Article
Microbiology
Madeleine C. Stone, Aaron Mychack, Kathryn A. Coe, Suzanne Walker
Summary: The study profiled the effects of the arylomycin derivative G0775 on Staphylococcus aureus using transposon sequencing. The researchers identified resistance mechanisms and targets for synergism, including the lipoprotein processing pathway. In addition, upregulation of certain genes was found to be protective against G0775.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Review
Physiology
Sharbani Kaushik, Haoze He, Ross E. Dalbey
Summary: This review summarizes the crucial role of signal peptides in protein export and membrane protein insertion, as well as their function as key factors for protein targeting and translocation machinery activation. The importance of signal peptide removal and degradation, as well as the emerging research on signal peptidases as novel targets for antibiotic development, are also discussed.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biology
William J. Allen, Robin A. Corey, Daniel W. Watkins, A. Sofia F. Oliveira, Kiel Hards, Gregory M. Cook, Ian Collinson
Summary: Transport of proteins across and into membranes is a fundamental process in biology. Recent studies have shown that the diffusion of arginine residues limits pre-protein transport, while lysine can be transported across membranes in its neutral form. These findings have implications for understanding the mechanism of protein secretion and suggest a potential role of proton-motive force in aiding transport.
Editorial Material
Biology
Carolina Oliveira de Santana, Pieter Spealman, Gabriel G. Perron
Summary: The global spread of antibiotic resistance may be caused by various factors, not only the previous assumption of antibiotic overuse in agriculture and medicine.
Article
Microbiology
Rocio Aguilar Suarez, Minia Antelo-Varela, Sandra Maass, Jolanda Neef, Dorte Becher, Jan Maarten van Dijl
Summary: Genome engineering allows for the creation of novel cell factories with enhanced properties, as shown with Bacillus subtilis, where genome minimization has led to strains with beneficial traits for protein production. However, excessively minimized genomes can cause growth defects. The strain midiBacillus, which lacks 30.95% of the parental genome, demonstrates improved protein secretion responses compared to the wild-type strain.
Article
Biochemistry & Molecular Biology
Joanna E. Musik, Yaramah M. Zalucki, Christopher J. Day, Michael P. Jennings
Summary: This study identified the crucial role of the signal peptidase SipW in processing proteins TapA and TasA in Bacillus subtilis. Experimental fusion of the TasA signal peptide with other proteins revealed that certain residues around the predicted cleavage site inhibit the activity of Escherichia coli signal peptidase I, LepB, leading to cell death. The findings suggest that the cell death observed is likely due to the blocking of LepB by the TasA signal peptide, hindering the general secretion pathway.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Review
Pharmacology & Pharmacy
Christopher Schwake, Michael Hyon, Athar H. Chishti
Summary: Parasitic and viral infections pose a significant health and economic burden, with a lack of new therapeutics available. SPP has been identified as essential for malaria parasite growth, and evidence suggests its importance as a therapeutic target for other parasites. In the face of drug-resistant parasites and viruses, SPP inhibition offers potential for developing new compounds to combat antimicrobial resistance.
EXPERT OPINION ON THERAPEUTIC TARGETS
(2022)
Article
Microbiology
Y. Cherrak, I. Filella-Merce, V. Schmidt, D. Byrne, V. Sgoluppi, R. Chaiaheloudjou, S. Betzi, X. Morelli, M. Nilges, R. Pellarin, E. Durand
Summary: The increasing drug-resistance of bacterial infections poses a threat to human health. Targeting the T6SS virulence factor as a potential strategy to combat pathogens, particularly antibiotic-resistant species, is a promising approach. The development of T6SS inhibitors could provide new therapeutic options to fight drug-resistant infections.
Article
Multidisciplinary Sciences
Anne-Bart Seinen, Dian Spakman, Antoine M. van Oijen, Arnold J. M. Driessen
Summary: SecA predominantly associates with the cytoplasmic membrane as a homodimer in three distinct diffusional populations. Disruption of the proton-motive-force results in the re-localization of SecA to the cytoplasm and transient location at specific membrane sites. This supports a model in which SecA diffuses along the membrane surface to access the SecYEG translocon.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Catherine Shelton, Matthew McNeil, Renee Allen, Lindsay Flint, Dara Russell, Bryan Berube, Aaron Korkegian, Yulia Ovechkina, Tanya Parish
Summary: By studying the mutations in specific genes of Mycobacterium tuberculosis, we found that triazolopyrimidines can interact with these genes and confer resistance to the bacteria. These compounds deplete intracellular ATP levels and exhibit activity against intracellular bacteria, but have no effect on human mitochondrial respiration.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Joanna E. Musik, Jessica Poole, Christopher J. Day, Thomas Haselhorst, Freda E. -C. Jen, Thomas Ve, Veronika Masic, Michael P. Jennings, Yaramah M. Zalucki
Summary: This study designed 11 peptides to mimic signal peptides that are inefficiently cleaved by LepB. The binding affinity and inhibitory potential of these peptides against LepB were assessed. It was found that the tryptophan residue in the signal peptide inhibited the cleavage of the peptide by LepB, and replacing this residue with alanine improved the cleavage efficiency.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biology
Manuel Strach, Felicitas Koch, Svenja Fiedler, Klaus Liebeton, Peter L. Graumann
Summary: Research on the secretion of alpha-amylase AmyE in Bacillus strains revealed that the protein may be released through a combination of active transport across the cell membrane and slow, passive diffusion through the cell wall. The existence of secretion zones during the transition to and within the stationary phase indicates a dynamic process of protein secretion in Gram-positive bacteria.
