Article
Medicine, General & Internal
Guannan Zhou, Yuanyuan Gu, Zhongyi Zhu, Hongdao Zhang, Wei Liu, Beiying Xu, Fangyue Zhou, Menglei Zhang, Keqin Hua, Ligang Wu, Jingxin Ding
Summary: This study discovered that exosomes can deliver cisplatin through clathrin-independent endocytosis, avoiding endosome trapping and evenly distributing in the cytosol. The research clarifies the mechanism of exosome-mediated drug delivery against cisplatin-resistant cancer and suggests that exosomes can be a potential nano-carrier for treating cisplatin-resistant ovarian cancer.
FRONTIERS IN MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Yuzy Fauzyah, Chikako Ono, Shiho Torii, Itsuki Anzai, Rigel Suzuki, Takuma Izumi, Yuhei Morioka, Yusuke Maeda, Toru Okamoto, Takasuke Fukuhara, Yoshiharu Matsuura
Summary: Discovery of a potential anti-HBV drug, ponesimod, targeting endosome maturation of HBV, through drug screening of GPCR-associated compound library. Ponesimod significantly reduces HBV rcDNA level at early infection step and interferes with conversion of early endosomes to late endosomes during HBV transportation to the nucleus.
ANTIVIRAL RESEARCH
(2021)
Article
Genetics & Heredity
Anne Norris, Collin T. McManus, Simon Wang, Ruochen Ying, Barth D. Grant
Summary: This study reveals the mechanism by which the recycling protein RME-8 regulates microdomain segregation in endosomes. We found that RME-8 is inhibited on endosomal membranes by homo-oligomerization until it is activated by companion recycling protein SNX-1. This activation allows RME-8 to uncoat both degradative sorting proteins and its own activator SNX-1, limiting the range of microdomains and providing negative feedback regulation. The results suggest that RME-8 and SNX-1 play important roles in an expansive recycling machinery, and there is still much exciting work to be done to uncover their functions within this broader context.
Review
Neurosciences
Daniela Ivanova, Michael A. Cousin
Summary: The endolysosomal system plays a crucial role in cells and inter-neuronal communication. It has been found that molecules involved in endolysosomal function are also important for the recycling of neurotransmitter-containing synaptic vesicles. Therefore, a rethinking of the understanding of membrane trafficking at the presynapse is proposed.
FRONTIERS IN SYNAPTIC NEUROSCIENCE
(2022)
Review
Genetics & Heredity
Chung-Ling Lu, Jinoh Kim
Summary: Cells utilize membrane-bound carriers to transport signaling molecules to their destinations, and variations in the genes encoding intracellular trafficking machinery can lead to craniofacial diseases. Despite the fundamental importance of trafficking pathways in craniofacial morphogenesis, there has been relatively less emphasis on this topic so far.
Article
Oncology
Suree Kim, Sukyeong Heo, Joseph Brzostowski, Dongmin Kang
Summary: This study focuses on the full activation of AKT, a key effector in PI3K-driven cell signal transduction, through phosphorylation at Thr308 and Ser473. It explores the mechanisms of AKT activation at early endosomes during growth factor stimulation, with a specific focus on the role of mTORC2 in this process. The research demonstrates the importance of endocytosis in AKT activation through mTORC2 phosphorylation at Ser473 and the involvement of endosomal phosphoinositide, PtdIns(3,4)P-2, in this signaling pathway.
Article
Neurosciences
Melissa Plooster, Guendalina Rossi, Martilias S. Farrell, Jessica C. McAfee, Jessica L. Bell, Michael Ye, Graham H. Diering, Hyejung Won, Stephanie L. Gupton, Patrick Brennwald
Summary: TSNARE1, a high-confidence gene candidate for schizophrenia risk, regulates endosomal trafficking in cortical neurons and may act as an inhibitory SNARE by competing for incorporation into endosomal SNARE complex. Its localization in endosomal network and late endosomes suggests a role in negative regulation of early endosomal to late endosomal trafficking.
JOURNAL OF NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Anna Ciesielska, Marta Matyjek, Katarzyna Kwiatkowska
Summary: This article discusses the role of TLR4 in promoting inflammatory responses, including internalization and intracellular trafficking of exogenous endotoxin and bacteria. The authors emphasize the significant impact of internalization rate and endo-lysosomal compartment transport rate on inflammatory responses.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Cell Biology
Awatef Ben Ahmed, Quentin Lemaire, Jodie Scache, Christophe Mariller, Tony Lefebvre, Anne-Sophie Vercoutter-Edouart
Summary: The transport of proteins in cells is regulated by the secretory pathway, as well as unconventional secretion pathways. These complex processes rely on various signaling and regulatory proteins to ensure proper delivery of cargoes. O-GlcNAcylation, a reversible post-translational modification, plays a role in protein trafficking in mammalian cells through classical and unconventional secretory pathways. This review summarizes the emerging role of O-GlcNAc modification in protein trafficking.
