Article
Microbiology
Bradley Benjamin, Angad Garg, Nikolaus Jork, Henning J. Jessen, Beate Schwer, Stewart Shuman
Summary: We studied the biochemical properties and mutations of the autonomous Asp1 kinase domain in fission yeast. The results showed that under optimal kinase conditions, the reaction of Asp1 kinase with IP6 and ATP resulted in both IP6 phosphorylation to 1-IP7 and hydrolysis of the ATP gamma-phosphate with near-equal partitioning between productive 1-IP7 synthesis and unproductive ATP hydrolysis.
Review
Nutrition & Dietetics
Mariano Bizzarri, Noemi Monti, Aurora Piombarolo, Antonio Angeloni, Roberto Verna
Summary: Myo-inositol is the most abundant natural polyol among the nine structural isomers. It plays a crucial role in biochemical processes governing cellular transitions and insulin transduction. It also affects glucose metabolism and steroid hormone synthesis, and has been successfully used in the management of polycystic ovarian syndrome.
Article
Biochemistry & Molecular Biology
Kevin Ritter, Nikolaus Jork, Anne-Sophie Unmuessig, Maja Koehn, Henning J. J. Jessen
Summary: Inositol phosphates, a family of highly charged messenger molecules, have diverse functions in cellular processes. The phosphorylation patterns result in a vast array of compounds. This study presents a method using NMR measurements for enantiomer assignment, enabling the differentiation of enantiomers. The method was applied to various inositol phosphates and will aid in the assignment of biologically relevant isomers.
Article
Chemistry, Multidisciplinary
Huacan Lin, Luke J. Leman, Ramanarayanan Krishnamurthy
Summary: Researchers have developed a chemical phosphorylation method that allows the synthesis of pyrophosphopeptides in aqueous medium without the need for protecting groups. The method uses diamidophosphate (DAP) for a one-pot sequential phosphorylation-hydrolysis reaction of mono-phosphorylated peptide precursors. The researchers demonstrate the successful synthesis of pyrophosphopeptides in various model peptides and showcase the potential of this method.
Article
Chemistry, Multidisciplinary
Sarah Hostachy, Huanchen Wang, Guangning Zong, Katy Franke, Andrew M. Riley, Peter Schmieder, Barry V. L. Potter, Stephen B. Shears, Dorothea Fiedler
Summary: Inositol pyrophosphates (PP-IPs) are heavily phosphorylated messenger molecules that play important roles in various biological processes. The synthesis of stable analogs and the evaluation of their properties using different methods provide insights into the function and regulation of PP-IPs. This study reports the synthesis of a new reagent and the development of a fluorinated analog of 5PP-IP5. The results demonstrate the significant impact of small structural alterations on the properties of the analogs, which will guide future investigations.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Review
Pharmacology & Pharmacy
Michael L. Jennings
Summary: This review summarizes the role of plasma membrane transporters in regulating intracellular inorganic phosphate (Pi) levels in mammals. The influx of Pi is mediated by SLC34 and SLC20 Na+-Pi cotransporters, while efflux is balanced by XPR1. Two new pathways for mammalian Pi transport regulation have been discovered, involving the recycling of PiT1 and the binding of InsP8 to XPR1. Published data suggests that the regulation of Pi levels varies between different cells and organs, and more research is needed to fully understand how mammalian Pi homeostasis is maintained.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Microbiology
Ana M. Sanchez, Beate Schwer, Nikolaus Jork, Henning J. Jessen, Stewart Shuman
Summary: Inositol pyrophosphate 1,5-IP8 is a signaling molecule that regulates phosphate and polyphosphate homeostasis. Siw14 is identified as a pyrophosphatase involved in inositol pyrophosphate catabolism. Siw14 and Aps1 pyrophosphatases have redundant functions in fission yeast, and high levels of 1,5-IP8 are toxic for the cell.