Article
Infectious Diseases
Hak Jin Lee, Seong Tae Jhang, Hyung Jong Jin
Summary: Resistance to Macrolide-lincosamide-streptogramin B antibiotics is mainly caused by the action of erythromycin ribosome methylation proteins. Research on the conservation region of Erms may lead to the development of inhibitors. The size and chemical properties of side chains in mutants significantly affect their activity.
Article
Microbiology
T. A. Alsenani, S. L. Viviani, K. M. Papp-Wallace, R. A. Bonomo, F. van den Akker
Summary: KPC-2 is a bacterial strain that is resistant to carbapenem drugs and recent variants have developed resistance to other drugs as well. This study focuses on the structural changes in KPC-2 D179N when bound to avibactam and relebactam, providing insight into the decreased affinity of D179Y variant to avibactam and potential design of novel beta-lactamase inhibitors.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Giang Ngo, Melis Girbas, Hannah Schaetzle, Andreas Hammer, Schara Safarian, Maximilian Huebinger, Enrico Schleiff
Summary: This study investigated the function and genomic characteristics of two outer membrane transporters, TpsB1 and TpsB2, in Anabaena sp. strain PCC 7120, revealing their roles in substrate transport and membrane integrity in cyanobacteria. Experimental and bioinformatics analyses suggested a crucial function of these proteins in cyanobacterial physiology.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jozefien De Geyter, Athina G. Portaliou, Bindu Srinivasu, Srinath Krishnamurthy, Anastassios Economou, Spyridoula Karamanou
Article
Clinical Neurology
Xiaohang Li, Konstantinos C. Tsolis, Marta J. Koper, Alicja Ronisz, Simona Ospitalieri, Christine A. F. von Arnim, Rik Vandenberghe, Thomas Tousseyn, Angelika Scheuerle, Anastassios Economou, Sebastien Carpentier, Markus Otto, Dietmar Rudolf Thal
Summary: Proteome profile changes in Alzheimer's disease brains involve early-responding, late-responding, gradually-changing, and fraction-shifting proteins. Gene ontology analysis indicates vesicle endocytosis and the secretory pathway as early-involved components in AD.
ALZHEIMERS & DEMENTIA
(2021)
Article
Biochemistry & Molecular Biology
Srinath Krishnamurthy, Nikolaos Eleftheriadis, Konstantina Karathanou, Jochem H. Smit, Athina G. Portaliou, Katerina E. Chatzi, Spyridoula Karamanou, Ana-Nicoleta Bondar, Giorgos Gouridis, Anastassios Economou
Summary: The interaction between cytoplasmic ATPase SecA and membrane-embedded SecYEG channel in forming the Sec translocase involves the utilization of intrinsic dynamics in SecA. These dynamics elements play a crucial role in regulating catalytic activity and clamp motions. A slender stem in SecA acts as a nexus, biasing the rotation of the preprotein clamp through ATPase domain motions.
Article
Microbiology
Julian Droste, Christian Rueckert, Jorn Kalinowski, Mohamed Belal Hamed, Jozef Anne, Kenneth Simoens, Kristel Bernaerts, Anastassios Economou, Tobias Busche
Summary: Through transcriptome and proteome data analysis, the study improved the annotation of the S. lividans TK24 genome, revealing insights into promoter structure, 5'-UTRs, cis-regulatory elements, attenuator structures, and novel transcripts. The genome of S. lividans TK24 contains a vast amount of transcriptional information on secondary metabolite gene clusters, laying the foundation for future research on gene characterization and transcriptional regulatory networks.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Mohamed Belal Hamed, Ewa Burchacka, Liselotte Angus, Arnaud Marchand, Jozefien De Geyter, Maria S. Loos, Jozef Anne, Hugo Klaassen, Patrick Chaltin, Spyridoula Karamanou, Anastassios Economou
Summary: The study identified 14 secretion inhibitors with potential antibacterial activity through high-throughput screening, some of which demonstrated bactericidal effects against both Gram-negative and Gram-positive bacteria. These results provide evidence for the development of potential antibiotics starting compounds.
