Article
Chemistry, Multidisciplinary
Reza Rasouli, Maryam Tabrizian
Summary: 3D cell spheroid culture provides a faithful recreation of cell growth environment compared to 2D culture, with a new boundary-driven acoustic microstreaming tool that accelerates spheroid production, showing versatility in producing different types of spheroids.
Article
Nanoscience & Nanotechnology
Bin Chen, Zhuhao Wu, Yue Wu, Yue Chen, Lei Zheng
Summary: Researchers have developed a novel contactless acoustofluidic device for the controllable fusion of multicellular spheroids. The device utilizes standing sound waves to trap and assemble spheroids in a biocompatible, label-free, and contact-free manner. With time-lapse imaging and in situ culture, the system enables manipulation and tracking of dynamic interactions between spheroids. This tool can be valuable for 3D biological self-assembly and translational applications in regenerative medicine.
MICROFLUIDICS AND NANOFLUIDICS
(2023)
Article
Chemistry, Analytical
Arash Yahyazadeh Shourabi, Roozbeh Salajeghe, Maryam Barisam, Navid Kashaninejad
Summary: Microfluidic lab-on-chip devices are widely used for chemical and biological studies, with perfusion microwells for culturing multicellular spheroids being a common type. A novel acoustofluidic integrated platform is proposed to enhance cell viability and reduce necrotic and quiescent zones in cultured spheroids without increasing flow rate, thus decreasing reagent consumption significantly. Numerical simulations show that increasing acoustic boundary displacement amplitude enlarges the proliferating zone of spheroids, and implementing certain parameters can greatly reduce the required flow rate to maintain necrotic zones.
Article
Chemistry, Multidisciplinary
Jin-Ha Choi, Hye Kyu Choi, Ki-Bum Lee
Summary: The human neurovascular system plays a crucial role in brain function and has been of interest for developing drugs to treat neuroinflammation. Animal models have limitations in accurately reflecting human clinical trial outcomes, leading to medication failures. To overcome this, a neurovascular-unit-on-a-chip system is developed to replicate the human neurovascular microenvironment, providing a more accurate representation of human physiology. The ability to detect proinflammatory cytokines and monitor physiological changes can greatly aid in drug evaluation. Using a nano-biosensing approach, the neuroinflammation-on-a-chip system has the potential to revolutionize neuroinflammation research and drug development.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Multidisciplinary Sciences
Karl Olofsson, Valentina Carannante, Madoka Takai, Bjorn onfelt, Martin Wiklund
Summary: Ultrasound-based MCTS culture platform was utilized to connect nuclear segmentation to biological information in A498 renal carcinoma MCTSs, showing potential for cell cycle state classification and nuclear volumetric characterization.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Chemical
JunHwee Jang, Eun-Jung Lee
Summary: Cell spheroids have been studied as a biomimetic medicine for tissue healing, with rapid production using MXene particles, inducing spheroid formation within 6 hours at concentrations of 1 to 10 mu g/mL. Osteogenic differentiation behavior was observed with the highest activity levels at a concentration of 5 mu g/mL, showing an effective method for rapid formation of stem cell spheroids using MXene.
Article
Biochemical Research Methods
Alina Peethan, M. Aravind, Santhosh Chidangil, Sajan D. George
Summary: Droplet splitting using tailored surface wettability is an important technique for creating ultralow volumes of samples, which have various applications in bioassays, tissue engineering, and material synthesis. In this work, a non-adhesive contrast quartz substrate is fabricated for the trapping and analysis of single biological cells using Raman spectroscopy. The substrate allows the splitting of microliter droplets into smaller daughter droplets, enabling high throughput screening and analysis of single cells.
