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Biochemistry & Molecular Biology
Eva Laura von der Heyde, Armin Hallmann
Summary: The study of cell division in green algae reveals the dynamics of subcellular structures and provides insight into the evolution of cell division.
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Biochemistry & Molecular Biology
Jiyeon Leem, Jeong Su Oh
Summary: In this study, researchers discovered that MDC1 plays a non-canonical role in controlling G2/M transition in mouse oocytes by regulating APC/C-Cdh1-mediated cyclin B1 degradation in response to DNA damage. They also found that MDC1 depletion impairs spindle assembly by decreasing the integrity of microtubule organizing centers (MTOCs). These findings provide new insights into the regulation of the G2/M DNA damage checkpoint and cell cycle control in oocytes.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
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Plant Sciences
Nathaniel Perry, Colin D. Leasure, Hongyun Tong, Elias M. Duarte, Zheng-Hui He
Summary: The Arabidopsis RUS gene family contains six members, with RUS6 being essential for early embryo development. The RUS gene family is involved in multiple stages of plant development.
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Environmental Sciences
Yaming Ge, Fei Ren, Lingli Chen, Dongfang Hu, Xinrui Wang, Yunli Cui, Yu Suo, Hongli Zhang, Junping He, Zhihong Yin, Hongmei Ning
Summary: The study revealed that BPA exerts toxic effects on the development and behavior of Xenopus embryos, inducing DNA damage, apoptosis, and multiple malformations.
ENVIRONMENTAL POLLUTION
(2021)
Article
Multidisciplinary Sciences
Kevin D. Whitley, Calum Jukes, Nicholas Tregidgo, Eleni Karinou, Pedro Almada, Yann Cesbron, Ricardo Henriques, Cees Dekker, Seamus Holden
Summary: The study reveals the critical functions of FtsZ treadmilling in bacterial cell division, including condensing FtsZ filaments and guiding cell wall synthesis, essential for assembling and initiating the bacterial cell division machine.
NATURE COMMUNICATIONS
(2021)
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Reproductive Biology
Qing Zhang, Wenjing Li, Pei Feng, Yayi Liu, Peng Meng, Bo Chu, Jianglin Zhao, Yanxue Li, Yong Zhang, Jun Liu
Summary: This study investigates the role of lncRNA lnc5926 in early embryonic development in goats. The expression of lnc5926 is highest at the eight-cell embryonic stage, consistent with the pattern of embryonic genome activation (EGA) gene expression. Knockdown of lnc5926 leads to a significant decrease in the blastocyst rate and downregulation of key EGA genes, while overexpression of lnc5926 reverses these effects. The findings suggest that lnc5926 plays an essential role in goat embryonic development by regulating EGA-associated gene expression.
Article
Biology
Lev Brio, Danit Wasserman, Efrat Michaely-Barbiro, Gal Barazany-Gal, Doron Gerber, Amit Tzur
Summary: Protein degradation mediated by the ubiquitin-proteasome pathway plays a crucial role in regulating signaling events. In vitro degradation assays, although laborious, have been important in understanding cell proliferation and other cellular processes. We present a microfluidic technology called protein degradation on chip (pDOC) that enables quick and simultaneous protein degradation assays using minute amounts of reagents. This technology offers a sensitive and high-throughput alternative to conventional degradation assays.
COMMUNICATIONS BIOLOGY
(2022)
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Cell Biology
Qiang Fang, Xue-Lin Chen, Lei Zhang, Ya-Bin Li, Tian-Zeng Sun, Chen-Xin Yang, Jian-Feng Chang, Xiao-Mei Yang, Feng Sun
Summary: MPS1 is crucial for spermatogenesis, and its absence may lead to cell loss and meiosis blockage during spermatogenesis. MPS1 plays a key role in the formation of sperms and fertility.
CELL DEATH & DISEASE
(2021)
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Obstetrics & Gynecology
Li Li, Ye Xia, Yang Yang, Wuwen Zhang, Hua Yan, Ping Yin, Kai Li, Yuanyuan Chen, Lu Lu, Guoqing Tong
Summary: CDC26 plays a key role in human oocyte aging, as its lack can disrupt the maturation process and lead to oocyte aging. The study suggests that overexpression of CDC26 can partially rescue the defects in aged oocytes.
HUMAN REPRODUCTION
(2021)
Article
Multidisciplinary Sciences
Christopher Thomas, Benjamin Wetherall, Mark D. Levasseur, Rebecca J. Harris, Scott T. Kerridge, Jonathan M. G. Higgins, Owen R. Davies, Suzanne Madgwick
Summary: The study reveals an excess of securin over separase in mouse oocytes during meiosis I, with a mechanism promoting securin destruction in prometaphase I. This destruction mechanism relies on specific residues within securin that are exposed when not bound to separase. The authors suggest that this mechanism is crucial for successful meiotic progression in mouse oocytes by ensuring the removal of non-separase-bound securin before metaphase.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Iga Dalidowska, Olga Gazi, Dorota Sulejczak, Maciej Przybylski, Pawel Bieganowski
Summary: The research showed that the Hsp90 inhibitor 17-AAG effectively reduces the replication rate of human adenovirus 5 and inhibits its transcription of early and late genes, viral DNA replication, and protein expression. Additionally, 17-AAG decreases the level of newly synthesized E1A protein, potentially explaining its anti-adenoviral activity by reducing E1A levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Marine & Freshwater Biology
Hannah Flach, Alexander Lenz, Sarah Pfeffer, Michael Kuhl, Susanne J. Kuhl
Summary: This study demonstrates that the herbicide glyphosate-based herbicide (GBH) negatively affects the embryonic development of the South African clawed frog. GBH treatment leads to reduced body length and mobility, smaller eyes, brains and cranial cartilages, and abnormalities in cardiac development. GBH also affects the expression of marker genes in different tissues and developmental stages.
