Article
Microbiology
Kasey Schalich, Seesandra Rajagopala, Suman Das, Ryan O'Connell, Fang Yan
Summary: This study reveals the regulatory effects of intestinal epithelial cell-derived components on the expression and metabolism of Lactobacillus rhamnosus GG at the molecular level, providing mechanistic insights into the interactions between the gut microbiota and the host.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Yue Shi, Jiao Lin, Xuelian Tao, Jing Qu, Shumin Liao, Mengyao Li, Ke Deng, Ping Du, Kun Liu, Helmut Thissen, Liang Li, Peter Kingshott, Peng-Yuan Wang
Summary: Colloidal self-assembled patterns (cSAPs) are versatile building blocks for generating complex surface patterns with precise control. Functionalized cSAPs have been shown to effectively control cellular responses at biointerfaces, providing instructive insights for biomaterials or biodevices design.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Immunology
Ye Seul Son, Naeun Son, Won Dong Yu, Aruem Baek, Young-Jun Park, Moo-Seung Lee, Seon-Jin Lee, Dae-Soo Kim, Mi-Young Son
Summary: This study investigated the effects of PM10 on inflamed intestines using two-dimensional and three-dimensional human intestinal models. The results showed that PM10 exposure led to pathological changes in the intestinal epithelial cells, exacerbating inflammatory disorders.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Jong Hoon Won, Jacob S. Choi, Joon-Il Jun
Summary: This study reveals that the matricellular protein CCN1 is a niche factor for intestinal stem cells, regulating their proliferation and differentiation through integrin signaling. CCN1 interacts with integrins alpha(v)beta(3)/alpha(v)beta(5) to activate distinct downstream pathways, leading to Notch activation for differentiation via NF-kappaB and Wnt signaling control for proliferation via Src-mediated YAP activation and Dkk1 expression. Moreover, CCN1 and YAP amplify each other's activities in a regulatory loop.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Applied
Huifan Liu, Xinyue Zeng, Jiayong Huang, Xianhe Yuan, Qin Wang, Lukai Ma
Summary: Pomelo fruitlets have beneficial effects on glucose metabolism and blood sugar control due to their high dietary fiber content. The fibers also have the ability to decrease total cholesterol content and influence gut microbiota composition.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Zaneta Dziegelewska-Sokolowska, Alicja Majewska, Adam Prostek, Malgorzata Gajewska
Summary: The mammary gland consists of epithelial tissue and stroma, with preadipocytes and adipocytes as important components of the stroma. This study investigated the effects of paracrine factors secreted by preadipocytes and adipocytes on the development of bovine mammary epithelial cells. The results showed that factors secreted by fully differentiated adipocytes and preadipocytes can decrease apoptosis and increase the size of spheroids formed by the mammary epithelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Grace Yoon, Laurie A. Davidson, Jennifer S. Goldsby, Destiny A. Mullens, Ivan Ivanov, Sharon M. Donovan, Robert S. Chapkin
Summary: The study compared the transcriptional profiles of exfoliated cells with that of the ileal and colonic mucosa, finding that exfoliated cells in the distal colon may reflect mucosal signatures of more proximal regions of the gut.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Article
Cell Biology
Laura E. Sanman, Ina W. Chen, Jake M. Bieber, Veronica Steri, Coralie Trentesaux, Byron Hann, Ophir D. Klein, Lani F. Wu, Steven J. Altschuler
Summary: By investigating the responses of intestinal epithelium to different signaling pathways, it was found that modulation of transit-amplifying cell proliferation can change the ratio of differentiated cell types, highlighting the important role of transit-amplifying cells in tuning differentiated cell-type composition.
DEVELOPMENTAL CELL
(2021)
Article
Gastroenterology & Hepatology
Seungil Kim, Yun-Chan Shin, Tae-Young Kim, Yeji Kim, Yong-Soo Lee, Su-Hyun Lee, Mi-Na Kim, O. Eunju, Kwang Soon Kim, Mi-Na Kweon
Summary: Mucin-degrading bacteria, such as A. muciniphila, can promote the proliferation of intestinal stem cells and differentiation of specific cell types in the small intestine and colon. Additionally, treatment with A. muciniphila leads to higher levels of acetic and propionic acids in the cecal contents of mice. Furthermore, pre-treatment with A. muciniphila can reduce gut damage caused by radiation and methotrexate, suggesting a potential role in intestinal homeostasis maintenance.