Article
Multidisciplinary Sciences
Danye Qiu, Verena B. Eisenbeis, Adolfo Saiardi, Henning J. Jessen
Summary: Inositol pyrophosphates are important intracellular signaling molecules, and a detailed protocol for their quantitation in mammalian cells using CE-ESI-MS has been presented. This method allows sensitive profiling of all relevant species and monitoring of their temporal changes under various conditions.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Multidisciplinary Sciences
Beate Schwer, Angad Garg, Agata Jacewicz, Stewart Shuman
Summary: The study reveals a mechanism by which Pol2 termination is enhanced through a gain-of-function mutation G476S in the RNA-binding domain, reducing transcriptional interference to derepress specific genes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell Biology
Sophie Lev, Bethany Bowring, Desmarini Desmarini, Julianne Teresa Djordjevic
Summary: This review provides an overview of the regulation of cellular processes by IPs and PP-IPs in eukaryotes, highlighting their interactions with specific protein targets. Technological advances in genetics have expanded studies to microbial pathogens, showing the significant impact of disrupting PP-IP biosynthesis and PP-IP-protein interactions on pathogenicity. The study also reveals instances of poor conservation in the outcomes of IP-protein interactions despite the presence of conserved IP-binding domains in eukaryotic proteomes.
CELLULAR MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Esther Riemer, Danye Qiu, Debabrata Laha, Robert K. Harmel, Philipp Gaugler, Verena Gaugler, Michael Frei, Mohammad-Reza Hajirezaei, Nargis Parvin Laha, Lukas Krusenbaum, Robin Schneider, Adolfo Saiardi, Dorothea Fiedler, Henning J. Jessen, Gabriel Schaaf, Ricardo F. H. Giehl
Summary: This study provides comprehensive insights into how inositol (pyro)phosphate metabolism is regulated by Pi and dependent on the inositol phosphate kinase ITPK1. The research demonstrates that inositol pyrophosphates are more responsive to Pi than lower inositol phosphates and can be separated in different isomers in plants. Additionally, the study identifies new regulatory inositol pyrophosphates and their synthesis with Pi-dependent changes in nutritional and energetic states.
Article
Microbiology
Bradley Benjamin, Yehuda Goldgur, Nikolaus Jork, Henning J. J. Jessen, Beate Schwer, Stewart Shuman
Summary: The fission yeast Schizosaccharomyces pombe phosphate regulon is sensitive to the level of the inositol pyrophosphate signaling molecule 1,5-IP8. The enzyme Asp1, consisting of a kinase domain and a pyrophosphatase domain, plays a crucial role in determining the dynamics of IP8. Crystal structures of the Asp1 kinase domain reveal its conformational changes upon ligand binding and its substrate specificity. Wild-type Asp1 kinase can utilize N-6-benzyl-ATP as a phosphate donor.
Review
Agronomy
Tao Wu, Chuang Wang, Bei Han, Zhu Liu, Xinyu Yang, Wei Wang, Guangda Ding, John P. Hammond, Philip J. White, Fangsen Xu, Lei Shi
Summary: Phosphorus (P) is essential for plant growth and development, and inositol pyrophosphates (PP-InsPs) serve as important nutrient messengers in signaling P status. This review highlights the biosynthesis of PP-InsPs and their regulation by plant P status, the impact of PP-InsPs on SPX domain-containing proteins in P status signaling, the effects of inositol pyrophosphates on auxin and jasmonate signaling, and the potential crosstalk between P status signaling and phytohormone signaling mediated by inositol pyrophosphates. The interaction between inositol pyrophosphates and their binding proteins is crucial for plant P status and developmental responses to different P supply.
Article
Microbiology
Juan Tian, Mengli Pu, Bin Chen, Guangda Wang, Chunli Li, Xiaxia Zhang, Yanjun Yu, Zhi Wang, Zhaosheng Kong
Summary: It has been discovered that the VdAsp1 gene, encoding an inositol polyphosphate kinase, is essential for the pathogenicity of Verticillium dahliae. Loss of function of VdAsp1 does not affect host invasion but impairs colonization and proliferation in vascular tissues. This study reveals that VdAsp1 regulates the transition from vegetative growth to asexual reproduction by modulating microtubule dynamic organization, which is crucial for V. dahliae to colonize and proliferate in vascular tissues.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Tyler Heitmann, James C. Barrow
Summary: Inositol is a small biological molecule that can undergo phosphorylation and play a role in various cellular processes. IP6K, one of the enzymes involved in inositol phosphate signaling pathway, converts inositol to pyrophosphate and is important in cell signaling, metabolic disease, and central nervous system function.
