Bottom-Up Engineering of Well-Defined 3D Microtissues Using Microplatforms and Biomedical Applications

Three-dimensional brain-on-a-chip with an interstitial level of flow and its application as an in vitro model of Alzheimer's disease

(2015) JiSoo Park et al.   LAB ON A CHIP

Immune-protected xenogeneic bioartificial livers with liver-specific microarchitecture and hydrogel-encapsulated cells

(2014) Da Yoon No et al.   BIOMATERIALS

Microchip-based engineering of super-pancreatic islets supported by adipose-derived stem cells

(2014) Yesl Jun et al.   BIOMATERIALS

Optimizing human embryonic stem cells differentiation efficiency by screening size-tunable homogenous embryoid bodies

(2014) Sung-Hwan Moon et al.   BIOMATERIALS

An injectable spheroid system with genetic modification for cell transplantation therapy

(2014) Satoshi Uchida et al.   BIOMATERIALS

A microfluidic-based platform for tumour spheroid culture, monitoring and drug screening

(2014) K. Kwapiszewska et al.   LAB ON A CHIP

A microfluidic chip with a U-shaped microstructure array for multicellular spheroid formation, culturing and analysis

(2014) Chien-Yu Fu et al.   Biofabrication

Engineered Culture Models for Studies of Tumor-Microenvironment Interactions

(2013) David W. Infanger et al.   Annual Review of Biomedical Engineering

Microfluidics-generated pancreatic islet microfibers for enhanced immunoprotection

(2013) Yesl Jun et al.   BIOMATERIALS

Size-controllable networked neurospheres as a 3D neuronal tissue model for Alzheimer's disease studies

(2013) Yoon Jung Choi et al.   BIOMATERIALS

3D co-culturing model of primary pancreatic islets and hepatocytes in hybrid spheroid to overcome pancreatic cell shortage

(2013) Yesl Jun et al.   BIOMATERIALS

Microfluidic fabrication of cell adhesive chitosan microtubes

(2013) Jonghyun Oh et al.   BIOMEDICAL MICRODEVICES

Three-dimensionally engineered biomimetic tissue models forin vitrodrug evaluation: delivery, efficacy and toxicity

(2013) Yvonne Peck et al.   Expert Opinion on Drug Delivery

Spheroid-based three-dimensional liver-on-a-chip to investigate hepatocyte–hepatic stellate cell interactions and flow effects

(2013) Seung-A Lee et al.   LAB ON A CHIP

Metre-long cell-laden microfibres exhibit tissue morphologies and functions

(2013) Hiroaki Onoe et al.   NATURE MATERIALS

Application of Concave Microwells to Pancreatic Tumor Spheroids Enabling Anticancer Drug Evaluation in a Clinically Relevant Drug Resistance Model

(2013) Sang-Eun Yeon et al.   PLoS One

Alginate microcapsule as a 3D platform for the efficient differentiation of human embryonic stem cells to dopamine neurons

(2013) Jaemin Kim et al.   Stem Cell Research

Optimal Formation of Genetically Modified and Functional Pancreatic Islet Spheroids by Using Hanging-Drop Strategy

(2013) H.J. Kim et al.   TRANSPLANTATION PROCEEDINGS

Horseradish peroxidase/catalase-mediated cell-laden alginate-based hydrogel tube production in two-phase coaxial flow of aqueous solutions for filament-like tissues fabrication

(2013) Shinji Sakai et al.   Biofabrication

Size-Dependent Localization and Penetration of Ultrasmall Gold Nanoparticles in Cancer Cells, Multicellular Spheroids, and Tumors in Vivo

(2012) Keyang Huang et al.   ACS Nano

Microfabricated Biomaterials for Engineering 3D Tissues

(2012) Pinar Zorlutuna et al.   ADVANCED MATERIALS

Multi-cell type human liver microtissues for hepatotoxicity testing

(2012) S. Messner et al.   ARCHIVES OF TOXICOLOGY

Recreating the tumor microenvironment in a bilayer, hyaluronic acid hydrogel construct for the growth of prostate cancer spheroids