Article
Agriculture, Dairy & Animal Science
Min Wang, Lixia Wang, Xian Tan, Lei Wang, Xia Xiong, Yancan Wang, Qiye Wang, Huansheng Yang, Yulong Yin
Summary: Intestinal epithelial homeostasis undergoes significant changes during early weaning in piglets, affecting intestinal morphology, cell turnover, and gene expression. The mTOR signaling pathway plays a crucial role in these developmental changes.
Article
Cell Biology
Justine Creff, Ada Nowosad, Anne Prel, Anne Pizzoccaro, Marion Aguirrebengoa, Nicolas Duquesnes, Caroline Callot, Thomas Jungas, Christine Dozier, Arnaud Besson
Summary: p57Kip2 is a cyclin/CDK inhibitor that regulates intestinal stem cell (ISC) fate and proliferation in a CDK-independent manner. In the absence of p57, there is increased proliferation of intestinal crypts and amplification of transit-amplifying cells and Hopx+ ISCs. RNA sequencing analyses reveal significant gene expression changes in Hopx+ ISCs upon p57 depletion. Furthermore, p57 binds to and inhibits the activity of Ascl2, a transcription factor critical for ISC specification and maintenance, by recruiting a corepressor complex. Hence, p57 plays a key role in maintaining Hopx+ ISC quiescence and repressing the ISC phenotype by inhibiting Ascl2 in a CDK-independent manner during intestinal development.
Article
Gastroenterology & Hepatology
Lea Juznic, Kenneth Peuker, Anne Strigli, Mario Brosch, Alexander Herrmann, Robert Haesler, Michael Koch, Liz Matthiesen, Yvonne Zeissig, Britt-Sabina Loescher, Alexander Nuber, Gunnar Schotta, Volker Neumeister, Triantafyllos Chavakis, Thomas Kurth, Mathias Lesche, Andreas Dahl, Anne von Maessenhausen, Andreas Linkermann, Stefan Schreiber, Konrad Aden, Philip C. Rosenstiel, Andre Franke, Jochen Hampe, Sebastian Zeissig
Summary: SETDB1 plays a crucial role in intestinal epithelial homeostasis, and its deficiency may lead to defects in differentiation and inflammation in the intestinal epithelium. Rare variants associated with loss of function in SETDB1 were found in some IBD patients, suggesting a potential contribution to the pathogenesis of intestinal inflammation.
Article
Immunology
Mingfang Pan, Nilakshi Barua, Margaret Ip
Summary: This study characterized the diversity of mucin-degrading bacteria in the human gut microbiota using a cultivation-based molecular profiling method. The results showed that these bacteria were widely distributed and more abundant than previously thought. Furthermore, these commensal bacteria exhibited anti-inflammatory effects and modulated the epithelial barrier function.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Brittany R. Jenkins, Nathan A. Blaseg, Heather M. Grifka-Walk, Benjamin Deuling, Steve D. Swain, Eric L. Campbell, Seth T. Walk, Douglas J. Kominsky
Summary: IL-10 signaling plays a crucial role in regulating intestinal mucosal homeostasis by modulating the balance of proliferation and differentiation in IECs. Depletion of IL-10 signaling leads to a shift towards goblet cell differentiation, increased proliferation, and minor changes in apoptosis. Further research is needed to explore the detailed mechanisms underlying these cellular processes.
Article
Pharmacology & Pharmacy
Matteo Centonze, Erwin J. W. Berenschot, Simona Serrati, Arturo Susarrey-Arce, Silke Krol
Summary: This study found that HT29 cells grown on a surface decorated with amorphous SiO2 structures showed more similarity with differentiated HT29-MTX cells than undifferentiated HT29 cells. These cells formed clusters and secreted mucus.
Letter
Multidisciplinary Sciences
Alan Herbert
Article
Neurosciences
Jan Wenzel, Josephine Lampe, Helge Mueller-Fielitz, Raphael Schuster, Marietta Zille, Kristin Mueller, Markus Krohn, Jakob Koerbelin, Linlin Zhang, Umit Ozorhan, Vanessa Neve, Julian U. G. Wagner, Denisa Bojkova, Mariana Shumliakivska, Yun Jiang, Anke Faehnrich, Fabian Ott, Valentin Sencio, Cyril Robil, Susanne Pfefferle, Florent Sauve, Caio Fernando Ferreira Coelho, Jonas Franz, Frauke Spiecker, Beate Lembrich, Sonja Binder, Nina Feller, Peter Koenig, Hauke Busch, Ludovic Collin, Roberto Villasenor, Olaf Joehren, Hermann C. Altmeppen, Manolis Pasparakis, Stefanie Dimmeler, Jindrich Cinatl, Klaus Pueschel, Matija Zelic, Dimitry Ofengeim, Christine Stadelmann, Francois Trottein, Ruben Nogueiras, Rolf Hilgenfeld, Markus Glatzel, Vincent Prevot, Markus Schwaninger
Summary: COVID-19 can cause damage to cerebral small vessels and neurological symptoms by infecting brain endothelial cells and cleaving NEMO through the viral protease M-pro. In animal models, the loss of NEMO leads to the generation of string vessels. Blocking RIPK3, a mediator of regulated cell death, can prevent vessel disruption caused by NEMO ablation, suggesting it as a potential therapeutic target.
