Article
Agriculture, Multidisciplinary
Pingping Zhou, Chunlei Yue, Yuchen Zhang, Yan Li, Xinyi Da, Xiuqi Zhou, Lidan Ye
Summary: This study constructed a rosmarinic acid producing Saccharomyces cerevisiae strain and achieved efficient biosynthesis through the removal of metabolic bottlenecks and tuning down gene expression. It provides a promising platform for fermentative production of rosmarinic acid.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Jorge El-Azaz, Francisco M. Canovas, Belen Barcelona, Concepcion Avila, Fernando de la Torre
Summary: The research revealed that a key step in the biosynthesis of Phenylalanine in plants experienced feedback de-regulation during evolution, leading to a relaxation of regulation in certain ADT enzymes and a significant accumulation of Phenylalanine in plant tissues, enabling the massive production of compounds like lignin.
Article
Biochemistry & Molecular Biology
Ryo Yokoyama, Marcos V. V. de Oliveira, Bailey Kleven, Hiroshi A. Maeda
Summary: The plant shikimate pathway directs carbon flow towards biosynthesis of aromatic amino acids and phytochemicals. The microbial shikimate pathway is feedback inhibited by amino acids at the first enzyme, while plant extracts generally do not inhibit the pathway. The study of Arabidopsis DHS enzymes reveals complex regulation of the shikimate pathway entry reaction and provides insights into controlling production of aromatic compounds.
Article
Plant Sciences
Heejin Yoo, Stuti Shrivastava, Joseph H. Lynch, Xing-Qi Huang, Joshua R. Widhalm, Longyun Guo, Benjamin C. Carter, Yichun Qian, Hiroshi A. Maeda, Joseph P. Ogas, John A. Morgan, Amy Marshall-Colon, Natalia Dudareva
Summary: The flux towards phenylalanine in plants is highly regulated, with ADT playing a key role; in petunia flowers with high phenylalanine levels, ADT regulation is relaxed; regulation of the d-ribulose-5-phosphate 3-epi merase gene in the pentose phosphate pathway controls flux into the shikimate pathway.
Article
Chemistry, Multidisciplinary
Roger M. Pallares, Dahlia D. An, Solene Hebert, Alex Loguinov, Michael Proctor, Jonathan A. Villalobos, Kathleen A. Bjornstad, Chris J. Rosen, Chris D. Vulpe, Rebecca J. Abergel
Summary: This study extensively characterizes the toxicity mechanisms of early lanthanides, lanthanum and praseodymium, through a multidimensional genome-wide toxicogenomic study using Saccharomyces cerevisiae as a model system. The findings identify key genes and proteins involved in the response to lanthanide-induced toxicity and suggest potential strategies for prophylaxis and therapeutics.
Article
Plant Sciences
Rodolfo A. Maniero, Alessandra Koltun, Marielle Vitti, Bruna G. Factor, Nathalia de Setta, Amanda S. Camara, Joni E. Lima, Antonio Figueira
Summary: In this study, a novel AMT transporter, ScAMT3;3, was identified in sugarcane and shown to play a crucial role in ammonium transport. These findings provide a basis for future research on improving sugarcane yield and nitrogen use efficiency.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Lihong Jiang, Chang Dong, Tengfei Liu, Yi Shi, Handing Wang, Zeng Tao, Yan Liang, Jiazhang Lian
Summary: A novel strategy was reported to improve the functional expression of P450 enzymes in Saccharomyces cerevisiae by screening three new target genes from an Arabidopsis thaliana cDNA library, resulting in increased production efficiency of natural products.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Patricia Benito, Daniele Ligorio, Javier Bellon, Lynne Yenush, Jose M. Mulet
Summary: This study evaluates the potential of different biostimulants using two different model organisms and explores the additive, synergistic or antagonistic effects of different combinations of biostimulants to design new formulations with enhanced effects on plant growth or tolerance to abiotic stress. The method enables a fast screening of various products in a combinatorial manner to develop novel formulations of natural extracts with biostimulant potential.
