Article
Engineering, Biomedical
Stasia Krishtul, Michal Skitel Moshe, Inna Kovrigina, Limor Baruch, Marcelle Machluf
Summary: In this study, a microencapsulation platform based on porcine pancreatic extracellular matrix (pECM) was developed to enhance the performance of pancreatic islet cells. pECM-encapsulated islet cells exhibited higher insulin secretion levels in vitro, as well as proliferation and production of an enriched intra-islet ECM framework. pECM encapsulation also provided temporary protection against cytokine-induced cytotoxicity. In vivo experiments showed that this platform significantly improved glucose tolerance and achieved glycemic correction in immunocompetent diabetic mice without immunosuppression.
ACTA BIOMATERIALIA
(2023)
Review
Endocrinology & Metabolism
Naomi Koehler, Leo Buhler, Bernhard Egger, Carmen Gonelle-Gispert
Summary: This review summarizes the immunomodulatory and anti-inflammatory properties of mesenchymal stromal cells (MSC) in the context of islet transplantation, highlighting the potential of co-culturing and co-transplanting islet cells with MSC to enhance islet proliferation and maturation, insulin secretion, and graft survival.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Medicine, Research & Experimental
Amoge Opara, Alec Jost, Sam Dagogo-Jack, Emmanuel C. Opara
Summary: This minireview outlines the characteristics of diabetes, the potential of encapsulated pancreatic islet transplantation for type 1 diabetes, the pros and cons of different encapsulation methods, as well as the challenges and emerging strategies in clinical translation.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Patricio Atanes, Tanyel Ashik, Shanta J. Persaud
Summary: This study compared the expression of GPCR mRNA in human islets obtained from donors with normal weight range and those classified as obese. The altered expression of GPCRs in obesity may have an impact on islet function, and the study also considered the possible effects of adipokines on GPCRs showing altered expression in obesity.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Medicine, Research & Experimental
Jessica D. Kepple, Jessie M. Barra, Martin E. Young, Chad S. Hunter, Hubert M. Tse
Summary: Transplanting islets into brown adipose tissue (BAT) may be a viable alternative transplant site that improves graft survival and delays immune rejection.
Article
Medicine, Research & Experimental
Robert W. Holdcraft, Melanie J. Graham, Melissa A. Bemrose, Lucas A. Mutch, Prithy C. Martis, Jody L. Janecek, Richard D. Hall, Barry H. Smith, Lawrence S. Gazda
Summary: Human islet transplantation has limitations in supply, leading to the proposal of porcine islets as an alternative. This study demonstrates the safety and effectiveness of encapsulated porcine islets in reducing insulin requirements and supports its potential as a treatment option for diabetes mellitus.
XENOTRANSPLANTATION
(2022)
Article
Biochemistry & Molecular Biology
Wei Wang, Yahao Wang, Jingwei Chi, Xiaojun Tan, Jianxia Hu, Xiaolong Ma, Xiaofang Sun, Kui Che, Wenshan Lv, Yangang Wang
Summary: The study demonstrated that human umbilical cord mesenchymal stem cells carrying exenatide could improve blood glucose levels and body weight, reduce immune cell infiltration and islet tissue changes in type 1 diabetes mellitus mice. Moreover, treatment with exenatide-carrying hUCMSCs also attenuated renal tissue lesions in T1DM mice. Additionally, the beneficial effects were suggested to be related to changes in intestinal bacteria composition.
MOLECULAR MEDICINE
(2022)
Article
Endocrinology & Metabolism
Aaron L. Glieberman, Benjamin D. Pope, Douglas A. Melton, Kevin Kit Parker
Summary: Diabetes is a disease of insulin insufficiency, where islet transplantation offers a potential therapy, but is still limited by the availability of human islets and reliable function testing. Stem cell technologies may fill the shortage of transplantable cells, yet better methods are needed to predict transplantation outcomes.
Article
Nanoscience & Nanotechnology
Sang Hoon Lee, Hyun-Ouk Kim, Jung-Taek Kang
Summary: Research focuses on nano-encapsulation of neonatal porcine islet-like cell clusters using PSomes. NHS is utilized to bind PSomes to cells, and F-10 culture medium with BSA is used to minimize damage and loss of cells during encapsulation. Efficient encapsulation is achieved through cross-linking bifunctional PSomes.
