Article
Biochemistry & Molecular Biology
Abul K. Azad, Maikel A. Farhan, Cameron R. Murray, Kunimasa Suzuki, Gary Eitzen, Nicolas Touret, Ronald B. Moore, Allan G. Murray
Summary: Angiogenesis is essential in embryonic development and tissue repair. FGD5, an endothelial Rho GTP/GDP exchange factor, has been found to regulate CXCR4-dependent signals in endothelial cells and play a role in angiogenesis. Inhibition of FGD5 activity may complement current angiogenesis inhibitor drugs.
Article
Multidisciplinary Sciences
Wen Lu, Katarzyna M. Skrzypczynska, Arthur Weiss
Summary: Src-family kinases play different roles in T cell and B cell signaling, with their activity regulated by factors such as Csk. Csk is crucial for maintaining appropriate SFK activity levels and regulating PIP3 amounts to prevent inappropriate B cell activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Dermatology
Amy L. Harding, Helen E. Colley, Inmaculada Barragan Vazquez, Simon Danby, Md Zobaer Hasan, Hirofumi Nakanishi, Tetsuo Furuno, Craig Murdoch
Summary: Skin irritancy is a significant problem that affects millions of people worldwide. New testing methods using human skin equivalents have found evidence of specific activation of c-Src kinase in response to chemical irritants, suggesting the development of rapid screening methods for potential irritants.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Anesthesiology
Attila Keresztes, Keith Olson, Paul Nguyen, Marissa A. Lopez-Pier, Ryan Hecksel, Natalie K. Barker, Zekun Liu, Victor Hruby, John Konhilas, Paul R. Langlais, John M. Streicher
Summary: This study reveals a new function of opioid receptors as a negative feedback brake on opioid-induced analgesia. By using a selective antagonist for the mu and delta opioid receptors (MDOR), the researchers found that the antagonist could enhance the analgesic effect of oxymorphone in mice, suggesting a role for MDOR in regulating pain. Additionally, the study found that the enhancement effect was selective to MDOR and occurred through the repression of Src and CaMKII signal transduction. These findings suggest that MDOR antagonism could be a potential approach to improve clinical opioid therapy.
Article
Cell Biology
Ziqian Shen, Abby Hinson, Richard A. Miller, Gonzalo G. Garcia
Summary: The study suggests that rapamycin and acarbose can prevent age-related declines in cap-independent translation (CIT) target proteins in both sexes, while 17 alpha-estradiol has a similar effect only in males. The activity of mTORC1 is blocked by rapamycin, acarbose, and 17 alpha-estradiol in age-related increases. The increase in METTL3 activity and CIT-dependent proteins may represent a shared pathway for both long-lived mutant mice and drug-induced lifespan extension in mice.
Article
Cell Biology
Xue-Hua Du, Shao-Bo Ke, Xin-Yi Liang, Jie Gao, Xiao-Xiao Xie, Lin-Zhi Qi, Xue-Yi Liu, Guo-Yuan Xu, Xiao-Dong Zhang, Run-Lei Du, Shang-Ze Li
Summary: Our study demonstrates that USP14 functions as a deubiquitinase that interacts and stabilizes JNK, promoting MAPK/JNK signaling and colorectal carcinogenesis. Increased expression of USP14 is associated with elevated levels of JNK protein and downstream gene expression in colorectal cancer patients. Inhibition of USP14 reduces cancer cell proliferation and tumorigenesis by downregulating the activation of the MAPK/JNK pathway.
CELL DEATH & DISEASE
(2023)
Review
Biochemistry & Molecular Biology
Aziz M. Rangwala, Victoria R. Mingione, George Georghiou, Markus A. Seeliger
Summary: Phosphorylation plays a vital role in regulating biological processes, and protein kinases have been extensively studied for their involvement in human health and disease. Some kinases possess additional catalytic functions in addition to phosphotransferase activity, while others have lost their catalytic activity completely. In this study, we analyzed the UniProtKB database for bifunctional protein kinases and focused on those crucial for bacterial and human cellular homeostasis. These kinases have diverse functional roles in environmental sensing, metabolic regulation, immune-host defense, and cell cycle control. This article explores their dual catalytic activities and their contributions to disease pathogenesis.