NATURE NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Hannah Schuenke, Ulrike Goebel, Ivan Dikic, Manolis Pasparakis
Summary: OTULIN functions as a negative regulator of linear ubiquitination to suppress skin inflammation primarily by inhibiting RIPK1-mediated keratinocyte necroptosis. Deficiency of OTULIN leads to systemic inflammatory pathology and OTULIN-related autoinflammatory syndrome (ORAS) affecting multiple organs, including the skin. Additionally, skin inflammation in OTULIN deficiency is driven by TNFR1 signaling in keratinocytes and is dependent on RIPK1 kinase activity, with necroptosis and apoptosis playing redundant roles in the pathogenesis.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Mar Cabeza-Cabrerizo, Carlos M. Minutti, Mariana Pereira da Costa, Ana Cardoso, Robert P. Jenkins, Justina Kulikauskaite, Michael D. Buck, Cecile Piot, Neil Rogers, Stefania Crotta, Lynne Whittaker, Hector Huerga Encabo, John W. McCauley, Judith E. Allen, Manolis Pasparakis, Andreas Wack, Erik Sahai, Caetano Reis e Sousa
Summary: Protection from respiratory viruses like influenza A virus (IAV) requires T cell-mediated immune responses initiated by conventional dendritic cells (cDCs) in the respiratory tract. Reinforcing the resident lung cDC network by cDC progenitors is necessary for effective induction of T cell responses against IAV in mice. Chemokines produced during IAV infection recruit pre-cDCs from blood, directing them to infection sites to increase cDC numbers and amplify T cell responses.
SCIENCE IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ulrika C. Frising, Silvia Ribo, M. Giulia Doglio, Bernard Malissen, Geert van Loo, Andy Wullaert
Summary: Macrophages play an important role in driving CAPS in mice, while neutrophils act independently in propagating autoinflammation.
Article
Oncology
Vangelis Kondylis, Farina Schneider, Fabian Schorn, Nikos Oikonomou, Beate Katharina Straub, Sabine Werner, Philip Rosenstiel, Manolis Pasparakis
Summary: In the absence of autophagy defects, activation of p62 and Nrf2 exacerbates liver disease phenotype and can lead to early death. Expression of a p62 mutant exacerbates hepatocarcinogenesis, while forced activation of Nrf2 itself does not increase liver injury or tumor burden.
Article
Biochemistry & Molecular Biology
Dhruv Chauhan, Dieter Demon, Lieselotte Vande Walle, Oonagh Paerewijck, Annalisa Zecchin, Leslie Bosseler, Karin Santoni, Remi Planes, Silvia Ribo, Amelie Fossoul, Amanda Goncalves, Hanne Van Gorp, Nina Van Opdenbosch, Filip Van Hauwermeiren, Etienne Meunier, Andy Wullaert, Mohamed Lamkanfi
Summary: This study confirms the presence of multiple canonical inflammasomes in neutrophils and identifies their differential roles in neutrophil immune responses. It provides new insights into the immune mechanisms of neutrophils.
Article
Multidisciplinary Sciences
Huipeng Jiao, Laurens Wachsmuth, Simone Wolf, Juliane Lohmann, Masahiro Nagata, Goeksu Goekberk Kaya, Nikos Oikonomou, Vangelis Kondylis, Manuel Rogg, Martin Diebold, Simon E. Troeder, Branko Zevnik, Marco Prinz, Christoph Schell, George R. Young, George Kassiotis, Manolis Pasparakis
Summary: Mutations of the ADAR1 gene cause chronic activation of type I interferon responses, leading to severe diseases. The interaction between ADAR1 and another protein called ZBP1 prevents IFN activation. Deficiency or mutation of ZBP1's Z alpha domains reduces expression of IFN-stimulated genes and prevents early postnatal lethality in mice with mutated ADAR1 Z alpha domain.