NANOSCALE RESEARCH LETTERS
(2021)
Article
Cell & Tissue Engineering
Rei Kuwabara, Shuxian Hu, Alexandra M. Smink, Gorka Orive, Jonathan R. T. Lakey, Paul de Vos
Summary: Islet transplantation holds promise for treating insulin-dependent diabetes, but challenges remain due to the lack of a consistent source of insulin-producing cells and the need for lifelong administration of immunosuppressive drugs. Immunoisolation using polymeric membranes shows safety and feasibility in controlling glucose homeostasis, but long-term graft survival is not yet achieved. Combining immunoisolation with immunomodulation strategies may enhance the longevity of islet grafts, but further research is needed to fully understand the reasons for final graft failure and improve outcomes.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Endocrinology & Metabolism
George P. Brownrigg, Yi Han Xia, Chieh Min Jamie Chu, Su Wang, Charlotte Chao, Jiashuo Aaron Zhang, Sos Skovso, Evgeniy Panzhinskiy, Xiaoke Hu, James D. Johnson, Elizabeth J. Rideout
Summary: This study aims to investigate the crucial role of pancreatic beta cells in maintaining glucose homeostasis and the impact of sex differences on this process. By comparing the gene expression and function of beta cells in males and females, we found that female patients have more reliable insulin secretion and higher resilience to endoplasmic reticulum stress.
MOLECULAR METABOLISM
(2023)
Article
Chemistry, Physical
Qun Huang, Yuting Cai, Xinrui Yang, Weimin Li, Hongji Pu, Zhenjing Liu, Hongwei Liu, Mohsen Tamtaji, Feng Xu, Liyuan Sheng, Tae-Hyung Kim, Shiqing Zhao, Dazhi Sun, Jinbao Qin, Zhengtang Luo, Xinwu Lu
Summary: ADSCs show potential for treating DPNI by enhancing proliferation and migration of Schwann cells and endothelial cells. A novel GF/hydrogel scaffold delivers ADSCs effectively, accelerating Schwann cell proliferation and promoting DPNI recovery.
Article
Cell & Tissue Engineering
Rahul Khatri, Sebastian Friedrich Petry, Thomas Linn
Summary: The study suggests that the physical contact between MSC and pancreatic islet cells is necessary for unleashing their protective potential fully. Administration of MSC through the intrapancreatic route improved hyperglycemia, promoted proliferation of islet cells, increased islet number and area, enhanced expression of growth factor (EGF), and balanced the Th1/Th2 response in vivo. Additionally, direct physical contact provided superior protection to MIN6-cells from STZ damage in vitro through the AKT and ERK pathways.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Endocrinology & Metabolism
Eckhard Wolf, Elisabeth Kemter, Lelia Wolf-van Buerck, Jochen Seissler
Summary: Islet cell transplantation is the best therapeutic option for type 1 diabetes patients who experience severe hypoglycaemia despite intensive insulin therapy. However, there is a shortage of human donors, leading to ongoing research on xenotransplantation using animal pancreatic islets, with pigs being the preferred donor. Recent advancements in encapsulation strategies, immunosuppressive protocols, and genetic modification of donor pigs have greatly improved the efficacy of xenogeneic islet cell transplantation in diabetic non-human primate models. This progress brings promise for future clinical trials.
Article
Pharmacology & Pharmacy
Winifred P. S. Wong, Janice C. Wang, Matthew J. Schipma, Xiaomin Zhang, Joshua R. Edwards, Malek El Muayed
Summary: Cadmium exposure is associated with changes in gene expression and molecular regulatory networks in pancreatic islets, contributing to the development of Type II diabetes mellitus. Cadmium affects intracellular protein trafficking to the plasma membrane, impacting genes involved in glucose homeostasis.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Se Yong Jung, Min Seo Kim, Han Li, Keum Hwa Lee, Ai Koyanagi, Marco Solmi, Andreas Kronbichler, Elena Dragioti, Kalthoum Tizaoui, Sarah Cargnin, Salvatore Terrazzino, Sung Hwi Hong, Ramy Abou Ghayda, Nam Kyun Kim, Seo Kyoung Chung, Louis Jacob, Joe-Elie Salem, Dong Keon Yon, Seung Won Lee, Karel Kostev, Ah Young Kim, Jo Won Jung, Jae Young Choi, Jin Soo Shin, Soon-Jung Park, Seong Woo Choi, Kiwon Ban, Sung-Hwan Moon, Yun Young Go, Jae Il Shin, Lee Smith
Summary: The study utilized a database of cardiovascular adverse drug reactions and in vitro experiments to find that remdesivir is associated with cardiac arrest, bradycardia, and hypotension, highlighting the importance of adequate cardiovascular monitoring post remdesivir administration.