(2012) Xian Xu et al.   BIOMATERIALS

An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids

(2012) Takahisa Anada et al.   BIOMATERIALS

3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing

(2012) Kazuo Takayama et al.   BIOMATERIALS

Gravity-oriented microfluidic device for uniform and massive cell spheroid formation

(2012) Kangsun Lee et al.   Biomicrofluidics

On-chip three-dimensional tumor spheroid formation and pump-less perfusion culture using gravity-driven cell aggregation and balanced droplet dispensing

(2012) Taeyoon Kim et al.   Biomicrofluidics

On-chip anticancer drug test of regular tumor spheroids formed in microwells by a distributive microchannel network

(2012) Choong Kim et al.   LAB ON A CHIP

Optimization of pancreatic islet spheroid using various concave patterned-films

(2012) Jin Wook Hwang et al.   MACROMOLECULAR RESEARCH

A Heterogeneous In Vitro Three Dimensional Model of Tumour-Stroma Interactions Regulating Sprouting Angiogenesis

(2012) Pedro Correa de Sampaio et al.   PLoS One

Functional 3D Human Primary Hepatocyte Spheroids Made by Co-Culturing Hepatocytes from Partial Hepatectomy Specimens and Human Adipose-Derived Stem Cells

(2012) Da Yoon No et al.   PLoS One

Chondrocyte spheroids on microfabricated PEG hydrogel surface and their noninvasive functional monitoring

(2012) Hidenori Otsuka et al.   SCIENCE AND TECHNOLOGY OF ADVANCED MATERIALS

Stable hydrodynamic trapping of hydrogel beads for on-chip differentiation analysis of encapsulated stem cells

(2012) Choong Kim et al.   SENSORS AND ACTUATORS B-CHEMICAL

Development of a three-dimensional microfluidic system for long-term tumor spheroid culture

(2012) Karina Ziółkowska et al.   SENSORS AND ACTUATORS B-CHEMICAL

Microfluidic synthesis of chemically and physically anisotropic hydrogel microfibers for guided cell growth and networking

(2012) Masumi Yamada et al.   Soft Matter

Surface Tension-Mediated, Concave-Microwell Arrays for Large-Scale, Simultaneous Production of Homogeneously Sized Embryoid Bodies

(2012) Gi Seok Jeong et al.   Advanced Healthcare Materials

Three-dimensional cell culture based on microfluidic techniques to mimic living tissues

(2012) Yuya Morimoto et al.   Biomaterials Science

Molding Cell Beads for Rapid Construction of Macroscopic 3D Tissue Architecture

(2011) Yukiko T. Matsunaga et al.   ADVANCED MATERIALS

Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model

(2011) Sau Fung Wong et al.   BIOMATERIALS

A cell-instructive hydrogel to regulate malignancy of 3D tumor spheroids with matrix rigidity

(2011) Youyun Liang et al.   BIOMATERIALS

In situ formation and collagen-alginate composite encapsulation of pancreatic islet spheroids

(2011) Bo Ram Lee et al.   BIOMATERIALS

Micro-ring structures stabilize microdroplets to enable long term spheroid culture in 384 hanging drop array plates

(2011) Amy Y. Hsiao et al.   BIOMEDICAL MICRODEVICES

384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids

(2011) Amy Y. Hsiao et al.   BIOTECHNOLOGY AND BIOENGINEERING

Bottom-up tissue engineering

(2011) Donald L Elbert   CURRENT OPINION IN BIOTECHNOLOGY

Optimal size control of pancreatic islet cell clusters using concave-bottomed microwells

(2011) Jin Wook Hwang et al.   JOURNAL OF CONTROLLED RELEASE

3-Dimensional cell culture for on-chip differentiation of stem cells in embryoid body

(2011) Choong Kim et al.   LAB ON A CHIP

Optimised production of multifunctional microfibres by microfluidic chip technology for tissue engineering applications

