, Te-Hsin Chao, Shih-Chien Huang, Chien-Hsiang Cheng, Yu-Yao Tseng, Yi-Chia Huang
Summary: Cysteine is associated with oxidative stress and GSH-related antioxidant capacity in colorectal cancer patients. After tumor resection, cysteine levels increase in plasma and tissues, along with increased oxidative stress indicators and antioxidant enzyme activities. Cysteine levels in tumor and adjacent normal tissues are associated with homocysteine levels, indicating their involvement in oxidative stress and antioxidant capacities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Peter W. Stacpoole, Charles E. McCall
Summary: Pyruvate dehydrogenase complexes (PDC) are key components of energy metabolism in all organisms. In eukaryotes, they play a crucial role in connecting glycolysis in the cytoplasm with the tricarboxylic acid cycle in the mitochondria. PDCs also impact the metabolism of amino acids and lipids, as well as oxidative phosphorylation. Its activity is essential for the metabolic and bioenergetic flexibility of organisms in response to developmental changes, nutrient availability, and various stresses.
Article
Biotechnology & Applied Microbiology
Nicolas Barraud, Sylvie Letoffe, Christophe Beloin, Joelle Vinh, Giovanni Chiappetta, Jean-Marc Ghigo
Summary: The study compared cysteine S-nitrosylation in aerobic and anaerobic biofilm and planktonic Escherichia coli cultures, identifying proteins with biofilm-specific S-nitrosylation status. Impairing S-nitrosylation in proteins related to redox homeostasis and amino acid synthesis altered important biofilm properties, such as motility, biofilm maturation, and resistance to oxidative stress, indicating the physiological basis of E. coli adaptation to the biofilm environment.
NPJ BIOFILMS AND MICROBIOMES
(2021)
Review
Endocrinology & Metabolism
Ying Wang, Tongtong Liu, Yuzi Cai, Weijing Liu, Jing Guo
Summary: This article discusses the important role of SIRT6 in glucose and lipid metabolism and its potential as a therapeutic target to improve glucose and lipid metabolism and alleviate DN occurrence and progression.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Neurosciences
Wenzhong Yang, Xueyan Zhou, Xin Wang, Jingyun Lee, Dan Wu, Peiqing Sun, Cristina M. Furdui, Tao Ma
Summary: AMP-activated protein kinase (AMPK) plays a crucial role in maintaining cellular energy balance and dysregulation of its signaling is linked to neuronal diseases. This study identified proteins sensitive to suppression of AMPK alpha isoforms, potentially informing future research on therapeutic targets for neurological disorders.
Article
Biochemistry & Molecular Biology
Kuanlin Wu, Hanzhi Wu, Wanqing Lyu, Youngjoo Kim, Cristina M. Furdui, Karen S. Anderson, Anthony J. Koleske
Summary: Abl family kinases are nonreceptor tyrosine kinases that are activated by phosphorylation upon interaction with PDGFR beta. Specific tyrosine residues play a crucial role in this phosphorylation process, leading to enhanced kinase activity and activation of Abl family kinases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Oncology
Christina M. Snyder, Monica M. Rohde, Cale D. Fahrenholtz, Jessica Swanner, John Sloop, George L. Donati, Cristina M. Furdui, Ravi Singh
Summary: The study shows that silver nanoparticles can selectively kill mesenchymal triple-negative breast cancer cells by causing lipid and protein oxidation, without harming normal cells. This suggests that stratification of TNBC subtypes may improve outcomes for other therapeutics with similar mechanisms.
Article
Oncology
Kimberly M. Burcher, Andrew T. Faucheux, Jeffrey W. Lantz, Harper L. Wilson, Arianne Abreu, Kiarash Salafian, Manisha J. Patel, Alexander H. Song, Robin M. Petro, Thomas Lycan, Cristina M. Furdui, Umit Topaloglu, Ralph B. D'Agostino, Wei Zhang, Mercedes Porosnicu
Summary: The study found that DDR gene mutations are significantly increased in older patients, patients with primary tumors in the larynx, patients with advanced stage cancer at diagnosis, and patients previously treated with chemotherapy and/or radiotherapy in head and neck squamous cell cancer (HNSCC). Patients with primary tumors in the oropharynx are less likely to have DDR gene mutations. DDR gene mutations identified in blood samples were associated with worse survival outcomes. The study highlights the importance of DDR gene mutations in HNSCC and supports further research on PARP inhibitors in precision oncology treatments.
Article
Biochemistry & Molecular Biology
Tom E. Forshaw, Julie A. Reisz, Kimberly J. Nelson, Rajesh Gumpena, J. Reed Lawson, Thomas J. Jonsson, Hanzhi Wu, Jill E. Clodfelter, Lynnette C. Johnson, Cristina M. Furdui, W. Todd Lowther
Summary: Human peroxiredoxins (Prx) are involved in various cellular functions and diseases, and can be repaired by human sulfiredoxin (Srx), hydrogen sulfide (H2S), and glutathione (GSH) after being hyperoxidized. Studying engineered Prx2 and Prx3 dimers, it was found that loss of the active site interface can make variants more resistant to repair, and it was revealed for the first time potential cooperation between Prx isoforms and GSH and H2S in supporting Srx activity.
