Article
Microbiology
Judah Ssekitoleko, Lonzy Ojok, Ahmed Abd El Wahed, Joseph Erume, Ahmad Amanzada, ElSagad Eltayeb, Kamal H. Eltom, Julius Boniface Okuni
Summary: This review discusses the mechanisms utilized by MAP to overcome the host defense system to achieve virulence, including putative MAP virulence genes and their probable roles in comparison to other mycobacteria. It also highlights the importance of understanding MAP virulence mechanisms in controlling and preventing paratuberculosis.
Article
Agriculture, Dairy & Animal Science
Vincent Rochard, Thierry Cochard, Stephanie Crapart, Vincent Delafont, Jean-Louis Moyen, Yann Hechard, Franck Biet
Summary: The prevalence of Johne's disease (JD) caused by Mycobacterium avium ssp. paratuberculosis (Map) is high and poses significant economic losses in the dairy industry worldwide. Recent studies have shown that Map may hinder the eradication of bovine tuberculosis (bTB) due to frequent co-infection. This study investigates the co-occurrence of amoebae and mycobacteria in water troughs of cattle herds monitored for JD or bTB, and suggests that amoebae may play a role in the transmission of JD.
Article
Microbiology
Alexander Byrne, Nathalie Bissonnette, Severine Ollier, Kapil Tahlan
Summary: Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's Disease (JD) in ruminants and poses significant economic loss to the global dairy industry. This study investigates the prevalence and impact of mixed strain infection (MSI) and microevolution on JD by analyzing MAP isolates from high-shedding animals. The results show evidence of MSIs and microevolution in most animals, with variations in short sequence repeats (SSRs) affecting protein products and potentially influencing the infectivity of the pathogen. These findings provide insights into the dynamics and evolution of MAP in JD.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Alexander Byrne, Nathalie Bissonnette, Severine Ollier, Kapil Tahlan
Summary: This study reveals the presence of mixed strain infection and microevolution in high-shedding animals, which could have implications for the development of Johne's Disease in dairy cattle.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agriculture, Dairy & Animal Science
Chloe Matthews, Aaron M. Walsh, Stephen V. Gordon, Bryan Markey, Paul D. Cotter, Jim O' Mahony
Summary: This study investigated the changes in the faecal microbiome of cattle exposed to MAP compared to a control group. Significant differences in taxonomic diversity and composition were observed at 3 months post inoculation, including changes in the relative abundance of Methanobrevibacter and Bifidobacterium genera.
Article
Agriculture, Dairy & Animal Science
B. W. Kirkpatrick, M. E. Cooke, M. Frie, K. R. B. Sporer, B. Lett, S. J. Wells, M. Coussens
Summary: This study identified genetic variations associated with susceptibility to MAP infection in Holstein cattle through genome-wide association analysis. The results suggest that genomic selection against MAP infection is feasible in Holstein cattle.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Chris Pickrodt, Karsten Donat, Udo Moog, Heike Koehler
Summary: This study determined the presence of Mycobacterium avium subsp. paratuberculosis (MAP) in the barn environment of a paratuberculosis-infected vaccinated dairy goat herd. Environmental samples were collected and examined using culture and qPCR methods. High animal traffic areas and samples collected during the indoor season were more likely to yield positive results. Dust was found to be the most suitable material for detecting MAP DNA, while bedding was suitable for MAP culture. Environmental sampling was shown to be an effective way to detect MAP in a dairy goat herd.
Article
Veterinary Sciences
Floor Biemans, Jamie Tratalos, Sandie Arnoux, George Ramsbottom, Simon J. More, Pauline Ezanno
Summary: This study investigated the impact of observed herd characteristics on the spread of Mycobacterium avium subspecies paratuberculosis (Map) on a national scale in Ireland. The research found that the probability of introducing infected animals into a herd increases with a higher number of animals being traded and the number of herds from which animals are sourced. Herds that engage in both buying and selling a large number of animals pose the highest risk of infection to other herds.