Article
Multidisciplinary Sciences
Bjoern U. Klink, Eric Herrmann, Claudia Antoni, Lars Langemeyer, Stephan Kiontke, Christos Gatsogiannis, Christian Ungermann, Stefan Raunser, Daniel Kuemmel
Summary: This study presents a high-resolution cryogenic electron microscopy structure of the Mon1-Ccz1 complex, revealing its strong scaffold for the catalytic center and the phosphatidylinositol phosphate-binding site, providing molecular insights into endosomal Rab activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Xue-hai Liang, Joshua G. Nichols, Dario Tejera, Stanley T. Crooke
Summary: Studies have shown that the distribution and activity of PS-ASO in cells are affected by endosome movement and perinuclear positioning. PS-ASO moves linearly along microtubules and covers substantial distances in late endosomes, while perinuclear positioning is necessary for efficient release of PS-ASO.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Julien Bous, Aurelien Fouillen, Helene Orcel, Stefano Trapani, Xiaojing Cong, Simon Fontanel, Julie Saint-Paul, Josephine Lai-Kee-Him, Serge Urbach, Nathalie Sibille, Remy Sounier, Sebastien Granier, Bernard Mouillac, Patrick Bron
Summary: Recent structural studies have provided insights into the molecular mechanisms of GPCR-arrestin coupling, but it is unclear whether this process is conserved among different GPCRs. In this study, the cryo-electron microscopy active structure of the wild-type arginine-vasopressin V2 receptor (V2R) in complex with beta-arrestin1 was determined, revealing a unique arrangement of beta-arrestin1 and an interface involving all receptor intracellular loops. These findings suggest a significant structural variability among GPCR-arrestin signaling complexes.
Article
Biochemistry & Molecular Biology
Naava Naslavsky, Steve Caplan
Summary: This Viewpoint focuses on the importance of endosomes in cells and the underlying mechanisms of their functions.
Article
Biology
Junko Y. Toshima, Ayana Tsukahara, Makoto Nagano, Takuro Tojima, Daria E. Siekhaus, Akihiko Nakano, Jiro Toshima, Sean Munro
Summary: Budding yeast possesses an independent endocytic early/sorting compartment within the trans-Golgi network (TGN) that sorts endocytic cargos. This compartment functions as the target for endocytic vesicles and is involved in recycling to the plasma membrane or transport to the Rab5 endosomal compartment. The Tlg2p protein, a homolog of yeast syntaxin, resides in this compartment and acts as a Q-SNARE for endocytic vesicles.
Article
Environmental Sciences
Ignacio Babiloni-Chust, Reinaldo S. dos Santos, Regla M. Medina-Gali, Atenea A. Perez-Serna, Jose-Antonio Encinar, Juan Martinez-Pinna, Jan-Ake Gustafsson, Laura Marroqui, Angel Nadal
Summary: 17 beta-estradiol protects pancreatic beta-cells from apoptosis via estrogen receptors ER alpha, ER beta, and GPER. Conversely, the endocrine disruptor bisphenol-A (BPA) increases basal apoptosis in the same cell type through the same estrogen receptors. The molecular events that trigger these opposite actions have not been identified yet.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Neurosciences
Elizabeth M. Waters, Louisa I. Thompson, Parth Patel, Andreina D. Gonzales, Hector (Zhiyu) Ye, Edward J. Filardo, Deborah J. Clegg, Jolanta Gorecka, Keith T. Akama, Bruce S. McEwen, Teresa A. Milner
JOURNAL OF NEUROSCIENCE
(2015)
Article
Cell Biology
H. M. Gaudet, S. B. Cheng, E. M. Christensen, E. J. Filardo
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2015)
Review
Biochemistry & Molecular Biology
Edward J. Filardo
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Matthias Barton, Edward J. Filardo, Stephen J. Lolait, Peter Thomas, Marcello Maggiolini, Eric R. Prossnitz
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2018)
Article
Endocrinology & Metabolism
Anne Almey, Edward J. Filardo, Teresa A. Milner, Wayne G. Brake
Review
Endocrinology & Metabolism
Edward J. Filardo, Peter Thomas
Article
Endocrinology & Metabolism
Anne Almey, Elizabeth Cannell, Kyla Bertram, Edward Filardo, Teresa A. Milner, Wayne G. Brake
Article
Biochemistry & Molecular Biology
Shi-Bin Cheng, Jeffrey A. Quinn, Carl T. Graeber, Edward J. Filardo