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Hyung Shik Kim, Minwook Seo, Tae-Eun Park, Dong Yun Lee
Summary: In this study, nanoconjugates composed of gold nanoparticles and photosensitizers were prepared. These nanoconjugates exhibit high heat dissipation and ROS generating capability. By incorporating lactoferrin onto the surface of the nanoconjugates, targeted therapy for GBM can be achieved, and the oral administration improves the bioavailability.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Hyung Shik Kim, Seung Jae Lee, Dong Yun Lee
Summary: Orally absorbable gold nanoparticles conjugated with milk protein lactoferrin target glioblastoma multiforme (GBM) through interaction with lactoferrin receptors. They demonstrate significant hyperthermia effects and reduce tumor volume effectively.
BIOACTIVE MATERIALS
(2022)
Article
Oncology
Soon-Jung Park, Hyeok Kim, Sunghun Lee, Jongsoo Kim, Taek-Hee Jung, Seong Woo Choi, Bong-Woo Park, Sun-Woong Kang, David A. Elliott, Edouard G. Stanley, Andrew G. Elefanty, Kiwon Ban, Hun-Jun Park, Sung-Hwan Moon
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Hyung Shik Kim, Dong Yun Lee
Summary: Current treatment options for malignant brain tumors are limited in their effectiveness, with a low survival rate for patients with highly malignant glioblastoma multiforme (GBM). Challenges in treating GBM include the complexity of brain anatomy, the blood-brain barrier preventing drugs from reaching the tumor, and the heterogeneity of GBM tissues at the cellular level. Photodynamic therapy (PDT) offers a potential solution by selectively targeting tumor cells while protecting healthy brain regions, and nanomedicine advancements can further improve the clinical outcomes of PDT-treated GBM.
Article
Engineering, Biomedical
Ju-Chan Park, Jumee Kim, Hyun-Ki Jang, Seung-Yeon Lee, Keun-Tae Kim, Eun-Ji Kwon, Seokwoo Park, Hyun Sik Lee, Hyewon Choi, Seung-Yeol Park, Hee-Jung Choi, Soon-Jung Park, Sung-Hwan Moon, Sangsu Bae, Hyuk-Jin Cha
Summary: This study successfully established multiple isogenic disease models of GNE myopathy by using base editors to construct hPSCs. Different mutations in different domains of the GNE gene were found to cause mutation-specific hyposialylation and gene expression changes, which were closely related to pathological clinical phenotypes. Furthermore, treatment with a drug candidate currently under clinical trials showed a mutation-specific drug response in GNE myopathy disease models.
Article
Multidisciplinary Sciences
Hyosuk Kim, Byeong-Wook Song, Soon-Jung Park, Seong Woo Choi, Hanbyeol Moon, Ki-Chul Hwang, Sun-Woong Kang, Sung-Hwan Moon, Yoosoo Yang, Ick Chan Kwon, Sun Hwa Kim
Summary: This study proposes an extracellular vesicle (EV)-guided, nonviral, direct lineage conversion strategy to enhance the transdifferentiation of fibroblasts to induced cardiomyocyte-like cells (iCMs). The resulting iCMs show typical cardiac features and have gene expression profiles similar to cardiomyocytes. This method achieves a high conversion efficiency (>60%) and establishes a more accessible platform for generating specialized somatic cells through direct lineage conversion.