Article
Biotechnology & Applied Microbiology
N. Degenaar, B. Divol
Summary: This study assessed the physiological impact of exogenous supplementation of L-phenylalanine (Phe) on Kluyveromyces marxianus. The results showed that Phe exerted cytotoxic effects on the yeast, impacting amino acid metabolism and aroma production. Lipid supplementation supported viability maintenance and fermentation performance. The enhanced production of 2-phenylethyl acetate (2-PEA) may be a compensatory detoxification mechanism for this yeast.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Plant Sciences
Chengsong Zhao, Rejane Pratelli, Shi Yu, Brett Shelley, Eva Collakova, Guillaume Pilot
Summary: Amino acid transporters play a critical role in distributing amino acids within plant cells and organs. The study of UMAMIT family in plants reveals that these proteins are mainly exporters located at the tonoplast or plasma membrane, confirming their role in exporting amino acids from the cytosol for intra- and intercellular transport.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Jie Zhang, Robert Wysocki, Fangbai Li, Min Yu, Enrico Martinoia, Won-Yong Song
Summary: Arsenic is harmful to all living organisms, and plants have systems to regulate its uptake and translocation. Ubiquitination, a post-translational modification, plays a crucial role in arsenic detoxification in yeast, but its role in plants is not well understood. This opinion article reviews recent findings and proposes that ubiquitination is important for regulating arsenic transport in plants. Future research ideas are also suggested.
TRENDS IN PLANT SCIENCE
(2023)
Review
Microbiology
Jun Dai, Huili Xia, Chunlei Yang, Xiong Chen
Summary: 2-Phenylethanol is an important flavoring ingredient derived from L-phenylalanine through biotransformation, but its biosynthesis process is influenced by the complex regulation of L-Phe metabolism in Saccharomyces cerevisiae.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lijun Meng, Mengxue Diao, Qingyan Wang, Longyun Peng, Jianxiu Li, Nengzhong Xie
Summary: This study demonstrates the advantage of using a bi-functional phenylalanine/tyrosine ammonia lyase in the biosynthetic pathway of resveratrol, resulting in enhanced production of resveratrol in Saccharomyces cerevisiae. By integrating multi-copy biosynthetic pathway genes, improving metabolic flux, and deleting by-pathway genes, the resveratrol production in shake flasks reached 1155.0 mg/L in yeast extract peptone dextrose medium. Furthermore, a non-auxotrophic strain was tailored for resveratrol production in minimal medium without exogenous amino acid addition, achieving the highest reported titer of resveratrol (4.1 g/L) to date.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Jeff Y. Chen, Arishba Mumtaz, Eliana Gonzales-Vigil
Summary: This study explores the evolution and molecular determinants of substrate specificity in the 3-ketoacyl-CoA synthase (KCS) gene family in Populus trichocarpa. Functional characterization and mutagenesis experiments reveal two key regions that contribute to substrate specificity. Additionally, the study finds differing sensitivities to KCS inhibitors among duplicated paralogs, despite their sequence similarity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Asif Khan, Muhammad Waqas, Muhammad Tufail, Sobia Ahsan Halim, Waheed Murad, Syed Umair Ahmad, Muhammad Faheem, Jalal Uddin, Asaad Khalid, Ashraf N. N. Abdalla, Ajmal Khan, Ahmed Al-Harrasi