(2011) Stefania Mazzitelli et al.   LAB ON A CHIP

Meniscus induced self organization of multiple deep concave wells in a microchannel for embryoid bodies generation

(2011) Gi Seok Jeong et al.   LAB ON A CHIP

Microfluidic fabrication of microengineered hydrogels and their application in tissue engineering

(2011) Bong Geun Chung et al.   LAB ON A CHIP

Digitally tunable physicochemical coding of material composition and topography in continuous microfibres

(2011) Edward Kang et al.   NATURE MATERIALS

Killing Hypoxic Cell Populations in a 3D Tumor Model with EtNBS-PDT

(2011) Conor L. Evans et al.   PLoS One

Micro-Masonry: Construction of 3D Structures by Microscale Self-Assembly

(2010) Javier G. Fernandez et al.   ADVANCED MATERIALS

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array

(2010) Yi-Chung Tung et al.   ANALYST

A neurospheroid network-stamping method for neural transplantation to the brain

(2010) Midori Kato-Negishi et al.   BIOMATERIALS

Controlled-size embryoid body formation in concave microwell arrays

(2010) Yoon Young Choi et al.   BIOMATERIALS

Synergistic Enhancement of Carboplatin Efficacy with Photodynamic Therapy in a Three-Dimensional Model for Micrometastatic Ovarian Cancer

(2010) I. Rizvi et al.   CANCER RESEARCH

Modelling the hair follicle dermal papilla using spheroid cell cultures

(2010) Claire A. Higgins et al.   EXPERIMENTAL DERMATOLOGY

Effect of microwell chip structure on cell microsphere production of various animal cells

(2010) Yusuke Sakai et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Preparation of arrays of cell spheroids and spheroid-monolayer cocultures within a microfluidic device

(2010) Tomoaki Okuyama et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

A Review of Three-Dimensional In Vitro Tissue Models for Drug Discovery and Transport Studies

(2010) Nelita T. Elliott et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval

(2010) Edward Kang et al.   LAB ON A CHIP

Microfluidic synthesis of pure chitosan microfibers for bio-artificial liver chip

(2010) Kwang Ho Lee et al.   LAB ON A CHIP

Generation of core-shell microcapsules with three-dimensional focusing device for efficient formation of cell spheroid

(2010) Choong Kim et al.   LAB ON A CHIP

A multicellular spheroid formation and extraction chip using removable cell trapping barriers

(2010) Hye-Jin Jin et al.   LAB ON A CHIP

Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their antiinflammatory properties

(2010) T. J. Bartosh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interface-Directed Self-Assembly of Cell-Laden Microgels

(2010) Behnam Zamanian et al.   Small

Autocrine-Controlled Formation and Function of Tissue-Like Aggregates by Primary Hepatocytes in Micropatterned Hydrogel Arrays

(2010) Courtney M. Williams et al.   TISSUE ENGINEERING PART A

Tissue engineering by self-assembly and bio-printing of living cells

(2010) Karoly Jakab et al.   Biofabrication

Mass Fabrication of Small Cell Spheroids by Using Micro-patterned Tissue Culture Plate

(2009) Akinari Iwasaki et al.   ADVANCED ENGINEERING MATERIALS

Anti-tumor activity of noble indirubin derivatives in human solid tumor models In Vitro

(2009) Soo-Hyun Kim et al.   ARCHIVES OF PHARMACAL RESEARCH

Novel technique to control inner and outer diameter of calcium-alginate hydrogel hollow microfibers, and immobilization of mammalian cells

(2009) Takayuki Takei et al.   BIOCHEMICAL ENGINEERING JOURNAL

3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells

(2009) Wenjie Wang et al.   BIOMATERIALS

Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids

(2009) Amy Y. Hsiao et al.   BIOMATERIALS

Controlled cellular orientation on PLGA microfibers with defined diameters

(2009) C. M. Hwang et al.   BIOMEDICAL MICRODEVICES

Comparative Analysis of Gene Expression in Rat Liver Tissue and Monolayer- and Spheroid-Cultured Hepatocytes

