Article
Food Science & Technology
Congcong Li, Zengfeng Zhang, Xuebin Xu, Shoukui He, Xiaodong Zhao, Yan Cui, Xiujuan Zhou, Chunlei Shi, Yanhong Liu, Min Zhou, Xianming Shi
Summary: Studies on 162 Salmonella Enteritidis isolates collected from child patients in China showed that some isolates carried cephalosporin resistance genes and had the possibility of clonal spread. Conjugation experiments confirmed the presence of bla(CTX-M-55) on conjugative plasmids, which might facilitate the spread of cephalosporin resistance in Salmonella Enteritidis.
FOODBORNE PATHOGENS AND DISEASE
(2021)
Article
Microbiology
Eva A. Edward, Nelly M. Mohamed, Azza S. Zakaria
Summary: Egypt has recently experienced the emergence of multidrug-resistant Klebsiella pneumoniae, presenting a significant challenge to healthcare. The rapid dissemination of bla(CTX-M) genes, particularly bla(CTX-M-14) and bla(CTX-M-15), among these multidrug-resistant K. pneumoniae has been observed. This study investigates the occurrence of bla(CTX-M-IV) among K. pneumoniae isolates from a major hospital in Alexandria, Egypt. Approximately 22% of the tested isolates were found to carry the bla(CTX-M-IV) gene. Whole genome sequencing revealed the presence of high-risk clone ST383 in one of the isolates, which carried bla(CTX-M-14) on an IncL/M plasmid and bla(CTX-M-15) on a hybrid IncHI1B/IncFIB plasmid. These plasmids showed similarities to those found in other countries such as the UK, Germany, and China. The presence of plasmids with enhanced genetic plasticity poses a significant threat as it allows the evolution of K. pneumoniae isolates causing untreatable infections. Nationwide surveillance is urgently needed to monitor the prevalence of these superbugs with limited treatment options.
Article
Medicine, Research & Experimental
Christopher O. Fakorede, Kehinde O. Amisu, Morteza Saki, Kabiru O. Akinyemi
Summary: This study investigated antibiotic resistance and the carriage of bla(CTX-M-9) and bla(CTX-M-15) among Salmonella species from hospitalised patients in Lagos, Nigeria. It found a high level of resistance to cephalosporins, penicillin, and some carbapenems, indicating the need for prudent use of cephalosporins.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Article
Infectious Diseases
Matheus Silva Sanches, Luana Carvalho Silva, Caroline Rodrigues da Silva, Victor Hugo Montini, Bruno Henrique Dias de Oliva, Gustavo Henrique Migliorini Guidone, Mara Correa Lelles Nogueira, Maisa Fabiana Menck-Costa, Renata Katsuko Takayama Kobayashi, Eliana Carolina Vespero, Sergio Paulo Dejato Rocha
Summary: The study aimed to evaluate the prevalence of antimicrobial resistance and clonal relationships in Proteus mirabilis isolated from different sources. Results showed that chicken meat had the highest multidrug resistance, followed by pork and community-acquired urinary tract infections (UTI-CA), while beef had relatively few multidrug-resistant strains. Various antimicrobial resistance genes were detected, especially in chicken meat and UTI-CA isolates. Molecular typing revealed genetic similarity between chicken meat and UTI-CA isolates, indicating that chicken could be a source of P. mirabilis causing UTI-CA. It was concluded that meat, especially chicken, can be an important source of multidrug-resistant P. mirabilis dissemination in the community.
Article
Agriculture, Dairy & Animal Science
Rajib Deb, Parul Chaudhary, Sachinandan De
Summary: CTX-M beta-lactamases are important resistance enzymes in E. coli, and a high prevalence of ESBL-positive E. coli isolates was found in mastitis cow milk. The resistance rates to various beta-lactam antibiotics were also high, and targeting the (CTX)-C-bla-M gene using CRISPR/Cas9 technology revealed changes in antibiotic susceptibility.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Infectious Diseases
Yinping Wang, Jintao He, Long Sun, Yan Jiang, Lihua Hu, Sebastian Leptihn, Pengfei Zhu, Xiaoting Fu, Yunsong Yu, Xiaoting Hua
Summary: This study characterized two Escherichia coli strains isolated from a patient before and after treatment, showing increased resistance and gene copy number variation in the post-treatment strain. The increased gene copy number did not impose fitness costs on the strain.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Microbiology
Shirong Li, Siquan Shen, Li Ding, Renru Han, Yan Guo, Dandan Yin, Ming Guan, Fupin Hu
Summary: The prevalence of carbapenem-resistant Klebsiella pneumoniae is increasing, with reports of non-carbapenemase-producing K. pneumoniae showing high-level resistance to carbapenems. In this study, a carbapenemase-negative carbapenem-resistant K. pneumoniae was isolated from a patient, and resistance genes and plasmid transfer were detected. This study reveals the resistance mechanism of this strain.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Kwang Won Seo, Young Ju Lee
Summary: This study investigated the prevalence and characteristics of third-generation cephalosporin-resistant and CTX-M-producing E. coli in broiler parent stock in Korea, highlighting the importance of comprehensive surveillance and monitoring systems to prevent the dissemination of resistant isolates.