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Biochemistry & Molecular Biology
Shi-Bin Cheng, Edward J. Filardo
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Zoology
Michelle M. Naugle, Long T. Nguyen, Tyler K. Merceron, Edward Filardo, William G. M. Janssen, John H. Morrison, Peter R. Rapp, Andrea C. Gore
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
(2014)
Article
Cell Biology
Shi-Bin Cheng, Jing Dong, Yefei Pang, Jessica LaRocca, Mary Hixon, Peter Thomas, Edward J. Filardo
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2014)
Article
Oncology
Hilary T. Magruder, Jeffrey A. Quinn, Jean E. Schwartzbauer, Jonathan Reichner, Allan Huang, Edward J. Filardo
Review
Endocrinology & Metabolism
Milad Rouhimoghadam, Anh S. Lu, Aliasger K. Salem, Edward J. Filardo
FRONTIERS IN ENDOCRINOLOGY
(2020)
Article
Medicine, Research & Experimental
Anh S. Lu, Milad Rouhimoghadam, Christopher K. Arnatt, Edward J. Filardo, Aliasger K. Salem
Summary: Decisions for hormonal therapy in breast cancer are based solely on ER presence, yet GPER is crucial. New chimeras UI-EP001 and UI-EP002 effectively degrade ERs and GPER, inducing cytotoxicity and cell cycle arrest in breast cancer cells. Targeting both plasma membrane and intracellular estrogen receptors could be a promising strategy for breast cancer treatment.
MOLECULAR PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Olexander M. Semenenko, Victoria V. Lipson, Alina O. Sadchenko, Olga V. Vashchenko, Natalia A. Kasian, Liliia Sviechnikova, Longin M. Lisetski, Mykola L. Babak, Volodymyr M. Vakula, Oleksandr Borysov, Yuliia Holota, Sergey O. Zozulya, Petro O. Borysko, Olexander Mazepa
Summary: An efficient protocol for the synthesis of novel methotrexate-betulonic acid hybrids has been developed and their structures and properties have been fully established. The hybrids showed inferior interactions with artificial lipid membranes, possibly due to their increasing lipophilicity.
Article
Biochemistry & Molecular Biology
Mohadese Yaghoobi Anzabi, Piotr Cmoch, Roman Luboradzki, Zbigniew Pakulski
Summary: Friedelin and 3-acetoxyfriedel-3-en-2-one, known as friedelane triterpenoids, have been isolated from cork smoker wash solids and used as starting materials for the synthesis of new friedelane derivatives. Reduction reactions result in the formation of friedelinol and epi-cerin derivatives.
Article
Biochemistry & Molecular Biology
Detlef Thieme, Aniko Krumbholz, Martin Bidlingmaier, Christoph Geffert, Annika Hameder, Andreas Stoever, Matthias Graw, Annekathrin M. Keiler
Summary: Ethanol can affect testosterone metabolism and potentially influence steroid biosynthesis and blood steroid profiles. The study emphasizes the importance of sufficient time interval from food and ethanol intake during blood sampling, particularly for diagnostics in doping control.
Article
Biochemistry & Molecular Biology
Masanobu Murao, Tetsuo Imano, Yoshinobu Sato, Masaaki Nakajima
Article
Biochemistry & Molecular Biology
Jingyuan Wang, Ye Feng, Brian Liu, Wen Xie
Summary: Sulfation and desulfation of steroids are opposing processes that regulate steroid homeostasis and are involved in steroid-related diseases. Cytosolic sulfotransferase and steroid sulfatase modify and regulate steroids to affect hormonal dysregulation, metabolic diseases, and cancer.
Article
Biochemistry & Molecular Biology
Noah K. Babel, Brian J. Feldman
Summary: Our research identified a previously unrecognized ADAMTS1-PTN-Wnt pathway in regulating adipose precursor cell behavior. The non-muscle myosin protein MYH9 was found to be a key target of this pathway for modulating adipogenesis. These findings offer potential for developing novel therapeutics for obesity and related metabolic disorders.
Article
Biochemistry & Molecular Biology
Martiniano Bello
Summary: This study explores the structural and thermodynamic basis of molecular recognition between ERβ and DPN and its derivatives using molecular dynamics simulations and end-point methods. The results show that the MMGBSA approach is able to reproduce experimental trends and identify eight residues that strongly interact with the ligands.