Article
Immunology
Elia Riquelme, Sabina Omarova, Berengere Ize, David O'Callaghan
Summary: Bacteria use the Tat system to export folded proteins. The Tat system is essential for the pathogen Brucella spp. By bioinformatic screening, 28 B. suis proteins with putative Tat signal sequences were identified.
INFECTION AND IMMUNITY
(2023)
Article
Multidisciplinary Sciences
Elin Movert, Jaume Salgado Bolarin, Christine Valfridsson, Jorge Velarde, Steinar Skrede, Michael Nekludov, Ole Hyldegaard, Per Arnell, Mattias Svensson, Anna Norrby-Teglund, Kyu Hong Cho, Eran Elhaik, Michael R. Wessels, Lars Raberg, Fredric Carlsson
Summary: The severity of diseases caused by microbial pathogens can vary due to the unique combination of host and pathogen genomes in each infection. In a study on invasive Streptococcus pyogenes infection, researchers found that the outcome is regulated by the interaction between human STING genotype and bacterial NADase activity. The activity of NADase variants expressed by invasive strains suppresses the production of type I interferon mediated by STING.
NATURE COMMUNICATIONS
(2023)
Review
Multidisciplinary Sciences
Sujata Kumari, Prajna Tripathi
Summary: Pathogens exploit host resources to survive, with nucleotide metabolism playing a crucial role in infection pathogenesis. Understanding how pathogens acquire and utilize nucleotides within a host can provide valuable insights into host-pathogen interactions and aid in the development of novel therapeutic strategies.
Article
Biochemistry & Molecular Biology
Tjeerd Pols, Shubham Singh, Cecile Deelman-Driessen, Bauke F. Gaastra, Bert Poolman
Summary: The breakdown of arginine in cells generates metabolic energy in the form of ATP through a pathway consisting of specific enzymes and a transporter. Various factors such as physicochemical conditions, effector composition, and concentration can affect enzyme activity. The study provides insights into the biochemical properties of the enzymes and transporter, as well as their response to different conditions and effector concentrations.
Article
Multidisciplinary Sciences
Wanpeng Wang, Jingyu Xia, Zining Wang, Zongze Shao
Summary: In this study, a bacterial cell sensing system for polycyclic aromatic hydrocarbons (PAHs) was identified. This system plays a critical role in sensing and promoting the metabolism of PAHs, which can be scavenged by various hydrocarbon-degrading bacteria.
Article
Immunology
Xin Yan, Sen Hu, Yan Yang, Da Xu, Wenxing Liu, Ganwu Li, Wentong Cai, Zhigao Bu
Summary: Brucella, an intracellular pathogen, causes chronic infections in mammals. This study compared host responses to wild-type and Tat-mutant strains of Brucella melitensis M28. Proteomic analysis revealed increased production of immune response-related proteins in response to Tat mutant infection, indicating a role of these proteins in restricting Tat mutant survival within macrophages. Additionally, Tat mutants displayed higher sensitivity to nitrosative stress, and NO production was shown to contribute to limiting Tat mutant survival within macrophages.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Deborah A. Powers, Matthew Jenior, Glynis Kolling, Jason Papin
Summary: The toxins produced by Clostridioides difficile are crucial for its pathogenicity. Understanding their regulation through the environment and intracellular metabolism can help attenuate the virulence of this bacterium. This study found that uptake of arginine and ornithine is particularly active in low toxin states and is dependent on intracellular fatty acid and large polymer metabolite pools.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Environmental Sciences
Luke Lear, Dan Padfield, Elze Hesse, Suzanne Kay, Angus Buckling, Michiel Vos
Summary: Metal pollution may decrease virulence in bacterial communities, but at a cost to diversity and productivity.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Horticulture
Xiangli Sun, Zebin Yuan, Bo Wang, Liping Zheng, Jianzhong Tan
Summary: This study demonstrated that exogenous putrescine (Put) supplementation can enhance the synthesis of endogenous arginine (Arg) and polyamines (PAs), while reducing the decomposition rate of PAs in Anthurium andraeanum seedlings under chilling stress. On the other hand, supplementation of D-arginine (D-Arg) and/or difluoromethylornithine (DFMO) inhibited the synthesis of endogenous Arg and its conversion to Put, accelerating the decomposition of PAs. Transcriptional analysis confirmed the differential expression of genes involved in these pathways, supporting the observed physiological effects.