VETERINARY RESEARCH
(2022)
Article
Immunology
Taylor L. T. Wherry, Rohana Dassanayake, Eduardo Casas, Shankumar Mooyottu, John P. Bannantine, Judith R. Stabel
Summary: Vitamin D-3 compounds, especially 1,25(OH)(2)D-3, modulate immune responses in dairy cattle infected with MAP, impacting the viability of bacteria within macrophages.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Taylor L. T. Wherry, Shankumar Mooyottu, Judith R. Stabel
Summary: The role of vitamin D-3 in modulating immune responses in cattle, particularly in different stages of infection with Mycobacterium avium subspecies paratuberculosis (MAP), has been investigated in this study. The results suggest that vitamin D-3 can modulate cytokine signaling in cattle at different stages of infection with MAP and may have implications on disease progression.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Veterinary Sciences
Amanda Kravitz, Kevin Pelzer, Nammalwar Sriranganathan
Summary: Paratuberculosis, or Johne's Disease, is a chronic enteritis mainly affecting ruminants caused by MAP, leading to economic losses and potential zoonotic risk. The lack of effective treatments and vaccines complicates disease control strategies, while host genetics play a role in resistance and susceptibility. Candidate genes identified in studies overlap with those found in CD and tuberculosis.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Vincent P. Richards, Annette Nigsch, Paulina Pavinski Bitar, Qi Sun, Tod Stuber, Kristina Ceres, Rebecca L. Smith, Suelee Robbe Austerman, Ynte Schukken, Yrjo T. Grohn, Michael J. Stanhope
Summary: This study conducted a prolonged longitudinal analysis of MAP isolates and dairy cow Johne's disease phenotypes from three commercial dairy farms, identifying several genes associated with the disease. The results provide insights for improving strategies to control Johne's disease.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
John P. Bannantine, Judith R. Stabel, Darrell O. Bayles, Franck Biet
Summary: This study demonstrates how genomic analysis can improve diagnostic tests. Detecting Mycobacterium avium subsp. paratuberculosis infection in livestock is challenging but essential for preventing Johne's disease transmission. The commonly used IS900 primer sets for nucleic acid-based diagnostic tests have design errors that can be easily corrected to enhance detection sensitivity. The newly designed JB5 primer set showed improved sensitivity, and the use of legacy primers should be discontinued in future studies.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Reproductive Biology
Jessica Lobo Albuquerque Caldeira, Ana Carolina Silva Faria, Edgar Andres Diaz-Miranda, Tiago Jaquel Zilch, Sanely Lourenco da Costa Caliman, Denise Silva Okano, Jose Domingues Guimara, Junnia Luisa Pena, Wagner Faria Barbosa, Abelardo Silva Junior, Yung-Fu Chang, Maria Aparecida Scatamburlo Moreira
Summary: The study found that MAP affects the motility and vigor of bovine sperm, with higher concentrations of MAP leading to lower sperm performance. MAP was found to remain viable in samples of higher concentrations after thawing, indicating the potential transmission of this pathogen through artificial insemination.