AQUATIC TOXICOLOGY
(2022)
Article
Developmental Biology
Thomas Naert, Ozgun Cicek, Paulina Ogar, Max Burgi, Nikko-Ideen Shaidani, Michael M. Kaminski, Yuxiao Xu, Kelli Grand, Marko Vujanovic, Daniel Prata, Friedhelm Hildebrandt, Thomas Brox, Olaf Ronneberger, Fabian F. Voigt, Fritjof Helmchen, Johannes Loffing, Marko E. Horb, Helen Rankin Willsey, Soeren S. Lienkamp
Summary: This study demonstrates the use of deep learning and light-sheet microscopy to automate segmentation tasks in various imaging modalities, quantitatively assess phenotypes of altered embryonic development, and enhance the sensitivity and throughput of evaluating developmental malformations. The combination of these tools provides a versatile framework for higher-throughput characterization of embryonic model organisms.
Article
Genetics & Heredity
Qiting Yao, Shi Duan, Qing Yang, Xiaoqin Ma, Zhenyu Li, Kongyue Wu, Pei Chang, Mengxi Cao, Xinhua Chen, Zequn Wang, Xueping Zhong, Qingchun Zhou, Haobin Zhao
Summary: This study reveals the critical role of Mep50 in medaka embryonic development. Loss of Mep50 leads to abnormal embryogenesis and affects the maintenance of stem cells and inhibition of apoptosis.
Article
Biochemistry & Molecular Biology
Julia Arand, H. Rosaria Chiang, David Martin, Michael P. Snyder, Julien Sage, Renee A. Reijo Pera, Mark Wossidlo
Summary: Tet enzymes play crucial roles in mouse embryonic development, with Tet2 deficiency showing the most severe phenotype. They function in the consecutive oxidation of 5-methylcytosine to 5-carboxylcytosine, necessary for embryonic genome activation and expression of transposable elements.
Article
Multidisciplinary Sciences
Julia Locke, Agnel Praveen Joseph, Alejandro Pena, Martin M. Moeckel, Thomas U. Mayer, Maya Topf, Carolyn A. Moores
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biology
Martin M. Moeckel, Andreas Heim, Thomas Tischer, Thomas U. Mayer
Article
Biochemistry & Molecular Biology
Andreas Heim, Thomas Tischer, Thomas U. Mayer
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Biochemistry & Molecular Biology
Lars Henschke, Matthias Frese, Susanne Hellmuth, Andreas Marx, Olaf Stemmann, Thomas U. Mayer
ACS CHEMICAL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Suyang Zhang, Thomas Tischer, David Barford
NATURE COMMUNICATIONS
(2019)
Article
Biochemistry & Molecular Biology
P. Ripani, J. Delp, K. Bode, M. E. Delgado, L. Dietrich, V. M. Betzler, N. Yan, G. von Scheven, T. U. Mayer, M. Leist, T. Brunner
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Biochemistry & Molecular Biology
Claudio Alfieri, Thomas Tischer, David Barford
Article
Multidisciplinary Sciences
Mami Oikawa, Angela Simeone, Eva Hormanseder, Marta Teperek, Vincent Gaggioli, Alan O'Doherty, Emma Falk, Matthieu Sporniak, Clive D'Santos, Valar Nila Roamio Franklin, Kamal Kishore, Charles R. Bradshaw, Declan Keane, Thomas Freour, Laurent David, Adrian T. Grzybowski, Alexander J. Ruthenburg, John Gurdon, Jerome Jullien
NATURE COMMUNICATIONS
(2020)
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Cell Biology
Stanislau Yatskevich, Jessie S. Kroonen, Claudio Alfieri, Thomas Tischer, Anna C. Howes, Linda Clijsters, Jing Yang, Ziguo Zhang, Kaige Yan, Alfred C. O. Vertegaal, David Barford
Summary: SUMOylation of the APC/C rearranges the structure of the WHB domain of the cullin subunit, leading to reduced affinity of APC/C-Cdc20 for the mitotic checkpoint complex and allowing for more efficient ubiquitination of substrates in the presence of the MCC, contributing to timely anaphase onset.
Article
Cell Biology
Eva Hoermanseder
Summary: The stability of cell fates during development involves differentiation, cell memory, and the potential for reprogramming. Epigenetic mechanisms play a crucial role in maintaining stable cell-fate memory and resistance to reprogramming, highlighting the importance of understanding these processes in regenerative medicine.
CURRENT OPINION IN GENETICS & DEVELOPMENT
(2021)
Article
Cell Biology
Thomas Tischer, Jing Yang, David Barford
Summary: The control of protein abundance is critical during mitosis, and this study reveals that the APC/C not only regulates mitotic progression, but also plays a role in spindle assembly through its interaction with Cep152.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Stanislau Yatskevich, Kyle W. Muir, Dom Bellini, Ziguo Zhang, Jing Yang, Thomas Tischer, Masa Predin, Tom Dendooven, Stephen H. McLaughlin, David Barford
Summary: This study reveals that CCAN can bind to CENP-A(Nuc) and form a robust structure around the linker DNA, enabling kinetochores to withstand forces exerted by the mitotic spindle.
Article
Cell & Tissue Engineering
Eva Hormanseder, Angela Simeone, George E. Allen, Charles R. Bradshaw, Magdalena Figlmuller, John Gurdon, Jerome Jullien