Article
Multidisciplinary Sciences
Takashi Imai, Trieu-My Van, Manolis Pasparakis, Apostolos Polykratis
Summary: The canonical NF-kappa B signaling in smooth muscle cells (SMCs) plays a critical role in the development of atherosclerosis. SMC-specific ablation of NEMO, an essential subunit for NF-kappa B activation, was found to inhibit high fat diet induced atherosclerosis and reduce inflammation and plaque size in a mouse model. In vitro experiments also showed that NEMO-deficient SMCs exhibited reduced proliferation and migration in response to inflammatory stimulus.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Dmitry Kopelyanskiy, Chantal Desponds, Florence Prevel, Matteo Rossi, Romain Migliorini, Tiia Snaka, Remzi Onur Eren, Stephanie Claudinot, Lon-Fye Lye, Manolis Pasparakis, Stephen M. Beverley, Nicolas Fasel
Summary: Inducible nitric oxide synthase (iNOS) is involved in the control of intracellular human pathogens. Leishmania protozoan parasites carrying Leishmania RNA virus 1 (LRV1) induce low levels of iNOS and nitric oxide (NO), which fail to control infection. The induction of iNOS requires strong activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) triggered by LRV1-induced type I interferon (IFN). LRV1-bearing Leishmania parasites limit iNOS production by suppressing NF-kappa B activation. LRV1-induced iNOS may contribute to parasite metastasis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Takayuki Imanishi, Midori Unno, Natsumi Yoneda, Yasutaka Motomura, Miho Mochizuki, Takaharu Sasaki, Manolis Pasparakis, Takashi Saito
Summary: This study reveals that T cell-specific deficiency of RIPK1 in mice leads to premature T cell senescence and age-related diseases, resulting in premature death. RIPK1 deficiency activates mTORC1, leading to enhanced cytokine production, induction of senescence-related genes, and increased caspase-3/7 activation. The impaired phenotypes and responses are restored by combined deficiency of RIPK3 and caspase-8 inhibition.
Article
Allergy
Selina K. Jorch, Annika McNally, Philipp Berger, Jonas Wolf, Kim Kaiser, Andrian Chetrusca Covash, Stefanie Robeck, Isabell Pastau, Olesja Fehler, Saskia-L. Jauch-Speer, Sven Hermann, Michael Schafers, Hanne Van Gorp, Apurva Kanneganti, Joke Dehoorne, Filomeen Haerynck, Federica Penco, Marco Gattorno, Jae Jin Chae, Paul Kubes, Mohamed Lamkanfi, Andy Wullaert, Markus Sperandio, Thomas Vogl, Johannes Roth, Judith Austermann
Summary: This study provides evidence that S100A8/A9 alarmins are released through a pyrin/caspase-1/gasdermin D-dependent pathway in FMF patients, and they directly drive autoimmune inflammation in vivo.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Cell Biology
Elien Eeckhout, Lisa Hamerlinck, Veronique Jonckheere, Petra Van Damme, Geert van Loo, Andy Wullaert
Summary: This study showed that different types of cell death, including apoptosis, necroptosis, and pyroptosis, play different roles in host defense against C. rodentium infection in mice. While both necroptosis and pyroptosis were found to be dispensable for pathogen clearance, apoptosis and caspase-8 signaling were essential for intestinal host defense. The study also revealed the importance of canonical inflammasome signaling in systemic host defense against C. rodentium infection.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Karel F. A. Van Damme, Pieter Hertens, Arne Martens, Elisabeth Gilis, Dario Priem, Inge Bruggeman, Amelie Fossoul, Jozefien Declercq, Helena Aegerter, Andy Wullaert, Tino Hochepied, Esther Hoste, Lieselotte Vande Walle, Mohamed Lamkanfi, Savvas N. Savvides, Dirk Elewaut, Bart N. Lambrecht, Geert van Loo
Summary: This study provides experimental confirmation that protein citrullination and NET formation do not serve as pathogenic mechanisms in the development of RA and SLE pathology in mice with A20 mutations.
SCIENTIFIC REPORTS
(2023)
Meeting Abstract
Pharmacology & Pharmacy
J. Wenzel, J. Lampe, H. Mueler-Fielitz, K. Mueler, R. Schuster, M. Zille, L. Zhang, M. Krohn, V. Neve, H. Altmeppen, F. Sauve, M. Pasparakis, V. Prevot, R. Hilgenfeld, M. Glatzel, M. Schwaninger
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2021)