Article
Biochemistry & Molecular Biology
Alina A. Sofronova, Denis V. Pozdyshev, Kseniya V. Barinova, Vladimir I. Muronetz, Pavel I. Semenyuk
Summary: The study found that glycation of GAPDH inhibits its interaction with alpha-synuclein and RNA, potentially influencing the interplay between diabetes and neurodegenerative diseases.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Chemistry, Multidisciplinary
Astrid M. Perez, Jacob A. Wolfe, Janse T. Schermerhorn, Yiwen Qian, Bekim A. Cela, Cody R. Kalinowski, Garrett E. Largoza, Peter A. Fields, Gabriel S. Brandt
Summary: This study found that the thermal stability of a critical metabolic enzyme in the stony coral Acropora millepora significantly increases in the presence of its cofactor NAD(+). The determination of the complex structure revealed variable NAD(+) occupancy across the tetrameric enzyme, with regions of difference identified that may account for the increased thermal stability.
Article
Microbiology
Shotaro Isozaki, Hiroaki Konishi, Hiroki Tanaka, Chikage Yamamura, Kentaro Moriichi, Naoki Ogawa, Mikihiro Fujiya
Summary: This study demonstrated that heptelidic acid (HA) derived from the probiotic Aspergillus oryzae exerts antitumor effects against extraintestinal melanoma cells both in vitro and in vivo. Oral administration of HA significantly suppressed the growth of transplanted melanoma tumors, indicating a potential novel system for regulating extraintestinal tumors through gut bacteria-derived bioactive mediators.
Article
Plant Sciences
Cong Liu, Yanfeng Wang, Yuanyuan Du, Zhensheng Kang, Jia Guo, Jun Guo
Summary: In this study, a Pst effector protein was found to manipulate plant defense by interacting with key plant proteins. This effector protein regulated two different ROS signaling pathways by interacting with two wheat proteins, affecting the plant's resistance to Pst. Additionally, the effector protein stabilized a protein that suppresses ROS accumulation by inhibiting protein degradation. These findings revealed the mechanism behind how Pst promotes its infection by manipulating plant proteins.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Veterinary Sciences
Xiaomeng Chen, Yanan Zhao, Lingling Su, Li Wang, Xiaohui Ma, Baojiang Zhang, Yan Su
Summary: The research selected three S. equi antigens for immunization and challenge experiments, and found that this multi-antigen treatment can effectively enhance immunity and improve protection efficacy, demonstrating its potential as a promising vaccine candidate.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Immunology
Matthew J. Sullivan, Kelvin G. K. Goh, Ruby Thapa, Debasish Chattopadhyay, Deepak S. Ipe, Benjamin L. Duell, Lahiru Katupitiya, Dean Gosling, Dhruba Acharya, Glen C. Ulett
Summary: This study suggests that Streptococcus agalactiae GAPDH affects phagocytosis outcomes and cytokine production, and influences bacterial survival in the female reproductive tract.
Article
Immunology
David Salcines-Cuevas, Hector Teran-Navarro, Ricardo Calderon-Gonzalez, Paula Torres-Rodriguez, Raquel Tobes, Manuel Fresno, Jorge Calvo-Montes, I. Concepcion Perez Del Molino-Bernal, Sonsoles Yanez-Diaz, Carmen Alvarez-Dominguez
Summary: The N-terminal 22 amino acid peptides of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are highly homologous among pathogenic bacteria like Listeria, Mycobacterium, and Streptococcus, making them ideal candidates for universal vaccine designs. Vaccines containing these peptides demonstrated high immunogenicity, lack of toxicity, and cross-protection immunity in mouse models infected with different pathogens.
Review
Polymer Science
Agnieszka Folentarska, Jakub Lagiewka, Magdalena Krystyjan, Wojciech Ciesielski
Summary: This paper investigates the interactions between polysaccharides with different electrical charges (anionic and neutral starches) and proteins and fats in food ingredients, aiming to understand the mechanisms and interdependence between their properties and intermolecular interactions. It fills the gap in research on ternary blends of natural food polymers and presents the application of natural biopolymers in the food and non-food industries.