Article
Immunology
Sandeep K. K. K. Gupta, Tania Wilson, Paul H. H. Maclean, Bernd H. A. Rehm, Axel Heiser, Bryce M. M. Buddle, D. Neil Wedlock
Summary: This study discusses vaccines for the prevention of bovine paratuberculosis and compares the immune responses to different vaccines. It found that a soluble fusion protein vaccine can induce strong antigen-specific antibody responses and a balanced Th1/Th17-cell immune response, potentially providing protective immunity against bovine tuberculosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Veterinary Sciences
Noah D. Cohen, Nathan M. Slovis, Steeve Giguere, Samantha Baker, M. Keith Chaffin, Lawrence R. Bernstein
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2015)
Article
Microbiology
Adrian Rangel-Vega, Lawrence R. Bernstein, Edna Ayerim Mandujano-Tinoco, Silvia Julieta Garcia-Contreras, Rodolfo Garcia-Contreras
FRONTIERS IN MICROBIOLOGY
(2015)
Article
Veterinary Sciences
Caroline S. Monk, Raymond W. Sweeney, Lawrence R. Bernstein, Marie-Eve Fecteau
AMERICAN JOURNAL OF VETERINARY RESEARCH
(2016)
Article
Microbiology
T. E. Norman, M. Batista, S. D. Lawhon, S. Zhang, K. R. Kuskie, A. K. Swinford, L. R. Bernstein, N. D. Cohen
JOURNAL OF CLINICAL MICROBIOLOGY
(2014)
Article
Pharmacology & Pharmacy
F. Sampieri, A. L. Allen, J. Alcorn, C. R. Clark, F. A. Vannucci, N. Pusterla, S. M. Mapes, K. R. Ball, P. M. Dowling, J. Thompson, L. R. Bernstein, C. J. Gebhart, D. L. Hamilton
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
(2014)
Article
Pharmacology & Pharmacy
F. Sampieri, J. Alcorn, A. L. Allen, C. R. Clark, F. A. Vannucci, N. Pusterla, S. Mapes, K. R. Ball, P. M. Dowling, J. Thompson, L. R. Bernstein, C. J. Gebhart, D. L. Hamilton
JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS
(2014)
Article
Anesthesiology
Lawrence R. Bernstein
Article
Microbiology
Carolyn E. Arnold, Angela Bordin, Sara D. Lawhon, Melissa C. Libal, Lawrence R. Bernstein, Noah D. Cohen
VETERINARY MICROBIOLOGY
(2012)
Article
Immunology
Sarah Hijazi, Daniela Visaggio, Mattia Pirolo, Emanuela Frangipani, Lawrence Bernstein, Paolo Visca
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Article
Infectious Diseases
Lorenzo Giacani, Lawrence R. Bernstein, Austin M. Haynes, B. Charmie Godornes, Giulia Ciccarese, Francesco Drago, Aurora Parodi, Sefora Valdevit, Luca Anselmi, Carlo Francesco Tomasini, Arthur M. Baca
PLOS NEGLECTED TROPICAL DISEASES
(2019)
Article
Engineering, Biomedical
Stacy Cereceres, Ziyang Lan, Laura Bryan, Michael Whitely, Thomas Wilems, Hunter Greer, Ellen Ruth Alexander, Robert J. Taylor, Lawrence Bernstein, Noah Cohen, Canaan Whitfield-Cargile, Elizabeth Cosgriff-Hernandez
APL BIOENGINEERING
(2019)
Meeting Abstract
Veterinary Sciences
F. Sampieri, A. L. Allen, J. Alcorn, C. R. Clark, F. A. Vannucci, N. Pusterla, K. R. Ball, P. M. Dowling, J. Thompson, L. R. Bernstein, C. J. Gebhart, D. L. Hamilton
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2012)
Meeting Abstract
Veterinary Sciences
F. Sampieri, J. Alcorn, A. L. Allen, C. R. Clark, F. A. Vannucci, N. Pusterla, K. R. Ball, P. M. Dowling, J. Thompson, L. R. Bernstein, C. J. Gebhart, D. L. Hamilton
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2012)
Article
Oncology
Lawrence R. Bernstein, Jacobus J. M. van der Hoeven, Robbert O. Boer
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2011)
Article
Veterinary Sciences
L. Alibrandi, R. Tognetti, O. Domenech, M. Croce, M. Giuntoli, G. Grosso, T. Vezzosi
Summary: This study assessed the feasibility and diagnostic reliability of a new smartphone-based ECG device in dogs, and found no significant differences compared to a traditional ECG device. The results suggest that the smartphone-based device is clinically reliable for assessing heart rate and rhythm in dogs.
VETERINARY JOURNAL
(2024)