Article
Agriculture, Dairy & Animal Science
Wenya Li, Yinshu Li, Yating Jia, Huarun Sun, Chunhui Zhang, Gongzheng Hu, Li Yuan
Summary: The transmission characteristics of mcr-1 and bla(CTX-M-type) in the LWY24 isolate are influenced by clonal spread of ST93, selective pressure of cefotaxime, IS26-mediated homologous recombination and transposition, as well as the specific shufflon region. The study also showed that the mcr-1 and bla(CTX-M-55) carrying plasmids could be co-transferred at a similar frequency under selection pressure, indicating potential horizontal transfer mechanisms.
Article
Agriculture, Multidisciplinary
Wang Wen-jing, Wang Yi-fu, Jin Ya-jie, Song Wu-qiang, Lin Jia-meng, Zhang Yan, Tong Xin-ru, Tu Jian, Li Rui-chao, Li Tao
Summary: An extensively drug-resistant (XDR) Escherichia coli strain 258E was identified in an anal swab sample from a chicken farm in Anhui Province, China. The strain 258E carries an IncN plasmid pEC258-3, which contains multiple resistance genes and is highly similar to a plasmid found in Klebsiella pneumoniae. Conjugation experiments confirm the horizontal transferability of plasmid pEC258-3, posing a significant potential threat in animals, community, and hospital settings.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2023)
Article
Microbiology
Kun He, Wenya Li, Bing Zhao, Hui Xu, Yushan Pan, Dandan He, Gongzheng Hu, Hua Wu, Li Yuan
Summary: This study investigated the interaction between bla(CTX-M)-bearing IncFII and mcr-1-carrying IncI2 plasmids in bacterial hosts, revealing that coinhabitant plasmids are more stable, provide more fitness advantages, and are easier to transfer and cotransfer than single plasmids. Coinhabitant FII and I2 plasmids play an important role in driving the persistence and spread of plasmid-carried bla(CTX-M) and mcr-1 genes in clinical E. coli, posing a serious threat to clinical therapy and public health.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
L. Saiz-Escobedo, I. Cadenas-Jimenez, R. Olmos, A. Carrera-Salinas, D. Berbel, J. Camara, F. Tubau, M. A. Dominguez, C. Ardanuy, A. Gonzalez-Diaz, S. Marti
Summary: Four cephalosporin-resistant H. parainfluenzae strains from patients with urethritis were found to be multidrug-resistant, and a novel ESBL resistance gene was identified. The transmission potential of these resistance genes to other pathogens is a cause for concern.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Immunology
Zhen-Yu Wang, Yue Jiang, Yi-Qiao Shao, Heng-Fan Lu, Meng-Jun Lu, Xinan Jiao, Jing Wang, Qiu-Chun Li
Summary: This study investigates the prevalence and dissemination mechanism of ESBL-producing E. coli from healthy individuals in a community in Yangzhou, China. The study found nasal colonization of CTX-M-55-producing E. coli strains among healthy individuals in the community, indicating a potential risk of antimicrobial resistance dissemination through close contact or environmental transmission.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Wei Zheng, Meina Yue, Jun Zhang, Zhi Ruan
Summary: This study reports the first complete genome sequence of a multidrug-resistant Escherichia coli carrying bla (NDM-5) and two copies of bla (CTX-M-14) in China. The strain belongs to ST354 and contains six plasmids, including a 46161-bp IncX3 plasmid and a 96499-bp IncB plasmid. The closest relative of this E. coli isolate was found in Lebanon, differing by 162 cgMLST loci.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Microbiology
Lili Li, Rikke Heidemann Olsen, Jian Xiao, Hecheng Meng, Shifu Peng, Lei Shi
Summary: This study is the first to report a multidrug-resistant Salmonella enterica serovar Saintpaul isolate recovered from fish in China that harbors resistance genes to fluoroquinolones and extended-spectrum cephalosporins. The study identified the presence of acquired antibiotic resistance genes and mobile genetic elements on a plasmid, as well as the transferability of these resistance genes to Escherichia coli. Genome sequence comparisons linked the isolate with strains from the United Kingdom.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Lucy A. Brunton, Neil Alexander, William Wint, Adam Ashton, Jennifer M. Broughan