Article
Oncology
Stephanie Le Compton, Emily S. Pyne, Lu Liu, Jack Guinan, Amanda A. Shea, Joseph P. Grieco, Madlyn Frisard, Eva M. Schmelz
Summary: The study found that ovarian cancer cells acquire a more glycolytic and metabolically flexible phenotype during progression. Hypoxia and aggregation conditions result in reduced cellular metabolism, proliferation, and drug response. Recruitment of obese stromal vascular fraction (SVF) can enhance invasive capacity in aggressive cells but reduces respiration.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sergei V. German, Anatolii A. Abalymov, Maxim A. Kurochkin, Yuliya Kan, Dmitry A. Gorin, Marina V. Novoselova
Summary: In order to enhance the study of drugs and contrast agents, 3D microfluidic platforms are being actively developed for in vitro testing. A microfluidic lymph node-on-chip (LNOC) was created as a tissue engineered model of a secondary tumor in the lymph node. The developed chip demonstrated suitability for pharmacological applications and showed that smaller size capsules had better penetration into the tumor spheroid. This device could potentially replace in vivo models for early secondary tumors and reduce the need for in vivo experiments in preclinical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Rachel Ringquist, Delta Ghoshal, Ritika Jain, Krishnendu Roy
Summary: The tumor microenvironment (TME) is shaped by dynamic interactions between various cell types, influencing immune responses and therapeutic resistance. Efforts are being made to understand key components and model complexity for studying immunotherapies. Challenges in sourcing and quality control of TME cells persist in developing reproducible platforms for cell therapies.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Engineering, Biomedical
Xin Lei, Changmin Shao, Xin Shou, Keqing Shi, Liang Shi, Yuanjin Zhao
Summary: This paper introduced a method using porous hydrogel arrays to study drug resistance in hepatoma cells and found that the arrays have great potential in forming cell spheroids and simulating drug resistance.
BIO-DESIGN AND MANUFACTURING
(2021)
Article
Engineering, Biomedical
Shun Li, Kaifu Yang, Xiangyan Chen, Xinglong Zhu, Hanying Zhou, Ping Li, Yu Chen, Ying Jiang, Tingting Li, Xiang Qin, Hong Yang, Chunhui Wu, Bao Ji, Fengming You, Yiyao Liu
Summary: Cell culture systems play a crucial role in biomedical research. 3D cell culture technology is gaining attention for its unique biochemical and biophysical properties compared to traditional 2D cell cultures, especially in cancer and stem cell research.
Article
Biochemistry & Molecular Biology
Bo Seul Jang, Kyoung Hwan Park, Eun Yeong Suh, Byoung-Seok Lee, Sun-Woong Kang, Kang Moo Huh
Summary: Chemically crosslinked hydrogels prepared by photocrosslinking of methacrylated HGCs demonstrated enhanced gel stability and good spheroid-forming abilities for long-term 3D cell cultures. M-HGC-coated dishes effectively generated spheroids of larger size and higher cell density depending on the crosslinking density of the M-HGCs, showing potential for widely applied in tissue engineering and drug screening. The hydrogel-coated dish system provides an effective technique to produce cell spheroids with customized sizes and densities essential for various applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Analytical
Yanjuan Wang, Ning Tong, Fengqi Li, Kai Zhao, Deguang Wang, Yijie Niu, Fengqiang Xu, Jiale Cheng, Junsheng Wang
Summary: In this study, a microfluidic trapping system based on AC dielectrophoresis (DEP) technology was proposed for precise trapping and release of specific microparticles. Numerical simulation and experimental verification were conducted to confirm the effectiveness of the designed system, contributing to the application of cell trapping and manipulation, as well as single-cell analysis.
Article
Cell Biology
Vivek Kumar, Mohit Vashishta, Lin Kong, Jiade J. Lu, Xiaodong Wu, Bilikere S. Dwarakanath, Chandan Guha
Summary: Photon-based radiotherapy and carbon ion radiotherapy have different effects on Notch signaling in glioma cells. Carbon ion radiotherapy regulates the expression of Notch signaling and reduces stemness and cell migration. These findings may contribute to reducing tumor regrowth and disease dissemination.