Article
Cell Biology
Soo-Been Jeon, A-Reum Han, Sunghun Lee, Seung Chan Lee, Min Ji Lee, Soon-Jung Park, Sung-Hwan Moon, Ji Yoon Lee
Summary: In this study, a CD34(dim) subpopulation with hemogenic potential and a purified HE population that generated hematopoietic stem cells and lymphocytes were identified and validated. A simple method to purify HE cells enriched in CD34(dim) cells was developed. The findings revealed an initial step in differentiating hematopoietic lineage cells and its potential application in regenerative medicine.
CELL PROLIFERATION
(2023)
Article
Chemistry, Multidisciplinary
Hae Hyun Hwang, Hyung Shik Kim, Dong Yun Lee
Summary: The conjugate of lactoferrin and heparin can attenuate angiogenic activity by binding to growth factors around the tumor tissue, and it can be orally absorbed and delivered to brain tumors through the blood-brain barrier. It inhibits angiogenesis in glioblastoma and shows potential as a new oral medication for treatment.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Hyung Shik Kim, Sieun Lee, Dong Yun Lee
Summary: Aurozyme, a novel nanomedicine composed of gold nanoparticles and glycyrrhizin, shows promise as a therapeutic strategy for colitis. It converts harmful peroxidase-like activity to beneficial catalase-like activity, effectively scavenging reactive oxygen/reactive nitrogen species and damage-associated molecular patterns. It promotes anti-inflammatory effects, restores intestinal function, and increases beneficial probiotics, highlighting its potential for the treatment of inflammatory diseases.
Article
Multidisciplinary Sciences
Dongkyu Kang, Hyung Shik Kim, Soohyun Han, Yeonju Lee, Young-Pil Kim, Dong Yun Lee, Joonseok Lee
Summary: The authors designed a Nd-Yb co-doped nanomaterial as a near-infrared long-lifetime imaging-guided water-heating probe, demonstrating photothermal ablation in a glioblastoma pre-clinical model.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Hyung Shik Kim, Seok Chan Park, Hae Jin Kim, Dong Yun Lee
Summary: This study develops the Lf-GL conjugate to address the issues of GL's pharmacokinetics and delivery to tumor sites. It demonstrates that Lf-GL efficiently inhibits the action of HMGB1 in the tumor microenvironment, leading to the suppression of tumor angiogenesis and development. Moreover, Lf-GL improves the pharmacokinetic properties of GL and reduces tumor growth in a glioblastoma mouse model.
BIOMATERIALS RESEARCH
(2023)
Review
Medicine, Research & Experimental
Hee-Jae Jeon, Hyung Shik Kim, Euiheon Chung, Dong Yun Lee
Summary: This review discusses the application of nanozyme-based colorimetric detection methods in monitoring and managing diabetes. Nanozymes have advantages such as high sensitivity, stability, and flexible structural design, making them a potential alternative to natural enzymes.
Article
Biochemistry & Molecular Biology
Hyeok Kim, Soon-Jung Park, Jae-Hyun Park, Sunghun Lee, Bong-Woo Park, Soon Min Lee, Ji-Won Hwang, Jin-Ju Kim, Byeongmin Kang, Woo-Sup Sim, Hyo-Jin Kim, Seung Hwan Jeon, Dong-Bin Kim, Jinah Jang, Dong-Woo Cho, Sung-Hwan Moon, Hun-Jun Park, Kiwon Ban
Summary: Researchers have successfully used a combination of two different types of stem cells to repair failed hearts by creating new blood vessels. This dual stem cell treatment improves cardiac function and results in a higher number of larger and stronger new blood vessels.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Materials Science, Biomaterials
Soon-Jung Park, Taek-Hee Jung, Jong Hyun Kim, Kyoung-Yong Lee, Jeongyun Kim, Jongil Ju, Sung-Hwan Moon
Summary: This study evaluated the application of computational fluid dynamics in an in silico-designed and spheroid-based 3D cell culture chip. The results demonstrated the usefulness of computational simulation in cell culture, drug screening, cytokine delivery, and differentiation. The study also showed that low flow rates could facilitate the formation of uniform cell spheroids, and advanced cell culture technologies have the potential to accelerate research and development of cell therapies.
BIOMATERIALS SCIENCE
(2022)
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.