Article
Chemistry, Multidisciplinary
Zahra Niavarani, Daniel Breite, Andrea Prager, Isabell Thomas, Mathias Kuehnert, Bernd Abel, Roger Glaeser, Agnes Schulze
Summary: Composite microfiltration polyethersulfone membranes with molecularly imprinted particles (MIPs) were developed for efficient and selective adsorption of 17β-estradiol from water. The membranes exhibited significant adsorption loading and high selectivity for 17β-estradiol, even in the presence of other micropollutants. The membranes also showed excellent reusability and a maximum adsorption capacity more than 200 times higher than commercial nanofiltration.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Review
Cell Biology
Horacio Maldonado, Lisette Leyton
Summary: Cancer progression and metastasis are controlled by the integrin receptor family and their interaction with Src family kinases (SFKs). The non-receptor tyrosine kinase CSK suppresses SFK activity and may play a crucial role in inhibiting SFK oncogenic effects in cancer. CSK can localize near focal adhesions and interact with focal adhesion components, suggesting a role in regulating focal adhesion dynamics and structure. The specific role of CSK and its crosstalk with integrins in cancer progression, particularly in mechanosensing, remains unclear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemical Research Methods
Jun-Seong Ahn, Chang-Hyun Jang
Summary: An LC-based aptasensor has been developed to detect 17 beta-estradiol (E2) at picomolar levels by utilizing competitive reactions and interfacial events. The aptasensor changes the optical response of liquid crystals (LCs) through electrostatic interactions with a cationic surfactant (CTAB) and the aptamer. The conformational change of the aptamer due to binding with E2 weakens the electrostatic attraction, resulting in a dark optical image. The E2 sensor showed a detection limit of 3.1 pM in TBS and 6.8 pM in human urine, demonstrating high sensitivity and selectivity without the need for complex analysis equipment.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Jifeng Feng, Dihong Tang, Jing Wang, Qi Zhou, Jin Peng, Hanmei Lou, Yuping Sun, Yunlang Cai, Hongmin Chen, Junqin Yang, Pan Liu, Linna Wang, Jianjun Zou
Summary: SHR-1701 exhibits promising antitumor activity and controllable safety in patients with recurrent or metastatic cervical cancer after platinum-based regimens, providing another treatment option for this population.
CLINICAL CANCER RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Jasmine Fusi, Maria Cristina Veronesi, Alberto Prandi, Monica Probo, Massimo Faustini, Tanja Peric
Summary: This study investigated the changes in sexual hormones in dogs during puberty using hair and nail samples to analyze the concentrations of testosterone (T), 17 beta-estradiol (E2), and progesterone (P4). Traditional methods using blood samples are not suitable for long-term studies, but hair and nails have been identified as suitable retrospective biological specimens. The results showed significant increases in T levels in male dogs and E2 levels at puberty and P4 levels after puberty in female dogs. Hair and nail T and nail P4 concentrations could differentiate between male and female dogs at specific sampling times. This study demonstrates the usefulness of hair and nails as biological specimens for studying the changes in T, E2, and P4 during the peripubertal period in dogs.