SCIENTIA HORTICULTURAE
(2021)
Article
Oncology
Yue Liu, Kai Fu, Eric M. Wier, Yifan Lei, Andrea Hodgson, Dongqing Xu, Xue Xia, Dandan Zheng, Hua Ding, Cynthia L. Sears, Jian Yang, Fengyi Wan
Summary: This study identified a novel genotoxin called UshA in attaching/effacing pathogens, which triggers DNA damage and initiates tumorigenic transformation during bacterial infections. Furthermore, it was found that UshA plays a critical role in accelerating colon tumorigenesis in mice. These findings highlight the importance of UshA in the development of colon cancer caused by bacterial infections.
Article
Microbiology
Te Liu, Yuying Liu, Zixuan Bu, Fan Yin, Yongqing Zhang, Jinjin Liu, Shaowen Li, Chen Tan, Xiabing Chen, Lu Li, Rui Zhou, Qi Huang
Summary: This study reveals the importance of the Tat system for bacterial motility in ExPEC, highlighting the role of transcriptional regulation in mediating motility defects, identifying Tat substrate proteins responsible for nonmotile phenotype, and showing the contribution of the Rcs system in suppressing motility.
JOURNAL OF BACTERIOLOGY
(2022)
Review
Cell Biology
Akanksha Sharma, Hema Kumari Alajangi, Giuseppina Pisignano, Vikas Sood, Gurpal Singh, Ravi Pratap Barnwal
Summary: The survival of microorganisms depends on environmental conditions and host compatibility. Microorganisms adopt specific strategies to thrive in their surroundings and evade host defenses, with RNA-based regulatory mechanisms being the most common ones in response to environmental changes and host invasion.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2022)
Article
Microbiology
Yu Shi, Qin Cao, Jingdu Sun, Xiaofang Hu, Zhi Su, Yongchang Xu, Huimin Zhang, Lefu Lan, Youjun Feng
Summary: In this study, the function of Pseudomonas aeruginosa BioH protein in the biotin synthesis pathway was defined. The protein allows biotin to exit the fatty acid synthesis cycle and enter the late stage of biotin synthesis. The study also found significant differences in biotin abundance in mouse tissues and organs. BioH was found to be important for the infectivity of Pseudomonas aeruginosa, and the biotin synthesis inhibitor MAC13772 can interfere with the production of a virulence factor.
Article
Biology
Sarah O'Keefe, Guanghui Zong, Kwabena B. Duah, Lauren E. Andrews, Wei Q. Shi, Stephen High
Summary: The integration of type III single-pass transmembrane proteins targeted to the ER and the roles of the ER membrane complex, Sec61 complex and signal recognition particle receptor were investigated by O'Keefe et al. The authors proposed an alternative mechanism of insertion, reconciling the ability of type III transmembrane proteins to bypass an ipomoeassin-F-mediated blockade of membrane integration.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mar Perez-Ruiz, Mar Pulido-Cid, Juan Roman Luque-Ortega, Jose Maria Valpuesta, Ana Cuervo, Jose L. Carrascosa
Summary: The passage of viral DNA in bacteriophage T7 involves the assembly of core proteins gp15 and gp16 into a tube structure within the periplasm, allowing DNA transfer. The interaction between these proteins results in the formation of a channel for DNA translocation and also involves a transglycosylase motif for peptidoglycan layer degradation. This complex matches the structure of the phage tail and proposes a model for the assembly of the core translocation complex, elucidating the molecular mechanism of T7 viral DNA release into the bacterial cytoplasm.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Evelien De Waelheyns, Kenneth Segers, Marios Frantzeskos Sardis, Jozef Anne, Gerry A. F. Nicolaes, Anastassios Economou