STOCHASTIC ENVIRONMENTAL RESEARCH AND RISK ASSESSMENT
(2017)
Editorial Material
Veterinary Sciences
K. A. Harris, L. Brunton, A. Brouwer, M. P. Romero Garcia, J. C. Gibbens, N. H. Smith, P. A. Upton
Article
Environmental Sciences
Lucy A. Brunton, Andrew P. Desbois, Maria Garza, Barbara Wieland, Chadag Vishnumurthy Mohan, Barbara Hasler, Clarence C. Tam, Phuc Nguyen Thien Le, Nguyen Thanh Phuong, Phan Thi Van, Hung Nguyen-Viet, Mahmoud M. Eltholth, Dang Kim Pham, Phuc Pham Duc, Nguyen Tuong Linh, Karl M. Rich, Ana L. P. Mateus, Md Ahasanul Hoque, Abdul Ahad, Mohammed Nurul Absar Khan, Alexandra Adams, Javier Guitian
SCIENCE OF THE TOTAL ENVIRONMENT
(2019)
Article
Multidisciplinary Sciences
Sara H. Downs, Alison Prosser, Adam Ashton, Stuart Ashfield, Lucy A. Brunton, Adam Brouwer, Paul Upton, Andrew Robertson, Christl A. Donnelly, Jessica E. Parry
SCIENTIFIC REPORTS
(2019)
Article
Veterinary Sciences
M. Pilar Romero, Yu-Mei Chang, Lucy A. Brunton, Jessica Parry, Alison Prosser, Paul Upton, Eleanor Rees, Oliver Tearne, Mark Arnold, Kim Stevens, Julian A. Drewe
PREVENTIVE VETERINARY MEDICINE
(2020)
Article
Immunology
Gareth Enticott, Damian Maye, Rhiannon Naylor, Lucy Brunton, Sara H. Downs, Christl A. Donnelly
Article
Veterinary Sciences
M. Pilar Romero, Yu-Mei Chang, Lucy A. Brunton, Alison Prosser, Paul Upton, Eleanor Rees, Oliver Tearne, Mark Arnold, Kim Stevens, Julian A. Drewe
Summary: Machine learning approaches applied to big datasets offer a potential way to improve our understanding of bTB risk and enhance the current disease control policy.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Infectious Diseases
Richard Kock, Anita L. Michel, Dorothy Yeboah-Manu, Esam I. Azhar, Jordi B. Torrelles, Simeon I. Cadmus, Lucy Brunton, Jeremiah M. Chakaya, Ben Marais, Leonard Mboera, Zeaur Rahim, Najmul Haider, Alimuddin Zumla
Summary: The article highlights the ongoing issue of zoonotic TB in some regions and countries despite slow reductions in active human tuberculosis cases, emphasizing the need for more attention. Control methods include detecting and culling M. bovis infection in cattle.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Fisheries
Andrew P. Desbois, Maria Garza, Mahmoud Eltholth, Yamen M. Hegazy, Ana Mateus, Alexandra Adams, David C. Little, Erling Hog, Chadag Vishnumurthy Mohan, Shimaa E. Ali, Lucy A. Brunton
Summary: This study applies participatory systems-thinking approach to Nile tilapia production in the Nile Delta of Egypt, identifying potential short-term interventions to effectively reduce antibiotic use, mainly focusing on improving culture water quality parameters, feed storage conditions, and training programs for stakeholders.
Article
Infectious Diseases
Maria Garza, Chadag Mohan, Lucy Brunton, Barbara Wieland, Barbara Hasler
Summary: This study aims to develop a typology to classify and characterize interventions to reduce antimicrobial use in aquaculture and identify points of action. The most common interventions to address antimicrobial use and resistance were legislative and regulatory frameworks and voluntary instruments. Specific interventions targeting antimicrobial use and resistance were scarce. The presented typology can guide evaluation of interventions that promote responsible antimicrobial use and inform the design of future interventions.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Veterinary Sciences
M. Pilar Romero, Yu-Mei Chang, Lucy A. Brunton, Jessica Parry, Alison Prosser, Paul Upton, Julian A. Drewe
Summary: This study highlights the inadequate sensitivity of diagnostic testing as a major obstacle in the eradication of bovine tuberculosis in England. By using machine learning algorithms, at-risk tests can be identified to strengthen monitoring measures and mitigate the risk of disease spread.