Article
Chemistry, Analytical
Jochem H. Smit, Srinath Krishnamurthy, Bindu Y. Srinivasu, Rinky Parakra, Spyridoula Karamanou, Anastassios Economou
Summary: HDX-MS is a powerful technique for monitoring protein intrinsic dynamics, but analyzing the data sets can be challenging. PyHDX is an open-source Python package and webserver that allows users to batch extract the universal quantity Gibbs free energy at residue levels across multiple protein conditions and homologues.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Aspasia Destouni, Konstantinos C. Tsolis, Anastassios Economou, Ioanna Papathanasiou, Charalampos Balis, Evanthia Mourmoura, Aspasia Tsezou
Summary: A systems analysis approach identified modules related to extracellular matrix mechanosensing and glycolysis in the core proteome network of chondrocytes, showing strong correlation with osteoarthritis. The interconnection between cartilage ECM remodeling and metabolism indicates the significance of dynamic chondrocyte states in osteoarthritis.
EXPERT REVIEW OF PROTEOMICS
(2021)
Editorial Material
Multidisciplinary Sciences
Jochem H. Smit, Guillaume Roussel, Anastassios Economou
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Analytical
Juergen Claesen, Srinath Krishnamurthy, Andy M. Lau, Anastassios Economou
Summary: This study introduces a method of using a linear model combined with an empirical Bayes approach to detect differentially deuterated peptides, in order to study the structure and dynamics of protein states. By moderating the two-sample t-tests and F-statistic, the number of false positive and false negative findings can be reduced.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Nicolas Rosa, Hristina Ivanova, Larry E. Wagner, Justin Kale, Rita La Rovere, Kirsten Welkenhuyzen, Nikolaos Louros, Spyridoula Karamanou, Victoria Shabardina, Irma Lemmens, Elien Vandermarliere, Kozo Hamada, Hideaki Ando, Frederic Rousseau, Joost Schymkowitz, Jan Tavernier, Katsuhiko Mikoshiba, Anastassios Economou, David W. Andrews, Jan B. Parys, David Yule, Geert Bultynck
Summary: The study shows that Bcl-xL and Bcl-2 have similar anti-apoptotic effects in controlling endoplasmic reticulum Ca2+ release, challenging the current understanding of their divergent functions in Ca2+ signaling modulation.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Biochemistry & Molecular Biology
Biao Yuan, Athina G. Portaliou, Rinky Parakra, Jochem H. Smit, Jiri Wald, Yichen Li, Bindu Srinivasu, Maria S. Loos, Harveer Singh Dhupar, Dirk Fahrenkamp, Charalampos G. Kalodimos, Franck Duong van Hoa, Thorben Cordes, Spyridoula Karamanou, Thomas C. Marlovits, Anastassios Economou
Summary: Type III protein secretion is common in Gram-negative pathogens, with SctV forming peripheral oligomeric clusters in EPEC and serving as a key receptor for different chaperone/exported protein pairs. The dynamic motions of SctV protomers, modulated by chaperones and ATPase, may allosterically affect the secretion process.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Giorgos Gouridis, Yusran A. Muthahari, Marijn de Boer, Douglas A. Griffith, Alexandra Tsirigotaki, Konstantinos Tassis, Niels Zijlstra, Ruixue Xu, Nikolaos Eleftheriadis, Yovin Sugijo, Martin Zacharias, Alexander Domling, Spyridoula Karamanou, Charalambos Pozidis, Anastassios Economou, Thorben Cordes
Summary: Novel biophysical tools have allowed exploration of protein structural dynamics and evolution, revealing how modifications of structural cores can lead to diversification of protein functions. The mechanism uncovered in this study provides insights into the emergence of functional promiscuity during long periods of evolution, with implications for a wide range of domain architectures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Srinath Krishnamurthy, Marios-Frantzeskos Sardis, Nikolaos Eleftheriadis, Katerina E. Chatzi, Jochem H. Smit, Konstantina Karathanou, Giorgos Gouridis, Athina G. Portaliou, Ana-Nicoleta Bondar, Spyridoula Karamanou, Anastassios Economou
Summary: Protein machines undergo conformational motions to interact with and manipulate polymeric substrates. The intrinsic dynamics of Sec translocase ATPase, SecA, and the binding of preproteins combine to achieve translocation. This universal mechanism allows any preprotein to undergo translocation and be secreted.
Article
Biochemistry & Molecular Biology
Dries Smets, Jochem Smit, Ying Xu, Spyridoula Karamanou, Anastassios Economou
Summary: Signal peptides delay folding by crosstalking with mature domains, altering the folding landscape of proteins.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Dries Smets, Alexandra Tsirigotaki, Jochem H. Smit, Srinath Krishnamurthy, Athina G. Portaliou, Anastassia Vorobieva, Wim Vranken, Spyridoula Karamanou, Anastassios Economou
Summary: This study investigates the delayed folding mechanism and the regulatory role of signal peptides in the Sec pathway. By comparing two homologous proteins, PpiA and PpiB, it is found that PpiA folds slower and the folding process involves hierarchical foldons. The folding of PpiA is delayed by less hydrophobic native contacts, frustrated residues, and a beta-turn in the earliest foldon, as well as by signal peptide-mediated disruption of foldon hierarchy. Additionally, grafting selected residues or the signal peptide of PpiA onto PpiB converts it into a slow folder with enhanced in vivo secretion. These structural adaptations facilitate protein trafficking.
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)