Article
Biochemistry & Molecular Biology
Nicole P. Kasica, Xueyan Zhou, Qian Yang, Xin Wang, Wenzhong Yang, Helena R. Zimmermann, Caroline E. Holland, Elizabeth Koscielniak, Hanzhi Wu, Anderson O. Cox, Jingyun Lee, Alexey G. Ryazanov, Cristina M. Furdui, Tao Ma
Summary: This study demonstrates the therapeutic potential of inhibiting eEF2K kinase in improving synaptic plasticity impairments and cognitive dysfunction in AD model mice.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Neurosciences
Xin Wang, Xueyan Zhou, Beth Uberseder, Jingyun Lee, Caitlin S. Latimer, Cristina M. Furdui, C. Dirk Keene, Thomas J. Montine, Thomas C. Register, Suzanne Craft, Carol A. Shively, Tao Ma
Summary: The study on NHP model demonstrated an increased activity of AMPK alpha 2 in AD-like neuropathology, unrelated to AMPK alpha 1; CSF Abeta(42) showed a negative correlation with cerebral amyloid plaque density; A beta plaque burden correlated with brain A beta measurement levels.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Toxicology
Monica M. Rohde, Christina M. Snyder, John Sloop, Shane R. Solst, George L. Donati, Douglas R. Spitz, Cristina M. Furdui, Ravi Singh
Summary: The study identified distinct responses of cells to AgNPs and Ag+. AgNPs induce cell death through lipid peroxidation leading to proteotoxicity and necrotic cell death, while Ag+ triggers oxidative stress and apoptotic cell death by increasing H2O2 levels. The mechanism by which AgNPs kill mammalian cells was found to be independent of the contribution of Ag+ released in extracellular media.
PARTICLE AND FIBRE TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaofei Chen, Zhe Li, Caigang Zhu, Kirtikar Shukla, Tom E. Forshaw, Hanzhi Wu, Stephen A. Vance, Megan Madonna, Mark W. Dewhirst, Allen W. Tsang, Leslie B. Poole, Nimmi Ramanujam, Cristina M. Furdui, Kiran Kumar Solingapuram Sai, Buddhika Liyana Pathirannahel, S. Bruce King
Summary: Redox metabolism plays a crucial role in cancer and other diseases. This study presents a new radiotracer for in vivo imaging of protein oxidation, which has the potential for diagnosing tumors.
Article
Multidisciplinary Sciences
Diego R. Coelho, Flavio R. Palma, Veronica Paviani, Chenxia He, Jeanne M. Danes, Yunping Huang, Juliana C. P. Calado, Peter C. Hart, Cristina M. Furdui, Leslie B. Poole, Matthew J. Schipma, Marcelo G. Bonini
Summary: Acetylation of SOD2 changes its biochemical function from an antioxidant to a demethylase, promoting stem cell gene expression and contributing to tumor progression and metastasis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Qiwei Hu, Rama D. Yammani, Heather Brown-Harding, David R. Soto-Pantoja, Leslie B. Poole, John C. Lukesh
Summary: Doxorubicin (DOX) is a highly effective anticancer agent, but its dose-dependent cardiotoxicity limits its long-term use. This study describes a novel H2O2-responsive DOX hybrid codrug that releases hydrogen sulfide (H2S) to maintain the antitumor effects of DOX while reducing cardiotoxic side effects.
Article
Biochemistry & Molecular Biology
David L. Long, Charles E. McCall, Leslie B. Poole
Summary: This study demonstrates that LPS induces reversible oxidation within PDCE2 in THP-1 cells and primary human monocytes. Glutathionylation is associated with wild type PDCE2 but not mutant protein lacking the lipoamide-linking lysine residues. The combination treatment of LPS and MitoCDNB has a synergistic effect on PDCE2 glutathionylation, ROS production, and cell death, while showing differential effects on cytokine production.
Article
Engineering, Electrical & Electronic
Yueqiang Yu, Zhongyong Zhao, Yu Chen, Hanzhi Wu, Chao Tang, Wenwen Gu
Summary: This study discusses the application of impulse frequency response analysis in detecting motor winding short circuit faults, verifies the effectiveness and advantages of the method through experiments, and compares it with traditional methods.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2022)
Article
Biochemical Research Methods
Reetta Holmila, Hanzhi Wu, Jingyun Lee, Allen W. Tsang, Ravi Singh, Cristina M. Furdui
Summary: This study reveals that silver nanoparticles induce changes in protein abundance and reversible oxidation in a time and cell-line-dependent manner, impacting critical cellular processes with mitochondria as a main target. The mitochondrial networks and morphology in Calu-1 and NCI-H358 lung cells are differentially affected by AgNPs toxicity.
MOLECULAR & CELLULAR PROTEOMICS
(2021)