PREVENTIVE VETERINARY MEDICINE
(2022)
Article
Microbiology
Solomon O. Olorunleke, Miranda Kirchner, Nicholas Duggett, Manal AbuOun, Onyinye J. Okorie-Kanu, Kim Stevens, Roderick M. Card, Kennedy Foinkfu Chah, John A. Nwanta, Lucy A. Brunton, Muna F. Anjum
Summary: The study found a widespread presence of antimicrobial-resistant Escherichia coli in livestock and in-contact humans in Southeast Nigeria, highlighting the potential threat to livestock industry and human health. Whole-genome sequencing revealed high genetic diversity among the isolates, with some carrying multiple antibiotic resistance genes. Surprisingly, human isolates had the highest number of resistance genes, while pigs had the least.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
D. Elkholly, A. Fraser, R. Booth, D. O'Neill, A. Mateus, L. Brunton, D. Brodbelt
Summary: Antimicrobial resistance (AMR) is a growing health problem that requires monitoring of antimicrobial usage in farm animals. A study in the UK estimated the usage of antimicrobials and critically important antimicrobials (HPCIAs) using electronic clinical records. Veterinarians were interviewed to explore barriers and facilitators in relation to antimicrobial use.
PREVENTIVE VETERINARY MEDICINE
(2023)
Article
Geography
G. Enticott, K. Ward, A. Ashton, L. Brunton, J. Broughan
Article
Veterinary Sciences
Elizabeth May, Alison Prosser, Sara H. Downs, Lucy A. Brunton
VETERINARY SCIENCES
(2019)
Article
Veterinary Sciences
R. Mischke, K. Rumstedt, S. O. Hungerbuehler, K. Rohn, M. Schmicke
Summary: Contrast-enhanced ultrasound (CEUS) can provide an additional tool for the diagnosis of hypothyroidism in dogs and support its differentiation from non-thyroidal illness. Sedation has limited impact on CEUS results.
RESEARCH IN VETERINARY SCIENCE
(2024)
Review
Veterinary Sciences
Lucas Petitemberte de Souza, William Borges Domingues, Eduardo Bierhals Blodorn, Leandro da Silva Nunes, Hadassa Gabriela Ortiz, Eliza Rossi Komninou, Vinicius Farias Campos
Summary: This study performed a systematic review of the differential expression of sperm microRNAs in bulls related to fertility. A total of 182 unique differentially expressed miRNAs were identified, among which 49 miRNAs were found in common between at least two studies. The results suggest that miRNAs such as miR-10a, -10b, -103, -15b, -122, -125b, -126-5p, -151-5p, -193a-5p, -196a, -27a-5p, and -99b may serve as potential universal biomarkers for assessing the reproductive potential of males.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Wonhee Cha, Katinca Fungbrant, Giulio Grandi, Ylva Persson
Summary: This study investigated the prevalence and development of hair loss in outdoor cattle in Sweden. It found a relatively high hair loss rate, with the occurrence of hair loss increasing over time during winters, suggesting a contagious etiology. The study also identified preventative delousing before November, keeping animals clean, and maintaining small group sizes as effective measures to reduce the risk of hair loss, while older age and access to bedding materials increased the risk.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Sheeba Thomas, David Francis Lappin, David Bennett, Christopher Nile, Marcello Pasquale Riggio
Summary: This study found that Feline odontoclastic resorptive lesion (FORL) can be divided into two subgroups, with FORL-1 subgroup having a less diverse oral microbiome and higher levels of proinflammatory cytokines and chemokines, which may be associated with the severity and activity of the disease.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
S. L. Gayathri, M. Bhakat, T. K. Mohanty
Summary: This study used infrared thermography (IRT) to identify mastitis in Sahiwal cows. The results showed significant differences in the mean values of udder and teat surface temperature between healthy, subclinical mastitis (SCM), and clinical mastitis (CM) cases during different seasons. IRT is an efficient tool for the early identification of subclinical mastitis.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Zeynep Ozdemir Kutahya, Busra Aslan Akyol, Kubra Deliklitas, Cengiz Gokbulut
Summary: In this study, the pharmacokinetic behavior of Eprinomectin was studied in two different goat breeds. The results showed no significant differences in plasma concentration between the breeds, but the clearance from the body may vary. High concentration of Eprinomectin in feces is beneficial against gastrointestinal parasites but poses an ecotoxicological risk to the environment.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Mohammad Ashrafzadeh-Shiraz, Mousa Tavassoli, Bahram Dalir-Naghadeh, Alireza Sazmand
Summary: This study evaluated the efficacy of fenbendazole and ivermectin on strongyles and Parascaris sp. infecting adult riding horses in different regions of Iran. The results demonstrated varying degrees of resistance of these parasites to both anthelminthics. It emphasizes the urgency of educating horse owners about the importance of a test-and-treatment strategy and suggests evaluating the efficacy of different anthelminthics to choose the most effective treatment in each region.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Moises Gonzalvez, Clara Munoz-Hernandez
Summary: The study evaluated the use of R software in research articles addressing the study of wildlife, showing an increasing trend in the number of publications over time and a wide geographical distribution globally.
RESEARCH IN VETERINARY SCIENCE
(2024)