(2009) Yusuke Sakai et al.   CELLS TISSUES ORGANS

Penetration and efficacy of VEGF siRNA using polyelectrolyte complex micelles in a human solid tumor model in-vitro

(2009) Ahmed M. Al-Abd et al.   JOURNAL OF CONTROLLED RELEASE

Inverted pattern formation of cell microarrays on poly(ethylene glycol) (PEG) gel patterned surface and construction of hepatocyte spheroids on unmodified PEG gel microdomains

(2009) Keitaro Yoshimoto et al.   LAB ON A CHIP

Rapid exchange of oil-phase in microencapsulation chip to enhance cell viability

(2009) Choong Kim et al.   LAB ON A CHIP

Study of cellular behaviors on concave and convex microstructures fabricated from elastic PDMS membranes

(2009) Joong Yull Park et al.   LAB ON A CHIP

Three-dimensional spheroid-forming lab-on-a-chip using micro-rotational flow

(2009) Hiroki Ota et al.   SENSORS AND ACTUATORS B-CHEMICAL

Synthesis of Cell-Laden Alginate Hollow Fibers Using Microfluidic Chips and Microvascularized Tissue-Engineering Applications

(2009) Kwang Ho Lee et al.   Small

Modular tissue engineering: engineering biological tissues from the bottom up

(2009) Jason W. Nichol et al.   Soft Matter

Differentiation of Neural Progenitor Cells in a Microfluidic Chip-Generated Cytokine Gradient

(2009) Joong Yull Park et al.   STEM CELLS

Recent advances in three-dimensional multicellular spheroid culture for biomedical research

(2008) Ruei-Zhen Lin et al.   Biotechnology Journal

Differentiation of Embryonic Stem Cells to Clinically Relevant Populations: Lessons from Embryonic Development

(2008) Charles E. Murry et al.   CELL

Method of Bottom-Up Directed Assembly of Cell-Laden Microgels

(2008) Yanan Du et al.   Cellular and Molecular Bioengineering

VEGF release by MMP-9 mediated heparan sulphate cleavage induces colorectal cancer angiogenesis

(2008) Lukas J.A.C. Hawinkels et al.   EUROPEAN JOURNAL OF CANCER

Micropatterned organoid culture of rat hepatocytes and HepG2 cells

(2008) Ryuhei Mori et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

From single cells to tissue architecture—a bottom-up approach to modelling the spatio-temporal organisation of complex multi-cellular systems

(2008) J. Galle et al.   JOURNAL OF MATHEMATICAL BIOLOGY

Tubular gel fabrication and cell encapsulation in laminar flow stream formed by microfabricated nozzle array

(2008) Shinji Sugiura et al.   LAB ON A CHIP

Fabrication of a modular tissue construct in a microfluidic chip

(2008) Derek A. Bruzewicz et al.   LAB ON A CHIP

Microfluidic Chip-Based Fabrication of PLGA Microfiber Scaffolds for Tissue Engineering

(2008) Chang Mo Hwang et al.   LANGMUIR

Adenovirus-mediated transfer of siRNA against MMP-2 mRNA results in impaired invasion and tumor-induced angiogenesis, induces apoptosis in vitro and inhibits tumor growth in vivo in glioblastoma

(2008) O Kargiotis et al.   ONCOGENE

Tissue Engineering by Self-Assembly of Cells Printed into Topologically Defined Structures

(2008) Karoly Jakab et al.   TISSUE ENGINEERING PART A

A microwell array system for stem cell culture

(2007) Hannes-Christian Moeller et al.   BIOMATERIALS

Development of an in Vitro Multicellular Tumor Spheroid Model Using Microencapsulation and Its Application in Anticancer Drug Screening and Testing

(2005) Xulang Zhang et al.   BIOTECHNOLOGY PROGRESS