Article
Biochemistry & Molecular Biology
Marina A. Mikeladze, Elizaveta A. Dutysheva, Victor G. Kartsev, Boris A. Margulis, Irina Guzhova, Vladimir F. Lazarev
Summary: Hypoxia can affect the tumorigenicity and survival of cancer cells by targeting proteins like GAPDH and Hsp70. Manipulating the expression or interaction of these proteins may provide a promising strategy to enhance the sensitivity of tumor cells to hypoxic stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Alexandre Gouzy, Claire Healy, Katherine A. Black, Kyu Y. Rhee, Sabine Ehrt
Summary: In acidic pH conditions, Mycobacterium tuberculosis adapts its metabolism to preferentially assimilate lipids, like oleic acid, over carbohydrates. Lack of certain enzymes necessary for lipid assimilation can be lethal to M. tuberculosis under acidic conditions, highlighting the pathogen's ability to alter its carbon diet in response to pH stress.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Ines Baptista, Effrosyni Karakitsou, Jean-Baptiste Cazier, Ulrich L. Guenther, Silvia Marin, Marta Cascante
Summary: Through TKTL1 knockdown experiments, it was found that TKTL1 plays a key role in the metabolic adaptation to hypoxia in THP-1 AML cells by modulating the activities of G6PD and GAPDH, which involve changes in redox capacity and transcriptional regulation of key enzymes and transporters related to glucose, PPP, and amino acid metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agronomy
Karen K. Fugate, Larry G. Campbell, Abbas M. Lafta, John D. Eide, Mohamed F. R. Khan, Chenggen Chu, Fernando L. Finger
Summary: This study identified genes and gene products that affect storage respiration rate in sugarbeet roots by creating sugarbeet lines with different respiration rates and characterizing gene expression differences between these lines. The differentially expressed genes (DEGs) between the lines were found to encode transcription factors, enzymes involved in the respiratory pathway, and other genes with various biological and molecular functions. The high upregulation of genes for glyceraldehyde-3-phosphate dehydrogenase in the high respiring line suggests a potential bottleneck in respiratory substrate production.
Article
Biotechnology & Applied Microbiology
Sang Hyun Kim, Jeong Hyeon Hwang, Hyun Joong Kim, Suk Jin Oh, Hyun Jin Kim, Nara Shin, Sang-Hyoun Kim, Jeong-Hoon Park, Shashi Kant Bhatia, Yung-Hun Yang
Summary: The study developed Clostridium acetobutylicum as a superior biohydrogen producer by introducing glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to enhance hydrogen production. The strain CAC824-G containing gapC showed a 66.3% higher hydrogen production than the wild-type strain, with increased NADH and NADPH pools. Overexpression of gapC resulted in increased hydrogen production even in the presence of inhibitors.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Dirk Roosterman, Graeme Stuart Cottrell
Summary: Schizophrenia is a chronic and severe mental disorder affecting over 20 million people worldwide. Research suggests disturbances in glucose metabolism may be a potential mechanism for the disease, and a 'two-cell' model of glucose metabolism has been developed to explain treatment effects. The model challenges established concepts and suggests schizophrenia can be formulated in two steps involving dysregulation of glucose signaling pathways.
MOLECULAR PSYCHIATRY
(2021)
Article
Environmental Sciences
Mojtaba Ranjbar, Fatemeh Khakdan, Abazar Ghorbani, Meisam Zargar, Moxian Chen
Summary: This study investigated the function of GAPDH in plants under drought-induced stress, focusing on the basil plant. The complete cDNA sequence of basil GAPDH was cloned and its expression patterns were analyzed. The study revealed the evolutionary connections of ObGAPDH and its potential oxidative role in response to stress signals.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ambika M. Murthy, Matthew J. Sullivan, Nguyen Thi Khanh Nhu, Alvin W. Lo, Minh-Duy Phan, Kate M. Peters, Dave Boucher, Kate Schroder, Scott A. Beatson, Glen C. Ulett, Mark A. Schembri, Matthew J. Sweet
Article
Anesthesiology
Stuart M. Brierley, Kelvin G. K. Goh, Matthew J. Sullivan, Kate H. Moore, Glen C. Ulett, Luke Grundy
Article
Immunology
Matthew J. Sullivan, Glen C. Ulett
MICROBIAL PATHOGENESIS
(2020)
Article
Ecology
Deepak S. Ipe, Matthew J. Sullivan, Kelvin G. K. Goh, Saeed M. Hashimi, Alan L. Munn, Glen C. Ulett
Summary: The guaA gene in bacteria encodes an enzyme critical for synthesizing guanine monophosphate, essential for biosynthesis. Lack of guaA affects bacterial colonization in urine. The diversity and organizational structures of guaA in commensal bacteria of the human urogenital tract show cross-phyla characteristics.
Article
Microbiology
Matthew J. Sullivan, Kelvin G. K. Goh, Glen C. Ulett
Summary: The study reveals that Streptococcus agalactiae responds to zinc stress by regulating czcD through sczA, as well as utilizing arginine deamination mechanism mediated by arcA to survive metal intoxication. These systems not only help the bacteria survive zinc stress in vitro, but also enhance virulence during systemic infection in mice, suggesting potential applications in other bacteria facing metal stress in host environments.
Article
Microbiology
Devika Desai, Kelvin G. K. Goh, Matthew J. Sullivan, Debasish Chattopadhyay, Glen C. Ulett
Summary: This study found a higher propensity of hemolytic activity in Group B Streptococcus causing asymptomatic bacteriuria, but no significant differences in biofilm formation abilities among strains with different clinical histories.