Article
Plant Sciences
Eshan Sharma, Akanksha Bhatnagar, Avantika Bhaskar, Susmita M. Majee, Martin Kieffer, Stefan Kepinski, Paramjit Khurana, Jitendra P. Khurana
Summary: The rice FB protein-coding gene OsFBX257 is differentially expressed under drought conditions and other abiotic stresses. It plays a significant role in modulating root architecture and improving drought stress tolerance in rice. OsFBX257 is a potential breeding target for alleviating drought stress-induced damage in rice.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Natasha S. Clayton, Millie Fox, Jose J. Vicente-Garcia, Courtney M. Schroeder, Trevor D. Littlewood, Jonathon Wilde, Kadalmani Krishnan, Murray J. B. Brown, Claire Crafter, Helen R. Mott, Darerca Owen
Summary: Activated Cdc42-associated kinase (ACK) phosphorylates PI3K regulatory subunits, promoting cell proliferation. ACK interacts with p85 alpha in nuclear-enriched cell fractions, facilitating dimerization of the regulatory subunits.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Antonio Rodriguez-Blazquez, Arturo Carabias, Alba Moran-Vaquero, Sergio de Cima, Juan R. Luque-Ortega, Carlos Alfonso, Peter Schuck, Jose Antonio Manso, Sandra Macedo-Ribeiro, Carmen Guerrero, Jose M. de Pereda
Summary: C3G activation is regulated by phosphorylation and interaction with Crk adaptor proteins, revealing a two-factor mechanism for tight control of its activity.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Olexander M. Semenenko, Victoria V. Lipson, Alina O. Sadchenko, Olga V. Vashchenko, Natalia A. Kasian, Liliia Sviechnikova, Longin M. Lisetski, Mykola L. Babak, Volodymyr M. Vakula, Oleksandr Borysov, Yuliia Holota, Sergey O. Zozulya, Petro O. Borysko, Olexander Mazepa
Summary: An efficient protocol for the synthesis of novel methotrexate-betulonic acid hybrids has been developed and their structures and properties have been fully established. The hybrids showed inferior interactions with artificial lipid membranes, possibly due to their increasing lipophilicity.
Article
Biochemistry & Molecular Biology
Mohadese Yaghoobi Anzabi, Piotr Cmoch, Roman Luboradzki, Zbigniew Pakulski
Summary: Friedelin and 3-acetoxyfriedel-3-en-2-one, known as friedelane triterpenoids, have been isolated from cork smoker wash solids and used as starting materials for the synthesis of new friedelane derivatives. Reduction reactions result in the formation of friedelinol and epi-cerin derivatives.
Article
Biochemistry & Molecular Biology
Detlef Thieme, Aniko Krumbholz, Martin Bidlingmaier, Christoph Geffert, Annika Hameder, Andreas Stoever, Matthias Graw, Annekathrin M. Keiler
Summary: Ethanol can affect testosterone metabolism and potentially influence steroid biosynthesis and blood steroid profiles. The study emphasizes the importance of sufficient time interval from food and ethanol intake during blood sampling, particularly for diagnostics in doping control.
Article
Biochemistry & Molecular Biology
Masanobu Murao, Tetsuo Imano, Yoshinobu Sato, Masaaki Nakajima
Article
Biochemistry & Molecular Biology
Jingyuan Wang, Ye Feng, Brian Liu, Wen Xie
Summary: Sulfation and desulfation of steroids are opposing processes that regulate steroid homeostasis and are involved in steroid-related diseases. Cytosolic sulfotransferase and steroid sulfatase modify and regulate steroids to affect hormonal dysregulation, metabolic diseases, and cancer.
Article
Biochemistry & Molecular Biology
Noah K. Babel, Brian J. Feldman
Summary: Our research identified a previously unrecognized ADAMTS1-PTN-Wnt pathway in regulating adipose precursor cell behavior. The non-muscle myosin protein MYH9 was found to be a key target of this pathway for modulating adipogenesis. These findings offer potential for developing novel therapeutics for obesity and related metabolic disorders.
Article
Biochemistry & Molecular Biology
Martiniano Bello
Summary: This study explores the structural and thermodynamic basis of molecular recognition between ERβ and DPN and its derivatives using molecular dynamics simulations and end-point methods. The results show that the MMGBSA approach is able to reproduce experimental trends and identify eight residues that strongly interact with the ligands.