Summary: Suppressor of gamma response 1 (SOG1) is a member of the NAC domain family transcription factors involved in DNA damage response in plants. Mutations in SOG1 can lead to diseases and cell death. This study used computational approaches to identify deleterious nsSNPs in the SOG1 protein and assess their impact on structure and function.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Biotechnology & Applied Microbiology
Jacqueline MacDonald, Ketan Doshi, Marike Dussault, J. Christopher Hall, Larry Holbrook, Ginny Jones, Angelo Kaldis, Cassidy L. Klima, Phil Macdonald, Tim McAllister, Michael D. McLean, Andrew Potter, Alex Richman, Heather Shearer, Oksana Yarosh, Han Sang Yoo, Edward Topp, Rima Menassa
BIOTECHNOLOGY ADVANCES
(2015)
Article
Biotechnology & Applied Microbiology
Reza Saberianfar, Jussi J. Joensuu, Andrew J. Conley, Rima Menassa
PLANT BIOTECHNOLOGY JOURNAL
(2015)
Review
Biotechnology & Applied Microbiology
Edward Topp, Rebecca Irwin, Tim McAllister, Martin Lessard, Jussi J. Joensuu, Igor Kolotilin, Udo Conrad, Eva Stoeger, Tsafrir Mor, Heribert Warzecha, J. Chris Hall, Michael D. McLean, Eric Cox, Bert Devriendt, Andrew Potter, Ann Depicker, Vikram Virdi, Larry Holbrook, Ketan Doshi, Marike Dussault, Robert Friendship, Oksana Yarosh, Han Sang Yoo, Jacqueline MacDonald, Rima Menassa
BIOTECHNOLOGY ADVANCES
(2016)
Article
Plant Sciences
Sean Miletic, David J. Simpson, Christine M. Szymanski, Michael K. Deyholos, Rima Menassa
FRONTIERS IN PLANT SCIENCE
(2016)
Article
Plant Sciences
Reza Saberianfar, Amirali Sattarzadeh, Jussi J. Joensuu, Susanne E. Kohalmi, Rima Menassa
FRONTIERS IN PLANT SCIENCE
(2016)
Editorial Material
Biotechnology & Applied Microbiology
Reza Saberianfar, Rima Menassa
PLANT BIOTECHNOLOGY JOURNAL
(2017)
Article
Multidisciplinary Sciences
Laura L. Arthur, Joyce J. Chung, Preetam Jankirama, Kathryn M. Keefer, Igor Kolotilin, Slavica Pavlovic-Djuranovic, Douglas L. Chalker, Vojislava Grbic, Rachel Green, Rima Menassa, Heather L. True, James B. Skeath, Sergej Djuranovic
NATURE COMMUNICATIONS
(2017)
Article
Biochemical Research Methods
Sean Miletic, Martin Huenerberg, Angelo Kaldis, Jacqueline MacDonald, Antoine Leuthreau, Tim McAllister, Rima Menassa
BIOTECHNOLOGY JOURNAL
(2017)
Article
Plant Sciences
Jacqueline MacDonald, Sean Miletic, Typhanie Gaildry, Adam Chin-Fatt, Rima Menassa
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Plant Sciences
Mistianne Feeney, Maike Kittelmann, Rima Menassa, Chris Hawes, Lorenzo Frigerio
Article
Multidisciplinary Sciences
Kishor Duwadi, Ling Chen, Rima Menassa, Sangeeta Dhaubhadel
Article
Multidisciplinary Sciences
Amirali Sattarzadeh, Reza Saberianfar, Warren R. Zipfel, Rima Menassa, Maureen R. Hanson
SCIENTIFIC REPORTS
(2015)
Article
Biochemical Research Methods
Hong Hanh Tran, Bin Chen, Hui Chen, Rima Menassa, Xiuming Hao, Mark Bernards, Norman P. A. Huner, Aiming Wang
JOURNAL OF VIROLOGICAL METHODS
(2019)
Article
Biotechnology & Applied Microbiology
Hong Zhu, L. Bruce Reynolds, Rima Menassa
Article
Biochemical Research Methods
Lauri J. Reuter, Mohammad-Ali Shahbazi, Ermei M. Makila, Jamo J. Salonen, Reza Saberianfar, Rima Menassa, Helder A. Santos, Jussi J. Joensuu, Anneli Ritala
BIOCONJUGATE CHEMISTRY
(2017)
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)