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Microbiology
Matthew J. Sullivan, Kelvin G. K. Goh, Dean Gosling, Lahiru Katupitiya, Glen C. Ulett
Summary: The study demonstrates that GBS copA gene mediates resistance to copper intoxication through regulation by the copper-sensing transcriptional repressor copY, and the transcriptional signature in response to copper stress in GBS enhances virulence and contributes to its survival in different environments. Understanding microbial management of metal ions like copper is crucial for explaining bacterial survival mechanisms in stressful conditions.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Microbiology
Thierry Franza, Annika Rogstam, Saravanamuthu Thiyagarajan, Matthew J. Sullivan, Aurelie Derre-Bobillot, Mikael C. Bauer, Kelvin G. K. Goh, Violette Da Cunha, Philippe Glaser, Derek T. Logan, Glen C. Ulett, Claes von Wachenfeldt, Philippe Gaudu
Summary: In the facultative aerobic Gram-positive coccus Streptococcus agalactiae, the transcription repressor Rex interacts with both oxidized and reduced forms of NAD, with NAD(+) concentration being the main effector of its DNA binding. Rex plays a key role in modulating virulence gene expression and carbon metabolism to harvest nutrients from the host, thus contributing to GBS pathogenicity.
Article
Microbiology
Laura Alvarez-Fraga, Minh-Duy Phan, Kelvin G. K. Goh, Nguyen Thi Khanh Nhu, Steven J. Hancock, Luke P. Allsopp, Kate M. Peters, Brian M. Forde, Leah W. Roberts, Matthew J. Sullivan, Makrina Totsika, Scott A. Beatson, Glen C. Ulett, Mark A. Schembri
Summary: Many antibiotic resistant uropathogenic Escherichia coli (UPEC) strains belong to clones defined by their multilocus sequence type (ST), with ST131 being the most dominant. Although we have a good understanding of resistance development to fluoroquinolones and third-generation cephalosporins by ST131, our understanding of the virulence repertoire that has contributed to its global dissemination is limited. Afa/Dr fimbriae genes are found frequently in ST131 strains, and the integration of insertion sequence (IS) elements in the afa regulatory region leads to altered virulence capacity.
Article
Immunology
Matthew J. Sullivan, Kelvin G. K. Goh, Ruby Thapa, Debasish Chattopadhyay, Deepak S. Ipe, Benjamin L. Duell, Lahiru Katupitiya, Dean Gosling, Dhruba Acharya, Glen C. Ulett
Summary: This study suggests that Streptococcus agalactiae GAPDH affects phagocytosis outcomes and cytokine production, and influences bacterial survival in the female reproductive tract.
Article
Microbiology
Kelvin G. K. Goh, Matthew J. Sullivan, Glen C. Ulett
Summary: This study discovered that, in addition to the classical cop operon, GBS utilizes other genes to resist copper stress and maintain copper homeostasis within the cells. These genes include enzymes, cell wall synthesis factors, transporters, and cell signaling factors among others.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Immunology
Anu Chacko, Ali Delbaz, Indra N. Choudhury, Tanja Eindorf, Megha Shah, Christopher Godfrey, Mathew J. Sullivan, James A. St John, Glen C. Ulett, Jenny A. K. Ekberg
Summary: Streptococcus agalactiae can invade the central nervous system via the nose-to-brain path, with increased load after epithelial injury. The bacteria can survive in glia and induce cytokine production, with the capsule affecting cellular responses and survival.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Matthew J. Sullivan, Kelvin G. K. Goh, Glen C. Ulett
Summary: This study reveals a new and important mechanism of cross-system complexity driven by CopY in GBS to regulate cellular management of metal stress and survival.
Article
Multidisciplinary Sciences
Matthew J. Sullivan, Darren Prince, Kelvin G. K. Goh, Lahiru Katupitiya, Dean Gosling, Michael R. Crowley, David K. Crossman, Glen C. Ulett
Summary: A novel system was developed to study the co-transcriptome of intracellular Group B Streptococcus (GBS) and host cells. Analysis of the co-transcriptome revealed dysregulated genes in intracellular GBS and significant remodeling of the monocyte transcriptome. The study identified the Streptococcal Histidine Triad Protein (sht) as a key gene in GBS virulence.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Laura Alvarez-Fraga, Minh-Duy Phan, Kelvin G. K. Goh, Nguyen Thi Khanh Nhu, Steven J. Hancock, Luke P. Allsopp, Kate M. Peters, Brian M. Forde, Leah W. Roberts, Matthew J. Sullivan, Makrina Totsika, Scott A. Beatson, Glen C. Ulett, Mark A. Schembri
Summary: This study identified the widespread presence of genes encoding Afa/Dr fimbriae in UPEC ST131 strains, and demonstrated how the integration of IS elements in the afa regulatory region affects Afa expression, altering its virulence. Evidence suggests that IS1 elements have a more widespread impact on ST131 genome evolution.