Article
Biochemistry & Molecular Biology
Cristina Azevedo, Thomas Livermore, Adolfo Saiardi
Article
Biochemistry & Molecular Biology
Debabrata Laha, Philipp Johnen, Cristina Azevedo, Marek Dynowski, Michael Weiss, Samanta Capolicchio, Haibin Mao, Tim Iven, Merel Steenbergen, Marc Freyer, Philipp Gaugler, Marilia K. F. de Campos, Ning Zheng, Ivo Feussner, Henning J. Jessen, Saskia C. M. Van Wees, Adolfo Saiardi, Gabriel Schaaf
Correction
Biochemistry & Molecular Biology
Cristina Azevedo, Adolfo Saiardi
BIOCHEMICAL SOCIETY TRANSACTIONS
(2016)
Review
Biochemistry & Molecular Biology
Thomas M. Livermore, Cristina Azevedo, Bernadett Kolozsvari, Miranda S. C. Wilson, Adolfo Saiardi
BIOCHEMICAL SOCIETY TRANSACTIONS
(2016)
Review
Biochemistry & Molecular Biology
Cristina Azevedo, Adolfo Saiardi
BIOCHEMICAL SOCIETY TRANSACTIONS
(2016)
Review
Biochemistry & Molecular Biology
Cristina Azevedo, Adolfo Saiardi
TRENDS IN BIOCHEMICAL SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Cristina Azevedo, Jyoti Singh, Nicole Steck, Alexandre Hofer, Felix A. Ruiz, Tanya Singh, Henning J. Jessen, Adolfo Saiardi
ACS CHEMICAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Cristina Azevedo, Adolfo Saiardi
BIOCHEMICAL SOCIETY TRANSACTIONS
(2014)
Article
Biochemistry & Molecular Biology
Annalisa Lonetti, Zsolt Szijgyarto, Daniel Bosch, Omar Loss, Cristina Azevedo, Adolfo Saiardi
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Cell Biology
Sudeshna Nag, Shikha Rani, Sarmistha Mahanty, Christin Bissig, Pooja Arora, Cristina Azevedo, Adolfo Saiardi, Peter van der Sluijs, Cedric Delevoye, Guillaume van Niel, Graca Raposo, Subba Rao Gangi Setty
JOURNAL OF CELL SCIENCE
(2018)
Article
Multidisciplinary Sciences
Adam Burton, Cristina Azevedo, Catia Andreassi, Antonella Riccio, Adolfo Saiardi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2013)
Review
Biochemistry & Molecular Biology
Yann Desfougeres, Adolfo Saiardi, Cristina Azevedo
BIOCHEMICAL SOCIETY TRANSACTIONS
(2020)
Article
Biochemistry & Molecular Biology
Cristina Azevedo, Yann Desfougeres, Yannasittha Jiramongkol, Hamish Partington, Sasanan Trakansuebkul, Jyoti Singh, Nicole Steck, Henning J. Jessen, Adolfo Saiardi
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Claudia Rato, Miguel F. Carvalho, Cristina Azevedo, Paula Rodrigues Oblessuc
Summary: Conventional crop breeding struggles to meet the increasing food demands and evolving pests and pathogens, compounded by global climate changes. Genome editing technologies, such as CRISPR/Cas, offer targeted breeding solutions to enhance plant resistance to pests and pathogens, accelerating the breeding process.
TRANSGENIC RESEARCH
(2021)
Article
Microbiology
Brian S. Mantilla, Cristina Azevedo, Paul W. Denny, Adolfo Saiardi, Roberto Docampo
Summary: This study reveals the crucial role of histidine ammonia lyase (HAL) in the alkalinization of acidocalcisomes by binding to polyphosphate and regulating its function through lysine residues in the C-terminal region. HAL localization and inhibition by polyP demonstrate a mechanism for acidocalcisome alkalinization linked to amino acid metabolism.