JOURNAL OF ANTIBIOTICS
(2015)
Article
Biotechnology & Applied Microbiology
Christian Rueckert, Andreas Albersmeier, Tobias Busche, Sebastian Jaenicke, Anika Winkler, Olafur H. Friojonsson, Guomundur Oli Hreggviosson, Christophe Lambert, Daniel Badcock, Kristel Bernaerts, Jozef Anne, Anastassios Economou, Joern Kalinowski
JOURNAL OF BIOTECHNOLOGY
(2015)
Article
Biology
Jeroen Bouvin, Simon Cajot, Pieter-Jan D'Huys, Jerry Ampofo-Asiama, Jozef Anne, Jan Van Impe, Annemie Geeraerd, Kristel Bernaerts
MATHEMATICAL BIOSCIENCES
(2015)
Article
Biotechnology & Applied Microbiology
Mohamed Belal Hamed, Spyridoula Karamanou, Solveig Olafsdottir, Joana Sofia Martins Basilio, Kenneth Simoens, Kostantinos C. Tsolis, Lieve Van Mellaert, Eik Elisabet Guomundsdottir, Gudmundur Oli Hreggvidsson, Jozef Anne, Kristel Bernaerts, Olafur H. Fridjonsson, Anastassios Economou
MICROBIAL CELL FACTORIES
(2017)
Article
Biochemistry & Molecular Biology
Marisol Roldan-Tapia, Jozef Anne, Ana Gisela Reyes, Ulises Carrasco, Cesar Millan-Pacheco, Javier Barrios-Gonzalez, Armando Mejia
PROTEIN AND PEPTIDE LETTERS
(2017)
Article
Biotechnology & Applied Microbiology
Konstantinos C. Tsolis, Evridiki-Pandora Tsare, Georgia Orfanoudaki, Tobias Busche, Katerina Kanaki, Reshmi Ramakrishnan, Frederic Rousseau, Joost Schymkowitz, Christian Rueckert, Joern Kalinowski, Jozef Anne, Spyridoula Karamanou, Maria I. Klapa, Anastassios Economou
MICROBIAL CELL FACTORIES
(2018)
Review
Biochemistry & Molecular Biology
Smitha C. Rao, Evelien De Waelheyns, Anastassios Economou, Jozef Anne
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2014)
Review
Biochemistry & Molecular Biology
Jozef Anne, Kristof Vrancken, Lieve Van Mellaert, Jan Van Impe, Kristel Bernaerts
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2014)
Article
Evolutionary Biology
Pablo Cruz-Morales, Erik Vijgenboom, Fernanda Iruegas-Bocardo, Genevieve Girard, Luis Alfonso Yanez-Guerra, Hilda E. Ramos-Aboites, Jean-Luc Pernodet, Jozef Anne, Gilles P. van Wezel, Francisco Barona-Gomez
GENOME BIOLOGY AND EVOLUTION
(2013)
Article
Engineering, Biomedical
Annabel Braem, Lieve Van Mellaert, Tina Mattheys, Dorien Hofmans, Evelien De Waelheyns, Liesbet Geris, Jozef Anne, Jan Schrooten, Jef Vleugels
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2014)
Article
Biotechnology & Applied Microbiology
Pieter-Jan D'Huys, Ivan Lule, Dominique Vercammen, Jozef Anne, Jan F. Van Impe, Kristel Bernaerts
JOURNAL OF BIOTECHNOLOGY
(2012)
Article
Biotechnology & Applied Microbiology
Jozef Anne, Barbara Maldonado, Jan Van Impe, Lieve Van Mellaert, Kristel Bernaerts
JOURNAL OF BIOTECHNOLOGY
(2012)
Article
Biochemical Research Methods
Julio C. Ayala, Elsa Pimienta, Caridad Rodriguez, Jozef Anne, Carlos Vallin, Maria T. Milanes, Emmanuel King-Batsios, Kris Huygen, Lieve Van Mellaert
JOURNAL OF MICROBIOLOGICAL METHODS
(2013)
Article
Biology
Ivan Lule, Pieter-Jan D'Huys, Lieve Van Mellaert, Jozef Anne, Kristel Bernaerts, Jan Van Impe
MATHEMATICAL BIOSCIENCES
(2013)
Article
Biotechnology & Applied Microbiology
Joachim Koepff, Matthias Keller, Konstantinos C. Tsolis, Tobias Busche, Christian Rueckert, Mohamed B. Hamed, Jozef Anne, Joern Kalinowski, Wolfgang Wiechert, Anastassios Economou, Marco Oldiges
BIOTECHNOLOGY AND BIOENGINEERING
(2017)